• The Journal of the Convergence on Culture Technology
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• v.9 no.4
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• pp.587-592
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• 2023

Plant growth is regulated by a variety of factors, including organic matter availability. Organic nutrients are carbohydrate molecules from photosynthetic products produced by tissues associated with carbon and energy fixation called "sources". These compounds flow through plant vascular bundles into non-photosynthetic or growing tissues called "sinks". Among these possible compounds, the disaccharide fructosyl glucose, sucrose, is the most representative. During the transport of sucrose, the pathway from the source to the sinks can include hydrolysis of sucrose into glucose and fructose derivatives or direct transfer of sucrose. Among the enzymes involved in this, β-D-fructofuranosidase is the most important. Soluble neutral β-D-fructofuranosidase, one of several isoenzymes, is located in intracellular protoplasts and helps plant cells metabolize sucrose to produce energy. In order to track the activity of this enzyme during the course of plant growth, histological methods were used for the most effective immunolocalization. As a result, the activity was higher in the phloem and epidermis than in the mesophyll tissue in the leaf. In the growing stem, activity was high in the phloem, epidermis, and cortex. The activity of the root, which is a sink tissue, was high in all parts, but especially the highest in the root tip part. It is thought that this is because it helps unloading of sucrose in sink tissues that require sucrose degradation and plays a role in hydrolysising sucrose.

• Journal of Life Science
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• v.17 no.7 s.87
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• pp.983-989
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• 2007

Bacillus lichemiformis Kll, a plant growth promoting rhizobacterium was reported as a producer of auxin, siderophore, as well as antifungal cellulase under some culture conditions. In vitro test, B. licheniformis Kll represented excellent antagonistic ability against Fusarium oxyspoum (KACC 40037), and showed broad spectrum against other phytopathogenic fungi. B. licheniformis Kll had cellulolytic activity toward not only carboxymethyl-cellulose (CMC) but also insoluble cellulose, such as fungal cell wall cellulose, filter paper (Whatman No. 1), and Avicel. In addition, we confirmed antifungal substance production by butanol-extract methods. The strain produced optimally the antifungal substance when it was cultivated at pH 9.0, 30${\circ}$C for 4 days on nutrient medium. The biological control mechanisms of B. lichemiformis Kll were caused by antifungal substance, cellulase and siderophore against phytopathogenic fungi.

• Proceedings of the Korean Society of Broadcast Engineers Conference
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• 2022.06a
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• pp.186-189
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• 2022

스마트팜 피노믹스 시스템은 재배하는 식물의 성장조건에 맞게 생육 환경을 일정하게 유지하고 관리하는 장치이지만, 그럼에도 불구하고 식물의 질병은 여러 가지 이유로 발생할 수 있다. 본 논문에서는 스마트팜 피노믹스 시스템에서 Mean Shift Segmentation 을 통한 식물의 질병을 자동으로 검출하는 식물 질병 검출 알고리즘을 제안한다. 식물의 질병 정도가 임의의 임계값을 넘을 경우, 해당 식물을 질병의 정도가 심한 식물로 판별하고, 적절한 수확시기를 결정하여 더 나은 상품성을 가진 식물을 재배할 수 있는 방법을 제시한다. 또한 식물의 질병이 급격하게 심해지는 기간을 확인하여 인간의 개입 없이 완전히 자동화된 시스템으로 더욱 세심하고 효율적인 식물 재배를 가능하게 함을 제시한다. 본 논문에서는 아이스버그(양상추)에 대한 재배 환경을 구축하여 생장 기간에 아이스버그에 발생하는 질병인 팁번 현상을 검출하는 실험을 진행하였다. 본 논문에서 제안한 방법은 다른 종류의 다양한 식물에서도 질병 검출이 가능하며, 스마트팜 피노믹스 시스템에서 질병 검출의 자동화를 위한 한 가지 방법으로 활용될 수 있을 것으로 기대된다.

