• Title/Summary/Keyword: 시간 분해 측정

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Effect of Pepsin-HC$\ell$ Concentration and Digestion Time on the Protein Digestibility of the Cattle Skin Meal (Pepsin농도와 소화시간이 우피분의 단백질 소화율에 미치는 영향)

  • 김대진
    • Korean Journal of Poultry Science
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    • v.11 no.1
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    • pp.13-17
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    • 1984
  • Protein digestibitlities of hydrolyzed cattle skin meals were examined at a constant pepsin-MC$\ell$ concentration (0.2%) for varying lengths of incubation time (from 4 to 20 hours) and at varying concentrations of pepsin-HC$\ell$ (from 0.0125 to 0.2%) for 16 hours at 45$^{\circ}C$. The results are summarized as follows: 1. Protein digestibilities of hydrolyzed cattle skin meals in 0.2% pepsin-HCl were 66.31%, 80.69%, 83.72%, 84.65% and 81.45% for 4, 8, 12, 16 and 20 hours incubation, respectively. Protein digestibilities were maintained above 80% for 8-hour incubation and were increasing incubation time. 2. Protein digestibilities of hydrolyzed cattle skin meal incubated for 16 hours at 0.2%, 0.1%, 0.05%, 0.025% and 0.0125% pepsin-HC$\ell$ solution were 85.10%, 82.08%, 76.18%, 74.67% and 64.82%, respectively. Protein digestibilities were decreased with decreasing pepsin concentration.

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Studies on Chemical Strutures and Adhesion Performance of pMDI Adhesives Modified by Ozonized Soybean Oil with Different Mixing Ratios (오존산화 콩기름의 구조분석 및 이를 이용한 변성 pMDI 접착제의 중량비에 따른 접착력 변화)

  • You, Young Sam;Lee, Hyun Jong;Lee, Taek Jun;Park, Heon
    • Journal of the Korean Wood Science and Technology
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    • v.37 no.1
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    • pp.56-64
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    • 2009
  • The purpose of this study was to investigate and develop an eco-friendly wood adhesive based on vegetable oil (especially soybean oil), the renewable and sustainable natural resources, using ozonification technology for the chemical structure modification. The soybean oil (SBO) was reacted with $O_3$ at the rate of 7.13 g/h for different times, 15 minutes, 30 minutes, 60 minutes, and 120 minutes. The investigation of the modified chemical structure of the ozonized SBOs were conducted using FT-IR, $^1H$-NMR, MALDI-TOF MS, and GC/MS. As ozonification time increased, the peak of the unsaturated double bonds was disappeared and aldehyde or carboxyl peak appeared because ozonification broke the oil into small molecules. The plywoods were made at $110^{\circ}C$ with 30 seconds/mm hot-press time using the different ozonized SBO/pMDI adhesives and were tested for the dry, wet, cyclic boil test according to the Korea Industrial Standard F3101 Ordinary plywood. The bond strengths gradually increased with increasing ozonification time. The weight ratio 1:1 (ozonized SBO/pMDI), all strengths in 15, 30 and 60 minuets, exceeded constantly the dry, wet, cyclic boiling standard requirement. The range of ozonification time and weight ratio can fulfil1 the requirment of the wet test standard were 30~60 minutes and more than 0.5 pMDI. From the comprehensive view on the results of above experiments, it could be confirmed through experiments that ozonized SBO/pMDI has characteristics of effective reactivity and wet stability showed as an excellent candidate of wood adhesive applications.

Change of isometric contractile force and muscle activity applying ice and hot according to the time on biceps brachii muscle (온열 및 한랭의 적용시간에 따른 상완이두근의 등척성 수축력과 근활성도 변화)

  • Lee, Joon-Hee;Jeon, Jae-Keun
    • Journal of Digital Convergence
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    • v.10 no.11
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    • pp.459-465
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    • 2012
  • This study has investigated the effect of isometric contractile force and muscle activity applying ice and hot according to the time from the biceps brachii muscle. In this study, 20 university students participants without musculoskeletal and neurological disorders. By applying a hot pack 5 min, 10 min, 20 min and 30 min respectively and ice pack for 5 min, 10 min, and 20 min respectively. After that measurement are skin temperature, contractile force and muscle activity. Skin temperature of the hot and cold 5 min applied that rapidly changing. Increasing the time it takes to apply a variance has been reduced(p<.001). Isometric contractile force was not statistically significant but highest when applying the hot pack 5 minutes and lowest when applying the hot pack 30 minutes(p<.001). Muscle activity and median frequency was highest when applying the hot pack 5 minutes. To analyze the above results, it was found that isometric contractile force and muscle activity changed according to the applying time. These result lead us to the conclusion that this study will be more evidence for changes in muscle contraction to apply hot pack and ice pack on clinic.

