• Journal of Plant Biotechnology
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• v.48 no.3
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• pp.193-200
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• 2021

Barley (Hordeum vulgare cv. Hangmaeg) sprouts are important microgreens that contain high levels of polyphenols and flavonoids, as well as minerals, vitamin and chlorophyll. Barley sprouts were grown for 9 days and growth was checked every 3 days. In this study, the cultivation efficiency according to the nutrient solution treatment was evaluated by analyzing the length of barley sprouts, fresh weight, chlorophyll, and the yield by growth period. In addition, we tried to increase the industrial applicability of germinated barley through analysis of inorganic component, total polyphenol and total flavonoid content of the extract, and functional substance analysis using HPLC. As a result, the growth rate of the nutrient solution treatment group was faster than that of the control group. When harvested on the 9th day of sowing, the nutrient solution treatment group showed a significant increase in yield compared to the control group. And the barley sprout extract of the nutrient solution treatment group had higher total flavonoid content and luteolin content. Also, the efficiency of water was higher than that of ethanol when extracting phenolics from barley sprouts. Therefore, this study suggests that nutrient input is effective for increasing polyphenol content and increasing production in barley sprout hydroponics.

• Journal of Bio-Environment Control
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• v.21 no.1
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• pp.12-19
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• 2012

This research was performed to determine the appropriate daily last irrigation time to enhance the plant growth and the water and fertilizer use efficiencies in coir bag culture for tomato plant. The time to finish the daily irrigation was set by 1, 2, 3 and 4 hours before the sunset. The water content in the substrate was greatly affected by the last irrigation time. The earlier the last time, the greater the daily fluctuation of water contents in the substrate. The daily irrigation times were not affected by using irrigation management system controlled by drainage electrodes or the physiochemical properties of coir. The growth characteristics were not significantly different among the treatments. The highest marketable yields were obtained in the treatment finishing two hours before sunset, and the lowest yields were obtained in the the treatment finishing 4 hours before sunset. Based on the result from surveying quantity of irrigated water for 128 days of the experiment period, the water and fertilizer use efficiencies were lowest in the treatment finishing 4 hours before sunset, and the highest in the treatment finishing 2 hours before sunset. In terms of plant growth, yields, water and fertilizer use efficiencies, 2 hours before sunset treatment was determined as the most economical and desirable irrigation schedule.

• Asian Journal of Turfgrass Science
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• v.23 no.1
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• pp.9-22
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• 2009

Due to increasing concerns over issues with both water quantity and quality for turfgrass use, research was conducted to determine the response of five warm-season turfgrasses to deficit irrigation and to gain a better understanding of relative drought tolerance. St. Augustinegrass(Stenotaphrum secundatum [Walt.] Kuntze.) cultivars 'Floratam' and 'Palmetto', 'SeaIsle 1' seashore Paspalum(Paspalum vaginatumSwartz.), 'Empire' zoysiagrass(Zoysia japonica Steud.), and 'Pensacola' bahiagrass(Paspalum notatum Flugge) were established in lysimeters in the University of Florida Envirotron greenhouse facility in Gainesville. Irrigation was applied at100%, 80%, 60%, or 40% of evapotranspiration(ET). Evaluations included: a) shoot quality, leaf rolling, leaf firing; b) leaf relative water content(RWC), soil moisture content, chlorophyll content index(CCI), canopy photosynthesis(PS); c) multispectral reflectance(MSR); d) root distribution; and e) water use efficiency. Grasses irrigated at 100% and 80% of ET had no differences in visual quality, leaf rolling, leaf firing, RWC, CCI, and PS. Grasses irrigated at 60% of ET had higher values in physiological aspects than grasses irrigated at 40% of ET. 'Sealsle 1' and 'Palmetto' had a deeper root system than 'Empire' and 'Pensacola', while 'Floratam' had the least amount of root mass. Photosynthesis was positively correlated with visual assessments such as turf quality, leaf rolling, leaf firing, and sensor-based measurements such as CCI, soil moisture, and MSR. Reducing the amount of applied water by 20% did not reduce turfgrass quality and maintained acceptable physiological functioning.

