• Korean Journal of Ichthyology
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• v.33 no.2
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• pp.45-56
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• 2021

A male, presumed to be an intergeneric hybrid between Tanakia lanceolata and Rhodeus pseudosericeus, was collected in the Boryeong Daecheoncheon Stream flowing into the Yellow Sea in the Republic of Korea. Morphological and molecular phylogenetic analyses were performed to discriminate the definite origin of the estimated natural hybrid. As a result of the morphological analysis, the color of the dorsal and anal fin rays edges of the natural hybrid individual, the upper and lower body colors followed the morphological characteristics of T. lanceolata, and that blue longitudinal stripe in the center of the caudal peduncle, the incomplete lateral line, and the barbels absent followed the morphological characteristics of R. pseudosericeus. In addition, as a result of the cytochrome b (cytb) gene analysis of mitochondrial DNA (mtDNA), the natural hybrid showed a nucleotide sequence similarity of 99.82 to 100% with T. lanceolata, and the maternal species was identified as T. lanceolata. As a result of the recombination activating gene 1 (rag1) gene analysis of nuclear DNA (nDNA), the natural hybrid showed double peaks pattern reflecting both the single nucleotide polymorphism sites (38 bp) between T. lanceolata and R. pseudosericeus, and the paternal species was identified as R. pseudosericeus. Therefore, a natural hybrid estimated male of Acheilognathinae analyzed in this study was found to be an intergeneric hybrid between a female T. lanceolata and a male R. pseudosericeus.

• Korean Journal of Ichthyology
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• v.3 no.1
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• pp.42-47
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• 1991

A suspected intergeneric hybrid cross between Pungtungia herzi and Pseudopungtungia nigra was collected at the Ungcheon River. Overall hybrid index did not show the hybrid connection clearly, but the most striking key character between two genus, pigmentation on the all fins except pectoral, the shape and position of the mouth part appeared to show the intermediary. So this specimen was thought to be an intergeneric natural hybrid which merits further study.

• Korean Journal of Ichthyology
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• v.22 no.4
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• pp.225-229
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• 2010

A intergeneric hybrid between Rhodeus uyekii and Acheilognathus signifer was collected in the Jojongcheon Bukhan River. The hybrid showed intermediate characteristics of two parental types in some key characters, such as color pattern of body, pigmentation of dorsal, anal, and caudal fins, and body spot and stripe. In addition, the hybrid had a fairly large number of lateral line scales, while it lacked a barbel, which is known as a key intergeneric character. The probable occurrence of a natural hybrid is supported by a viable $F_1$ hybrid produced with the parental species.

• Korean Journal of Ichthyology
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• v.26 no.3
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• pp.153-158
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• 2014

A natural intergeneric hybrid between Rhodeus pseudosericeus and Acheilognathus signifer was collected in their sympatric area, the Jucheongang (stream), the Namhangang (river), Korea. Morphological characters as well as mitochondrial cytochrome b (mt-cyb) gene were investigated to confirm the natural hybrid origin. In the comparison of morphological characters, the natural hybrid appeared to have intermediate characters between two parental species in several key characters such as the band of dorsal fins, the color pattern of body, and the body spot and stripe. Also, the collected specimen had an incomplete lateral line with a large number of pored scales. In analysis of mt-cyb gene, it revealed that the maternal species of the natural hybrid appeared to be R. pseudosericeus due to their 100% sequence identity. Thus, such results gave convincing evidence for the occurrence of a natural intergeneric hybrid between Rhodeus pseudosericeus and Acheilognathus signifer.

• Korean Journal of Plant Tissue Culture
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• v.22 no.5
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• pp.261-266
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• 1995

This study was carried out to develop new varieties which are dwarf and tolerant to winter cold in the Aurantioideae by intergeneric crossing. to do that, the reciprocal crosses of Hwanggeumyooza and trifoliate orange, yooza and trifoliate orange were done and in vitro immature ovule culture of their hybrid was carried out .The callus formation from immature ovule was good in order of Hwanggeumyooza, Hwanggeumyooza $\times$ tifoliate orange, yooza, and trifoliate orange and best at 1 to 3 mg/L NAA+0.5mg/L zeatin on MT medium. In vitro germination percentage of 20week old hybrid of Hwanggeumyooza $\times$ tifoliate orange and trifoliate orange $\times$ Hwanggeumyooza were 41.3% and 37.7, respectively. The phenotype of hybrid (95%) of Hwanggeumyooza $\times$ trifoliate orange and that (100%) of trifoliate orange $\times$ Hwanggeumyooza were similar to that of trifoliate orange. After Hwanggeumyooza was pollinated by pollens of trifoliate orange, the pollen tubes grew on stigma after 3h of pollination and entered into micropyle after about 24~28 h. One gamete in pollen was fused with polar nuclei after 2 days and other one fused with egg nucleus at 3days after pollination. The fruit set percentage by intergeneric crossing was 14.0% in Hwanggeumyooza $\times$ trtfoliate orange and 17.5% in trifoliate orange $\times$ Hwanggeumyooza. The fruit set percentages of Hwanggeumyooza. and trifoliate orange were 34.2% and 39.5% by artificial self-fertilization, 34.2% and 39.5% by artificial cross fertilization, 3.1% and 1.4% by parthenocarpy and 13.0% and 3.0% by natural fertilization, respectively. The somatic and gametic chromosome numbers of Hwanggeumyooza, yooza, and trifoliate orange were 2n=18 and n=9.