• Proceedings of the Korean Environmental Sciences Society Conference
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• 2004.05a
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• pp.252-254
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• 2004

수영천의 식물상은 모두 38과 68속 82분류군으로 조사되었는데 수영천 본류에서는 75분류군, 온천천에서는 42분류군으로 조사되어 온천천보다는 수영천 본류에서 많은 다양한 종이 출현하였다. 수영천에서 조사된 식물 82종류중 국화과(Compositae) 식물이 10속 14종(17.1%)으로 가장 많이 조사되었고 다음으로 화본과(Gramineae) 식물이 5속 6종(7.3%), 마디풀과 (Pologonaceae) 식물이 2속 5종, 십자화과(Cruciferae) 식물이 5속 5종, 콩과(Leguminosae) 식물이 5속 5종으로 각 6.1%를 차지하으며 기타 41속 47종(57.3%)으로 조사되었는데 다른 하천변 식물의 출현양상과도 유사함을 보였다. 또한 조사된 식물 중 대부분은 수영천 본류의 하천변에서 성장하고 있었으며 온천천에서는 꽃창포(Iris ensatra)를 비롯한 7분류군이 식재되어 외관상 보기에 좋도록 조성되어져 있었고 돼지풀(Ambrosia artemisiifolia var. elatior)을 비롯한 12분류군의 귀화식물이 대부분 온천천에서 자라고 있었다. 도시화의 척도로서 이용되는 도시화 지수(UI)는 수영천 본류에서 25.3%, 온천천에서 28.6%로 다른 하천변의 도시화 지수보다 높게 조사되었다.

• The Korean Journal of Mycology
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• v.21 no.2
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• pp.112-119
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• 1993

Various microorganisms secreting plant growth regulators were screened from the 100 microbial isolates including bacteria, actinomycetes and fungi. The isolates showing distict influence on the plant growth were identified as Aspergillus niger. The germinations of Raphanus and Cucubis seeds were completely inhibited by the culture filtrates of A. niger KK, A. niger KKS and A. niger ATCC 9462. The culture filtrates of the three strains also inhibited the formation and development of roots and hypocotyls of Raphanus. The culture filtrates of A. niger ATCC 26550 induced the hypocotyl curvature of Raphanus like plant hormone-auxin and at the same time caused the necrosis of the whole leaves.

• KSBB Journal
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• v.17 no.2
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• pp.203-206
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• 2002

The extracts of a lot of (different) plants were studied for inducing the secretion of growth hormone and IGF-1. In the cell test, it was found that the concentration of rat growth hormone was 88 - 99 ng/mL in a certain plant extract treatment group which was at least 6 times higher than the amount in control group. The concentration of IGF-1 in blood was 1,140ng/mL at 8 hours after the oral administration of YGF, which was remarkably much higher than that in control group. This meant that YGF maintained the level of IGF-1 secretion higher in the treated group. In addition, the increase of 6% in bone length was shown for the long-term oral administration of YGF. Thus, YGF seemed to affect significantly for inducing the secretion of IGF-1 which was essential for the physical growth and anti-aging.

• Korean Journal Plant Pathology
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• v.3 no.3
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• pp.198-202
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• 1987

Toxic metabolites ('Toxins'), produced by Helminthosporium sativum causing leaf blotch in barley and root rot in barley and wheat were partially purified through C-18 column. The partially purified toxins appeared heat unstable and lipophilic. The responses of toxins to wheat and barley root corresponded with those to lettuce growth with the different concentrations. The determination of the concentration of toxins produced was developed using the dilution end-points. The equation [Y = a log X + b) was obtained from the semi-log­graphy with the linear analysis. The values 'a' and 'b' were discussed with the responses of several plants on the toxin produced by H. sativum.

• Journal of the Korean Society of Food Science and Nutrition
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• v.34 no.6
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• pp.743-749
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• 2005

