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A Structural Framework on Psychological Adaptation and Sequential Changes during the COVID-19 Pandemic (코로나19 팬데믹에 대한 심리적 적응과 연쇄적 변화의 구조 모형)

  • Ko, Dong-Woo;Seo, Hyun-Sook
    • Korean Journal of Culture and Social Issue
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    • v.27 no.4
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    • pp.351-389
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    • 2021
  • This qualitative study aimed to develop a structural framework that explains the process of psychological adaptation and sequential changes being perceived by Korean people under the COVID-19 Pandemic past year. Setting a tentative analysis frame induced from antecedent literatures about psychological phenomena during the COVID-19 pandemic, the qualitative data were collected from 6 Korean adults by semi structured individual interviews. For the data, content analysis applied from the grounded theory were performed. As a result, the initial framework was extended and revised to describe the psychological phenomena under the pandemic. This paradigm structure includes the process of 'causal factors ⇒ psychological main phenomena ⇒ sequential results' being intervened by personal contextual situations and psychological characteristics, as moderators. The category of causal factors were the COVID-19 pandemic, relevant critical incidents, and social distancing policy. The main phenomena reflected either positive, negative, or complicated experiences. The sequential psychological results included transformation of cognitive system or behavior patterns. Various variables such as psychological sense of community and social responsibility, psychological capability for leisure, and positive psychological capital were found out as moderating factors. In discussion and conclusion, theoretical/practical implications of the results and direction to study in the future were suggested.

The Interference of Organic Matter in the Characterization of Aquifers Contaminated with LNAPLs by Partitioning Tracer Method (LNAPLs 오염 지반에 분배성 추적자 시험법 적용 시 유기물질의 영향에 관한 연구)

  • Khan, Sherin Momand;Rhee, Sung-Su;Park, Jun-Boum
    • Journal of the Korean Geotechnical Society
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    • v.24 no.9
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    • pp.13-21
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    • 2008
  • Partitioning tracer method is a useful tool to characterize large domains of the aquifers contaminated with light nonaqueous phase liquids (LNAPLs). Sorption of the partitioning tracers to the organic matter content of soil can potentially influence the efficacy of partitioning tracer method. LNAPL-water partitioning coefficients of tracers ($K_{nw}$), measured by static method, showed linear relationship. Sorption isotherm tests were conducted to evaluate the sorption capacity of the soils packed in the columns and the results were appropriately represented by Freundlich sorption isotherm. The sorption of tracers proportionally increased with the increase of the organic matter content of the soil. Laboratory experiments were conducted in four columns each packed with soils of different organic matter contents to determine the potential interference effects of sorption to soil organic matter content and correction factors for the errors in estimation of LNAPLs by partitioning tracer method. Though there were no contaminants added, breakthrough curves from columns packed with mixture of Jumunjin standard sand and organic matter showed separation of tracers. Columns were then contaminated to residual saturation with kerosene and breakthrough curves were obtained. The results show that sorption of tracers to soil organic matter leads to an increase in the retardation factor (R) and hence, to an overestimation of the saturation of LNAPLs. A relation between the percentage of organic matter content and the corresponding percentage error in the estimation of NAPLs has been developed.

Production of Transgenic Murine Embryos using Haploid Spermatids Transfected with EGFP Gene (EGFP 유전자가 도입된 반수체 정자세포에 의한 형질전환 설치류 난자의 생산)

