• Title/Summary/Keyword: 수확후생리

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Isolation and Characterization of Surfactin-producing Bacillus amyloliquefaciens YJ07 from Spent Mushroom (Pleurotus eryngii) Substrates (새송이버섯 수확 후 배지로부터 surfactin 생성 Bacillus amyloliquefaciens YJ07의 분리 및 특성)

  • Shin, Pyung Gyun;Yoo, Young Bok;Cho, Yong Un;Cho, Soo Jeong
    • Journal of Mushroom
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    • v.9 no.4
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    • pp.180-185
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    • 2011
  • Spent mushroom substrates (SMS) is a by-product remaining after a crop of mushrooms. About four surfactin-producing strains were isolated from SMS (Pleurotus eryngii). Among of them, one isolate, which designated to YJ07, potentially showed the antifungal activity against Aspergillus flavus and Aspergillus ochraceous producing mycotoxin on PDA medium. The biochemical characteristics of the strain YJ07 was similar with Bacillus subtilis and Bacillus amyloliquefaciens by Bacillus ID kit and VITEK 2 system. Comparative 16S rDNA gene sequence analysis of the strain YJ07 also showed that the strain YJ07 was most closely related to Bacillus amyloliquefaciens with sequence similarity of 99.5%. On the basis of their biochemical characteristics and phylogenetic distinctiveness, the strain YJ07 was classified within the genus Bacillus as Bacillus amyloliquefaciens YJ07. The antifungal compound from B. amyloliquefaciens YJ07 was similar to lipopeptide surfactin from Bacillus subtilis by TLC and HPLC analysis.

Studies on the Physiological Change of Fruit during Storage and Mineral Content in 'Chojuro' Pear (배 장십랑품종 과실의 수학 후 생리적 변화와 과실부위별 무기성분함량)

  • Lee, Ju-Youn;Choi, Jong-Seung;Ahn, Young-JIk
    • The Journal of Natural Sciences
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    • v.8 no.2
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    • pp.175-179
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    • 1996
  • The fruit soluble solids and acidity decreased during storage regardless of fruit size, fruit firmness decreased also, and the decreasing rate of the firmness in large fruit was higher than small. The ethylene production of fruit was increased during storage, and there was the peak of fruit respiration rate at 2 and 6 weeks after harvest. The calcium and magnesium content was the highest in peel, the lowest in flesh, and intermediate in core. Especially, the calcium content in the outer flesh was lower than in the inner flesh, and the magnesium content in the middle part of flesh was lower than in the other parts. The Ca/Mg ratio in the inner flesh and the K/Ca ratio in the outer flesh were higher than in the other parts.

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Studies on the Flowering and Maturity in Sesame(Sesamum indicum L.) V. Changes of Grain Weight and Germinability by Maturity in Different Plant Types (참깨 개화, 등숙에 관한 연구 V. 참깨의 등숙에 따른 초형별 종실중 및 발아력의 변화)

  • Kang, Chul-Whan;Lee, Jung-Il;Son, Eung-Ryong;Yoo, Chang-Yung
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.30 no.4
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    • pp.436-441
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    • 1985
  • The study was conducted to provide basic information to breeders and agronomists working with sesame. The grain weight and germinability were investigated for eight plant types classified by branching habit, capsules per axil, and carpels and loculi of a capsule. Two typical cultivars were chosen for each plant type among 527 gene pools. Dry weight of one thousand grains was increased rapidly from 25th to 35th day after flowering, and reached peak on 40th day after flowering in upper part capsules and 45th day after flowering in lower and middle part capsules, so that this period was considered to be of physiological maturity in each capsule bearing part. Side capsules on main stem and branch capsules were lighter than central ones of main stem, and upper capsules of four carpels eight loculi type decreased more seriously. BTB type demonstrated relatively better growth compared to the growth of BTQ type in one thousand grain weight. The maximum grain filling duration for germination percentage increased rapidly up to 40th day after flowering. Above 70% germinability was obtained from 40th day after flowering. Harvesting time of physiological maturity was considered to be 45th day after flowering with peaks of 2.14g of one thousand grain weight, 26% of grain water content and 90% of germinability.

