• Proceedings of the Korean Society of Embryo Transfer Conference
• /
• 2002.11a
• /
• pp.106-106
• /
• 2002

미토콘드리아는 세포내의 에너지대사에서 중요한 역할을 수행하는 세포내 소기관이며, 자체의 유전물질이 모계를 통해 유전되는 특징을 가지고 있다. 포유류 초기 배아의 발생과정에서 미토콘드리아의 역할과 기능에 대한 연구는 미진한 상태이다. 본 연구에서는 생쥐 초기 배아의 발생과정에서 관찰할 수 있는 미토콘드리아 미세구조의 변화 양상을 살펴보고, 이와 초기 배아의 발생 능력과의 관련성을 밝혀보고자 하였다. 과배란 유도된 ICR 생쥐로부터 배란된 난자와 2-세포기 배아를 수획하여 76 배양액으로 포배기까지 체외배양하면서, 각각의 발생단계에 따라 시료를 수획하였다. 미토콘드리아 미세구조의 변화는 일반적인 투과전자현미경방법(TEM)을 이용하여 관찰하였다. 미토콘드리아의 미세구조는 배란 난자에서 4-세포기 배아까지는 구형이고 크리스타가 발달하지 않은 원시형태였지만, 포배기로 발달함에 따라 크리스타가 발달된 막대형의 전형적인 미토콘드리아로 분화됨이 관찰되었다. 체외배양 중에 발생이 지연되거나 정지된 배아에서 관찰한 미토콘드리아의 미세구조는 공포화 (vacuolization), 크리스타 발달 지연, 손상된 미토콘드리아의 세포막 등과 같은 비정상적인 변형을 관찰할 수 있었다. 또한, 극체에 존재하는 미토콘드리아의 미세구조는 정상적인 핵내의 유전자와의 상호작용이 없어 미분화 상태로 포배기까지 유지되는 것을 관찰하였다. 이상의 결과를 통해 미토콘드리아의 정상적인 분화 과정이 초기 배아의 발생능력과 관련되어 있음을 알 수 있었으며, 포유류 초기 배아의 체외배양시스템을 개선하는데 미토콘드리아 미세구조의 관찰과 변화에 대한 고려가 있어야 될 것으로 생각된다. buffer A 용액으로 세척하여 유리 petri dish의 바닥에 부착된 macrophage만을 cell scraper로 분리하였다. 분리한 macrophage는 0.5-1 $\times$ $10^{6}$ cells/$m\ell$가 되게 조정하여, IL-I 을 0.001, 0.01, 0.1 또한 1 ng/$m\ell$를 첨가하여 농도에 따른 효과를 조사하였고, 각각 24, 48, 72, 96 또한 120시간을 배양하여 시간에 의한 효과도 실시하였다. 각 배치구에서 얻어진 배양액은 TGF-$\beta$를 조사하기 전까지 -2$0^{\circ}C$에 동결 보존하였다. TGF-$\beta$의 측정은 TGF-$\beta$ kit(promega, USA)를 이용하여 실시하였으며, 통계학적 분석은 Anova test를 Statview program을 이용하여 분석하였다. 시험의 결과 대조구에 비해 IL-I 첨가구는 2-3배의 TGF-$\beta$생산을 보였으며, 배양시간에 따른 생산은 시간이 지남에 따라 약간 상승하는 경향을 보였으나, 유의적인 차이를 보이지는 않았다. 또한 IL-I의 농도에 따른 생산의 변화는 IL-I의 농도에 따라 약간의 차이를 보였고 유의적인 차이는 인정되지 않았다. 임신 및 비임신의 경우 임신우의 비장 macrophage가 비임신보다는 약간 상승하는 거스로 나타났다. 이상의 결과로 볼 때 IL-I $\alpha$ 는$\bet

• Journal of the Korea Academia-Industrial cooperation Society
• /
• v.13 no.1
• /
• pp.425-430
• /
• 2012

By the theories of the physics, the phenomena in the world are understood. And also these theories gave the influence to design the architecture. In this research, the contents which the thought of the physics gives a influence a building design is considered, and try to suggest the interrelation between the physics and architectural design. As the result, the architectural design on the influence of classical physics is based on the homogeneous space and the design focus on the style or form of the building with a geometry plan which is not concerned of the place. On the other hand, contemporary architectural design is based on the thought of the modern physics. By this concepts, the architecture is understood as the area contain the conditions of the circumstance and by interaction between buildings and the environment and by various viewpoints like cubism. And the architecture which is settled recently is shown of the diversity and atypical form.