• Journal of Bio-Environment Control
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• v.25 no.1
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• pp.63-70
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• 2016

Water drainage from the open hydroponics often causes significant environmental pollution due to agrochemicals and loss of water and nutrients. The objectives of this study were to show the potential application of an irrigation schedule based on threshold values of volumetric substrate water content for tomato (Solanum lycopersicum L. 'Samsamgu') cultivation in a commercial hydroponic farm during spring to summer cultivation. This study was performed for minimizing effluent from coir substrate hydroponics using a frequency domain reflectometry (FDR) sensor-automated irrigation, as compared with an integrated solar-radiation (IR) and conventional timer-irrigation (TIMER) after transplanting. In results, no significant difference in daily irrigation volume was found among the treatments until 88 days after transplant (DAT). However, during the 88 to 107 DAT, the daily irrigation volume was in the order of IR (2125 mL) > TIMER (2063 mL) > FDR (1983 mL), and during the 108 to 120 DAT, it was in the order of IR (2000 mL) > TIMER (1664 mL) > FDR (1500 mL). The lowest drainage volume was observed in the FDR treatment with the order of IR (12~19%) > TIMER (4~12%) > FDR (0~7%) during the entire growing period. A lower irrigation volume in the FDR treatment after 88 DAT may be due to the sensor's detecting capacity for less water absorption by plant after completing fruit maturity with apical pruning and removal of lower leaves, while a higher irrigation volume in the IR treatment may be due to gradual increase in integrated solar-radiation amount as closer to summer season. There was no significant difference in plant growth and fruit yield among the treatments; however, a 11% and 18% of higher soluble sugar content was observed in the FDR than that of TIMER and IR treatment. respectively.

• Korean Journal of Plant Resources
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• v.30 no.2
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• pp.152-159
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• 2017

The purpose of this study was to obtain basic data for using Aronia as a functional food material. The composition of anthocyanin was characterized and quantitated by LC-MS/MS, HPLC, and UV-VIS spectrophotometer techniques, respectively. The anthocyanin content was analyzed by temperature, time, pH, and the addition of citric acid. The UV-VIS spectrophotometer used for analysis of anthocyanin is less accurate than the LC-MS/MS method used in recent years. In the past, cyanidin-3-Glucoside was reported to be a major anthocyanin that contains Aronia. However, LC-MS/MS analysis in this study confirmed cyanidin-3-galactoside to be the major compound. The anthocyanin content of the Aronia powder began to decrease sharply at a temperature of $65^{\circ}C$ or higher when heated for 24 hours. In an aqueous solution of Aronia, the anthocyanin content was reduced by 50% at $65^{\circ}C$ for 10 hours and decreased by 85% at $85^{\circ}C$ within 10 hours. Above pH 8, the anthocyanin content was reduced by more than 50%. The results of this study will provide useful information to maintain anthocyanin content in the manufacturing process of Aronia. It could also be used to ensure the stability of anthocyanins in similar species of berries.

• Horticultural Science & Technology
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• v.29 no.6
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• pp.617-622
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• 2011

The study was conducted to determine the efficiency in producing spontaneous polyploids in some mandarin hybrids with different seed embryony. Seed formation by open pollination, frequency of spontaneous polyploids, and plant growth characteristics were evaluated in four mandarin hybrids with polyembryony such as 'Amakusa', 'Haruka', 'Hayaka', and 'Seminole' and two with monoembryony such as 'Benibae' and 'Harehime'. The mean number of the developed seeds per fruit was 10.0 and frequency of small seeds was 25.1%. Polyploids were selected from plants germinated in vitro by a flow cytometry and confirmed by chromosome analysis. One triploid was produced from 'Harehime', one tetraploid, 'Amakusa', and one tetrapoid, 'Benibae'. There were little differences in leaf shape, thickness, petiole length, and internode length between diploids and polyploids such as tri- or tetraploid. However, polyploids had larger stomata and lower density of stomata in abaxial epidermis than diploids. SPAS indicating chlorophyll content and photosynthetic rate were significantly affected by ploidy level. The results indicated that spontaneous polyploids might be produced by open pollination in some mandarin hybrids and monoembryony had higher frequency in polyploid occurrence than polyembryony.

