• Title/Summary/Keyword: 수조 실험

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Bioaccumulation of Pb and Cd in Blue Mussel (Mytilus edulis) and Oliver Flounder (Paralichthys olivaceus) Exposed to Rearing Media (노출실험을 통한 진주담치(Mytilus edulis) 넙치(Paralichthys olivaceus)의 Pb 및 Cd 축적에 관한 연구)

  • Cho, Yeong-Gil;Kim, Gi-Beum
    • Journal of the Korean Society for Marine Environment & Energy
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    • v.10 no.1
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    • pp.21-28
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    • 2007
  • The bioaccumulation of Pb and Cd dissolved in seawater was assessed measuring the concentrations recorded within blue mussel (Mytilus edulis) and oliver flounder (Paralichthys olivaceus) after two weeks exposure period. The Pb and Cd concentration within the whole body of two testing organisms increased according to the exposure concentrations, and the such tendency was clear specially from the mussel. Maximum metal concentration reached $5,260({\pm}70)\;{\mu}g/g$ for Pb reared under 5.0 mg/L Pb, $1,040({\pm}40)\;{\mu}g/g$ for Cd reared under 1.0 mg/L Cd in the mussel, and indicated that the bioaccumulation of Pb and Cd was directly related to the rearing medium concentrations. Bioconcentration factors (BCF) reached very high values for Pb (maximum value: $12,100{\pm}1,400$) in the mussel reared under lowest Pb concentration (0.01 mg/L). The BCF value for Cd in the mussel were also far higher at exposure to low Cd concentration than high Cd concentration. At higher external concentrations, the BCF for Cd and Pb declined. This demonstrated the ability of two testing organisms to rapidly uptake heavy metals particularly when exposed to low external concentration. The mean Pb concentration was slightly higher in the gill of mussel than in the digestive gland, while Cd showed a higher level in the digestive gland than in the gill.

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PIV Measurements of Wake behind a KRISO 3600TEU Container Ship Model (PIV를 이용한 KRISO 3600TEU 컨테이너선모형선의 반류 측정 및 해석)

  • Sang-Joon Lee;Min-Seok Koh;Choung-Mook Lee
    • Journal of the Society of Naval Architects of Korea
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    • v.39 no.3
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    • pp.48-56
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    • 2002
  • The flow characteristics around KRISO 3600TEU container ship model have been experimentally investigated in a circulating water channel. The instantaneous velocity vectors were measured using 2-frame PIV measurement system. The mean velocity fields and turbulent statistics including turbulent kinetic energy and vorticity were obtained by ensemble-averaging 400 instantaneous velocity fields. The free stream velocity was fixed at 0.6m/s and the corresponding Reynolds number was $9{\times}10^5$. The test sections were divided into two regions, three transverse sections of the wake region(Station -0.5767, -1, -3) and five longitudinal sections of the wake((Z/(B/2)=0, 0.1, 0.2, 0.4, 0.6). In the wake region, large-scale longitudinal vortices of nearly same strength are symmetric with respect to the wake centerline and a relatively weak secondary vortex is formed near the waterline. With going downstream, the strength of longitudinal vortex is decreased and the wake region expands.

A Numerical Modeling of Surcharged Manhole Flow with the Consideration of the Energy Loss Coefficient (과부하 맨홀의 손실계수를 고려한 흐름의 수치모형)

  • Kim, Kyoung Beom;Kim, Jung Soo;Yoon, Sei Eui
    • KSCE Journal of Civil and Environmental Engineering Research
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    • v.33 no.2
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    • pp.521-528
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    • 2013
  • Urban drainage systems are generally designed as open channel flow. The system, however, shows a partially surcharged flow in its body, especially at junctions or manholes. Thus, a special case of this condition needs to be explained as pressurized flow condition for designing the sewer system. This study considered the surcharged manhole flows during an unexpected rainfall event or an excess of design frequency. Overflows from surcharged manholes and urban flooding can occur from the effect of surcharged flows. Thus, sewer systems should be designed with the concept of open channel flow and pressurized flow. Also, energy losses in a manhole need to be considered. The aim of this study is to develop the numerical model which can evaluate the effect of the energy losses at the manhole. The numerical model was verified and compared with hydraulic model and SWMM. The results showed that the water depth of numerical model was in good agreement with hydraulic model at the each manhole. However, the SWMM underestimated the water depth because that model ignored the energy losses at manholes. Thus, the developed numerical model in this study could be a useful tool for the assessment of a conveyance of urban drainage system.

