• Korean journal of applied entomology
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• v.15 no.4 s.29
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• pp.169-178
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• 1976

The concentration of amino acids, total nitrogen, trehalose, lipids and the activities of respiratory, acid$\cdot$alkaline phosphatase, glutamic oxalozcetic transaminase and glutamic pyruvic transaminase during larval stage in Pine leaf gall midge, Thecodiplosis janensis Uchi. et Inouye were measured using Paper chromatographic method, micro-Kjeldahl method, Thin layer chromatographic method, Warburg's manometric method, Bessey-Lowry method and Reitman-Frankel method, respectively. Healthy specimens )yore chosen as samples of each larval stages; alrva in gall and larva in soil. Amino acids present in the alcoholic extracts were alanine, glutamic acid, glycine, histidine, methionine, proline, threonine, tryptophan and valine. The total nitrogen concentration reached to 31.348mg/g during the larva in gall and the larval stage in soil of the value was decreased to 29.027mg/g. The hemolymph sugar, trehalose value for larva in soil was about two times of the value for larva in gall. Total lipid, phospholipid,monoacylglycerol, triacylglycerol, sterol, free fatty acid and ester cholesterol were identified at larval stages in gall and soil. Triacylglycerol concentration reached high level in contrast with other lipid contents during larvae in gall and larva in soil. Free fatty acid, sterol except decreased lipids during larval stage in soil. Endogenous respiration, succinate of respiratory activities decreased at larval stage in soil compare with larva in gall. The activities of acid phosphatase decreased larval stage in soil but the activities of alkaline phosphatase increased remarkably. The activities of glutamic oxaloacetic transaminase and glutamic pyruvic transaminase reached high level of the larva in gall.

• Journal of Korean Society of Forest Science
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• v.50 no.1
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• pp.56-71
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• 1980

To study the changes of vegetational composition of each plant successional stage of Pinus densiflora forest caused by pine gall midge damage, Thecodiplosis japonensis, located in Kyonggi-do area, the middle part of Korea, four districts, Anseong, Yongin, Gwangju, and Gapyeong, were selected according to the length of insect damage duration. The forest in the Anseong district was the noninfested one selected as the check. And forests in Yongin, Gwangju, and Gapyeong were the recent]y damaged, severely damaged, and damage prolonged ones in sequence. It was considered that this order could be in concord with the gradual transition of plant successional stage. In view of temperature, precipitation and soil factors, these four districts were sited within the same environment. Four survey plots were selected from each district and the distance between them was 3~4 km. Then again four subplots were set within a plot. Through needle accumulation after insect infestation, light intensity, soil moisture content, and the amount of soil organic matter were increased and these conditions could create a favorable environment enabling the new species invasion and their growth. These were naturally closely related with the increase in quantity of Pinus densiflora defoliation. After considerable time lapse, the quantities of above mentioned factors decreased with the development of the new substitute tree species. It was found that Quercus mongolica was an excellently substitute tree species come first in this studied area. Species diversity increased to a degree at the early stage of the duration of pine gall midge damage and decreased thereafter. As the same manner, similarity index value between plots in the same district decreased to a degree and increased thereafter. This was linked admittedly with the increase and decrease of tree species and evenness. Synthesizing above mentioned results, the process of plant succession studied quantitatively by changes of relative density and relative importance value, it can be concluded that Pinus densiflora forest gives way to Genus Quercus forest in which Genus Lespedeza and Genus Rhododendron species become as subdominance species after removal of the Pinus densiflora trees damaged by pine gall midge.

• Journal of Korean Society of Forest Science
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• v.79 no.1
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• pp.21-25
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• 1990

To investigate and analyze the change of the vegetation community structure between the severely damaged time (in 1980) and the restored time (in 1987) by the pine gall midge. Thecodoplosis japonensis, four plots with five subplots were sampled at Gwangju-gun of Kyongki-do. The total number of woody species per $500m^2$ changed 39 in 1980 to 41 in 1987. According to the damage restoring, the importance value of the genus Quercus as substituting in the severely damaged forest showed a slight decrease but the importance value of Carpinus laxiflora, Sorbus alnifolia and Styrax obassia increased. As to changes of the vegetation community structure for seven years, the diversity of vegetation structure has increased. The similarity indices between 1980 and 1987 were 52.9% and 51.9% in the understory and shrub stratum, respectively.

