Journal of Chest Surgery

The Korean Society for Thoracic and Cardiovascular Surgery (대한심장혈관흉부외과학회)
권호 표지

Cardiac Function of Asphyxiated Rat Hearts

질식사한 흰 쥐 심장의 기능평가

  • 조준용 (경북대학교 의과대학 흉부외과학교실) ;
  • 허동명 (경북대학교 의과대학 흉부외과학교실)
  • Published : 1996.03.01
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Abstract

The donor pool for heart transplants is severely limited and there is still a legal problem of brain death. This study assessed the function of hearts "absolute anoxic" for ten minutes after asphyxia by perfusing the hearts on a Langendorfr apparatus for 45 minutes with Krebs-Henseleit buffier at 37 t at 80 cm H2O. Forty isolated rat hearts were divided into four groups. Ten control hearts (group 1) were perfused on the circuit without intervening ischemia. Ten hearts (group 2) were harvested, quickly flushed with 5cc of cold University of Wisconsin solution, and stored in the same cold solution for 4 hours. Ten hearts (group 3) were excised, quickly flushed with 5 u of cold Stanford cardioplegic solution and stored in cold saline solution for 4 hours. Ten asphyxiated hearts (group 4) had warm ischemia for ten minutes and were perfused with 5u of cold Stanford cardioplegia containing 7,500 units of urokinase to dissolve intravascular clots, and stored in cold saline solution for 1.5 hours. Time of spontaneous defibrillation (TSD) after perfusion was significantly longer in group 2, group 3 and group 4 than in group 1. TSD in group 3 and group 4 was significantly longer in comparison to that of group 2. Left ventricular developed pressure(LVDP) at 15 minutes was significantly lower in group 3 and group 4 than in group 1 and group 2. In group 4, LVDP at 30 minutes and 45 minutes was significantly lower compared with that in group 1 . In conclusion, asphyxiated rat hear;ts which had absolute anoxia for 10 minutes after as hyxia showed relatively satisfactory cardiac function. function.

본 연구는 횐 쥐의 적출심장을 이용한 질식사심장의 기능평가로서,기도압박에 의한 질식으로 심정 지가 일어난 후, 10분 지나서 심장을 적출하고 체외관류장치에 연결하여, 80cmH20높이에서 37C의 Krebs-Henseleit용액을 적출 심장에 관류시켜, 그 기능을 평가하여 다음과 같은 결과를 얻었다. Sprague-Dawley계 흰 쥐를, 제 1군(10마리)은 대조군, 제2군(10마리)은 위스콘신대학용액 저장군, 제3 군(10마리)은 스탠포드심정 지용액-생 리식 염수 저장군, 제4군(10마리)은 질식사군으로 분류하여 실험을 시행하였다. 자연 제세동시간은, 모든 군에서 대조군보다 길었으며, 제2군에 비 해서 제3군과 제4군의 제 세동시간도 길었다(p < 0.05). 좌심실내압은, 관류 15분, 30분, 45분에서, 대조군 및 제2군에 비하여 제3 군이 모두 낮았으며,제4군은 관류 15분에, 대조군과 제2군의 15분에 비하여 낮았고, 관류 30분과45분 에는 대조군에 비하여 낮았다(p < 0.05). 관류 15분, 30분, 45분에 측정한 심박동수와 좌심실내압을 곱한 간은, 제3군에서 대조군과 제2군에 비하여 모두 낮았으며, 제4군에서는 대조군에 비하여 모두 낮았고, 제4군의 15분값은 제2군의 15분값에 舟漫\ulcorner\ulcorner낮았다(p<0.05).관류45분에 측정한심근의 수축예비 력은, 대조군과 비교하여 제3군과 제4군이 모두 낮았으며, 제3군은 제2군에 비하여도 낮았다(p < 0.05). 이상의 결과로 보아,질식사로 사망한 조기 적출심장의 기능은, 허혈손상이 없는 대조군과 위스콘신 대학용액에 저장한 심장의 기능에 비하여 그 성적이 불리하나,스탠포드심정지용액-생리식염수 저장군 의 심기능 평가 결과와는 유의한 차이가 없어, 공여심장으로서의 심기능은 비교적 만족할 만한 것으로 볼 수 있다.

Keywords

References

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