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Production of Poly (3-Hydroxybutyrate-co-3-Hydroxyvalerate) by Bacillus sp. EMK-5020 Using Makgeolli Lees Enzymatic Hydrolysate and Propionic Acid as Carbon Sources (막걸리 주박 가수분해 산물과 propionic acid를 탄소원으로 이용한 Bacillus sp. EML-5020 균주로부터 poly (3-hydroxybutyrate-co-3-hydroxyvalerate) 생합성)

  • Kwon, Kyungjin;Kim, Jong-Sik;Chung, Chung-Wook
    • Journal of Life Science
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    • v.32 no.7
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    • pp.510-522
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    • 2022
  • In this study, to biosynthesize PHA with properties more similar to polypropylene, a Bacillus sp. EMK-5020 strain that biosynthesized poly (3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV) was isolated from soil. Bacillus sp. EMK-5020 strain biosynthesized PHBV containing 1.3% 3-hydroxyvalerate (3HV) using reducing sugar contained in Makgeolli lees enzymatic hydrolysate (MLEH) as a single carbon source. As the amount of propionic acid, which was added as a second carbon source, increased, the content of 3HV also increased. PHBV containing up to 48.6% of 3HV was synthesized when 1.0 g/l of propionic acid was added. Based on these results, the strain was cultured for 72 hr in a 3 l fermenter using reducing sugar in MLEH (20 g/l) and propionic acid (1 g/l) as the main and secondary carbon sources, respectively. As a result, 6.4 g/l DCW and 50 wt% of PHBV (MLEH-PHBV) containing 8.9% 3HV were biosynthesized. Through gel permeation chromatography and thermogravimetric analysis, it was confirmed that the average molecular weight and the decomposition temperature of MLEH-PHBV were 152 kDa and 273℃, respectively. In conclusion, the Bacillus sp. EMK-5020 strain could biosynthesize PHBV containing various 3HV fractions when MLEH and propionic acid were used as carbon sources, and PHBV-MLEH containing 8.9% 3HV was confirmed to have higher thermal stability than standard PHBV (8% 3HV).

A Study on Training Dataset Configuration for Deep Learning Based Image Matching of Multi-sensor VHR Satellite Images (다중센서 고해상도 위성영상의 딥러닝 기반 영상매칭을 위한 학습자료 구성에 관한 연구)

  • Kang, Wonbin;Jung, Minyoung;Kim, Yongil
    • Korean Journal of Remote Sensing
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    • v.38 no.6_1
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    • pp.1505-1514
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    • 2022
  • Image matching is a crucial preprocessing step for effective utilization of multi-temporal and multi-sensor very high resolution (VHR) satellite images. Deep learning (DL) method which is attracting widespread interest has proven to be an efficient approach to measure the similarity between image pairs in quick and accurate manner by extracting complex and detailed features from satellite images. However, Image matching of VHR satellite images remains challenging due to limitations of DL models in which the results are depending on the quantity and quality of training dataset, as well as the difficulty of creating training dataset with VHR satellite images. Therefore, this study examines the feasibility of DL-based method in matching pair extraction which is the most time-consuming process during image registration. This paper also aims to analyze factors that affect the accuracy based on the configuration of training dataset, when developing training dataset from existing multi-sensor VHR image database with bias for DL-based image matching. For this purpose, the generated training dataset were composed of correct matching pairs and incorrect matching pairs by assigning true and false labels to image pairs extracted using a grid-based Scale Invariant Feature Transform (SIFT) algorithm for a total of 12 multi-temporal and multi-sensor VHR images. The Siamese convolutional neural network (SCNN), proposed for matching pair extraction on constructed training dataset, proceeds with model learning and measures similarities by passing two images in parallel to the two identical convolutional neural network structures. The results from this study confirm that data acquired from VHR satellite image database can be used as DL training dataset and indicate the potential to improve efficiency of the matching process by appropriate configuration of multi-sensor images. DL-based image matching techniques using multi-sensor VHR satellite images are expected to replace existing manual-based feature extraction methods based on its stable performance, thus further develop into an integrated DL-based image registration framework.