Effects of Dietary Composition, Feeding Satiation Rate and Feeding Frequency of Extruded Pellets on Growth and Body Composition of Flounder Paralichthys olivaceus (부상사료의 사료조성, 공급율 및 공급횟수가 넙치의 성장 및 체조성에 미치는 영향)

  • Seo, Joo-Young;Jang, Hyun-Seok;Kim, Kyoung-Duck;Kim, Gun-Up;Lee, Sang-Min
    • Journal of Aquaculture
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    • v.18 no.2
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    • pp.98-106
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    • 2005
  • A feeding trial of two extruded pellets (EPI, 45% crude protein with 7% crude lipid and EP2, 47% crude protein with 10% crude lipid)${\times}$ two satiation rates (satiation and 80% satiation)${\times}$ three feeding frequencies (three, two and one meal a day) factorial design with three replications was carried out to investigate the optimum feeding frequency and satiation rate for growth of juvenile flounder. Flounder averaging 55 g were fed a extruded pellets for 58 days. Daily feed intake was affected by dietary composition, feeding frequency and satiation rate (P<0.05). Weight gain of fish fed the EP1 and EP2 was not affected by both dietary composition and feeding frequency (P>0.05), but affected by feeding satiation rate (P<0.05). Weight gain of fish fed the each of EP1 and EP2 twice daily with satiation was the highest among treatments, but was not significantly different to that of fish fed the diets three times daily with satiation feeding. Feed efficiency and protein efficiency ratio of the fish were not affected by dietary composition, feeding frequency and satiation rate (P>0.05). Moisture, crude protein and crude lipid contents of the muscle of fish were affected by satiation rate. Moisture and crude protein contents of the liver were affected by dietary composition. Based on fish performance results, it can be concluded that the proper feeding frequency for the growth of juvenile flounder grown from 55 g to 90 g is two meals a day with satiation feeding, regardless of dietary composition used in this study.

Numerical study on the thermal-hydraulic safety of the fuel assembly in the Mast assembly (수치해석을 이용한 마스트집합체 내 핵연료 집합체의 열수력적 안전성 연구)

  • Kim, YoungSoo;Yun, ByongJo;Kim, HuiYung;Jeon, JaeYeong
    • Journal of Energy Engineering
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    • v.24 no.1
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    • pp.149-163
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    • 2015
  • In this study, we conducted study on the confirmation of thermal-hydraulic safety for Mast assembly with Computational Fluid Dynamics(CFD) analysis. Before performing the natural convection analysis for the Mast assembly by using CFD code, we validated the CFD code against two benchmark natural convection data for the evaluation of turbulence models and confirmation of its applicability to the natural convection flow. From the first benchmark test which was performed by Betts et al. in the simple rectangular channel, we selected standard k-omega turbulence model for natural convection. And then, calculation performance of CFD code was also investigated in the sub-channel of rod bundle by comparing with PNL(Pacific Northwest Laboratory) experimental data and prediction results by MATRA and Fluent 12.0 which were performed by Kwon et al.. Finally, we performed main natural convection analysis for fuel assembly inside the Mast assembly by using validated turbulence model. From the calculation, we observed stable natural circulation flow between the mast assembly and pool side and evaluated the thermal-hydraulic safety by calculating the departure from nucleate boiling ratio.

Ultrastructural Study of the Effect of Activated Carbon Treatment on the Mouse Kidney Treated with Lead (납 투여된 마우스의 신장에서 활성탄 처리 효과에 대한 전자현미경적 연구)

  • Chung, Min-Ju;Yoon, Jung-Sik;Chung, Kyung-A;Kim, Young-Ho;Roh, Young-Bok
    • Applied Microscopy
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    • v.29 no.2
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    • pp.149-162
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    • 1999
  • For investigation of the activated carbon on the mouse kidney treated with Pb, the activated carbon (40 mg/kg) and Pb (30 mg/kg) were treated orally for three and six weeks, respectivelly, and observed by the electron microscope. On the glomerulus of the group with only Pb, the basal membrane thicked, projected, and the foot processes fused. On the proximal convoluted tubules, the number of microvilli were decreased and the number of vacuoles and lysosome increased on the cytoplasm. The mitochondria and endoplasmic reticulum were extended and ribosomes dropped from the ER. On the giomerulus of the group with Pb-activated carbon, the basal membrane and the foot processes were merely changed. On the proximal convoluted tubules, the shapes and number of microvilli were not changed and the number of vacuoles, microbodies, and lysosomes decreased. The shapes of mitochondria and endoplasmic reticulum observed almost similar with control group. Th at is, elongated mitochondria and attached ribosomes to endoplasmic reticulum. As result, the activated carbon has positive effect on reducing toxicity of lead in the mouse kidney in the view of electron microscope.

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Effect on the Wake Flow according to Various length of Rectangular Cylinder in a Parallel Arrangement (병렬구조를 가진 장방형 실린더의 길이가 후류 유동에 미치는 영향)

  • Choe, Sang-Bom;Cho, Dae-Hwan
    • Journal of the Korean Society of Marine Environment & Safety
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    • v.20 no.6
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    • pp.760-767
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    • 2014
  • An experimental study is carried out to investigate the effect of jet stream in the gab of rectangular cylinders with different length in a parallel arrangement by using PIV method in a circulating water channel. The height(h) of the rectangular cylinder and the gap between the cylinder is 10mm, and the width(B) which is 300mm. The length of the model for flow direction was applied to 30mm, 60mm, 90mm & 120mm, The aspect ratio of a model on the basis of height(H=30mm) is 1, 2, 3 and 4. Reynolds number $Re=1.4{\times}10^4$, $Re=2.0{\times}10^4$, $Re=2.9{\times}10^4$ based on the height(H) of model for the distance of tidal distributions as of water depth have been applied during the whole experiments. The measurement area was set to 5H rear of the cylinder. As a result, Vortex size in the wake area were increased as velocity increased. and high aspect ratio increased through-flow velocity component in the near wake. Velocity deficit increased highly after near-wake area and low aspect ratio.

