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http://dx.doi.org/10.5762/KAIS.2020.21.9.251

Effects of Zardaverine in Freezing Extender on Kinetic Characteristics of Post-Thawed Boar Sperm  

Kim, Jeong A (Swine Science Division, National Institute of Animal Science, Rural Development Administration)
Cho, Eun Seok (Swine Science Division, National Institute of Animal Science, Rural Development Administration)
Jeong, Yong Dae (Swine Science Division, National Institute of Animal Science, Rural Development Administration)
Choi, Yo Han (Swine Science Division, National Institute of Animal Science, Rural Development Administration)
Hong, Jun Ki (Swine Science Division, National Institute of Animal Science, Rural Development Administration)
Kim, Young Sin (Swine Science Division, National Institute of Animal Science, Rural Development Administration)
Chung, Hak Jae (Swine Science Division, National Institute of Animal Science, Rural Development Administration)
Baek, Sun Young (Swine Science Division, National Institute of Animal Science, Rural Development Administration)
Sa, Soo Jin (Swine Science Division, National Institute of Animal Science, Rural Development Administration)
Publication Information
Journal of the Korea Academia-Industrial cooperation Society / v.21, no.9, 2020 , pp. 251-258 More about this Journal
Abstract
This study investigated the effect of Zardaverine supplementation in freezing extender, on kinetic characteristics of post-thawed boar sperm. Cryopreservation of boar sperm is an important technique of assisted reproductive technology and genetic resource banking. Although this technique is particularly useful, freeze-thaw cycles associated with sperm cryopreservation significantly reduce sperm quality. Semen from mature Duroc boars were collected and cryopreserved in freezing extenders (LEY) treated with varying concentrations of Zardaverine (0, 20, 50, 75, 100 𝜇M). The time-dependent kinetic characteristics of post-thawed spermatozoa were determined after thawing by applying computer-assisted sperm analysis (CASA). We observed that the motility immediately after thawing was significantly higher in 20 𝜇M stocks than in control (0 𝜇M) and the other treatments (p<0.05). Curvilinear velocity (VCL) in 0 𝜇M and 20 𝜇M stocks were significantly higher than the other treatment groups, except 75 𝜇M (p<0.05). Higher average path velocity (VAP) was obtained at 20 𝜇M as compared to 100 𝜇M, whereas amplitude of head lateral displacement (ALH) was significantly higher at 20 𝜇M than 50 𝜇M and 100 𝜇M (p<0.05). No differences were obtained for Straight-line velocity (VSL) and Linearity (LIN). In conclusion, our results indicate that Zardaverine improves the motility, VCL, VAP, and ALH of post-thawed boar sperm.
Keywords
Boar; Spermatozoa; Frozen semen; Phosphodiesterase inhibitor; Zardaverine;
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