A study of poverty experiences among Korean elderly women in the United States (재미 한인 여성노인의 빈곤경험에 관한 연구)

  • Yeom, Jihye
    • 한국노년학
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    • v.40 no.4
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    • pp.801-821
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    • 2020
  • There are a number of prior studies on the poverty experience of Korean women, but little is known about the poverty experience of Korean elderly women in the U.S. The purpose of this study is to examine the poverty experiences of Korean elderly women who immigrated to the U. S. Qualitative case study methods were used to achieve these research objectives. Three Korean elderly women living in Oakland of California who received Supplemental Security Income (SSI) from the U.S. federal government were included in the study. The data were collected by conducting a total of six meetings per participant, and the researcher read the consent form directly to the participants and obtained a hand-written signature. The analysis and interpretation began by repeating the interview transcript several times, and the repeated keywords were to be understood in the context, focusing on time, space, and relationships with other people. The contextual understanding of Korean elderly women's experiences in poverty was interpreted in three dimensions: extending poverty in their mother country, double torture as female immigrants, and limiting labor due to aging and diseases. Before moving to the U.S., they had a difficult livelihood by farming and one of them had to live in poverty due to the bereavement to her husband. But even after moving to the U.S., they have continued to live in poverty. As female immigrants with low education and no special skills, they were incorporated into the periphery of the labor market in the industrialized U.S. and were forced to make a living with low wages. Korean elderly women were unable to return to the labor market in the surrounding areas due to aging and diseases, and were continuing their impoverished lives relying on SSI. From the findings, we discussed the role of the Korean immigrants community as a way to improve the quality of life for Korean elderly women in the U.S.

Effects of Conflict Management Strategy Within Supply Chain on Partnership and Performance (공급망 내 갈등관리전략이 파트너십과 성과에 미치는 영향)

  • Ham, Yoon-Hee;Song, Sang-Hwa
    • Korean small business review
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    • v.42 no.1
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    • pp.79-105
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    • 2020
  • While individual enterprises with different objectives each other within supply chains require a variety of resources to achieve their own seeking goals and performances, it is necessary to form interdependent relationships among the enterprises to secure the resources what they need, as the individual enterprises are supposed to have limitations on such as time, space and cost to secure all the resources. In this process, conflict possibilities rise and opportunistic behaviors increase due to those environmental factors such as unbalanced information among enterprises, limited rationality, pursuit of interests, and risk aversion. Those existing studies on conflicts in the field of supply chains have limitations in that they failed to present specific conflict management strategies based on the conflict types from the perspective of the conflict resolution mechanism as the studies have made only focused on investigating the causes of conflicts and the impact of conflicts on performance. In this study, therefore, it used the TKI model of Kilmann and Thomas(1977) to subdivide the conflict management strategies in the process of transactions within supply chains by enterprises, and looked into the impact on partnership and performance according to each strategy. As the results, it showed that those types of conflict management strategies such as concession type and cooperation type had a positive(+) impact on the relationship commitment as a factor of partnership, and it was identified that the relationship commitment had a positive(+) impact on performance. In other words, it can be considered that the enterprises making use of the concession type & the cooperation type conflict management strategies under the situation of conflict would be able to have a very positive impact on their performances if they can make good relationship commitment such as investments in and efforts for the sustainable relationship along with the conflict management, while recognizing the importance of relationship. The most important meaning of this study lies on in terms of that it would be contributable to strengthening the partnership between enterprises and minimizing the risk of supply chains caused by conflicts through these results from the study.