ECG Filtering using Empirical Mode Decomposition Method (EMD 방법을 이용한 ECG 신호 필터링)

  • Lee, Geum-Boon;Cho, Beom-Joon
    • Journal of the Korea Institute of Information and Communication Engineering
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    • v.13 no.12
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    • pp.2671-2676
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    • 2009
  • Empirical mode decomposition (EMD) is new time-frequency analysis method to decompose the signal adaptively and efficiently. The key idea of EMD is to decompose the signal into a set of functions defined by the signal itself, named Intrinsic Mode Functions (IMFs), which preserve the inherent properties of the original signal. Since the decomposition is based on the local time scale of the signal, it is not only applicable to nonlinear and non-stationary processes but also useful in biomedical signals like electrocardiogram (ECG). Traditional low-pass filter uses fourier transform to analysis signal in frequency domain, but EMD is filtered to maintain signal properties in time domain. This paper performed signal decomposition and filtering for noisy ECGs using EMD method. The proposed method is presented and compared with traditional low-pass filter by two performance indices. Our results show effectiveness for enhancement of the noisy ECG waveforms.

Preparation Condition of Chitooligosaccharide by Cellulase using Response Surface Methodology (반응표면 분석법을 이용하여 Cellulase에 의한 키토산올리고당의 제조 조건 설정)

  • Joo Dong Sik;Lee Jung Suck;Kim Ok Seon;Cho Soon Yeoung
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.35 no.6
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    • pp.696-701
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    • 2002
  • Optimal conditions for preparing of chitooligosaccharides from chitosan with cellulase was researched by response surface methodo-logy, Penicillium funiculosum derived cellulase was most effective for chitooligosaccharides production as the point of hydrolyzing activity and commercial utility. The result which measures the change of degrading ratio at time course, 10 hr reaction showed a exponential increase and after that time degrading ratio was not changed. The optimal conditions determined by response surface methodology with central composite design of total 26 species were $0.5\%$ of chitosan, 143 U enzyme, 49$^{\circ}C$ of reaction temperature, 13.2 hr of reaction time and pH 3.8. Major chitooligosaccharides produced from chitiosan on optimal conditions were dimer and trimer.

Cardiac Function of Asphyxiated Rat Hearts (질식사한 흰 쥐 심장의 기능평가)

  • 조준용;허동명
    • Journal of Chest Surgery
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    • v.29 no.3
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    • pp.255-262
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    • 1996
  • The donor pool for heart transplants is severely limited and there is still a legal problem of brain death. This study assessed the function of hearts "absolute anoxic" for ten minutes after asphyxia by perfusing the hearts on a Langendorfr apparatus for 45 minutes with Krebs-Henseleit buffier at 37 t at 80 cm H2O. Forty isolated rat hearts were divided into four groups. Ten control hearts (group 1) were perfused on the circuit without intervening ischemia. Ten hearts (group 2) were harvested, quickly flushed with 5cc of cold University of Wisconsin solution, and stored in the same cold solution for 4 hours. Ten hearts (group 3) were excised, quickly flushed with 5 u of cold Stanford cardioplegic solution and stored in cold saline solution for 4 hours. Ten asphyxiated hearts (group 4) had warm ischemia for ten minutes and were perfused with 5u of cold Stanford cardioplegia containing 7,500 units of urokinase to dissolve intravascular clots, and stored in cold saline solution for 1.5 hours. Time of spontaneous defibrillation (TSD) after perfusion was significantly longer in group 2, group 3 and group 4 than in group 1. TSD in group 3 and group 4 was significantly longer in comparison to that of group 2. Left ventricular developed pressure(LVDP) at 15 minutes was significantly lower in group 3 and group 4 than in group 1 and group 2. In group 4, LVDP at 30 minutes and 45 minutes was significantly lower compared with that in group 1 . In conclusion, asphyxiated rat hear;ts which had absolute anoxia for 10 minutes after as hyxia showed relatively satisfactory cardiac function. function.

