• Title/Summary/Keyword: 숙련구분

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A Comparative Study on Quantifying Uncertainty of Vitamin A Determination in Infant Formula by HPLC (HPLC에 의한 조제분유 중 비타민 A 함량 분석의 측정불확도 비교산정)

  • Lee, Hong-Min;Kwak, Byung-Man;Ahn, Jang-Hyuk;Jeon, Tae-Hong
    • Korean Journal of Food Science and Technology
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    • v.40 no.2
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    • pp.152-159
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    • 2008
  • The purpose of this study was to determine the accurate quantification of vitamin A in infant formula by comparing two different standard stock solutions as well as various sample weights using high performance liquid chromatography. The sources of uncertainty in measurement, such as sample weight, final smaple vloume, and the instrumental results, were identified and used as parameters to determine the combined standard uncertainty based on GUM(guide to the expression of uncertainty in measurement) and the Draft EURACHEM/CITAC Guide. The uncertainty components in measuring were identified as standard weight, purity, molecular weight, dilution of the standard solution, calibration curve, recovery, reproducibility, sample weight, and final sample volume. Each uncertainty component was evaluated for type A and type B and included to calculate the combined uncertainty. The analytical results and combined standard uncertainties of vitamin A according to the two different methods of stock solution preparation were 627 ${\pm}$ 33 ${\mu}$g R.E./100 g for 1,000 mg/L of stock solution, and 627 ${\pm}$ 49 ${\mu}$g R.E./100 g for 100 mg/L of stock solution. The analytical results and combined standard uncertainties of vitamin A according to the various sample weighs were 622 ${\pm}$ 48 ${\mu}$g R.E./100 g, 627 ${\pm}$ 33 ${\mu}$g R.E./100 g, and 491 ${\pm}$ 23 ${\mu}$g R.E./100 g for 1 g, 2 g, and 5 g of sampling, respectively. These data indicate that the preparation method of standard stock solution and the smaple amount were main sources of uncertainty in the analysis results for vitamin A. Preparing 1,000 mg/L of stock solution for standard material sampling rather than 100 mg, and sampling not more than 2 g of infant formula, would be effective for reducing differences in the results as well as uncertainty.

Trial for Drug Susceptibility Testing of Mycobacterium tuberculosis with Live and Dead Cell Differentiation (세포 염색 방법을 이용한 결핵균 감수성 검사법)

  • Ryu, Sung-Weon;Kim, Hyun-Ho;Bang, Mun-Nam;Park, Young-Kil;Park, Sue-Nie;Shim, Young-Soo;Kang, Seongman;Bai, Gill-Han
    • Tuberculosis and Respiratory Diseases
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    • v.56 no.3
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    • pp.261-268
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    • 2004
  • Background : The resurgence of tuberculosis and outbreaks of multidrug resistant (MDR) tuberculosis have increased the emphasis for the development of new susceptibility testing of the Mycobacterium tuberculosis for the effective treatment and control of the disease. Conventional drug susceptibility testings, such as those using egg-based or agar-based media have some limits, such as the time required and difficulties in determining critical inhibitory concentrations, but these are still being used in many diagnostic laboratories because of no better lternatives, considering cost and accuracy. To overcome these limits, a rapid and simple method for new susceptibility testing, using live and dead assays, was applied for a bacterial cell viability assay to distinguish dead from live bacterial cells based on two-color fluorescence. Materials and Methods Strains : Forty strains were used in this study, 20 susceptible to all antituberculosis drugs and the other 20 resistant to the four first line antituberculosis drugs isoniazid, rifampicin, streptomycin and ethambutol. Antibiotics : The four antibiotics were dissolved in 7H9 broth to make the following solutions: $0.1{\mu}g\;isoniazid(INH)/m{\ell}$, $0.4{\mu}g\;rifampicin(RMP)/m{\ell}$, $4.0{\mu}g\;streptomycin(SM)/m{\ell}$ and $4.0{\mu}g\;ethambutol(EMB)/m{\ell}$. Results : Live and dead Mycobacterium tuberculosis cells fluoresced green and red with the acridin (Syto 9) and propidium treatments, respectively. These results are very well accorded with conventional drug susceptibility testing by proportional method on Lowensen-Jensen media (L-J) containing 4 drugs (INH, RMP, EMB and SM), showing a 93.7 % accordance rate in susceptible strains and 95% in resistant strains. Conclusion : The results of the drug susceptibility testing using the live and dead bacterial cell assay showed high accordance rates compared with the conventional proportion method on L-J. This finding suggests that the live and dead bacterial cell assay can be used as an alternative to conventional drug susceptibility testing for M. tuberculosis strains.

