• Korean Journal of Plant Resources
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• v.25 no.1
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• pp.132-141
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• 2012

This study was carried out to test seed germination responses to temperatures and pre-treatments in Hippophae rhamnoides, which has many abilities in antioxidant activity, soil improvement and erosion control. H. rhamnoides seeds were placed at 10, 15, 20, 25, 30 and $35^{\circ}C$ under light condition. As the results, germination percentage (GP) was the highest at 15 and $20^{\circ}C$, and mean germination time (MGT), germination rate (GR) and germination value (GV) were the highest at $25^{\circ}C$. Quadratic and linear regression model were used to determine the cardinal temperatures such as base ($T_b$), maximum ($T_m$) and optimum ($T_o$) temperature for germination. In quadratic regression model using PG, $T_b$, $T_m$ and $T_o$ was estimated as 0.6, 36.4 and $18.5^{\circ}C$, respectively, and temperature range for germination was $35.8^{\circ}C$. In linear regression model using GR, $T_b$, $T_m$ and $T_o$ was estimated as 8.3, 35.4 and $25.3^{\circ}C$, respectively, and temperature range for germination was $27.2^{\circ}C$. Germination properties were investigated after H. rhamnoides seeds were treated by prechilling (1, 2, 4, 6 and 8 weeks), stratification (2, 4, 6 and 8 weeks), solid matrix priming (seed : carrier : water = 5 : 1 : 7, 8, 9 and 10), osmo-priming (-0.25, -0.5, -1.0 and -1.5 MPa) and calcium chloride ($CaCl_2$) -priming (100, 200, 300 and 400 mM). The highest GP was observed in $CaCl_2$ 300 and 400 mM treatments, and MGT was the shortest in stratification 6 and 8 weeks treatments. GR and GV were the highest and GP was the second highest when seeds were prechilled for 1 and 2 weeks. Consequently, prechilling 1 or 2 weeks treatment was considered as the appropriate method when we contemplate qualitative and quantitative effects in seedling production.

• Journal of Life Science
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• v.27 no.9
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• pp.1020-1030
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• 2017

Epidemiology studies have reported a reduced incidence of colon cancer among populations that consume a large quantity of ${\omega}3-polyunsaturated$ fatty acids (${\omega}3-PUFAs$) of marine origin. Herein, we demonstrated a mechanism of anticancer action of ${\omega}3-PUFAs$, showing that they suppressed invasion and tumorigenicity in colon cancer cells. Docosahexaenoic acids (DHA) inhibited the cell growth of HT29 cells. This action likely involved apoptosis, given that the DHA treatment increased the cleaved form of PARP and sub G1 cells. Moreover, the invasiveness of HT29 cells was inhibited following DHA treatment, whereas arachidonic acid (AA) had no effect. The levels of Matrix-metalloproteinase-9 (MMP-9) and MMP-2 mRNA decreased after DHA pretreatment. DHA treatment inhibited MMP-9 and MMP-2 promoter activities and reduced VEGF promoter activity. DHA pretreatment also inhibited the activities of prostaglandin-2 (PGE2)-induced MMPs and the VEGF promoter. Cyclooxygenase-2 (COX-2) overexpression increased the activity of MMPs and that of the Vascular endotherial growth factor (VEGF) promoter in HT29 cells, and DHA inhibited NF-kB and COX-2 promoter reporter activities. As shown by in vivo experiments, when mouse colon cancer cells (MCA38) were implanted into Fat-1 and wild-type mice, both the tumoral size and volume were dramatically inhibited in Fat-1 transgenic mice. Furthermore, TUNEL-positive cells increased in tumors from Fat-1 mice compared with wild mice. In immunohistochemistry, the intensity of CD31 in Fat-1 tumors was weaker. These findings suggest that ${\omega}3-PUFAs$ may inhibit tumorigenicity and angiogenesis as well as cancer cell invasion by suppression of COX-2, MMPs and VEGF via the reduction of NF-kB in colon cancer.

• Korean Journal of Weed Science
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• v.7 no.1
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• pp.90-97
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• 1987

An attempt was done at the Yeongnam Crop Experiment Station in 1986-'87 to obtain herbicide tolerant variant through cell culture. Immatured rice grain was more rapidly and efficiently formed callus in dehulled rice culture method for both rice cultivar types, Tongil type (Indica/Japonica) and Japonica-type. However, Japonica-type cultivar was generally superior than Tongil-type Cultivar in callus formation. Expression rate of herbicide tolerant variant varied depending upon rice cultivar, plant species and herbicide properties. In case of Nagdongbyeo (Japonica) at the first subculture, 46.3% of total callus pieces appeared as herbicide tolerant variant in herbicide media of CGA142464 and followed by NC-311 (11.6%), Butachlor (7.5%), 2.4-D (2.1%), Quinclorac (0.89%), and Propanil (0.25%), in order. This degree of appearance of herbicide tolerant variants rapidly increased as passage of subculture was advanced. Herbicide tolerant callus hardly regenerated as normal plant even though large variations exhibited among culture media.

