• Title/Summary/Keyword: 순화

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Acclimatization and Growth Characteristics of Plantlets of Eleutherococcus senticosus Maxim Cultured by Bioreactor (생물반응기에서 배양한 가시오갈피 유식물체의 순화 및 생육특성)

  • Li, Cheng-Hao;Lim, Jung-Dae;Kim, Myong-Jo;Kim, Na-Young;Yu, Chang-Yeon
    • Korean Journal of Medicinal Crop Science
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    • v.13 no.4
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    • pp.133-137
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    • 2005
  • Experiments were conducted to find the optimal acclimatization conditions for Eleutherococcus senticosus plantlets regenerated from bioreactor cultured somatic embryos, various acclimatizing conditions were compared regarding both survival rate and growth of the plantlets. Among the various temperature and artificial soil tested, the highest survival rate (88%) was observed when plantlets were acclimatized in Klasmann bed soil at $10^{\circ}C$ When in vitro plantlets directly transplanted to field environment, shading treatment was necessary and 50% shading was more effective than 30% shading. Transplanting season were also important for successful acclimatization of in vitro cultured plantlets, transplanted on March 20 with 50% shading exhibited the best survival rate and further growth.

Serum Enzyme and Isozyme Activities of Rats Acclimated to Cold Environment (寒冷環境에 순화시킨 흰쥐의 血淸酵素 및 同位酵素의 活性)

  • 정애순;남상열
    • The Korean Journal of Zoology
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    • v.29 no.2
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    • pp.107-120
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    • 1986
  • The activities of serum of serum lactate dehydrogenase (SLDH), serum alkaline phosphatase (SALP), serum creatine phosphokinase (SCPK), and their isozymes were determined in adult male Sprague-Dawley rats acclimated to cold environment $(4\\pm1^\\circC)$ for 36 days. The SLDH activity was significantly higher in the early stage of acclimated period. The steady state of SLDH activity seemed to be reached by the end of acclimated period. Electrophoretic separation of serum of control rat showed three SLDH isozymes. Isozymes SLDH4 and SLDH5 appeared most prominently, whereas only trace of SLDH1 or SLDH2 was found. The increase in SLDH level during acclimation to cold environment is a reflection of an immediate increase in the SLDH1, SLDH2, and SLDH3 type of SLDH isozyme. The acclimation to cold environment increased significantly level of SALP in the early state of acclimated period. SALP activity showed a attaining steady state with the resting level after transient rise. Electrophoretic separation of SALP of control rats showed the SALP1 and SALP2 fractions. The transient rise in SALP activity of rats acclimated to cold environment coincided with a transient rise in SALP1 fraction. Immediately after exposure to cold environment, there was significant elevation in SCPK activity. Value returned to normal after transient rise. A new steady state of SCPK activity seemed to be reached by 36 days. It may be inferred from the above data that thermal compensation appears to result from a change in the activity of an enzyme and that the SLDH, SLDH-isozyme, SALP-isozyme, and SCPK may be involved directly or indirectly in thermoregulation during acclimation to cold environment.

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Influence of Increased Temperature on the Standard Metabolism in the Marine Bivalves Acclimated to Seasonal Water Temperature -I. Effects of Acclimation Temperature (해산패류의 계절별 표준대사에 미치는 승온 효과 -I. 순화온도의 영향-)

  • Kim Kyoung Sun;Chin Pyung
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.35 no.5
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    • pp.463-468
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    • 2002
  • Influence of increased temperature on the standard metabolism in three species of marine bivalves, Crassostrea gigas, Ruditapes philippinarum and Mytilus edulis, acclimated to seasonal water temperatures and collected from the south coast of Korea, were examined in the laboratory. The standard oxygen consumption and filtration rates in the 3 species were measured respectively at the experimental temperature, 4, 7 and 10$^{\circ}C$ or 3, 6 and 9$^{\circ}C$ higher than the mean seasonal water temperature. When the experimental temperatures were higher than the seasonal water temperature, the rates of C. gigas decreased in autumn and spring, and increased In winter, while there was thermal stress in summer. The rates of R. philippinarum increased in spring when the experimental temperatures were 3$^{\circ}C$ and 6$^{\circ}C$ higher than the seasonal water temperature, but the rates increased in autumn and winter when the experimental temperature was even 9$^{\circ}C$ higher than the seasonal water temperature. In summer. metabolic activities of R. philippinarum decreased significantly at temperature higher than acclimation temperature. The rates of M. edulis increased in spring when the experimental temperatures were 3$^{\circ}C$ higher than the seasonal water temperature but the rates were stressed by the increased temperature above 3$^{\circ}C$. In winter, increased temperature did not affect the metabolic activities of M. edulis. These results suggested that the standard metabolism of the three marine bivalves in summer was stressed by the increased temperature, whereas the metabolism was activated in winter.