• Journal of the Korea Institute of Information and Communication Engineering
• /
• v.21 no.4
• /
• pp.811-819
• /
• 2017

A behavior pattern analysis algorithm based on descriptors consists of information of a moving object and temporal histogram is proposed. Background learning is performed first for detecting, tracking and analyzing moving objects. Each object is identified using an association of the center of gravity of objects and tracked individually. A temporal histogram represents a motion pattern using positions of the center of gravity and time stamp of objects. The characteristic and behavior of objects are figured out by comparing each coordinates of a position history in the histogram. Behavior information which is comprised with numbers of a start and end frame, and coordinates of positions of objects is stored and managed in the linked list. Descriptors are made with the stored information and the video retrieval algorithm is designed. We confirmed the higher retrieval accuracy compare with conventional methods.

• Proceedings of the Korean Institute of Information and Commucation Sciences Conference
• /
• 2015.05a
• /
• pp.571-575
• /
• 2015

This paper propose a temporal histogram -based behavior pattern analysis algorithm to analyze the movement features of moving objects from the image inputted in real-time. For the purpose of tracking and analysis of moving objects, it needs to be performed background learning which separated moving objects from the background. Moving object is extracted as a background learning after identifying the object by using the center of gravity and the coordinate correlation is performed by the object tracking. The start frame of each of the tracked object, the end frame, the coordinates information and size information are stored and managed by the linked list. Temporal histogram defines movement features pattern using x, y coordinates based on time axis, it compares each coordinates of objects for understanding its movement features and behavior pattern. Behavior pattern analysis system based on temporal histogram confirmed high tracking rate over 95% with sustaining high processing speed 45~50fps through the demo experiment.

• The Journal of the Korea Contents Association
• /
• v.15 no.12
• /
• pp.370-377
• /
• 2015

This study attempted to examine the impact of musculoskeletal pain on pedestrian crossing using a sample that consisted of 282 aged people. The research method involved an examination of the musculoskeletal pain of the elderly using a musculoskeletal questionnaire. The subjects were also told to walk across four- and six-lane pedestrian crossings while their gait velocity, step count, and step time were measured. The research results for pain by body regions indicated that waist pain had the highest complaint rate(33.3%). When there was musculoskeletal pain in the waist and leg/foot, gait velocity also decreased and step count and step time increased. However, usage of a cane turned out to have a positive impact on pedestrian crossing. Nevertheless, no significant relationship between waist and leg/foot pain and pedestrian crossing characteristics was found. We expect there will be continuous further studies on the subject of diverse physical problems of the aged and pedestrian crossing.

• Annual Conference of KIPS
• /
• 2014.04a
• /
• pp.889-891
• /
• 2014

인구 고령화의 빠른 진행과 건강보험 정책의 공공성 기조 확대, 전반적인 국민들의 소득수준이 향상 되면서 건강한 삶에 대한 관심이 증가하고 있다. 따라서 의약품의 수요도 함께 증가하고 있다. 의약품의 복용은 사람의 건강과 직접적인 연관이 있어 복용자는 약품에 대한 정보를 알고 복용을 해야 한다. 기존에 존재하던 헬스케어 전문업체가 개발한 약품검색 어플리케이션은 사용자에게 너무 많은 정보를 요구 함 으로써 편의성을 제공하지 못할뿐더러 약품을 검색 하는데 오랜 시간이 소요된다. 본 논문은 이러한 단점을 보완하여 사용자에게 최대한의 편의성을 제공하는 어플리케이션에 대하여 소개한다. 이 어플리케이션은 사용자가 스마트기기를 이용하여 약품을 찍기만 하면 약품에 대한 정보를 출력해준다.

• The Journal of the Institute of Internet, Broadcasting and Communication
• /
• v.9 no.5
• /
• pp.205-211
• /
• 2009

The Weightless Neural Network(WNN) has the advantage of the processing speed, less computability than weighted neural network which readjusts the weight. Especially, The behavior information such as sequential gesture has many serial correlation. So, It is required the high computability and processing time to recognize. To solve these problem, Many algorithms used that added preprocessing and hardware interface device to reduce the computability and speed. In this paper, we proposed the Ram based Sequential Cumulative Neural Network(SCNN) model which is sign language recognition system without preprocessing and hardware interface. We experimented with using compound words in continuous korean sign language which was input binary image with edge detection from camera. The recognition system of sign language without preprocessing got 93% recognition rate.