• Korean Journal of Microbiology
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• v.42 no.3
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• pp.185-194
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• 2006

The aim of this study was to identify the bacteria isolated from endodontic lesions by cell culture and to determine the antimicrobial susceptibility of them against 8 antibiotics. The necrotic pulpal tissues were collected from 27 infected root canals, which were diagnosed as endodontic infection. Samples were collected aseptically from the infected pulpal tissue of the infected root canals using a barbed broach and a paper point. The cut barbed broaches and paper points were transferred to an eppendorf tube containing $500{\mu}l\;of\;1{\times}PBS$. The sample solution was briefly mixed and plated onto a BHI-agar plate containing 5% sheep blood. The agar plates were incubated in a $37^{\circ}C$ anaerobic chamber for 2 to 5 days. The bacteria grown on the agar plates were identified by comparison of 16S rRNA gene (rDNA) sequencing method at the species level. To test the sensitivity of the bacteria isolated from the infected root canals against 8 antibiotics, minimum inhibitory concentrations (MIC) were determined using broth dilution assay. The data showed that 101 bacterial strains were isolated and were identified. Streptococcus spp. (29.7%) and Actinomyces spp. (21.8%) were predominantly isolated. The 9 strains were excluded in antimicrobial susceptibility test because they were lost during the experiment or were not grown in broth culture. The percentage of bacteria susceptible for each antibiotic in this study was clindamycin, 87.0% (80 of 92); tetracycline, 75.0% (69 of 92); cefuroxime axetil, 75.0% (69 of 92); amoxicillin + clavulanic acid (5:1), 71.7% (66 of 92); penicillin G, 66.3% (61 of 92); erythromycin, 66.3% (61 of 92); amoxicillin, 44.6% (41 of 92); and ciprofloxacin, 31.5% (29 of 92). The susceptibility pattern of 8 antibiotics was dependent on the host of the bacteria strains rather than the kinds of bacterial species. These results indicate that antibiotic susceptibility test should be performed when antibiotics are needed for the treatment of infected root canals.

• The Korean Journal of Ecology
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• v.26 no.1
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• pp.39-47
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• 2003

The leaf aqueous extracts from five gymnosperms plant were investigated for allelopathy with five Hibiscus syriacus varieties. The leaf aqueous extract of Pinus rigida had the highest total phenolic compound of 2.21mg/L, whereas the soil under Pinus koraiensis canopy had the highest total phenolic compound of 1.38mg/L. Fourteen phenolic compounds were isolated from five gymnosperm plants by HPLC. Among them, phenolic compounds were the highest in P. rigida (320.56 g/mg) with the primary compound 5-sulfosalicylic acid (312.55 g/mg). The correlation between leaf total phenolic compound and pH was not significant, while the total phenolic compound of the leaf extract changed soil pH. The relative seed germination of H. syriacus varieties showed 25% was threshold concentration. The germination rates of varieties were similar to the control group or showed slight stimulation to treatment of P. koraiensis extract. H. syriacus Cambanha was similar to the control group or showed stimulation in all treated groups. H. syriacus Seohohyang showed stimulation in both root and shoot growth compared to the control group. In other varieties except Seohohyang, shoot growth was similar to the control group, while root growth was stimulated in all treated groups. The extracts of tested gymnosperms showed significantly more stimulation to transplanted Seohohyang seedlings, whereas others were similar to control or inhibited in the greenhouse. The dry weight of Seohohyang was greater in all treated groups than the control group, while other varieties were inhibited. All gymnosperm extracts stimulated the chlorophyll contents of Seohohyang and H. syriacus Koyoro but other varieties were not significantly affected. Accordingly, it is suggested that Seohohyang seems the most desirable when planted within these five gymnosperms.

