• The Journal of Korean Society of Virology
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• v.26 no.1
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• pp.23-30
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• 1996

대략 36,000 base pairs (bp)의 두 가닥짜리 DNA를 지놈으로 가진 사람 아데노바이러스 (Ad)는 DNA 상동성(相同性) 및 생물학적/생화학적 성격이 특이한 49개의 혈청형이 알려져 있는데, 이들 대부분의 Ad가 영유아군 및 면역능이 저하된 성인에서 치사적 결과를 초래할 수 있다. Ad의 세포향성(向性)(tropism)은 매우 다양하여 종류에 따라 상기도 감염, 각결막염, 영유아 장염등을 유발하는데 최근 Ad의 다양한 병원성에 대한 원인을 분자생물학적 수준에서 규명하려는 노력의 일환으로 지역에 따라 주되게 출현하는 Ad형 규명이 활발히 이루어지고 있다. Ad 동정/확인은 표면을 이루고 있는 group 공통항원인 hexon 단백질을 탐지하는 효소면역 측정법 (EIA)에 의하며, Ad형별은 Ad fiber의 세포독성 중화시험에의 한다. 그러나, 세포독성 중화시험이 엄청난 노동력 및 시간을 요구하면서도 민감도/특이도가 만족스럽지 못하여 이를 개선하기 위하여 검체 또는 세포배양에서 Ad DNA를 추출하여 제한효소 절단형태를 비교하는 방법이 개발되었는데 이는 세포배양에 잘 자라지 않는 바이러스주의 형별뿐만 아니라 지역 분리주들의 지놈 변형주를 관찰하는 분자생물학적/분자역학적 연구에도 도움이 되고 있다. 국내에는 Ad와 관련된 소아장염의 빈도가 rotavirus에 의한 것 다음으로 빈번한데도 Ad40/41외에 주되게 출현하는 장내 Ad형들이 전혀 규명된 바 없고, 한국형 Ad들의 지놈형태가 전혀 보고된 바가 없다. 또한 세계적으로 Ad형별 조사지역이 늘어감에 따라 유아장염과 연관된 Ad 역시 Ad40, 41이 외의 형들이 Ad40, 41을 능가하는 것으로 보고되고 있는 지역도 있으나 국내에서는 Ad40, 41이외의 형들은 그 역학적 중요도가 전혀 알려져 있지 않다. 이로서 본 연구의 목적은 Ad주들에 특이 중화항체를 이용한 세포독성 중화시험과 Ad DNA 절단법을 적용하여 한국형 장내 Ad주들의 형별을 처음으로 시도함과 동시에 1989-1991사이 출현한 Ad들의 유전적 변형을 관찰하려는 것이었다. 두 방법 모두 사용하였을 때 주되게 출현하는 장내 Ad형들은 Ad4l, Ad2, Ad7, Ad5, 및 Ad40이었다. Ad40/41-양성 검체를 제외한 Ad hexon-EIA양성들의 77.5%를 형별 할 수 있었던 Ad DNA의 제한효소 절단방법은 형들간의 교차중화로 특이성이 낮았던 중화방법 (47.5%)보다 매우 효율적이어서 두 가지 방법을 함께 적응하였을 때는 40주중의 81.5%인 35주를 형별 할 수 있었다. 또한Ad DNA 제한 효소 절단방법은 Ad7 변이주 (Ad7b)도 탐지 할 수 있었다.

• Korean Journal of Soil Science and Fertilizer
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• v.31 no.1
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• pp.39-45
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• 1998

As a basic research for developing a sludge application technique in forestry, this study was conducted to investigage a reasonable amount and period of sludge to be applied onto forest nursery of Acer palmatum Thunb. The paper mill sludge was used for the study, and ammonium nitrate was treated to some of them to reduce the C/N ratio lower than 40. The maximum amount of applied sludge was 7ton/ha/year, and they were spread on spring and on late summer. The sludge application did not stimulate early growth of the trees during a year. Since there was no significant changes in soil chemical properties, the amount of applied sludge was thought to be too little to result in a significant role for tree growth. However, the soil water showed significant increase of Cu when the activated sludges were applied during late summer. Thus, the time of application need to be determined cautiously with considering the risk of ground water contamination. In conclusion, if we set a goal from the view point of long-term concept such as forest productivity rather than to get a short-term product, the uncomposted sludge in small amount during growing season might be considered to be applied on forest nursery.

