• Title/Summary/Keyword: 수용성추출

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The Effect of Ganoderma lucidum Water Soluble Extract on Higher Alcohol Production of Saccharomyces cerevisiae (영지(靈芝)의 수용성(水瀉性) 추출물(抽出物)이 Saccharomyces cerevisiae의 고급 Alcohol 생성(生成)에 미치는 영향(影響))

  • Joo, Hyun-Kyu;Lee, Joong-Keun
    • Korean Journal of Food Science and Technology
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    • v.20 no.1
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    • pp.52-58
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    • 1988
  • This study has investigated the effect of Ganoderma lucidum water soluble extract on higher alcohol production of Saccharomyces cerevisiae. S. cerevisiae was inoculated in malt wort media which were added 0, 0.01, 0.1, 0.5 or 1.0% extracts of G. lucidum and fermented at $15^{\circ}C$ for 120 hours respectively. Some results about cell growth, changes of pH and sugarity and alcohol composition during fermentation are as follows; The cell growth of S. cerevisiae was remarkablely increased as G. lucidum extract was added into each step. It was increased to 1.2, 1.5, 1.9 times on added group of 0.1%, 0.5%, 1.0% G. lucidum extract than control group after the fermentation of 120 hours. Content of sugar was systematically low on step of addition of G. lucidum extract after the fermentation of 120 hours but pH was systematically high. The more the quantity of G. lucidum extract was added, the more total alcohol quantity were produced during fermentation. Especially higher alcohol content was more increased about 1.8 times on 1.0% group (219.9 ppm w/v) than on control group (117.26 ppm w/v) after the fermentation of 120 hours.

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Separation and Identification of Antimicrobial Substances from Prunus mume extract (매실추출물로부터 항균물질의 분리 및 구조동정)

  • Park, Woo-Po;Lee, Seung-Cheol;Kim, Sung-Yong;Choi, Sung-Gil;Heo, Ho-Jin;Cho, Sung-Hwan
    • Food Science and Preservation
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    • v.15 no.6
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    • pp.878-883
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    • 2008
  • Prunus mume was extracted using various solvents including ethyl ether, ethyl acetate and n-butanol. The extracts were fractionated by column chromatography. Antimicrobial compounds (A, B and C) in fractions showing antimicrobial activity were purified by Sephadex LH 20 column chromatography, and identified by $^{1}H$- and $^{13}C$-NMR analysis as isoeugenol, nomilin and $\beta$-sitosterol, respectively.

레이다와 전파신호처리 기술(I)

  • 곽영길
    • The Proceeding of the Korean Institute of Electromagnetic Engineering and Science
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    • v.5 no.1
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    • pp.100-110
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    • 1994
  • 레이다 신호는 대표적인 전자파 신호로서 주변환경에 따라 시간, 주파수, 공간 영역에서 고유한 신호특성을 가지고 있으며, 신호처리 기법도 다양하다. 본 논문에서는 먼저 레이다를 위한 전파 신호처리 의정의와 필요성을 언급한뒤, 레이다 신호환경 특성을 살펴보고 신호처리를 위한 신호의 시간 및 스펙트럼 특성에 대해 기술하였다. 그리고, 신호특성에 적합한 신호처리기의 구현을 위해 레이다 신호처리에 관 련된 주요 기법에 대해 개괄적으로 설명하였다. 레이다 신호처리 분야는 일반적으로 잘 알려진 음성이 나 영상신호처리 분야와 달리 고유한 알고리듬과 구조가 요구된다. 신호처리기법으로서 레이다 파형설 계, 해상도 모호성, 펄스압축, 클러터제거, 도플러처리, 일정오경보탐지, 클러터 지도, 표적군 형성/ 추출, 표적식별, 레이다영상기법, 적응배열처리 등에 관해 개괄적으로 설명하였다. 레이다 선호처리 기술은 "스마트"한 레이다를 위한 두뇌 역할을 하기때문에 그 필요성과 중요성이 증가하고 있다. 그러나, 고속, 대용량의 신호를 주어진 빔 주사시간동안에 실시간으로 처리하여 표적 정보를 추출해야 하기 때문에 아직도 상용 프로세서의 속도 한계내에서 알고리듬의 수행에 다소 제약을 받고 있으나, 최근 디지탈 신호처리 전용의 고속 칩의 출현으로 많은 발전을 가져오고 있다. 끝으로, 향후 레이다 신호처리 발전 추세와 응용분야에 대해 살펴보았다. 응용분야는 군수 및 민수용의 겸용 파급효과가 매우 크고, 군용의 대공탐색 및 조기경보, 전장감시뿐만 아니라 전투기 탑재용으로 필수적이며, 특히 민수용의 공 항, 항공기, 선박, 위성 등 매우 다양하다. 최근 발전추세에 따른 기술로서 다중모드 신호처리, 고집적 회로기술, 적응배열, 디지탈 빔형성, 적응성, 고분해능 및 방향성, 표적식별, 다차원 신호처리에 대해 언급 하였다.