Classification of the Aged Distribution and the Occupational-Demographic Characteristics in the Seoul Metropolitan Area (수도권 고령층 분포지역의 유형화와 유형별 거주 및 고용 특성 분석)

  • Park, So Hyun;Lee, Keumsook
    • Journal of the Korean Regional Science Association
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    • v.33 no.3
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    • pp.79-100
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    • 2017
  • This study provides the insight into the aged employment provision issue for the aged-low growth era. For the purpose, we analyze the national trend of the age demographic and occupational employment in first. And then we investigate the spatial characteristics of employment of the aged in the Seoul Metropolitan area which has the highest elderly population by utilizing location quotient, factor analysis, and K-means cluster analysis. As the result, we found that the spatial distribution patterns of the residence and workplace of the elderly were nearly coincided with each other. Furthermore, five clusters of the aged distribution have been determined according to the industrial-occupational-demographic attributes. The result revealed clear spatial segrmentation: Most of elderly population of the research area have been engaged in the low-level service jobs, while elderly population employed to the educated-knowledged based high-level jobs has been distributed at a few cores. The results could be applied to the practical use for regional employment planning for the aged.

Automated Velocity Measurement Technique for Unconsolidated Marine Sediment (해양퇴적물의 자동음파전달속도 측정장치)

  • Kim, Dae-Choul;Kim, Gil-Young;Seo, Young-Kyo;Ha, Deock-Ho;Ha, In-Chul;Yoon, Young-Seok;Kim, Jeng-Chang
    • The Sea:JOURNAL OF THE KOREAN SOCIETY OF OCEANOGRAPHY
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    • v.4 no.4
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    • pp.400-404
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    • 1999
  • The conventional mercury delay method to measure compressional wave velocity of unconsolidated sediment is inconvenient because the signal must be analyzed on the oscilloscope and the mercury column has to be calibrated between measurements. We developed an automated compressional wave velocity measurement technique by connecting an oscilloscope and a PC with a GPIB (General Purpose Interface Bus) card. The GPIB card buses signals from the oscilloscope to the PC where the signal from a sample is analyzed and compared to the input pulse thereby the compressional wave velocity of the sample is computed and recorded automatically. Differences between the mercury delay method and the automated measurement technique are negligible except the slightly greater velocity in the automated measurement technique. We concluded that the new technique can be used to measure the velocity for unconsolidated marine sediment. It also has an advantage to calculate sediment attenuation through the processing of waveform using the spectral ratio technique.

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Splitting between Region of Chromatic and Achromatic by Brightness and Chroma (명암과 채도에 의한 색상영역과 비색상영역의 분할)

  • Kwak, Nae-Joung;Hwang, Jae-Ho
    • The Journal of the Korea Contents Association
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    • v.10 no.7
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    • pp.107-114
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    • 2010
  • Color is a sense signal for human to perceive being through light, and the color is divided into chromatic color and achromatic color. Chromatic color has hue, intensity, and saturation, but achromatic color has only intensity among the properties of chromatic color and doesn't have hue and saturation. Therefore it is important to split colors of image into area for human to perceive colors and not to perceive ones based on vision of human being. In this paper, we find a function to split colors of image into chromatic region of chromatic color region and achromatic region of achromatic color region. First, the input image of RGB color space is converted into the image of HSI color space in consideration of human vision and get a binary image from the converted image. After then, a function to split colors into ROC(ROC: Region of chromatic.) and ROA(ROA:Region of achromatic) is yield. It is difficult to split color of a general image into ROC and ROA. Therefore, to get the chromatic area and achromatic area, we make gradient images to have all range of intensity and range of saturation and to have a little range of hue and yield the function. The evaluation is tested using subjective-quality by 50 non-experts for result images of test images and general images. The results of the proposed method get better 27.5~32.96% than these of the conventional method