• Journal of Korean Society of Forest Science
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• v.77 no.3
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• pp.315-321
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• 1988

To investigate and analyze the change in the forest community structure between non-attacked time(in 1980) and severly damaged time(in 1987) by pine gall midge, Thecodoplosis japonensis, four plots with five subplots were sampled at Cheongwon-gun of Chungcheungbuk-do. The total number of woody species per 0.2ha changed 32 in 1980 to 53 in 1987. According to the damage increasing, the importance value of the genus Quercus as a substituting species has sprung up. Especially the importance value of Quercus serrata and Q. aliena singnificantly increased in the heavily insect infested forest. As to changes of forest community structure during 7 years, diversity of investigation structure has increased. The similarity indices between 1980 and 1987 were 52.9% and 49.7% in the understory and shrub stratum, respectively.

• Applied Biological Chemistry
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• v.36 no.6
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• pp.463-468
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• 1993

In order to elucidate the translocation of the systemic insecticide, phosphamidon (2-chloro-2-diethylcarbamoyl-1-methylvinyl dimethyl phosphate), treated to pine trees against pine leaf gall midges (Thecodiplosis japonensis Uchida et Inouye), $[vinyl,\;carbonyl-^{14}C]$ phosphamidon was implanted into the trunks of 10-year-old Korean red pine (Pinus densiflora Sieb. et Zucc.) and Japanese black pine (Pinus thunbergii Parl.), respectively. The upward movement rates of the chemical within Korean red pine in July and Japanese black pine in December were ca. 10 cm/hr and 2 cm/hr, respectively. The original radioactivity level persisted up to 75 days after treatment throughout the whole tree in Korean red pine in July, whereas it did up to 150 days in Japanese black pine in December. The chemical was translocated up to the top at the insecticidal level within 3 days after treatment in July, whereas in December it was within 15 days. The translocation of the chemical was much dependent upon pine tree species and the treated season. Methanol was suitable for the extraction of phosphamidon and its metabolites from pine needles. Autoradiography of the methanol extracts of pine needles collected from the treated pine trees proved that phosphamidon broke down very quickly within pine trees (ca. 80% metabolized within 7 days).

• Microbiology and Biotechnology Letters
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• v.27 no.5
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• pp.415-418
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• 1999

Thecodiplosis japonensis is an important insect pest in pine trees and hard to be controlled by chemical pesticides due to its characteristic life cycle. Experiments were conducted to determine the pathogenicity and growth temperature of several entomopathogenic fungi as potential microbial insectcides for T. japonensis. Beauveria bassiana SFB-SC and Paecilomyces fumosoroseus SFP-198 were highly pathogenic against T. japonensis. B. bassiana SFB-SC had also pathogenicity against a beneficial insect Bombyx mori. Growth rate and pathogenicity of P. funmosoroseus SFP-198 under 15$^{\circ}C$ was higher than those of B. bassians SFB-SC. These results indicated that P. fumosoroseus SFP-198 is a potential microbial control candidate for control of T. japonensis.

• Korean journal of applied entomology
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• v.19 no.1 s.42
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• pp.5-9
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• 1980

Some insecticides were evaluated on the effect of single ULV foliar spray in the control of the pine gall midges (Thecediplosis japonensis Uchida et Inouye) when the formulated (undiluted) and/or diluted insecticides were applied by ULV Sprayer (Battery-type of 12 voltage, devised by Union Carbide) With the formulated insecticide spray (30ml per plot; ten pine trees of 1.5 to 2m in height), the order of control effectiveness was Salithion (Ec 25), $Sumithion^{(R)}$ (ULV 80), Dimethoate (Ec 50), $Sevin\;oil^{(R)}$ (ULV 50), $Zolone^{(R)}$ (Ec 25) and $Folimat^{(R)}$ (Ec 50). However, except Zolone, other insecticides tested caused relatively severe phytotoxicity on the pine needles in all treatments. The dilluted insecticides (200ml Per Plot) of Salithion and Dimethoate with 10,20 and 40 times of water solution showed better control effect than with the formulated insecticides, and no phytotoxicity was observed. Salithion was more effective than Dimethoate. In conclusion, the desirable results in the pine gall midge control in this experiment were obtained by single ULV foliar spray of Salithion with 10 to 20 times of water solution, and the feasible timing of insecticide application would be from late in May to early in June.