Estrogen is known to play an important role in maintaining bone mass, since the concentration of serum estrogen decrease after menopause and the estrogen deficiency results in bone loss. Phytoestrogens are plant compounds with estrogen-like biological activity, In this study, to investigate the bioactivities of phytoestrogen, which act on bone metabolism, we examined the effect of selected food-borne phytoestrogens (genistein, daidzein and resveratrol) on osteoblast proliferation and IGF-I production using MC3T3-El cells, a mouse calvaria osteoblast-like cell line. Cells were cultured in a serum free medium for 48 hr in the presence of genistein $(10^{-5}\;M)$, daidzein $(10^{-5}\;M)$ and resveratrol $(10^{-5}\;M)$. The effects of genistein, daidzein and resveratrol on the cell proliferation and growth were evaluated by total cell numbers, MTS assay and cell migration assay. Their effect was compared with the $17\beta-estradiol$. Genistein, daidzein and resveratrol exhibited stimulatory effects on the growth of MC3T3-El cells, and the most pronounced effect was shown with daidzein. In addition, these phytoestrogen increased alkaline phosphatase activity of MC3T3-El cells. These effects were similar to that of $17\beta-estradiol$ effects. Moreover, treatment with genistein, daidzein and resveratrol increased production of insulin like growth factor-I (IGF-I) in conditioned media, indicating that the growth promoting effects of these phytoestrogen were related to the changes in production of IGF-I by MC3T3-El cells. These results show that genistein, daidzein and resveratrol have a stimulatory effect on osteoblast function, and that these findings in a cell model may prove relevant to protecting against the loss of bone mass and the development of osteoporosis in human subjects.

• Korean Journal of Ecology and Environment
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• v.41 no.3
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• pp.412-421
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• 2008

Nutrient limitation was examined for the Dongbak Lake based on bioassay experiments and analyses of long-term monitoring data. From the results of data analyses, molar ratios of TN/TP and DIN/DIP were higher than 16 : 1 suggesting that phytoplankton growth was potentially limited by phosphorus. Phytoplankton responded to the addition of phosphates in the incubation experiments for all seasons whereas phytoplankton did not respond to other nutrients such as ammonia, nitrate and silicates. Size fraction (net and nano size) of phytoplankton also responded to the addition of phosphorus indicating that phytoplankton growth was limited by phosphorus in the Dongbok Lake. There was also a taxonomic shift from euglenophyceae to bacillariophyceae after addition of phosphate during warm season especially.

• Proceedings of the Plant Resources Society of Korea Conference
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• 2019.04a
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• pp.37-37
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• 2019

벼 재배포장에서 화학약제의 반복적인 사용으로 인한 병원균의 약제내성과 환경오염은 커다란 문제가 되고 있다. 이러한 약제내성을 극복하기 위한 노력의 하나로 유전자수준의 약제내성기작의 규명과 대체물질의 발굴이 필요하다고 사료된다. 이에 본 연구에서는 식물종이 지니는 광범위한 다양성에 주목하였고, 700여 종의 식물추출물을 벼 도열병균에 처리해 균사성장억제효과를 조사하는 선별실험을 실시하였고, 영릉향과 지모추출물이 높은 균사성장억제효과를 보이는 것을 확인했다. 영릉향과 지모 추출물이 화학약제를 대체하는 대안물질로서 가능성을 조사하기위해 균사성장억제능력 확인실험, 분획, HPLC (High Performance Liquid Chromatography), 포자발아 및 부착기 형성확인검정, ABC transporter의 발현조사, 환경독성실험등을 실시했다. 실험 결과, 영릉향과 지모 추출물을 벼 도열병균에 처리하였을 때 약제저항성유전자 ABC transporter의 기능이 저하되고 화학약제에 대한 내성이 감소를 확인했으며, 분획과 HPLC분석을 통해 영릉향과 지모의 유효성분을 확인했다. 또한, 실제 포장에서 영릉향 추출물과 지모 추출물을 사용하여 벼 도열병 방제가를 확인하는 포장시험을 실시한 결과, 각각 63%와 62%의 목 도열병 방제가를 확인 하였으며, 인축환경에 대한 유해성을 조사하기 위해 어류, 설치류, 중치류, 곤충을 대상으로 영릉향 추출물을 처리해 급성독성시험 실시한 결과, 고농도의 투여량에서도 독성이 없는 것을 확인했다. 위의 실험 결과를 토대로, 영릉향과 지모추출물이 화학농약에 의한 환경오염을 막을 수 있고, 인축과 환경에 피해를 입히지 않는 친환경자재로 친환경적인 생물농약의 신소재로서 가능성을 나타냈다고 사료된다.