  • Kang, K.Y.;Song, S.J.;Lee, H.T.;Chung, K.S.
    • Korean Journal of Animal Reproduction
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    • v.25 no.4
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    • pp.305-315
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    • 2001
  • In this study, the production of transgenic embryo was attempted by microinjection or round spermatid cultured with foreign DNA. At first, the expression of haploid spermatids specific gene, mTP1 in mouse and hPrm2 in hamster spermatids were investigated by RT-PCR method in testes of young mice and hamster testis. The specific gene expression first appeared at 18 days post partum (dpp) in mice spermatid and 20 dpp in hamster spermatid. Therefore, the round spermatids isolated from 17 dpp mice and 19 dpp hamster were used for the introduction of foreign EGFP gene into haploid round spermatids. For the introduction of EGFP gene haploid round spermatids suspended in medium including EGFP gene were treated with a different electric field strength at 0.11, 0.18 and 0.44 ㎸/cm. After electrical stimulation, viability of testicular sperm cells and 67.6%, 66.4% and 49.9%, in mice and 62.6%, 57.9% and 27% in hamster, respectively. These values were significantly lower than those of non-treated control groups 80.5% in mouse and 69.1% in hamster After 72 hrs culture, the highest expression rate of EGFP gene, 28.5% in mice and 32.1% in hamster were obtained from tile spermatogenic cells electroporated by the field strength or 0.18 ㎸/cm. Then, the ability of fertilization and embryonic development of haploid spermatids transfected with foreign EGFP gene were estimated by the microinjection of spermatids into hamster oocytes. The Irate pronuclear formation rate (77.5%) was lower than non-treated control (80%), and the cleavage rate of the treated group (58.8%) was lower than control (65%). To prove the foreign EGFP integration in hamster embryos, 2-cell stage hamster embryos were subjected to the observation under the fluorescence microscope, and the PCR analysis. As a result, about 44% of 2-cell embryos were showed the integration of EFGP gene into their genome. Therefore, These results suggest the possibility to produce transgenic hamsters by microinjection of haploid spermatid transfected with foreign DNA.

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Application of Multi-Dimensional Flood Damage Analysis for Urban Flood Damage (다차원 홍수피해산정방법을 이용한 도시지역의 홍수피해액 산정)

  • Lee, Keon Haeng;Choi, Seung An;Kim, Hung Soo;Shim, Myung Pil
    • KSCE Journal of Civil and Environmental Engineering Research
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    • v.26 no.4B
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    • pp.363-369
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    • 2006
  • A simple and an improved methods for the economic analysis of the flood control project has been in previous studies in Korea. In 2004, the Multi-Dimensional Flood Damage Analysis (MD-FDA) was developed and now it is widely used for the economic analysis of flood control project. However, the MD-FDA was developed for general damage assessment and analysis without consideration of specific regional characteristics such as urban and rural areas. To compensate the MD-FDA for the application in urban area, a part of damage estimation components is modified and a component for the flood damage estimation is suggested. The component we suggest is for the consideration of the capability of stormwater pump stations in the study area. When flood is occurred in the urban area, the damage potential is larger than the rural area because of the concentration of human lives and properties. So, many stormwater pump stations are located in the urban area and the inundation depth is estimated by considering the capabilities of pump stations. We also compensate the damage components such as the damages of industrial area, and public facilities for the flood damage estimation of the urban area. The results by the compensated MD-FDA for the urban area application with those by original MD-FDA are compared. As a result the B/C ratio showed 6.75 and 5.51 respectively for the modified and original MD-FDA. This difference might be largely affected by the damage rate of the public facilities.

Influence of Video Clip-based Pedagogical Reasoning Activity on Elementary Preservice Teachers' Science Lesson Planning (비디오 클립을 활용한 교육적 추론 활동이 초등 예비교사의 과학 수업 계획에 미치는 영향)