알아둡시다-식생활용어 3백41개 순화

  • Korea Dairy Industries Association
    • 우유
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    • s.51 winter
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    • pp.44-45
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    • 1992
  • 문화부는 최근 요식업소나 각종요리 관련서적, 강좌 등에서 사용되고 있는 식생활관련 외래어 및 잘못된 표기의 개정사항을 발표했다. 이번에 개정된 식생활 관련용어는 국립국어연구원이 지난해부터 시장 · 백화점 서울시내 80여개 대중음식점과 각종요리책에 있는 식생활 관련용어 4천여 개를 조사 연구해 순화대상을 선정한 후 국어심의회 심의를 거쳐 확정했다. 다음은 순화된 용어들이다.

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부유상의 Batch식 배양에서의 암모니아 및 아질산염의 제거특성

  • Jo, Sun-Ja;Jeong, Yong-Ju;Lee, Na-Eun;Son, Hyeong-Sik;Cha, Mi-Seon;Lee, Sang-Jun
    • 한국생물공학회:학술대회논문집
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    • 2003.04a
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    • pp.348-350
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    • 2003
  • Using acclimated sludges with inorganic nitrogen medium for three months and raw sludges, we investigated characteristics of biological nitrogen removal. As results, the acclimated sludges accomplished nitrification efficiently. But raw sludges have hardly done ammonium-nitrification and there was a increasing of ammonium concentration in flask with raw sludges for established experimental period of 72 hrs.

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A Study on acclimatization culture of the tissue cultured seedings in Zantedschia spp. (유색칼라 기내배양묘의 순화재배에 관한 연구)

  • Park, N.B.
    • Journal of Practical Agriculture & Fisheries Research
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    • v.5 no.1
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    • pp.20-27
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    • 2003
  • This experiment was carried out to develop an acclimatization system of tissue cultured seedling in Zantedeschia spp. There was not different in percentage of leaf emergence by the media compost of acclimatization but tuber was enlarged in sand(50%)+peatmoss(50%) treatment after 6 months growth. The optimum time for the acclimatizantion of Zantedeschia spp. was from the 20th of March to 20th of April. During this time, rate of acclimatization reached over 90 percentage. However on the 20th of august, acclimatization was low to 64-70.4 percentage. Size of tuber was enlarged in planting on the 20th of March. The weight of tuber was better in 10×10cm density of planting than others.

Studies on Purification and Serology of Rice Dwarf Virus (벼 오갈병 바이러스의 순화와 항혈청 제조에 관한 연구)

  • Lee Soon Hyung;Lee Key Woon;Chung Bong Jo;Halliwell R. H.
    • Korean journal of applied entomology
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    • v.16 no.1 s.30
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    • pp.65-67
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    • 1977
  • Yield losses from rice dwarf virus infection are significant in Korea. Rice dwarf virus(RDV) was purified and RDV-antiserum was produced. The purified virus, mixed with an adjuvant(1:1) was injected every 10 to 14 days into rabbits. Three injections .were sufficient to produce an antiserum of 1/4,096 titer. The produced antisera will be used to facilitate the detection and identification of RDV in rice plants and in the RDV leafhopper vectors.

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Studies on Purification and Serology of Potato Virus X (감자바이러스 X의 순화와 혈청학적 연구)

  • Lee Soon Hyung;Lee Key Woon;Chung Bong Jo
    • Korean journal of applied entomology
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    • v.16 no.2 s.31
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    • pp.101-104
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    • 1977
  • Potato virus X was purified especially for the preparation of antisera for diagnosis and identification. Potato virus X was isolated Iron infected plants by means of indicator plants and identified in electron microscopy. Isolated PVX was multiplied in tomato plants and purified by a modified procedures. The purity of PVX was 0.59mg/m1. Purified PVX was injected into rabbits once a week for 5 weeks. Antiserum was collected 10 days after the last injection. Produced antiserum was determined 1/1024 titers by means of microprecipitin tests and showed sharp reactions in agar gel-diffusion tests.

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