The Use of Transmedia in Current Affairs Radio Shows Focusing on 'That Honey Show' of Kim Hyun-Jung's News Show(CBS) (라디오 시사프로그램의 트랜스미디어 활용 연구 - CBS <김현정의 뉴스쇼-댓꿀쇼>를 중심으로 -)

  • Shin, Jung-Ah;Han, Hee-Jeong
    • Journal of Korea Entertainment Industry Association
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    • v.15 no.6
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    • pp.35-54
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    • 2021
  • This study examines the characteristics of CBS's Kim Hyun-Jung's News Show(hearafter News Show) and the change to transmedia. The distinguishing characteristics of News Show compared to radio-based current affairs programs are as follows. First, despite its time limit, it adopts the form of investigative reports or investigative documentaries to uncover the truth of the events through 'Detective Son Su-Ho', etc. Second, News show's interviews have avoided biased stance in the reporting of minority issues by interviewing the affected parties anonymously including people involved in incidents and accidents, bereaved families, and other victims. News Show has been producing a transmedia content called 'That Honey Show' (a show that reads comments as fun as honey) since November 2018. 'That Honey Show' is broadcast in real time on YouTube right after the News Show radio broadcast ends. As a form of spin-off content, 'That Honey Show' breaks down the boundaries among staff, MCs, and guests, as well as shifting roles by using 'vice characters'. The female host, Kim conducts interviews with the main characters related to various issues and extends the fixed identity of current affairs shows to the everyday politics and cultural realms. Thus she draws active participation and responses audience. This paper analyzes two representative broadcast cases of 'That Honey Show'-first, the case of resistance and activity of the BTS fandom ARMY in the US presidential election, and, second, the case of reporting on the Nth room incident. This analysis considers the critical participation of digital citizens and the effect of fostering a sense of community in the current affairs show in the transmedia era.

An Exploratory Study on the Business Failure Recovery Factors of Serial Entrepreneurs: Focusing on Small Business (연속 기업가의 사업 실패 회복요인에 관한 탐색적 연구: 소상공인을 중심으로)

  • Lee, Kyung Suk;Park, Joo Yeon;Sung, Chang Soo
    • Asia-Pacific Journal of Business Venturing and Entrepreneurship
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    • v.16 no.6
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    • pp.17-29
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    • 2021
  • Recently, as social distancing have been raised due to the re-spread of COVID-19, the number of serial entrepreneurs who are closing their business is rapidly increasing. Learning from failure is a source of success, but business failure can result in psychological and economic losses and negative emotions of the serial entrepreneur. At this point, it is very important to find a way to recover the negative emotions caused by business failures of serial entrepreneurs. Recently, a strategic model has emerged to deal with the negative emotions of grief caused by business failures of serial entrepreneurs. This study identified the recovery factors from the grief of business failures of serial entrepreneurs and analyzed Shepherd's(2003) three areas: loss orientation, restoration orientation, and dual process. To this end, individual in-depth interviews were conducted with 12 small business serial entrepreneurs who challenged re-startup to identify the attributes of recovery factors that were not identified with quantitative data. As a result of the study, first, recovery factors were investigated in three areas: individual orientation, family orientation, and network orientation. It was found to help improve recovery in nine categories: self-esteem, persistence, personal competence, hobbies, self-confidence, family support, networks, religion, and social support. Second, recovery obstacle factors were investigated in three areas: psychological, economic, and environmental factors. Nine categories including family, health, social network, business partner, competitor, partner, fund, external environment, and government policy were found to persist negative emotions. Third, the emotional processing process for grief was investigated in three areas: loss orientation, restoration orientation, and dual process. Ten categories such as family, partner support, social member support, government support, hobbies, networks, change of business field, moving, third-party perspective, and meditation were confirmed to enhance rapid recovery in the emotional processing process for grief. The implications of this study are as follows. The process of recovering from the grief caused by business failures of serial entrepreneurs was attempted by a qualitative study. By extending the theory of Shepherd(2003), This study can be applied to help with recovery research. In addition, conceptual models and propositions for future empirical research were presented, which can be discussed in carious academic ways.