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Cyanex 301 추출제에 의한 Am(III)과 Eu(III)의 상호분리

  • 양한범;임재관;이일희;유재형
    • Proceedings of the Korean Nuclear Society Conference
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    • 1998.05b
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    • pp.533-538
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    • 1998
  • 추출제 Cyanex 301에 대한 NaN $O_3$ 매질에서 Am과 Eu의 추출 및 상호분리 거동에 대해 고찰하였다. Cyanex 301에 대한 Am과 Eu의 추출거동은 매우 유사하여 상호 분리할 수 없었으나 Cyanex 301을 8M NaOH로 비누화 처리하여 NaN $O_3$ 용액 매질에서 Eu에 대한 미량의 Am의 분배계수를 측정한 결과 Am에 대한 선택적 추출성이 높게 나타났다. Cyanex 301의 비누화을, 수용상의 pH 및 Eu 농도가 높아질수록 Am과 Eu의 상호 분리계수인 S $F_{AM}$Eu/는 930까지 증가되었으나 Cyanex 301에 옥탄올을 첨가할 경우에는 S $F_{Am}$ Eu/는 32.3까지 감소하였으며, Am과 Eu의 추출에 미치는 NaN $O_3$ 농도 영향은 없는 것으로 나타났다. Cyanex 301에 추출된 Am과 Eu은 pH가 4인 lM NaN $O_3$ 용액으로는 97.7% 그리고 0.05M DTPA/1.5M Lactic acid에 의해서 99% 이상 역추출되었다.

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In vitro Anti-hypertensive, Antioxidant and Anticoagulant Activities of Extracts from Haliotis discus hannai (전복(Haliotis discus hannai) 추출물의 혈압강하, 항산화능 및 항혈전능에 대한 in vitro 효과)

  • Kim, Hag-Lyeol;Kang, Seong-Gook;Kim, In-Chul;Kim, Seon-Jae;Kim, Du-Woon;Ma, Seung-Jin;Gao, Tiancheng;Li, Hua;Kim, Min-Jung;Lee, Tae-Hoon;Ham, Kyung-Sik
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.35 no.7
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    • pp.835-840
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    • 2006
  • The purpose of this study was to examine the in vitro effects of body and visceral portion of Haliotis discus hannai on angiotensin converting enzyme (ACE) activity and antioxidant and anticoagulant capacity. Extracts from both abalone body and visceral portion using 80% ethanol showed a high ACE-inhibitory effect. While ACE-inhibitory effect of extracts of the body part was dose-dependent, visceral extracts did not show any difference by the level of concentration. ACE-inhibitory effect of the visceral portion was much higher than that of the body. Antioxidant capacity was increased with increasing concentration of 80% ethanol body extracts although the capacity was low. The 80% ethanol visceral extracts showed a similar level of antioxidant capacity to the body extract in low concentration. Water extracts showed a dose-dependent increase in the activity. There was no significant difference in the antioxidant activity between the body and the visceral part. Anticoagulant capacity of 80% ethanol extracts, which was measured using prothrombin time(PT), was higher in the body part than the visceral part. Water extracts of Haliotis discus showed no any significant effect on anticoagulant capacity. The in vitro effects were also examined after Haliotis discus was refrigerated for 48 hours. Higher ACE-inhibitory effect was observed for the visceral portion than the body, in particular, before the sample was refrigerated. Antioxidant effect of Haliotis discus increased with increasing level of the sample before it was refrigerated. However, there was a significant difference between the body and the visceral part, which showed significantly higher capacity. There was no significant difference between the body and visceral part in PT regardless of refrigeration. While activated partial thromboplastin time (APTT) showed no significant difference between body and visceral part, there was a significant difference in the capacity between before and after the refrigeration, which showed much lower coagulant capacity.