Effects of Different Light Wavelengths on the Growth of Olive Flounder (Paralichthys olivaceus) (빛의 파장이 넙치 Paralichthys olivaceus의 성장에 미치는 영향)

  • Benedict, Ndada Regina;Kim, Yeo-Reum;Kim, Jong-Myoung
    • Journal of Life Science
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    • v.29 no.3
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    • pp.311-317
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    • 2019
  • To investigate the effects of light on growth in fish, olive flounder (Paralichthys olivaceus) were reared under four kinds of monochromatic light-emitting diodes (LEDs) at violet (400 nm), blue (465 nm), green (508 nm), and red (635 nm) wavelengths, along with a white fluorescent lamp as control. The rearing experiments were carried out with 15 fish per tank under different wavelength illumination at the same intensity. After rearing the fish under a 12 hr:12 hr light:dark photoperiod for 60 days, percentage increases in weight gain of $269.92{\pm}13.02$, $363.21{\pm}3.74$, $433.22{\pm}4.83$, $290.17{\pm}11.83$, and $340.74{\pm}26.58%$ and increases in specific growth rates (SGR) of $2.18{\pm}0.06$, $2.56{\pm}0.07$, $2.79{\pm}0.01$, $2.27{\pm}0.05$, and $2.47{\pm}0.10$ were observed in fish grown under the illumination of red, blue, green, and violet LEDs and the white fluorescent light, respectively. The results show faster growth in fish reared under green LEDs, but slower growth in those reared under red light. Differences in most blood parameters were minor, aside from an increased level of glutamic oxaloacetic transaminase in the fish grown under red LED illumination. Histological analysis of the retina showed few changes in the ratio of photoreceptor layer thickness to total retina thickness in fish reared under the green LEDs compared to those in other illumination groups. These results indicate that green LED light can foster increased growth in olive flounder with no distinct harmful effects on their light-sensitive photoreceptor layers.

Image enhancement in ultrasound passive cavitation imaging using centroid and flatness of received channel data (수신 채널 신호의 무게중심과 평탄도를 이용한 초음파 수동 공동 영상의 화질 개선)

  • Jeong, Mok Kun;Kwon, Sung Jae;Choi, Min Joo
    • The Journal of the Acoustical Society of Korea
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    • v.38 no.4
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    • pp.450-458
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    • 2019
  • Passive cavitation imaging method is used to observe the ultrasonic waves generated when a group of bubbles collapses. A problem with passive cavitation imaging is a low resolution and large side lobe levels. Since ultrasound signals generated by passive cavitation take the form of a pulse, the amplitude distribution of signals received across the receive channels varies depending on the direction of incidence. Both the centroid and flatness were calculated to determine weights at imaging points in order to discriminate between the main and side lobe signals from the signal amplitude distribution of the received channel data and to reduce the side lobe levels. The centroid quantifies how the channel data are distributed across the receive channel, and the flatness measures the variance of the channel data. We applied the centroid weight and the flatness to the passive cavitation image constructed using the delay-and-sum focusing and minimum variance beamforming methods to improve the image quality. Using computer simulation and experiment, we show that the application of weighting in delay-and-sum and minimum variance beamforming reduces side lobe levels.

Critical Low Temperature and Response of Behavioral Tolerance in Red Seabream Pagrus major fingerlings Exposed to Cold Shock (저온 충격에 노출된 참돔 Pagrus major 치어의 임계 저 수온 및 행동 내성 반응)

  • Yoon, Sung Jin
    • Journal of the Korea Academia-Industrial cooperation Society
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    • v.22 no.1
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    • pp.575-584
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    • 2021
  • The critical low temperature and response of the behavioral tolerance of red sea bream Pagrus major fingerlings were determined using the continuous behavior monitoring system (CBMS). The behavior of the experimental organisms was observed by decreasing the water temperature by 2.0℃ and 4.0℃ every 12 hours and 24 hours in the range of 8.0-20.0℃. An unstable behavior pattern was observed in red seabream fingerlings exposed to water temperatures below 12.0℃, in which the swimming activity decreased and repeatedly stopped, regardless of the exposure time and water temperature fluctuation. The swimming ability of the organisms exposed to 8.0-10.0℃ decreased sharply, and the behavior of staying at the bottom of the test tank was observed. Only 50 % of the organisms survived due to the low-temperature stress, and all individuals died within six hours after the cold shock. In addition, the behavior index (BI) decreased rapidly, and the amplitude change of the coefficient of variation (CV) was found to have a greater variation than the other water temperatures (p<0.05). Low-temperature stress of red sea bream is promoted at 12.0℃, and it is interpreted as the tolerance limit, which can induce a sublethal response of the organisms exposed to cold shock of 8.0-10.0℃.