A study on the simulation method for the flushing flowrate and velocity in the watermain using a hydrant and a drain valve (소화전과 이토변을 이용한 플러싱 적용 시 관 내 세척유량과 유속 모의 방안에 관한 연구)

  • Gim, ARin;Lee, Eunhwan;Lee, SongI;Kim, kwang hyun;Jun, Hwandon
    • Journal of Korea Water Resources Association
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    • v.55 no.spc1
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    • pp.1251-1260
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    • 2022
  • Recently, due to the deterioration of watermains and the detachment of scale which is accumulated on the watermain surface, water quality accidents in a water supply network occur frequently. As scale accumulated on watermains is stabilized, it may not cause water quality accidents under the normal operating condition. However, due to water hammer or transient flow caused by the abrupt velocity and/or direction of flow change, it can be detached from the watermain surface resulting in water quality accidents. To prevent these kinds of water quality accidents, it is required to remove scale by watermain cleaning regularly. Many researches about flushing which is the most popular water cleaning method are focused on the desirable velocity criteria and the cleaning condition to accomplish the effect of flushing whereas less amount of research effort is given to develop a method to consider whether the desirable velocity for flushing can be obtained before flushing is performed. During flushing, the major and minor headloss is occurred when flushing water flows through a hydrant or drain valve. These headloss may slow down the velocity of flushing water so that it can reduce the flushing effect. Thus, in this study, we suggest a method to simulate the flow velocity of flushing water using "MinorLoss Coefficient" and "Emitter Coefficient" in EPANET. The suggested method is applied to a sample network and the water supply network of "A" city in Korea to compare the flushing effect between "flushing through a hydrant" and "flushing through a drain valve". In case of "flushing through a hydrant", if the hydraulic condition ocurring from a watermain pipe connecting to the inlet pipe of a hydrant to the outlet of a hydrant is not considered, the actual flowrate and velocity of a flow is less than the simulated flowrate and velocity of a flow. In case of "flushing through a drain valve", the flushing velocity and flowrate can be easily simulated and the difference between the simulated and the actual velocity and flowrate is not significant. Also, "flushing through a drain valve" is very effective to flushing a long-length pipe section because of its efficiency to obtain the flushing velocity. However, the number and location of a drain valve is limited compared to a hydrant so that "flushing through a drain valve" has a limited application in the field. For this reason, the engineer should consider various field conditions to come up with a proper flushing plan.

A stratified random sampling design for paddy fields: Optimized stratification and sample allocation for effective spatial modeling and mapping of the impact of climate changes on agricultural system in Korea (농지 공간격자 자료의 층화랜덤샘플링: 농업시스템 기후변화 영향 공간모델링을 위한 국내 농지 최적 층화 및 샘플 수 최적화 연구)

  • Minyoung Lee;Yongeun Kim;Jinsol Hong;Kijong Cho
    • Korean Journal of Environmental Biology
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    • v.39 no.4
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    • pp.526-535
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    • 2021
  • Spatial sampling design plays an important role in GIS-based modeling studies because it increases modeling efficiency while reducing the cost of sampling. In the field of agricultural systems, research demand for high-resolution spatial databased modeling to predict and evaluate climate change impacts is growing rapidly. Accordingly, the need and importance of spatial sampling design are increasing. The purpose of this study was to design spatial sampling of paddy fields (11,386 grids with 1 km spatial resolution) in Korea for use in agricultural spatial modeling. A stratified random sampling design was developed and applied in 2030s, 2050s, and 2080s under two RCP scenarios of 4.5 and 8.5. Twenty-five weather and four soil characteristics were used as stratification variables. Stratification and sample allocation were optimized to ensure minimum sample size under given precision constraints for 16 target variables such as crop yield, greenhouse gas emission, and pest distribution. Precision and accuracy of the sampling were evaluated through sampling simulations based on coefficient of variation (CV) and relative bias, respectively. As a result, the paddy field could be optimized in the range of 5 to 21 strata and 46 to 69 samples. Evaluation results showed that target variables were within precision constraints (CV<0.05 except for crop yield) with low bias values (below 3%). These results can contribute to reducing sampling cost and computation time while having high predictive power. It is expected to be widely used as a representative sample grid in various agriculture spatial modeling studies.