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Development of High-Sensitivity and Entry-Level Nuclide Analysis Module (고감도 보급형 핵종 분석 모듈 개발)

  • Oh, Seung-Jin;Lee, Joo-Hyun;Lee, Seung-Ho
    • Journal of IKEEE
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    • v.26 no.3
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    • pp.515-519
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    • 2022
  • In this paper, we propose the development of a high-sensitivity entry-level nuclide analysis module. The proposed measurement sensor module consists of an electronic driving circuit for nuclide analysis resolution, prototype production with nuclide analysis function, and GUI development applied to prototypes. The electronic part driving circuit for nuclide analysis resolution is divided into nuclide analysis resolution process by the electronic part driving circuit block diagram, MCU circuit design used for radiation measurement, and PC program design for Spectrum acquisition. Prototyping with nuclide analysis function is made by adding a 128×128 pixel OLED display, three buttons for operation, a Li-ion battery, and a USB-C type port for charging the battery. The GUI development department applied to the prototype develops the screen composition such as the current time, elapsed measurement time, total count, and nuclide Spectrum. To evaluate the performance of the proposed measurement sensor module, an expert witness test was conducted. As a result of the test, it was confirmed that the calculated result by applying the resolution formula to the Spectrum (FWHM@662keV) obtained using the Cs-137 standard source in the nuclide analysis device had a resolution of 17.77%. Therefore, it was confirmed that the nuclide analysis resolution method proposed in this paper produces improved performance while being cheaper than the existing commercial nuclide analysis module.

The Novel Assay Method for Thrombin by Weighing Fibrin Clot (피브린의 무게측정에 의한 새로운 트롬빈활성측정법)

  • Park, Inshik;Kim, Gi-Nahm
    • Journal of Life Science
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    • v.14 no.3
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    • pp.441-444
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    • 2004
  • This study was performed to establish a simple and rapid method for measuring thrombin activity based on weight of fibrin clot formed. The new method was based on the weight measurement of fibrin clot after enzymatic reaction of thrombin with fibrinogen. The fibrin formation depended upon the activities of thrombin used, temperature, incubation time, and centrifugation time. The fibrin formation was increased proportionally up to 1.0 unit/ml of thrombin activity, 4.0mg/ml of fibrinogen concentration, and 5 min of incubation time at 37$^{\circ}C$. The fibrin clot formed was stable by centrifugation at 3,000$\times$g for 5min. This simple assay based on weight of fibrin after centrifugation would be useful for identifying natural food anticoagulants by inhibiting thrombin.

Degradation of BTX by Aerobic Microbial Consortium (호기성 미생물 컨소시엄에 의한 BTX의 분해)

  • 문종혜;김종우;박진수;오광중;김동욱
    • KSBB Journal
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    • v.16 no.1
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    • pp.61-65
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    • 2001
  • In this study, a BTX degrading microbial consortium was obtained from the activated sludges of a BTX releasing sewage water and city sewage water treatment plant. The MY microbial consortium was developed for benzene and toluene degradation, whereas the MA microbial consortium was developed for xylene isomers. The major microorganism of the MA consortium was identified as Rhodococcus ruber DSM 43338T, whereas that of the MY consortium was Rhodococcus sp. In terms of the degradation of a single component, the removal rate of benzene was fastest and decreased in order; toluene, o-xylene, p-xylene and m-xylene. For degradation of mixed BTX, most BTX were degraded within 108 hours and the degradation rate showed either stimulatory or inhibitory effects depending on the composition. MA and MY microbial consortium obtained in this study may be used effectively to remove BTX biologically.

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Effect of Roasting Conditions on the Antioxidant Activities of Cassia tora L. (볶음 공정이 결명자의 항산화 활성에 미치는 영향)

  • Lee, Myung-Hye;Cho, Jin-Ho;Kim, Bum-Keun
    • Korean Journal of Food Science and Technology
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    • v.45 no.5
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    • pp.657-660
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    • 2013
  • The effects of roasting temperature and time on the antioxidant activities of Cassia tora L. were investigated. In comparison with raw seeds (7.15 mg TAE/g), roasted seeds contained a significantly higher total polyphenol content (p<0.05). However, seeds roasted at a higher temperature ($250^{\circ}C$) for 10 min showed a significantly lower total polyphenol content (2.30 mg TAE/g; p<0.05). The electron donating abilities of Cassia tora L. seeds increased with an increase in roasting time; further, seeds roasted for 5 min at lower temperatures showed higher electron donating abilities (80.61% at $175^{\circ}C$; 80.75% at $200^{\circ}C$) than did seeds roasted for 5 min at higher temperatures (76.26% at $225^{\circ}C$; 77.35% at $250^{\circ}C$). Seeds roasted at lower temperatures showed adequate L values, regardless of roasting time; by contrast, seeds roasted for 10 min at higher temperatures, showed markedly lower L values. Our results indicate that roasting temperature and time must be controlled to produce high-quality Cassia tora L. products.