  • Song, Nayoon;Yoon, Hye-Gyoung
    • Journal of Korean Elementary Science Education
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    • v.43 no.1
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    • pp.170-184
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    • 2024
  • This study focused on the practical research needed to improve elementary school science lesson plans. Specifically, a video clip-based pedagogical reasoning activity that included elementary student misconceptions was presented and the influences of this activity on preservice teachers' science lesson planning were assessed. First, the eight preservice teacher participants were asked to write a lesson plan for a dissolution and solution unit, after which a first semi-structured interview was conducted. Then, the participants participated in a video clip-based pedagogical reasoning activity. Based on the activity results, the participants revised their previously planned lessons, and second semi-structured interviews were conducted. The data from the preservice teachers' lesson plans and interview transcripts were analyzed using a constant comparative method to investigate the lesson plan changes. It was found that after the video clip-based pedagogical reasoning activity, the preservice teacher tightened the activity or changed the material to understand the students' thinking processes. In addition, they supplemented their goals and assessment criteria to accommodate the diverse students' thinking. Some also specified motivational strategies that considered student interests, motivation, and possible misconceptions. However, some preservice teachers still set goals that did not sufficiently account for student misconceptions and some planned the student assessments based only on the learning goals rather than the students' thinking. The few preservice teachers were able to develop motivational strategies that considered interest, motivation, and misconceptions. The preservice teachers claimed that they had difficulty predicting the misconceptions and connecting these to the lesson content. Discussions were then held to assist the preservice teachers to consider possible student misconceptions when planning their lessons.

Practical Applications of DNA Marker-Assisted Selection and OPU-Derived IVF Embryo Transfer for the Production of High Quality Meat in Hanwoo II. Production of IVF Embryos Derived Transvaginal Ovum Pick-up from DNA Marker-Proved Hanwoo (유전자 분석을 통하여 선발된 한우로부터 초음파 유래 체외수정란 이식에 의한 고품질 한우 생산기술의 실용화 II. DNA 검정우로부터 초음파 유래 체외수정란의 생산에 관한 연구)

  • 박희성;이지삼;진동인;박준규;홍승표;이명열;정장용
    • Journal of Embryo Transfer
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    • v.16 no.3
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    • pp.193-201
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    • 2001
  • This study was designed to examine the factors affecting in fertilization and development of embryos in vitro, and to examine whether zone drilling by laser irradiation can improve the hatching rate of IVF embryos from DNA marker-proved Hanwoo. DNA markers related to marbling score were identified using DNA fingerprinting with Ml3 probe and restriction enzyme Hae III. Oocytes were aspirated from immature ovarian follicles using a combined method of rectal ovarian-palpation and transvaginal ultrasound-guidance(6.5MHz) under local anesthesia. The aspirated oocytes were washed twice with fresh D-PBS containing 5% FBS and were rewashed 4 to 5 times with TCM-199 containing 5% FBS. A morphological grade of I to IV was assigned to each oocyte. Data were analyzed using the GLM procedure of SAS. Sperm separation methods did not have any significant effect on cleavage or developmental abilities of IVF embryos. Significantly(P<0.05) higher cleavage rate was observed in embryos from GI(60.0%, 3/5), GII(69.2%, 18/26) and GIII(62.1%, 59/95) compared to embryos from GIV oocytes(36.2%, 25/69). And the developmental rate to blastocyst stage was higher(P<0.05) in embryos from GI(33.3%, 1/3) and GII oocytes(38.9%, 7/18) than those from GIII(16.9%,10/59) and GIV oocytes(4.0%, 1/25). There was no significant difference in development of IVF embryos to blastocyst by media for in vitro culture. Proportion of hatched blastocyst was significantly(P<0.05) higher in embryos received zona drilling by laser than those of non-drilled.

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A Comparative Study of Internet Addiction among Middle and High School Students in Seoul, Cheonan, and Rural Area (서울, 천안, 농촌지역 중 고교 학생들의 인터넷 중독에 대한 비교 연구)