• Journal of the Korean Institute of Landscape Architecture
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• v.14 no.2
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• pp.7-16
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• 1986

This study was carried out to investigate the effects of such erosion control measures as sowing, planting and small earth structures on the soil and vegetation. In order to study the changes in soil and vegetation, 36 plots were surveyed from 1981 to 1982 in the large erosion control area which is restored last 20 years. The factors which were measured included vegetation coverage, tree growth, number of species, soil depth, soil consistancy, and Chemical properties of soil. The results were as follows; 1) Maximum coverage of the overstory and understory was attained 7 years after the initiation of erosion control. So the overstory need to be tended and pruned. 2) Diversity of species increased until age 6 after which it began to decrease. 3) In order of tree growth, black locust was the fastest, followed by siberian alder and pitch pine. The initial growth of black locust, though the best among the 3 tree stop., decreased rapidly year by year. At the same time, siberian alder and pitch pine grew well until 12 and 6 years after the initiation of erosion control respectively. 4) Fifty percent of the initially planted trees died within 8 yeard. The mortality of siberian alder occurred until the 20th year while the mortality of pitch pine stopped after 10 years. Thereafter 500 trees per hectare were maintained. 5) The soil depth in A and B horision increased by 2cm annually during 20 years. The soil consistency also decreased rapidly until 7th year. The physical soil properties of the rehabilitated areas were improved after the 14th year. 6) The soil pH tend to decrease from 5.3 during the first year to 5.1 during the twentieth year. 7) The organic matter and nitrogen content in the soil were increased by fertilization but after 20 years these nutrients are still deficient for normal tree growth. 8) The phosphorous content in the soil was high in the first year but the longer the period after the initiation of erosion control the lese the content of phosphorous. 9) The biomass of black locust was the highest and increased continuously. The biomass of siberian alder on the contrary decreased from the 15th year because the number of trees in this place was very low. The total biomass in the twentieth year after erosion control initiation was 105.7 ton per hectare.

• Journal of the Korean Institute of Landscape Architecture
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• v.40 no.3
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• pp.81-90
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• 2012

Improvements in the quality of life have resulted in a heightened awareness of safety and the environment. As a result, timber as an environmentally friendly material, is used for landscape facilities and a wide range of purposes. But there are a large number of defects since there are twists and cracks that can be found in wooden landscape facilities. This has led to the use of imported hardwood instead of the Western Hemlock which has been in widespread use. Hardwood is expensive. However, it is being used without any information or research on how much it reduces the actual defects. Construction contractors are in great need of information on the characteristics and defect rates of different types of timber. This study investigated and analyzed the cracks in four types of timber - namely the Western Hemlock, Burckella, Nyatoh and Malas - in order to provide basic information to construction contractor for them to be able to select and use the appropriate type of timber. The main results of this study are as follows. First, the Western Hemlock had 1.90 $cracks/m^2$, Malas had 0.83 $cracks/m^2$, Burckella had 0.14 $cracks/m^2$, and the Nyatoh had the least number of surface defects at 0.04 $cracks/m^2$. Second, while Malas has the highest degree of strength timber, Nyatoh had the smallest defect rate. This showed that having high timber strength does not necessarily mean it has less defects. Third, the Western Hemlock was the least expensive and Burckella was the most expensive. However, considering the cost of repairing defects, it would be economically advantageous to use Burckella and Nyatoh which have low defect rates. This study aimed to provide basic information to landscape construction contractors for them to be able to select and use the appropriate type of timber when constructing wooden outdoor rest furniture. The results are expected to contribute to quality enhancements and defect reduction in landscape facilities.

• Journal of the Korean Institute of Landscape Architecture
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• v.45 no.6
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• pp.126-136
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• 2017

Gardeners have a desire to not only enjoy a garden but to create the garden themselves and play the role of garden designer. However, they express a great difficulty in gardening activities such as planting and managing plants, the main materials of the garden, due to lack of expertise. Therefore, in order to encourage hobby gardening activities, it is necessary to understand the preferences of the gardeners so that they can easily experience and create a garden, and to provide information on gardening know-how and preferred plants according to places given high preference. This study analyzed the preference for places and plants characteristics according to the purpose of creating a garden. To this end, a questionnaire survey was conducted with visitors to the Korea Landscape and Garden Expo, expected to have been attended primarily by gardening enthusiasts. As a result, gardeners were divided into three groups: appraisal - purposed type, multifunctional type, and participation - purposed type. The group of appraisal purposed type preferred porches and terraces, and private home front yards as well as favored indoor places rather than outdoor places compared with other types. In selecting plants, they prefer plants that can be easily managed in an indoor environment and appreciate natural colors through the colors of flowers and leaves. The multifunctional type group preferred a private home front yard as a garden creation space. Compared with the other types, it showed a high preference for 'unoccupied land in urban housing' and 'community garden' and was analyzed as a group having a high understanding of garden activities. In selecting plants, this group preferred fruit trees or productive plants such as homegrown crops. The participation purposed type group preferred porches and terraces as well as private home front yards as a gardening space. For plants, they preferred plants that are easy to manage. It is meaningful that this study provided preferences for places and plant characteristics according to the creative purpose of the gardener and provided basic data for selecting the place and the plant characteristics of the garden according to the gardener's type.