• Sleep Medicine and Psychophysiology
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• v.10 no.1
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• pp.61-66
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• 2003

Objectives: The purpose of this study is to investigate the relationship between the extent of prolongation in P300 latency by sleep deprivation and personality variables. Methods: Eighteen healthy male college students participated in this study. Subjects remained awake for 37 hours under continuous surveillance. In the morning and evening of two consecutive study days, P300 was checked four times. MMPI and STAI-T were checked in the morning of the first day. The 18 subjects were divided into two groups according to their extent of P300 prolongation by sleep deprivation: Group A consisted of short P300 latency prolonged subjects, and group B of long latency prolonged subjects. The MMPI profiles and STAI-T scores of these two groups were compared. Results: Group B showed significantly higher scores in the Mf subscale (t=-2.16, df=16, p=0.046) and Pa subscale (t=-2.61, df=16, p=0.019) than group A. Group B also showed higher F subscale scores at a marginally significant level (t=-2.11, df=16, p=0.052). Conclusion: These results suggest that subjects with higher scores in F, Mf, and Pa subscales tend to have delayed cognitive process and decreased efficiency of mental process by sleep deprivation. It can be hypothesized that individuals who are sensitive, passive, dependent, and easily projecting are susceptible to the deterioration of cognitive function by total sleep deprivation.

• Water for future
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• v.16 no.4
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• pp.245-252
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• 1983

Recently river water pollution in Korea is given rise to serious problem in aspect of crop production, drinking well, water contamination and etc. Under these urgent situations, it is prime importance to protect water resources from pollutants. An environmental isotope survey of the groundwater form the shallow alluvial and the underlying crystalline rock aquifer of the Han River Basin has been undertaken, Analysis of the data has I) confirmed the hypothesis that the groundwater from the metropolitan area is recharged from the river whereas that form the non-urbanized region of the Basin is replenished by the infiltrating precipitation; ii) shown that crystalline rock aquifers are recharged by the ground water form the overlying alluvium. Old groundwater is a group of wells with tritium values in the range of 0 to 2 TU. These low values indicate that the water sampled was recharged much ealier, at least a few decades, than the other groundwater samples of higher tritium content. The low values in this region may, in fact, reflect the effect of the impermeable clay layers which impede infilteration from the surface. Stable isotope evidence confirmed that a recharge in the karst area occurs at a significantly greater elevation than that to the alluvial aquifer. An analysis of the tritium level collected over an annual cycle suggests that the residence time of groundwater is probably not more than a few months. There does not appear to be any correlation between the trace level of Zn, Mn and Pb in the groundwater and the mechanism of the recharge.

• Journal of Life Science
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• v.14 no.5
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• pp.800-808
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• 2004

Resveratrol, a phytoalexin found at high levels in grapes and in grape products such as red wine, has been reported to possess a wide range of biological and pharmacological activities including antioxident, anti-inflammatory, anti-mutagenic, and anti-carcinogenic effects. According to recent studies, this compound is an effective inhibitor of cell growth in general, triggers partial arrest of the cell cycle and induce apoptosis. In this study, the anti-proliferative effects of resveratrol in A549 human lung carcinoma cells were investigated. It is shown that resveratrol induced the growth inhibition in a time-dependent manner and morphological changes of A549 cells, which were associated with induction of S phase arrest of the cell cycle and apoptotic cell death. The Bcl-$X_L$levels were markedly down-regulated in resveratrol treated cells, however, Bax and Bcl-2 were remained unchanged. Resveratrol treatment induced the proteolytic degradation of Sp-l and proliferating cell nuclear antigen protein, and inhibited the expression of $\beta$-catenin protein. Resveratrol treatment also induced a marked up-regulation of cyclin-dependent kinase (Cdk) inhibitor p21 and inhibited the kinase activities of Cdk2 and Cdk4. In addition, resveratrol treatment inhibited the levels of cyclooxygenase (COX)-2 mRNA and protein, and the release of prostagladin E2 without alteration of COX-1 expression. Taken together, these findings suggest that resveratrol may be a potential chemotherapeutic agent for the control of human lung carcinorma cells.

• Journal of the Korea Society of Computer and Information
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• v.16 no.1
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• pp.19-29
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• 2011

As people have heightened attention to blogs that are individual media, a variety rank algorithms was proposed for the blog search. These algorithms was modified for structural features of blogs that differ from typical web sites, and measured blogs' reputations or popularities based on the interaction results like links, comments or trackbacks and reflected in the search system. But actual blog search systems use not only blog-ranks but also search words, a time factor and so on. Nevertheless, those might not produce desirable results. In this paper, we suggest a topic-rank technique, which can find blogs that have significant degrees of association with topics. This technique is a method which ranks the relations between blogs and indexed words of blog posts as well as the topics representing blog posts. The blog rankings of correlations with search words are can be effectively computed in the blog retrieval by the proposed technique. After comparing precisions and coverage ratios of our blog retrieval system which applis our proposed topic-rank technique, we know that the performance of the blog retrieval system using topic-rank technique is more effective than others.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.20 no.2
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• pp.368-377
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• 2019

The purpose of this study is to examine the present operation process and to suggest the points of improvement so that vocational education and training institutions can be activated more effectively. The subjects of this study were 250 college students who completed or attended the vocational education and training course in Seoul and Gyeonggi-do, and collected 230 data, excluding inappropriate responses. Data analysis was performed by using the statistical package program SPSS Statistics (Ver. 24: IBM, USA). In this study, frequency analysis was conducted to examine the characteristics of data, and crossover analysis was conducted mainly to investigate the correlation between categorical data. The results of this study are as follows: First, it provides information about vocational education and training course to all general high school students at the beginning of the first year of high school, and then, from the first semester of the second grade, do. Second, it is to open four major courses in beauty education at the same time. This makes it possible to acquire qualifications according to four beauty courses during the training period at the training institute rather than the private education institute where private education expenses are incurred. By training all major courses in skin, hair, make-up, and nail art, you will be given the opportunity to carefully determine the major course that fits your aptitude. Based on the results of this research, we propose a plan to improve the vocational education system for vocational high school students who are majoring in cosmetology.