• Journal of Korean Society of Forest Science
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• v.87 no.4
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• pp.571-576
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• 1998

Experiments were conducted to evaluate the efficacy of the application of low concentration insecticides by sprinkler system for control of the pine needle gall midge, Thecodiplasis japonensis, by killing the adults emerging and copulating near the ground. For ground spray, 2 insecticides of fenitrothion and deltamethrin, dilutions of $2,000{\times}$ and $3,000{\times}$ deltamethrin, and 3 spray intervals of 1-3 day were tested at the peak time of adult emergence during the day time. The results are as follows ; 1. Ground spray of fenitrothion 50% EC and deltamethrin 1% EC were found to be highly effective for control of the pine needle gall midge. Average gall formation by fenitrothion, deltamethrin and untreated ones were 3.40%, 5.23% and 45.69%, respectively ; control value of both insecticides exceeded 88%. 2. As a ground spray of deltamethrin 1% EC, dilution $2,000{\times}$ was significantly different from $3,000{\times}$ in gall formation rates. Average gall formation of $2,000{\times}$ plots and $3,000{\times}$ treated ones were 5.23% and 18.00% ; control values were 89.44% and 63.66%, respectively. 3. Diurnal treatment of ground spray was found to be highly effective for control of pine needle gall midge. 4. In particular, control by ground application of dilutions $2,000{\times}$ and $3,000{\times}$ of deltamethrin is suggested because it does not have an adverse effect on arthropods of the ground surface such as ants and spiders.

• Journal of Korean Society of Forest Science
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• v.79 no.2
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• pp.115-126
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• 1990

This study was performed to find differences in phototaxis response of the larva, adult emergence, sex ratio and gall formation rate of Thecodiplosis japonensis Uchida et Inouye between two areas, north and south. Larvae from these two areas, Hweng-sung in north and Moo-an in south, both considered to be the sities of severe outbreak of the insect recently, were collected from the floor and transported to the entomology larboratory, Won-Kwang University in Iri, then overwintered. These larvae were used for various experimental purposes on various dates. The following results were obtained. 1. The sizes of larvae reached 2.45 in length, 0.70 in width for north, and 2.50 in length, 0.72 in width for sourth. It seems that the differents were not significant. 2. It is known that Leaving gall of larvae to the ground for the hibernation usually occurs on rainy days. For the larvae dispersion, the phototaxis response of the larvae was hypothesized. To check this, the author manipulated some different illumination intensities adjusting the distance between the glass tube in which 100 larvae were contained and electric bulb. The glass tubes were blackened all but except a small portion. The phototaxis responses between two areas seemed to be different particularly at low light intensities, 45, 145 and 1000 lux, The larvae from north assembled to the clear portion of the glass tube were more than 30 out of 100, however less than 30 in south sample regardless of the time passage. In either cases, the saturation points came after about 8 to 10 hours. If temperature units were used, the phototaxis curve= after 1 hour illumination between two areas provided significant differences. 3. The adult emergence of south area was lagged 10 days later than that of north area. The accumulated effective day-degrees of temperature for adult emergence were $934^{\circ}C$ for north area and $1180^{\circ}C$ for south area. The emerging duration of north area appeared to be in late May through the early of July and the peak emergence occurred in middle June that was approximately 15days earlier than that of south area. The sex ratio of female to male, regardless of area, was approximately 3 : 1. 4. The rates of gall formation of the red pine(Pinus densiflora Siebold et Zuccarini), 6-year-old seedlings, were as low as 9.94% for north area and 8.87% for south area. Through the close observation, the author presumed that the population reduction was greatly affected during the prepupa stage by relative moisture content and predators, such as spiders and ants presented on the ground.

• Korean journal of applied entomology
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• v.18 no.4 s.41
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• pp.153-160
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• 1979

The present experiments were carried out to reduce the population density of adult pine gall midge, Thecodiplosis japonensi,s UCHIDA et INOUYE, by means of spray the sticky agent C-4, on the ground, herbacious plantation and foliar leaf of pine trees at Sanbonli,. Anyang, Kyungido. And also the 3 periods of treatment, such as before 2 weeks( I ), before 1 week (II) from the peak emergence period and peak emergence period (III) were applied. The experimental results obtained were as follows. (1) The formula of selected sticky agent C-4 was $70\%$ of castor oil, $25\%$ of damar resin and $5\%$ of carnauba wax, and it showed the best both on stickiness and duration, and lower phytotoxicity to the host plant. (2) The reduction of population densities of the adult PGM were $68\%$ and 78f: in the G-I and G-II treatment plot respectively, which compared to control, on the ground spray. (3) And the reduction of population densities were $63\%$ and about $90\%$ in the P-I and P-II plot respectively when the agent was sprayed on the herbacious plantation. (4) The rates of gall formation were $32.8\%,\;40.8\%\;and\;59.4\%$ in the spray plots of F-I, F-II, and F-III respectively, and there was no significant difference among the upper, middle and lower parts of the treated host plant in the rates of gall formation. (5) The effective stage of sticky agent application were considered as before one week from the peak emergence period in tile all types of treatment.