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Recent Research Status of Postharvest Management of Broccoli (브로콜리 수확후 관리의 최근 연구 동향)

  • Choi, Ji-Weon;Lee, Woo-Moon;Kwak, Jung-Ho;Kim, Won-Bae;Kim, Ji-Gang;Lee, Seung-Ku;Cho, Mi-Ae
    • Journal of the Korean Society of International Agriculture
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    • v.23 no.5
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    • pp.497-502
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    • 2011
  • Broccoli is considered as one of the functional foods to offer a hearty supply of the nutrient-rich vegetable in the world. Broccoli contains high level of phytochemicals, and that is selected as one of the top 10 vegetables for human health promotion. Especially, glucosinolates and flavonoids are well known as anti free oxygen radicals in vegetables and fruits. In Korea, broccoli consumption has increased to well known on the health-beneficial vegetables since 2000. However, broccoli has many problems of postharvest management since the quality of harvested heads quickly declines. Major problems are the floret yellowing, wilting, off-odor, and decay. The multiple postharvest applications improve broccoli quality and cold treatment including pre-cooling extends on the shelf-life with circumstance of optimum storage which is 0℃ temperature and a range of 95-100% relative humidity. Controlled atmosphere or modified atmosphere packaging can be used as supplemental treatments to extend postharvest life. 1-2% O2 + 5-10% CO2 is currently recommended for broccoli. Postharvest management is important for broccoli because price fluctuations depend on harvest time and quality. In this study, we tried to review physiological change of broccoli after the harvest, storage method, and various techniques to optimize quality during distribution.

Effect of Quality and Yield for Succeeding Crop Cultivation before Potato Harvest in Semi-highland (준고랭지 감자 수확전 후작물 재배가 품질 및 수량에 미치는 영향)

  • Suh, Jong-Taek;Chang, Dong-Chil;Cho, Ji-Hong;Cho, Kwang-Soo;Park, Young-Eun;Kim, Hyun-Jun;Cho, Hyun Mook
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.58 no.3
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    • pp.253-259
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    • 2013
  • This study was conducted to investigate proper mixed cultivation system of potato and succeeding crops (Chinese cabbage and Radish) in 2009. Potato varieties, 'Superior' a middle maturing variety and 'Haryeong' a late maturing variety were used. After potato harvest, succeeding crops of Radish and Chinese cabbage were planted on July 22th in 'Superior' variety plot and Aug. 3rd in 'Haryeong' variety plot. Potato crop was harvested in proper time, after 15, 30, 45 and 60 days. and a suitable potato variety for succeeding crops among 'Superior' and 'Haryeong' was checked. 'Superior' variety was observed to be low rotting, deformity and greening at Chinese cabbage and Radish treatment plot than non-treatment plot. In addition, high yields of potato was maintained in succeeding crop treatments.

Mechanical Property and Ultrasonic Parameter Changes of the Apple Flesh during Storage (저장기간에 따른 사과 과육의 기계적 특성 및 초음파 파라미터)

  • 김기복;김만수;정현모;이상대;박정길
    • Proceedings of the Korean Society for Agricultural Machinery Conference
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    • 2003.02a
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    • pp.463-469
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    • 2003
  • 과실은 재배기술, 재배환경의 영향으로 형상, 색택, 병충해로 인한 상해 정도가 다르게 나타나기 때문에 상품성이 매우 다양하게 결정된다. 또한 수확, 저장, 포장, 수송, 상하차 등 유통과정에서 기계적 손상이 발생할 수 있으며 유통기간이 길어짐에 따라 호흡 및 증산작용 등과 같은 생리적 작용으로 인하여 부패 및 변질이 발생하며 이러한 유통과정 중에서 발생하는 과실의 품질저하가 상당한 수준에 이르고 있으나, 이에 대한 관심은 매우 낮은 실정이다. 또한, 대부분의 청과물은 수확 후 소비되기까지 장기간의 저장으로 품질 변화가 발생할 수도 있다. (중략)

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Combined Foliar Spray of Boron, Calcium, and Silicon can Influence Quality and Shelf Life of Cherry Tomato in Modified Atmosphere Packaging (붕소, 칼슘, 규소의 복합 엽면시비가 방울토마토의 품질과 MAP 조건에서 저장성에 미치는 영향)