Selection of Routes for Reflecting Driver's Characteristics by Adopting Multi-Attribute Utility Theory (MAUT) (다속성 효용이론을 적용한 운전자 특성별 경로 선택 연구)

  • Oh, Ji-Eun;Bae, Sang-Hoon
    • The Journal of The Korea Institute of Intelligent Transport Systems
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    • v.10 no.3
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    • pp.25-35
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    • 2011
  • Traffic volume increases due to diversification of industry. Also, Automobile ownerships also increase steadily. It is estimated that the registered number of vehicle is expected to be 20 milion in the year 2015. These trends may result in increasing the number of woman drivers and elderly drivers. Therefore, this study aims to identify routes that reflect characteristics of each driver's preferences. A survey was conducted on different routes attributes for variances drivers. Driver types were classified by gender, age, and driving career. Accordingly, a weight for road composition attribute such as number of lanes, number of accidents, slope was estimated by using Swing Weighting technique in Multi-Attribute Utility Theory. In addition, a case study was conducted and identified weights were applied to routes. In result, drivers commonly prefer short route when they considered their routes. Also, male drivers prefer speedy and shorter route than that of female drivers. Elderly drivers prefer safe routes that represent low accidents rate. Moreover driving career under a year drivers prefer safe and easy routes. Therefore, we may conclude that the necessity of diversified route information is essential in the future car navigation system.

Middle-Age' Entrepreneurship & Re-Employment Education Influences Re-Employment Anxiety, Re-Employment Stress, Entrepreneurial Intention (창업 및 재취업 교육이 재취업불안, 재취업스트레스, 창업의도에 미치는 영향)

  • Kim, Seo-ho;Byun, Sang hae
    • Asia-Pacific Journal of Business Venturing and Entrepreneurship
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    • v.11 no.6
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    • pp.71-81
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    • 2016
  • Sothe Korea that entered in to aging society in year 2000 according to the entrance in 2017 as in log-term the young work focrce decrease and skilled elder populatin decrease became important issue of national entrepreneurship & re-employment of middle-age' has become the topic of social problems. The purpose of this study was to examine the impact of a entrepreneurship & re-employment education to job-seeking anxiety, job-seeking stress and entrepreneurial intention in an effort to prove the effectiveness of entrepreneurship & re-employment education. The subjects in this study were 67 believer who were of G church in the city of K. A entrepreneurship & re-employment education was conducted in fourteen sessions, once a week. The major findings of the study were as follows: First, the experimental group who participated in the entrepreneurship & re-employment education felt less job-seeking anxiety. Second, the experimental group who participated in the entrepreneurship & re-employment education felt less job-seeking stress. Third, the experimental group who participated in the entrepreneurship & re-employment education showed improvement in entrepreneurial intention.

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Characteristics of Classified Congenital Muscular Torticollis Applied Therapeutic Massage and Manual Stretch Therapy During 6 Months (6개월간의 치료적 마사지와 도수신장 치료에 대한 선천성 근성 사경 환아의 유형별 특성)