Emulsification Stability of Oleoresing Red Pepper Changes in Antioxidant Activity during Thermal Cooking (고추 Oleoresin의 유화안정성 및 가열 조리중의 항산화활성 변화)

  • 최옥수
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.25 no.1
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    • pp.104-109
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    • 1996
  • 고추의 효능을 높이기 위한 방법의 하나로 향신성 분을 보유하면서 자기저자이 가능한 oleoresin을 추출하여 유화제로 수용성을 부여시킨 후, 이때의 유화안 정성과 고온에서 조리를 하였을 때 고추 oleoresin의 항산화 활성을 검토하였다. 100mesh로 분쇄한 고추에 증류수에 가하여 감압증류시켜 정유성분을 추출하고, 잔사에 에틸 알코올을 가하여 $25^{\circ}C에서$ 3시간 진탕 추출한 후 여과, 농축시켜 정유성분과 합하였다. 여기에 물과 유화제 (PGDR)를 첨가, 유화시켜 고추 oleoresin으로 하였다. $60^{\circ}C에서$ 24시간 방치했을때의 enulsion 생성율은 PGDR을 4% 첨가한 경우가 94.5%로 가장 높았고, 또한 100분간 원심분리(200$\times$g) 시킨 후에도 emulsion 잔존율은 80%정도로서 유화안전성이 가장 좋았다. 고추 oleoresin의 항산화 활성은 가열조리 온도 100$\times$의 경우 BHA의 효과 만큼은 미치지 못하나 상당한 항상화성을 나타내었고, 온도 $150^{\circ}C의$ 경우는 BHA 첨가와 마찬가지로 항산화성 효과를 인정하기 어려웠다.

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Extraction and Chemical Composition of Soluble Polysaccharide from Green Laver, Enteromorpha prolifera (가시파래 수용성 다당의 추출 및 화학적 조성)

  • Choi Yong Seok;Koo Jae Geun;Ha Jin Hwan;Yoon Jang Tak
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.35 no.5
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    • pp.519-523
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    • 2002
  • Soluble polysaccharide (SP) from green layer, Enteromorpba prolifera was extracted 3 times with distilled water at 100$^{\circ}C$ for 2 hrs and fractionated with cetylpyridinium chloride (CPC) and ion exchange chromatography (DEAE Separose CL-6B). The SP amounted to $23.7\%$ of the dry seaweed weight and contained $68.8\%$ carbohydrate. It was mainly constituted of rhamnose, glucose, xylose, sulfate and uronic acid and was fractionated with CPC into three (CPC-S, CPC-PS, CPC-PP) tractions. The major acid fraction CPC-PS accounted for $10.2\%$ of the dry algal weight. CPC-PS was further fractionated on DEAE Sepharose CL-6B into Fr-1 ($8.0\%$), Fr-2 ($35.8\%$), Fr-3 ($23.7\%$) fractions. The Fr-3 fraction contained $2.2\%$ protein, $21.4\%$ sulfate, $15.3\%$ uronic acid, and $72.4\%$ polysacchnrides composed of rhamnose, xylose and glucose. The Fr-2 fraction, which was richer in uronic acid ($17.5\%$) and poorer in sulfate ($19.0\%$) and total sugar ($68.8\%$) than the Fr-3, had a sugar composition close to that of Fr-3. The average molecular weights of Fr-2 and Fr-3 were 510,000 and 830,000 daltons, respectively. Fr-3 turned out to be homogeneous by cellulose acetate electrophoresis.