  • Islam, Mohammad Zahirul;Mele, Mahmuda Akter;Han, Su Jeong;Kim, Ju Young;Choi, In-Lee;Yoon, Jae Su;Yoon, Hyuk Sung;Park, Jong-Man;Kim, Il-Seop;Choi, Ki-Young;Kang, Ho- Min
    • Journal of Bio-Environment Control
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    • v.26 no.4
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    • pp.310-316
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    • 2017
  • This study was analyzed the effects of boron (B), calcium (Ca), silicon (Si) on quality and shelf life of 'Unicorn' cherry tomato at the light red maturity-stage. The storage conditions were modified atmosphere packaging (MAP) by oxygen transmission rate (OTR) packaging film at $5^{\circ}C$, $11^{\circ}C$, and $24^{\circ}C$. Respiration and ethylene production were the lowest in B + Ca + Si -treated tomato fruits. The lowest fresh weight loss and the longest shelf life resulted from the B + Ca + Si treatment. And the firmness was enhanced by B + Ca + Si treatment at harvest time, and it was retained after storage at $5^{\circ}C$, $11^{\circ}C$, and $24^{\circ}C$. Significantly lower soluble solids, lycopene, and color development were found at B + Ca + Si-treated tomato fruits compared with control after storage. Moreover, the highest titratable acidity and vitamin C content were observed in B + Ca + Si-treated tomato fruits after storage. From the above results, it was concluded that B + Ca + Si combined treatment can delay the maturity of cherry tomato after harvesting, and retained the firmness and prolong the shelf life.

Effect of Ethylene Scrubber in Polyethylene Film Wrap on the Storage of 'Tsugaru' Apples (플라스틱 필름포장 내 에틸렌제거가 사과 '쓰가루' 과실의 저장 품질에 미치는 영향)

  • Hwang, Yong Soo;Chun, Jong Pil;Lee, Jae Chang
    • Korean Journal of Agricultural Science
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    • v.25 no.1
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    • pp.19-25
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    • 1998
  • This study was conducted to develop an appropriate postharvest technique to extend the shelf-life of an early cultivar, 'Tsugaru' apples. The general quality of stored fruit was more affected by maturity at harvest than postharvest treatments. When firmness and internal quality were considered, fruits harvested at the medium stage of color development showed better results. Between postharvest treatments examined in this study, a simple polyethylene film(PE) wrapping (30um) was excellent on inhibition of weight loss but this treatment revealed no clear effect on other quality factors including firmness and acidity. Ethylene scrubber(ES) in polyethylene wrapping showed a positive effect on keeping fruit quality in general. Especially acid content was higher in fruit stored with PE + ES, thus, fruit seemed to be better in freshness. A physiological disorder was observed regardless postharvest treatments. The symptom was associated with incidence of pitting on surface and breakdown of the effected tissue. And much more symptoms were developed near the calyx end. Thus, future studies concerning 'Tsugaru' storage should include an examination of the optimum storage temperature.

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Rubus coreanus Miquel Bioconversion of Metabolites by Fermenting Bacteria and Their Functional Enhancement (복분자 기능성 증대를 위한 유용미생물 활용 생물전환 공정 도입 방안 연구)

  • Lim, Jeong-Muk;Choi, Ui-Lim;Kim, Joong-Oh;Lee, Jeong-Ho;Oh, Byung-Taek
    • Proceedings of the Plant Resources Society of Korea Conference
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    • 2018.04a
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    • pp.65-65
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    • 2018
  • 복분자(Rubus coreanus Miquel)는 anthocyanin, phenolic acid, tannin, flavonoid, stilbenoid와 같은 생리활성물질이 풍부하게 존재하는 것으로 보고되었으며 항염증, 항암활성, 항산화효과 등 다양한 생리활성에 대한 효능이 있어 건강식품으로써 애용되고 있을 뿐만 아니라 약용성 건강보조식품 등으로 각광받고 있다. 생물전환(Bioconversion)은 미생물 또는 효소의 생물학적 촉매 기반 천연소재의 생리활성 물질기능성, 생체이용률, 안전성을 증대시키기 위한 방안으로 많은 연구가 진행되고 있으며 아울러 식품, 의약품, 화장품 등 다양한 분야에서 활성화 되고 있다. 본 연구는 불가사리 발효액으로부터 유산균을 분리하였으며, 유전학적 특성을 확인하기 위하여 16S rDNA 염기서열을 분석하였다. 전북 고창에서 수확된 복분자를 분말상태로 유산균과 발효공정을 수행하였으며, 복분자의 최적 추출조건 선정과 발효공정 전 후의 활성을 관찰하였다. 발효공정 후 추출물의 기능성 평가를 진행하기 위하여 DPPH radical scavenging activity, total polyphenol 함량을 확인하여 항산화 효능 및 유효성분 함량을 평가하였다. 또한 대식세포인 Raw 264.7을 사용하여 MTT assay, Nitric oxide (NO) 생성 억제 효능을 확인하여 세포독성 및 항염증 활성을 평가하였다. 실험결과, 발효기간이 다른 불가사리 비료발효액으로부터 16 종의 다양한 균주를 확보하였으며, 생물전환 공정에 유용 균주를 선정하기 위하여 복분자 분말의 발효공정을 실시한 결과 3 종의 유산균 처리군에서 무처리군 대비 DPPH radical 소거능 및 polyphenol 함량이 증가됨을 확인하였다. 그 중 가장 우수한 활성을 나타내는 균주를 16S rDNA 염기서열 분석한 결과 Lactobacillus coryniformis A6-4로 확인되었으며, 발효공정 후 항산화 활성은 무처리군 대비 약 115%, polyphenol의 함량은 무처리군 대비 약 121%로 증가됨을 확인되었다. 또한 발효공정 후 독성활성이 감소되는 경향을 확인되었으며, 항염증 활성이 유의적으로 증가됨을 확인하였다.