• Monthly Duck's Village
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• s.56
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• pp.54-56
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• 2008

오리 종란을 수집하기 위해서 이용하는 가장 보편적인 사육 방법은 암.수를 일정한 비율로 합사하는 것으로 자연교미를 이용하는 것이다. 그러나 우수한 종오리나 특정 합성종오리(종간 혹은 속간 잡종)를 생산하기 위해서는 인위적인 인공수정기술을 이용하여 계획교배를 하여야 하며, 이를 위해서 선발된 오리들은 케이지 사육을 하여야 한다. 오리 인공수정기술이 닭의 인공수정기술과는 숫오리의 정액 채취기술에서만 차이가 있고 다른 부분에서는 매우 유사하므로 정액채취부분을 중심으로 설명을 하고자 한다.

• FLOWER RESEARCH JOURNAL
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• v.17 no.1
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• pp.40-43
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• 2009

'Glory Pink' an interspecific lily cultivar was released in 2007 at National Horticultural Research Institute (NHRI), Rural Development Administration (RDA), Suwon, Korea. The cross and ovule culture was made between FA interspecific hybrid 'FA97-30' (L. formolongi 'Raizan' ${\times}$ Asiatic lily 'A61'), a red colored cultivar, and Asiatic lily 'Sanzio', light red colored cultivar in 2001. Multiplication and bulbing, and characteristic tests were conducted from 2005 to 2006. Flowering time is around the first of July and the plant height is 131.7 cm. Flowers are upward-facing and red-pink (RHS 63C), with size of 11.3 cm. Petal length and width is 9.1 cm and 4.1 cm, respectively. Leaves are 13.8 cm long, 1.4 cm wide. The throat is dark green, and the stigma is light yellow and pollen is light brown. The weight and size of bulb is 51.1 g and 13.2 cm, respectively. For long-term storage, bulbs can be stored under -1 to -2 for year-round forcing.

• Journal of Plant Biology
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• v.6 no.1
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• pp.1-7
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• 1963

Two individuals of intergeneric hybrids in the crossing of Brassica pekinensis ♀$\times$Raphanus sativus ♂ were obtained, and among the three kinds of plants, Brassica, Raphanus, and F1, comparison on the mega- and microsporogenesis, megagammetophyte formation, and morphological changes in the developing sporangia, etc., were made. Differences between Brassica and Raphanus were observed in the shape of mega- and microsporangia, their changes in development, and the formation of megagametophyte. Sporangia of the F1 plants, until pre-meiotic stage, show intermediate, maternal, paternal, or vigorous inclination, and the difference of these characteristics when compared with those of the parent is slight. Meiotic irregularity resulted from the intergeneric hybridity of F1 plant gives rise to the abnormal mega- and microspore and accompanied abortive female and male gametophytes, bringing about the remarkable differences from its parent in the morphological changes of the developing mega- and microsporangia.

• Journal of Plant Biology
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• v.30 no.1
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• pp.1-9
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• 1987

Leaf mesophyll protoplasts of Nicotiana tabacum (nitrate reductase deficient mutant) were fused with cell suspension protoplasts of albino Petunia inflata in an electric field. Hybrid cell colonies were selected for nitrate reductase proficiency and chlorophyll synthesis. Five hybrid plant lines, regenerated from the selected calli lines, were analysed by electrophoresis, number of chromosomes and morphological characters. Somtic hybrid plants showed both parent patterns in the isozymesof isoleucine aminopeptidase and esterase. The hybrids had the expected chromosome number of 62 and exhibited an intermediate floral morphology when compared with the parents, but plant height and leaf arrangement were similar to N. tabacum.

• Korean Journal of Plant Tissue Culture
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• v.22 no.6
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• pp.311-316
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• 1995

In an attempt to obtain intersubgeneric somatic hybrids of Pelargonium aridum and P.zonale, protoplast isolated from the two species were fused by using polyethylene glycol(PEG) and electorfusion methods. Protoplast were isolated from cotyledon and leaf tissues using MS medium containing 550 mM sucrose, 0.7% cellulase (Onozuka R-10) and 0.4% Macerozyme. The optimum number of protoplasts per mL of culture medium was 6 x 10$^4$. Protoplast fused by the electrofusion method were more active than by PEG method. Heterokaryotically fused protoplasts formed calli when cultured in MS medium containing 550 mM glucose, 1 to 2 mg/L NAA and 0.5 to 1 mg/L BA.