• Journal of Korean Society of Forest Science
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• v.102 no.4
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• pp.601-607
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• 2013

The purpose of this study was to identify the effects of ozone pollution on the one woody species and two indoor plants in controlled environment. Pinus densiflora, Spathiphyllum patinii and Epipremnum aureum seedlings were exposed in both control and ozone chambers to investigate photosynthetic rate, water use efficiency, antioxidative enzyme activities such as GR(Glutathione reductase) and APX(Ascorbate peroxidase) activity and leaf anatomical response. Ozone was fumigated 8 hours for a day with 30 ppb concentration for 50 days. Pinus densiflora seedlings showed no significant difference on photosynthetic rate, water use efficiency, antioxidant enzyme activity during ozone exposure. Ozone concentration (30 ppb in this study) is not high enough to generate ozone damage on Pinus densiflora species. In contrast, ozone generally altered photosynthetic rate, antioxidant enzyme (especially GR) activity and leaf anatomy in two indoor species (Spathiphyllum patinii and Epipremnum aureum) exposed in ozone chamber were significantly differ from those of control in every measurement. These data suggest that two indoor species(Spathiphyllum patinii and Epipremnum aureum) are more sensitive to ozone than Pinus densiflora.

• Horticultural Science & Technology
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• v.29 no.4
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• pp.352-357
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• 2011

Rose seed shows low germination percentages (about 20%) because it has a high amount of substances that inhibit germination in the pericarp. We investigated the effect of orientation of achene, the day after pollination (DAP), and cold storage with or without half-cut in achene for the germination percentage in R. rugosa. Germination percentages of intact or half-cut achenes were investigated in a 16-hour photoperiod at $25^{\circ}C$ room on basal MS medium for two weeks. In germination percentage, maximum 100% was measured within one week when half-cut achenes were cultured on an orientation that the embryos facing to the light. Half-cut achenes at 90 DAP were germinated 100% regardless of cold storage. Various LED lights (red, blue, yellow, green, and white) were illuminated over the half-cut achenes to gain the effect of light color. Germination percentage of R. rugosa seeds under blue LED reached the greatest with 90% within one week of culture and these seedlings were the best with a steady growth rate. It is concluded that half-cut achenes would be an effective method to improve seed germination in R. rugosa without stratification or scarification. This system could be applied to breeding studies in rose cultivars.

• KSBB Journal
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• v.15 no.4
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• pp.346-351
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• 2000

Several solvents or combinations of solvents were tested for the extraction of wet or dried biomass at different extraction mode from plant cell cultures. Methanol gave the highest paclitaxel recovery with the least amount of solvent usage. before extraction drying of biomass wass helpful to decrease solvent usage in extraction step./ in this case drying method was very important to obtain high yield from dried biomass. In thid mode of operation counter-current extraction process can be able to decrease solvent usage but paclitaxel recovery was almost same with both batch and counter-current mode of operation. The number of extraction times was at least four to obtain high yield(>99%) from cell and one to obtain highyield(>96%) from cell debris in batch mode. Equilibrium (i.e. the ratio of paclitaxel in biomass to paclitaxel in the extraction solvent) was reached within 5 minutes. The minimum methodal concentration (90%) and solvent amount(biomass : solvent=1 Kg : 1L) are enough to obtain high yield(>98%) for extraction from biomass.

• Journal of Korean Society of Forest Science
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• v.113 no.2
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• pp.198-209
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• 2024

This study aimed to investigate the impact of light intensity, manipulated through different shading levels, on the growth and physiological responses of Thermopsis lupinoides. To assess the effects of shading treatments, we examined leaf mass per area, chlorophyll content, chlorophyll fluorescence response, and photosynthetic characteristics. T. lupinoidesexhibited adaptive responses under low light conditions (50% shading), showing increased leaf area and decreased leaf mass per area as shading levels increased. These changes indicate morpho-physiological adaptations to reduced light availability. At 50% shading, the physiological and ecological responses were favorable, with optimal photosynthetic functions including chlorophyll content, photosynthesis saturation point, photosynthetic rate, carbon fixation efficiency, stomatal conductance, transpiration rate, and water use efficiency. However, at 95% shading, the essential light conditions for growth were not met, significantly impairing photosynthetic functions. Consequently, 50% shading was determined to be the most optimal condition for T. lupinoides growth. These findings provide valuable insights for effective ex-situconservation practices and site selection for T. lupinoides, serving as foundational data for habitat restoration efforts.