  • Kim, Yong-Wook;Kim, Min-Hee
    • The Journal of the Korea Contents Association
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    • v.10 no.4
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    • pp.216-224
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    • 2010
  • The main purpose of this prospective longitudinal study was to evaluate, according to a standard clinical classification and assessment method, the effect of a standardized manual stretching and therapeutic massage program on patients with clearly defined congenital muscular torticollis. A total of 52 cases of congenital muscular torticollis who were first seen when they were less than one year old and were treated with conservative physical therapy. Before treatment, the patients were divided into one of three clinical groups: (1) sternomastoid tumor group, (2) muscular torticollis group, and (3) postural torticollis group. Of the 52 patients, 32 (61.5%) made up the sternomastoid tumor group; 15 (28.9%) the muscular torticollis group; and 5 (9.6%) the postural torticollis group. Multivariate analysis of the outcomes showed that the duration of treatment was significantly differentiated according to the clinical group (p<0.01), age at presentation (p<0.01), and a passive rotation deficit of the neck (p<0.01). The current study showed that well controlled conservative physical therapy is safe and effective in the treatment of congenital torticollis in about 84.6% of patients who are first seen before the age of one year.

Identification of Secondary Chemistry Teachers' Ability to Carry-out Experimentation (화학 교사에게 필요한 실험 능력)

  • Park, Hyun-Ju;Jeong, Dae-Hong;Noh, Suk-Goo;Lim, Hee-Jun;Han, Jae-Young;Park, Jong-Yoon
    • Journal of the Korean Chemical Society
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    • v.53 no.6
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    • pp.765-773
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    • 2009
  • The purpose of this study is to identify secondary chemistry teachers' abilities to carry-out experiment lesson successfully. As far as the research method is concerned, literature reviews, analyses of abilities to carry-out experimentation from science textbooks, and a survey of science teachers' perceptions of experimental ability were employed along with the progressive discussions among the authors. In order to identify secondary chemistry teachers' abilities to carry-out experimentation, we divided the experiment lesson into three stages of preparation, conduct and arrangement, and management of lab, and added the stage of safety. Each stage is classified into sub-areas, and the sub-area consists of subordinate elements. The safety stage was included separately to emphasize the importance of the safety issue in lab and experimental activities. The secondary chemistry teachers' abilities to carry-out experimentation are the abilities to perform experiments in person, presupposing the instruction of experiment, and can be featured with the use of reagent and measuring instruments, the preparation of sample, designing experiment, correct experimental habit and skillfulness, data processing, analysis and reasoning, and management of lab and safety.

The Studies on Pregnancy after Transfer to Recipient with Blastocyst Derived In Vitro in Korean Cattle (한우 유래의 체외수정란의 이식후 임신에 관한 연구)

  • 김소섭;최석화;김재명;이제협;김재영;박흠대
    • Journal of Embryo Transfer
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    • v.18 no.3
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    • pp.237-242
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    • 2003
  • These studies were carried out to establish an effective in vitro embryo transfer methods by analyzing several factors. The base media were TCM-199 solution for in vitro maturation(IVM) of bovine follicular oocytes and Fer-TALP solution for in vitro fertilization(IVF) and CRlaa medium for in vitro culture(IVC). IVC used the fertilized oocytes of 24-hr culture (day 1)after IVF. Embryos were cultured in drop-culture that contained 25 embryos per 10${mu}ell$. Blastocysts cultured for 7 to 9 in vitro were transferred to recipients. The results obtained were as follows: 1. The pregnancy rate according to different region of embryo transfer were 33.8%, 48.1%, 45.0% and 35.3% respectively. 2. The pregnancy rate according to the parity of recipient when embryos were transferred to nulliparous (42.9%) was higher than that of 1∼3nd parlous(36.9%), however there were not show significant difference each other. 3. According to the stage of blastocyst, the pregnancy rate when middle blastocysts (MB) (45.5%) were transferred to recipients were higher than that of late blastocysts (LB) (41.0%). 4. When IVF-derived blastocysts cultured for 7 to 9 day were transferred to recipients, the pregnancy rate was higher 7 day of blastocyst than that of 8 day or 9 day of blastocyst. The results of embryo transfer according to the regions, the parity of recipient and the development stage showed that blastocyst formed for 7day transferred to nulliparou were higher pregnancy rate than others.