• Radiation Oncology Journal
• /
• v.18 no.1
• /
• pp.51-58
• /
• 2000

Purpose :The effect of PTK inhibitors (herbimycin A and genistein) on the induction of radiation-induced apoptosis in Ph-positive KS62 leukemia cell line was investigated. Materials and Methods :K562 cells in exponential growth phase were irradiated with a linear accelerator at room temperature. For 6 MV X-ray irradiation and drug treatment, cultures were initiated at 2×106 cells/mL. The cells were irradiated with 10 Gy. Stock solutions of herbimycin A and genistein were prepared in dimethyl sulphoxide (DMSO). After incubation at 37$^{\circ}C$ for 0$\~$48 h, the extent of apoptosis was determined using agarose gel electrophoresis and TUNEL assay. The progression of cells through the cell cycle after irradiation and drug treatment was also determined with flow cytometry. Western blot analysis was used to monitor bel-2, bel-X$_{L}$ and bax protein levels. Results :Treatment with 10 Gy X-irradiation did not result in the induction of apoptosis. The HMA alone (500 nM) also failed to induce apoptosis. By contrast, incubation of K562 cells with HMA after irradiation resulted in a substantial induction of nuclear condensation and fragmentation by agarose gel electro-phoresis and TUNEL assay. Genistein failed to enhance the ability of X-irradiation to induce DNA fragmentation. Enhancement of apoptosis by HMA was not attributable to downregulation of the bel-2 or bel-X$_{L}$ anti-apoptotic proteins. When the cells were irradiated and maintained with HMA, the percentage of cells in G2/M phase decreased to 30$\~$40$\%$ at 48 h. On the other hand, cells exposed to 10 Gy X-irradiation alone or maintained with genistein did not show marked cell cycle redistribution. Conclusion : We have shown that nanomolar concentrations of the PTK inhibitor HMA synergize with X-irradiation in inducing the apoptosis in Ph (+) K562 leukemia cell line. While, genistein, a PTK inhibitor which is not selective for p210$^{bcr/abl}$ failed to enhance the radiation induced apoptosis in KS62 cells. It is unlikely that the ability of HMA to enhance apoptosis in K562 cells is attributable to bel-2 family. It is plausible that the relationship between cell cycle delays and cell death is essential for drug development based on molecular targeting designed to modify radiation-induced apoptosis.

• Journal of the korean academy of Pediatric Dentistry
• /
• v.28 no.3
• /
• pp.363-368
• /
• 2001

It is well known that numerous factors influence the light output of curing units, but many dentists are un aware that the output of their curing lights are inadequate. This study was conducted to evaluate the light in tensity of visible-light curing units in some private dental clinics and hospital dental clinics. In order to determine the maximum light intensity of the curing units, lamps, filters and fiber optic bundles, they were replaced with new ones and light intensity was remeasured. Light intensity was measured by employing a digital radiometer (EFOS model #8000, USA). Light intensity ranged in $29\sim866mW/cm^2$ (below $150mW/cm^2$ ; 17.8%, $150\sim300mW/cm^2$ : 46.6%, above $300mW/cm^2$ ; 35.6%). The replacement of the components increased the light intensity, with maximum increases of 94.8% for lamps, 82.3% for filters, 200.8% for fiber optics and 361.5% for all three parts. According to the manufacturer of radiometer, curing light is considered as unsuitable for use with a reading of above $300mW/cm^2$ by the radiometer. Applying these criteria to the present study, 64.4% of the curing units required repair or replacement. The results of this study indicated that the light intensities of the curing units used in dental practice were lower than optimum level.

• Korean Journal of Microbiology
• /
• v.28 no.1
• /
• pp.55-64
• /
• 1990

Cell volume and DNA contents of the fusants were similar to those of parents. Genetic stability of the fusants was increased when they were cultured on minimal medium (MM) rather than on complete medium (CM), and the fusants were stabilized by subculturing 7 generations each 7 day on MM agar. The finally selected fusants after being cultured for 6 months on CM were stable without segregation. The fusants could also form nuclein and ascospores, and show red and pink colors by the test of TTC colorization. Assimilability and fermentability of carbon sources of the fusants were similarto those of parents. The tolerance of KCl, NaCl, sodium propionate and cycloheximide showed the traits of one strain of parents. When the fusants were cultured for 72 hr and 60 hr in the medium containing 20% glucose and sucrose, respectively, the yield of ethanol for FWKS 260 was reached to 9.6 v/v% and 9.8v/v%, respectively. The sensitive strain Kyokai 7 was found to be killed entirely after cultivation of 48 hr by the killer toxin from the fusants. The recipient S 29 and Kyokai 7 were found to have neither L nor M dsRNA plasmid. However, K 52 and fusants had both L and M dsRNA plasmid of 4.7 kb and 2.5 kb, respectively. The curants treated by heat and cycloheximide did not contain M dsRNA plasmid, but had large amounts of L dsRNA plasmid of those of killer yeasts.