  • Lee, Seok-Bum;Lee, Kyung-Kyu;Paik, Ki-Chung;Kim, Hyun-Woo
    • Korean Journal of Psychosomatic Medicine
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    • v.12 no.2
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    • pp.145-156
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    • 2004
  • Objectives : Internet addiction is a newly appeared addictive phenomenon that is defined as 'difficulty in real life due to internet over-use', 'excessive time spent on the internet or replacement of genuine real relationships with superficial virtual ones' or 'weakness in self-control about internet use and if discontinued, fallen in psychological confusion and anergic state'. This study was aimed to compare the internet addiction rate, demographic factors, computer using patterns, anxiety, depression, and internet self-efficacy and outcome expectancy between Korean middle and high school students in three areas - Seoul, Cheonan, and Rural areas - that were different in economic, cultural, and geographic state. Methods : Subjects are consisted of middle and high school students in Seoul, Chunan, and Rural areas(N=1718). Self-rating questionnaire included demographic data, Korean Spielberger State-Trait Anxiety Inventory, Korean Self-Rating Depression Scale(SDS), questions for internet-using pattern, Korean version of Internet Addiction Scale invented by K. S. Young, Internet Self-efficacy and Out-come Expectancy Scale. Results: In this study, prevalence of internet addiction was 2.9%. There was no significant difference found in prevalence of internet addiction among three areas. In all subjects, there were significant differences in anxiety, depression, internet self-efficacy and outcome expectancy, leisure style, places of internet use, internet connecting method, and insight on internee addiction. But, in addicted group, there was no significant difference among three areas. Conclusion: This study suggested that the difference in economic, cultural, and geographic state was not related to prevalence of internet addiction. Although there were significant differences in anxiety, depression, self-efficacy associated with internee use, leisure style, places of internet use, internet connecting method, insight on internet addiction among three areas, there were no significant difference in addicted group among three areas. So, we concluded that the difference in economic, cultural, and geographic state did not influence the prevalence of internet addiction, and despite the difference in economic, cultural, and geographic state, people in three areas were equally influenced by internet addiction.

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A Retrospective Study on the Risk Factors and the Effect of Higher Somatic Cell Count in Milk on Reproductive Performance in Dairy Cows (젖소에서 비유초기 체세포 증가 위험 요인 및 번식효율에 미치는 영향 분석 연구)

  • Seo, Bo-Sung;Shin, Eun-Kyung;Jeong, Jae-Kwan;Kang, Hyun-Gu;Kim, Ill-Hwa
    • Journal of Veterinary Clinics
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    • v.31 no.4
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    • pp.272-277
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    • 2014
  • This retrospective study evaluated the effect of somatic cell count (SCC) in milk during early lactation on reproductive performance in dairy cows. Data were collected on 774 cows from six dairy farms, including cow parity, dates of previous calving, artificial insemination, pregnancy diagnosis, incidence of postpartum endometritis, reproductive performance (the intervals from calving to first insemination and conception), milk production and SCC. Data on 774 lactations were grouped based on the average first 3 months postpartum linear somatic cell score (SCS) as T1 (< 3.0, n = 521), T2 (3.0 ${\leq}$ and < 4.0, n = 113), and T3 (${\geq}$ 4.0, n = 140) groups. The odds ratio (OR) for the probability of endometritis increased 1.6 (p < 0.05) and 3.2 times (p < 0.0001) in the T2 and T3 groups, respectively, compared with that in the T1 group. The hazard of first insemination by 150 days in milk (DIM) was lower in the T3 group (hazard ratio [HR]: 0.76, p < 0.01) than in the T1 group. First insemination conception rate did not differ among the 3 groups (28.7-34.2%, p > 0.05). The hazard of pregnancy by 365 DIM in the T3 group was lower (HR: 0.75, p < 0.05 respectively) than in the T1 and T2 groups. The SCS during 4 to 7 months postpartum differed (p < 0.0001) among the 3 groups. Farm and cow parity were important risk factors for higher SCS (${\geq}$ 4.0). Multiparous cows were more likely to have a higher SCS (OR: 2.26, p = 0.0005) compared with primiparous cows. In conclusion, higher SCS (${\geq}$ 4.0) during early lactation was associated with decreased reproductive performance of dairy cows.

