Korean journal of applied entomology (한국응용곤충학회지)
- Volume 16 Issue 1 Serial No. 30
- /
- Pages.65-67
- /
- 1977
- /
- 1225-0171(pISSN)
- /
- 2287-545X(eISSN)
Studies on Purification and Serology of Rice Dwarf Virus
벼 오갈병 바이러스의 순화와 항혈청 제조에 관한 연구
- Lee Soon Hyung (Department of Plant Pathology, Institute of Agricultural Sciences, Office of Rural Development) ;
- Lee Key Woon (Department of Plant Pathology, Institute of Agricultural Sciences, Office of Rural Development) ;
- Chung Bong Jo (Department of Plant Pathology, Institute of Agricultural Sciences, Office of Rural Development) ;
- Halliwell R. H. (Department of Plant Sciences, Texas A & M University, College Station)
- Published : 1977.03.01
Abstract
Yield losses from rice dwarf virus infection are significant in Korea. Rice dwarf virus(RDV) was purified and RDV-antiserum was produced. The purified virus, mixed with an adjuvant(1:1) was injected every 10 to 14 days into rabbits. Three injections .were sufficient to produce an antiserum of 1/4,096 titer. The produced antisera will be used to facilitate the detection and identification of RDV in rice plants and in the RDV leafhopper vectors.
우리나라 중부이남 지역에서 벼 오갈병에 의한 피가 현저히 늘어나고 있는 실정이다. 본시험에서는 매개충을 사용, 순수분리하고 이를 접종하여 증식한 후 Toyoda 등의 순화방법을 개선하여 순화하였다. 그 결과 순화된 바이러스 함량은 ml당 3.12mg 이었다. 순화된 바이러스를 Adjuvant와 함께 토끼에 10-14일 간격으로 5회 주사하여 항혈청을 제조한 결과 1/4,096의 높은 역가를 나타내는 항혈청이 제조되었다.
Keywords