Suppressive Effect of Organic Farming Materials on the Development of Tomato Gray Mold (토마토 잿빛곰팡이병에 대한 유기농업자재의 억제효과)

  • Hong, Sung-Jun;Kim, Yong-Ki;Shim, Chang-Ki;Kim, Min-Jeong;Park, Jong-Ho;Han, Eun-Jung;Jee, Hyeong-Jin;Kim, Suk-Chul
    • Korean Journal of Organic Agriculture
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    • v.23 no.3
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    • pp.567-582
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    • 2015
  • Botrytis cinerea infects stems, leaves and fruits of greenhouse tomato and can cause serious economic losses. This study was conducted to develop organic farming control method against tomato gray mold. Twenty two organic farming materials including mineral and plant extracts were screened for the suppressive activity against Botrytis cinerea, in vitro and in vivo. Among the organic farming materials, sulfur, copper, Chinese twinleaf extract and rhubarb extract decreased by 51.7-90% of the spore germination of Botrytis cinerea. Also, gray mold incidence was reduced more than 90% on tomato stems by treating sulfur, seaweed extracts, rhubarb root extracts and Chinese twinleaf extract. After the selected four organic farming materials were applied on tomato cultivated in greenhouse, their control effects against the tomato gray mold were tested. When the water soluble sulfur was foliar-sprayed on the tomato leaves infected by artificial inoculation with spore suspension of Botrytis cinerea, it showed 87.9% of control value. Also, control activity of the water soluble sulfur was paralleled with chemical fungicide, diethofencarb+carbendazim. The above mentioned results indicate the sulfur formulation can be used as chemical fungicide alternatives for controlling tomato gray mold in the greenhouse.

Optimum pH Condition of Defatted Rice Protein Extraction by Alkaline Method (알칼리 추출법에 의한 탈지 미강 단백질 추출의 최적 pH 조건)

  • Kim, Won;Jung, So-Young;Hong, Kwang-Won
    • Food Engineering Progress
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    • v.15 no.2
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    • pp.143-147
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    • 2011
  • For efficient extraction of protein from defatted rice bran, the 5 ranges of extraction pH (8, 9, 10, 11 and 12) and the 3 ranges of isoelectric precipitation pH (2, 4 and 6) were used. The protein content, browning reaction, the electrophoresis pattern and the recovery yield of soluble protein at each pH range were compared each other. The recovery yield of soluble protein increased in proportion to extraction pH, but at the same time, browning reaction became more conspicuous. The most amount of protein was recovered at the precipitation pH of 4. The SDS-PAGE patterns of the extracted proteins showed no significant correlations between pH and the protein content, but the highly alkaline condition was more advantageous to extract protein less than 35 kDa. In each pH range, the recovery yield of soluble protein averagely reached 32.5% on the basis of extraction. In result, it was found that combination of extraction pH 10 and precipitation pH 4, which resulted in 37.65% of recovery yield and low level of browning reaction, was the optimum condition for the extraction of protein from defatted rice bran.

Effect of Buckwheat Polysaccharides on Digestive Enzyme Activity In Vitro (In vitro에서 메밀의 다당류가 소화효소 활성에 미치는 영향)

  • Lee, Jung-Sun;Ra, Kyung-Soo;Son, Heung-Soo
    • Korean Journal of Food Science and Technology
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    • v.28 no.1
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    • pp.34-39
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    • 1996
  • We examined the effects of crude hemicellulose, alcohol-insoluble hemicellulose, high molecular weight soluble polysaccharide (HMS-P : MW>10 kDa) and low molecular weight souble polysaccharide (LMS-P : MW<10 kDa) fraction isolated from buckwheat (raw, roast and steam) on digestive enzyme activity in vitro. The enzyme activities were measured after the polysaccharides-enzyme mixtures were incubated at $37^{\circ}C$ for 5 min. Crude hemicellulose, alcohol-insoluble hemicellulose and residue lowered ${\alpha}-amylase$ activity, whereas HMS-P and LMS-P had no inhibitory effect. All polysaccharides except LMS-P lowered lipase activity. Crude hemicellulose, alcohol-insoluble hemicellulose, residue and HMS-P showed a marked decrease of trypsin and chymotrypsin activity but LMS-P showed a slight decrease of them.

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