Korean Journal of Plant Tissue Culture (식물조직배양학회지)
- Volume 22 Issue 6
- /
- Pages.311-316
- /
- 1995
- /
- 1015-5880(pISSN)
Intersubgeneric Protoplast Fusion of Pelargonium aridum (Ligularia) and P.zonale(Ciconium)
Pelargonium aridum과 P. zonale 아속간의 원형질체융합
Abstract
In an attempt to obtain intersubgeneric somatic hybrids of Pelargonium aridum and P.zonale, protoplast isolated from the two species were fused by using polyethylene glycol(PEG) and electorfusion methods. Protoplast were isolated from cotyledon and leaf tissues using MS medium containing 550 mM sucrose, 0.7% cellulase (Onozuka R-10) and 0.4% Macerozyme. The optimum number of protoplasts per mL of culture medium was 6 x 10
Pelargonium aridum과 P. zonale 아속간의 체세포 잡종을 얻기 위하여 이들로부터 원형질체를 유리하여 polyethylene glycol (PEG)와 electrofusion 방법으로 융합시켰다. 원형질체는 자엽과 본엽을 550 mM sucrose, 0.7% cellulase (OnozukaR-10), 0.4% Macerozyme이 함유된 MS 배지로 유리하였다. 배양밀도는 배지 mL당 원형질체수는 6 x