• Restorative Dentistry and Endodontics
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• v.34 no.6
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• pp.500-507
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• 2009

The purpose of this study is to compare the antibacterial effect of $Listerine^{(R)}$ on two microorganisms (P. gingivalis and E. faecalis) with various root canal irrigants (NaOCl, CHX, EDTA) and to identify possibility of using $Listerine^{(R)}$ as a root canal irrigant. Porphyromonas gingivalis ATCC 3327 and Enterococcus faecalis ATCC 29212 were used in this experiment. For the test irrigants, 0.5%, 1%, 2.5%, 5.25% NaOCl, 0.1%, 0.2%, 1%, 2% CHX, 0.5M EDTA (18.6% EDTA) and $Listerine^{(R)}$ were prepared. Distiled water was used as control. Two methods-1) Comparison of turbidity in broth and 2) Agar diffusion test-were used to determine the extent of antibacterial effect of $Listerine^{(R)}$ and to compare it with that of NaOCl, CHX, and EDTA. All solutions tested were effective against two bacterial strains compared with control (p < 0.001). Any concentration of NaOCl, CHX, and EDTA showed similarly high effectiveness against all bacterial strains. In all experiment, $Listerine^{(R)}$ showed significantly low antibacterial effect compared with the other root canal irrigants (p < 0.05). In conclusion, the results reflect remarkably low antibacterial effect of $Listerine^{(R)}$ as compared with root canal irrigants in general so it is not suitable for the root canal irrigant.

• Journal of the Korean Institute of Traditional Landscape Architecture
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• v.28 no.4
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• pp.85-97
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• 2010

This study analyzed the distribution, structure and environmental condition of the vegetation of the Chionanthus retusus Lindly et Paxton community at the Wansanchielbong in the Jeonju city to offer basic data for sustainable conservation and ecological management system. And the results are as follows; 1. The average pH of soil at the community was pH 5.69 and it was slightly higher than the average of forest soil pH of Korea. But if the degree of pH will be down, it will be needed some more fertilization of Calcium. 2. The total average for contents of organism was 4.98%. And the nitrate - nitrogen content(mg/kg) of A, B, C, D quadrat was 20.29%, 28.87%, 7.65%, and 23.3% respectively. And there were good condition except quadrat C which was contaminated by amount of earth and sand. 3. The flora of the Chionanthus retusus Lindly et Paxton community was listed as 60 taxa; 37 families, 50 genera, 47 species, 10 varieties and 3 forms. The average appearance species of each Quadrat were A sector 30, B sector 26, C sector 19 and D 19 taxa respectively. 4. Surveyed woody plants in the community were as follows : Chionanthus retusus, Zelkova serrata, Quercus variabilis, Cornus walteri, Robinia pseudo-acacia and those were mixed status. And Chionanthus retusus, Zelkova serrata, Robinia pseudo-acacia, Albizzia julibrisin, Cudrania tricuspidata, Symplocos chinensis for. pilosa were mixed in mid layer trees. Herbaceous plants were founded such as Chionanthus retusus, Zelkova serrata, Robinia pseudo-acacia, Grewia parviflora, Rosa multiflora, Trachelospermum asiaticum was dominant with 35~64% in the ground cover, and Commelina communis, Calamagrostis arundinacea, Dryopteris bissetiana, Lilium lancifolium were founded also. 5. The importance values of Chionanthus retusus was 40.2% in the quadrat A1, 50.2% at quadrat A, 50.0% B1, 45.2% B2, 22.4% C1, 73.6% C2, 33.2% D1 and the total average of I.V. was 44.9%. 6. The average height of surveyed Chionanthus retusus was 5.7m and the average DBH was 12.4cm. The number of trees higher than 2m were 107 and the number of trees lower than 2m were 63. The total numbers of Chionanthus retusus were 170. 7. The age of surveyed Chionanthus retusus were analyzed 42 thru 87 years old and that of Zelkova serrata were 42, Quercus variabilis were 60, Quercus aliena were 48, Robinia pseudo-acacia were 40. 8. The number of trees with DBH 40 through 50cm were 6, and that of 30~39cm were 3, and that of 20~29cm were 16, so the total number that was over 20cm was 25. And there were 70 trees under 10cm of DBH and 63 seedlings. It will be very important data to conserve the habitat that the structure and environmental condition of the Chionanthus retusus Lindly et Paxton community at the Wansanchielbong was stable, and sustainable monitoring will be needed. Now that community is nurse forest of Jeonju City but more positive preservation plan will be needed and assigning monument of city or province also be necessary.