• Journal of Korean Historical Folklife
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• no.44
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• pp.7-37
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• 2014

The people of Silla was described as the descendants of Hwagwan(official of fire) on the tombstone of King Munmu(文武王), and Gim Yusin(金庾信) was described as the descendants of Shaohao Jintian(少昊 金天) and Huangdi Xuanyuan(黃帝 軒轅) on his tombstone. It says that the royal households of Silla and Gaya had common ancestor. Hwagwan was the official who took charge of fire management and the ritual for Antares(${\alpha}$ Sco) in ancient China. Hed founded State Ra(羅國). The name of Silla(新羅) means new State Ra, so he could become the ancestor of the people of Silla. He was the son of Zuanxu Gaoyang. State Gaya(加耶), the fatherland of Gim Yusin had been called Geumgwan-gug(金官國) which means the state of official of metal. Geumgwan was the son of Shaohao Jintian. Silla was the state of Hwagwan and the Gaya was the state of Geumgwan. Hwagwan, the founder of the royal household of Silla was the son of Zuanxu and Geumgwan, the founder of the royal household of Gaya was the son of Shaohao. Zuanxu and Shaohao was the descendants of Hwangdi, so Hwangdi was the common ancestor of Silla and Gaya. Finally Hwangdi became the same ancestor of Gim Yusin and King Muyeol(武烈王) who was the father of King Munmu. The tombstone of King Munmu and Gim Yusin manifests the union of the blood of Gim Yusin and King Muyeol. But it was not the fact but the rhetorical fiction.

• Journal of Plant Biotechnology
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• v.49 no.4
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• pp.307-315
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• 2022

Artemisinin is a secondary metabolite of Artemisia annua that shows potent anti-malarial, anti-bacterial, antiviral, and anti-tumor effects. The supply of artemisinin depends on its content in Artemisia annua, in which various environmental factors can affect the plant's biosynthetic yield. In this study, the effects of different light-emitting diode (LED)-irradiation conditions were tested to optimize the germination and growth of Artemisia annua for the enhanced production of artemisinin. Specifically, the ratio between the red and blue lights in the irradiating LED was varied for investigation as follows: [Red : Blue] = [6 : 4], [7 : 3], and [8 : 2]. Furthermore, additional stress factors like UV-B-irradiation (1,395 ㎼/cm²), low temperature (4℃), and dehydration were also explored to induce hormetic expressions of ADS, CYP, and ALDH1, which are essential genes for the biosynthesis of artemisinin. Quantitative polymerase chain reaction (qPCR) was used to analyze the expression levels of the respective genes and their correlation with the specified conditions. [8 : 2] LED-irradiation was the most optimal among the tested conditions for the cultivation of Artemisia annua in terms of both fresh and dry weights post-harvest. For the production of artemisinin, however, [7 : 3] LED-irradiation with dehydration for six hours pre-harvest was the most optimal condition by inducing around twofold enhancement in the biosynthetic yield of artemisinin. As expected, a correlation was observed between the expression levels of the genes and the contents of artemisinin accumulated.

• The Journal of Engineering Geology
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• v.34 no.3
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• pp.429-445
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• 2024

The Journal of Engineering Geology (JEG), a leading academic journal in the field of engineering geology in South Korea, was first published in 1991 and has since been publishing academic papers and research findings. While several literature reviews have been undertaken on specific research areas in recent decades, comprehensive reviews focusing on JEG have been relatively limited. To address this gap, this study applied the latent Dirichlet allocation (LDA) model to analyze the main research topics and trends, and undertook network analysis to identify relationships between topics over different periods. Results for the LDA indicate seven key research topics categorized into three trends: Classic, Emerging and Stable topics. Classic topics include 'Geophysics' and 'Structural geology', which were major subjects in the early years, with their focus shifting to other areas over time. Emerging topics such as 'Hydrogeology' and 'Geohazards' have gained prominence in recent years. Stable topics including 'Geotechnical structures', 'Geomechanics', and 'Environmental geology' have maintained consistent research interest. Network analysis revealed that Structural geology was the central topic prior to 2008, while Geotechnical structures became the focal point of research after 2008, with a shift in research focus. The results of this study contribute to our understanding of research trends and the development of JEG, providing insights for the setting of future research directions.