Effects of Zardaverine in Freezing Extender on Kinetic Characteristics of Post-Thawed Boar Sperm (동결보존액에 Zardaverine의 첨가가 동결-융해 후 돼지 정자의 운동학적 특성에 미치는 영향)

  • Kim, Jeong A;Cho, Eun Seok;Jeong, Yong Dae;Choi, Yo Han;Hong, Jun Ki;Kim, Young Sin;Chung, Hak Jae;Baek, Sun Young;Sa, Soo Jin
    • Journal of the Korea Academia-Industrial cooperation Society
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    • v.21 no.9
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    • pp.251-258
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    • 2020
  • This study investigated the effect of Zardaverine supplementation in freezing extender, on kinetic characteristics of post-thawed boar sperm. Cryopreservation of boar sperm is an important technique of assisted reproductive technology and genetic resource banking. Although this technique is particularly useful, freeze-thaw cycles associated with sperm cryopreservation significantly reduce sperm quality. Semen from mature Duroc boars were collected and cryopreserved in freezing extenders (LEY) treated with varying concentrations of Zardaverine (0, 20, 50, 75, 100 𝜇M). The time-dependent kinetic characteristics of post-thawed spermatozoa were determined after thawing by applying computer-assisted sperm analysis (CASA). We observed that the motility immediately after thawing was significantly higher in 20 𝜇M stocks than in control (0 𝜇M) and the other treatments (p<0.05). Curvilinear velocity (VCL) in 0 𝜇M and 20 𝜇M stocks were significantly higher than the other treatment groups, except 75 𝜇M (p<0.05). Higher average path velocity (VAP) was obtained at 20 𝜇M as compared to 100 𝜇M, whereas amplitude of head lateral displacement (ALH) was significantly higher at 20 𝜇M than 50 𝜇M and 100 𝜇M (p<0.05). No differences were obtained for Straight-line velocity (VSL) and Linearity (LIN). In conclusion, our results indicate that Zardaverine improves the motility, VCL, VAP, and ALH of post-thawed boar sperm.

Changes of Frozen-Thawed Semen Characteristics in Miniature Pig and Duroc (Miniature Pig와 Duroc 종간의 동결-융해 후 정액 성상 비교)

  • Lee, Y.S.;Choi, W.C.;Lee, S.H.;Cheong, H.T.;Lee, S.Y.;Yang, B.K.;Park, C.K.
    • Journal of Embryo Transfer
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    • v.21 no.3
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    • pp.263-271
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    • 2006
  • The purpose of this study was undertaken to compare ability of frozen-thawed sperm characteristics between two strains (miniature pig and Duroc). The semen was collected by gloved-hand method into a pre-warmed ($37^{\circ}C$) thermos bottle. The semen was diluted with same volume extender and added to LEY solution for freezing. The diluted semen was placed in 0.5 ml straws, and freezing was initiated by exposing the straws to liquid nitrogen ($LN_2$) vapours for 10 min before placing them into $LN_2$ for cryopreservation. The frozen-semen straw were thawed at 20, 37 and $50^{\circ}C$ for 1 min, 45 sec and 10 sec within water-bath. The semen sample were evaluated at 0, 3, 6, 9, and 12 h after incubation at $37^{\circ}C$ for analysis of sperm ability. Abnormality of spermatozoa in miniature pig was significantly (p<0.05) higher than that in Duroc at 0, 9 and 12 h of post-thawing incubation after frozen-thawing. The percentage of F-patterned spermatozoa in miniature pig was significantly (p<0.05) lower, while the percentage of AR (acrosome reacted spermatozoa) pattern was higher in the miniature than in the Duroc. On the other hand, there was no significant difference in the viability of spermatozoa thawed at different temperature ($20^{\circ}C\;and\;37^{\circ}C$) between two species, but the viability in miniature pig was higher (p<0.05) than in Duroc when sperm was thawed at $50^{\circ}C$. In conclusion, this study suggest that suitable freezing method for miniature pig semen is required for increasing post-thawing viability and fertilization capacity.