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Characteristics of Processed Food for Improvement Utilization of Sweet Persimmon (단감의 이용성 증진을 위한 특성 검토)

  • Choi, Yoon-Hee;Kim, Eun-Mi;Cho, Yong-Sik;Park, Shin-Young;Yang, Jeong-Min
    • Proceedings of the Korean Society of Community Living Science Conference
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    • 2009.09a
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    • pp.88-88
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    • 2009
  • 단감은 우리나라가 세계에서 가장 많은 양을 생산하는 대표 과일로 과잉생산과 수확기에 일시적인 홍수출하로 인한 가격이 하락하고, 기상이변에 의한 비상품 과일의 생산이 많아지나 전량 폐기되고 있으며, 유통 및 저장 중에 연화현상과 생리적장애로 품질이 저하되어 경제적 손실이 발생되므로 안정적 생산과 소비를 위하여 다양한 형태의 식품개발이 절실히 요구된다. 국내 감 생산량은 352,822톤이며 이 중 떫은감은 146,233톤(41.45%), 단감은 206,589톤(58.55%)으로 단감 생산량이 떫은감에 비하여 많으며 국내 단감 생산지는 진영, 순천, 문산, 담양, 장성 등 집단화 되어 있고, 원료확보가 유리하나 저장은 수확기인 10월 중순, PE필름 봉지에 넣고 밀봉하여 박스 등에 넣어 $0{\sim}1^{\circ}C$의 저온저장고에 이듬해 4월 중순까지 6개월 정도 저장 가능하나 저장비용이 많이 들고, 4월 이후에는 생리적 장해로 보관이 어렵다. 단감은 다른 과일에 비하여 높은 감미와 비타민 A, 비타민 B1, 비타민 C, 식이섬유 및 리코핀 성분이 다량 함유되어 있고, 노화와 암 발생을 억제하는 폴리페놀물질이 풍부하여 건강 기능성 과일로 알려져 있다. 본 연구에서는 시중에서 구입한 단감의 저온저장 중 감모율 및 이용성 구명을 위하여 관련특성을 검토하였다. 단감을 3월에 구입하여 $5^{\circ}C$에 저온저장하여 보관기간별 감모율을 조사하였고, 단감의 전 처리 후 당도, pH, 색도, 수분함량 등을 조사하였다. 3월에 시중에서 구입하여 PE필름에 봉한 채 종이박스 넣어 $5^{\circ}C{\pm}2$ 저온저장고에 저장하여 1개월 후에 조사한 결과 정상과가 75.47%, 25%미만 장해과가 14.37%, 50%이상 장해과가 10.16% 이었고, 2개월 후는 정상과가 41.52%, 25%미만 장해과가 42.88%, 50%이상 장해과가 15.61%이었다. 단감을 꼭지제거, 박피, 제핵 후 절단하여 파쇄 후의 당도는 14.8 $^{\circ}Brix$, pH는 6.0, 수분은 56.7%, 색도 L값은 39.8, a값이 12.6, b값이 22.7이었다. 파쇄한 단감을 $-20^{\circ}C$에 1차 냉동하여 해동 후 당도는 냉동 전 $14.8^{\circ}Brix$에서 $14.2^{\circ}Brix$로, 2차 냉동 해동 후는 $12.6^{\circ}Brix$로 감소하였으며, 색도 L값과 a값 및 b값은 냉동 전에 비하여 감소하였고, 수분함량은 냉동 전 64.4%, 1차 냉동 해동 후에 82.78%, 2차 냉동 해동 후에는 84.09%로 증가하였다.

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