Behavior Analysis of Concrete Structure under Blast Loading : (I) Experiment Procedures (폭발하중을 받는 콘크리트 구조물의 실험적 거동분석 : (I) 실험수행절차)

  • Yi, Na Hyun;Kim, Sung Bae;Kim, Jang-Ho Jay;Choi, Jong Kwon
    • KSCE Journal of Civil and Environmental Engineering Research
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    • v.29 no.5A
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    • pp.557-564
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    • 2009
  • In recent years, there have been numerous explosion-related accidents due to military and terrorist activities. Such incidents caused not only damages to structures but also human casualties, especially in urban areas. To protect structures and save human lives against explosion accidents, better understanding of the explosion effect on structures is needed. In an explosion, the blast overpressure is applied to concrete structures as an impulsive load of extremely short duration with very high pressure and heat. Generally, concrete is known to have a relatively high blast resistance compared to other construction materials. However, information and test results related to the blast experiment of internal and external have been limited due to military and national security reasons. Therefore, in this paper, to evaluate blast effect on reinforced have concrete structure and its protective performance, blast tests are carried out with $1.0m{\times}1.0m{\times}150mm$ reinforce concrete slab structure at the Agency for Defence Development. The standoff blast distance is 1.5 m and the preliminary tests consists with TNT 9 lbs and TNT 35 lbs and the main tests used ANFO 35 lbs. It is the first ever blast experiment for nonmilitary purposes domestically. In this paper, based on the basic experiment procedure and measurement details for acquiring structural behavior data, the blast experimental measurement system and procedure are established details. The procedure of blast experiments are based on the established measurement system which consists of sensor, signal conditioner, DAQ system, software. It can be used as basic research references for related research areas, which include protective design and effective behavior measurements of structure under blast loading.

A Study on Problems and Improvement of Home-help Services of Long-term Care Insurance (노인장기요양보험 재가서비스의 문제점과 개선방안)

  • Lee, Jun Woo;Jin, Hee
    • 한국노년학
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    • v.29 no.1
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    • pp.149-175
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    • 2009
  • The purpose of this research is to analyze the overall problems at the moment of October 2008, and then to find the improvements of home-help services of the Long-Term Care Insurance(LTCI), which has been revealed many problems since it was released in July 2008. The research uses the literature survey which analyzes 2nd-hand materials studied by other people already, and survey research was executed from active social workers in the area of LTCI. Based on the policy analysis framework of Gilbert and Specht, all the data are analyzed in the scopes of client·benefit(service)·finance·transferring system. This research has found the problems in each scope of home-help services of the LTCI. Firstly, the client system has some problems in mismatching between registered and service clients, estimating client number, and judging service levels. Secondly, the service system reveals deficiency in professionality of social workers, service quality lowering by loose qualification criteria on workers, non-reasonable limitation of service time available, and the same fare system applied to visiting-help service in spite of different levels. Thirdly, in financing system, clients need to pay additional money to get extra services such as meal, hair cutting, bathing etc., due to government financial support stopped, some organizations have to reduce services and replace full-time workers to part-time ones, which makes the service quality worse. Lastly, in the transferring system, the management system for service quality is not well prepared. There are too much competion because of allowing too many home-help service organizations and care worker academies. The suggestions that this research has found to improve the policy are as follows. ① It is desirable to make the registered clients the service ones as many as possible in the long term perspective. ② The LTCI organization requires more workers and higher professionality. ③ Many elderly people who are not eligible now require connection system to be more served. ④ Management system and service manual for care worker are to be developed. ⑤ Laws related to the service contents and process should be modified, the proportion of client charge needs to adjust. ⑥ Home-help service organization licensed by the LTCI needs to be financially supported publicly. ⑦ Monitoring system to home-help service organization needs to be strengthened. ⑧ Evaluation tools to home-help service organization and workers is required. ⑨ Specification to open the home-help service organization needs to be more strict.