• Title/Summary/Keyword: 셀 형성

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Direct Conversion of L-Selenomethionine into Methylselenol by Human Cystathionine ${\gamma}$-Lyase (인간 Cystathionine ${\gamma}$-Lyase에 의한 Selenomethionine의 Methylselenol로의 직접분해)

  • Cho, Hyun-Nam;Jhee, Kwang-Hwan
    • Microbiology and Biotechnology Letters
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    • v.42 no.1
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    • pp.11-17
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    • 2014
  • Selenium is an essential trace element for mammals, but it is very toxic. Therefore, the control of selenium concentrations should be precisely and effectively monitored. Selenium is naturally obtained through foods and seleno-L-methionine (LSeMet) is a major form of selenium. It has been reported that L-SeMet is only converted into Se-adenosyl-L-SeMet. However, a recent study suggested that L-SeMet was directly metabolized into methylselenol ($CH_3SeH$) in mouse liver extract by the reaction of cystathionine ${\gamma}$-lyase (CGL). The canonical reaction of CGL was known to catalyze the cleavage of L-cystathionine to L-cysteine, ${\alpha}$-ketobutyrate and $NH_3$. In the present study, we found that L-SeMet could be directly converted to $CH_3SeH$ using purified homogenous human CGL instead of mouse liver cytosol. Authentic $CH_3SeH$ was prepared by reduction of dimethyldiselenide with sodium tetrahydroborate. The gaseous product of the enzymatic reaction with L-SeMet was analyzed by GC/MS spectrometry. The GC/MS data was identical to that of authentic dinitrophenyl selenoether. We also analyzed the kinetic parameters for the formation of $CH_3SeH$ from L-SeMet by human and mouse CGL. These results suggest that human CGL is a critical enzyme which is responsible for L-SeMet metabolism.

Study on the Geochemical Weathering Process of Sandstones and Mudstones in Pohang Basin at CO2 Storage Condition (지중저장 조건에서 초임계CO2에 의한 포항분지 사암과 이암의 지화학적 풍화반응 연구)

  • Park, Jinyoung;Lee, Minhee;Wang, Sookyun
    • Economic and Environmental Geology
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    • v.46 no.3
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    • pp.221-234
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    • 2013
  • Laboratory experiments for the reaction with supercritical $CO_2$ under the $CO_2$ sequestration condition were performed to investigate the mineralogical and geochemical weathering process of the sandstones and mudstones in the Pohang basin. To simulate the supercritical $CO_2$-rock-groundwater reaction, rock samples used in the experiment were pulverized and the high pressurized cell (200 ml of capacity) was filled with 100 ml of groundwater and 30 g of powdered rock samples. The void space of the high pressurized cell was saturated with the supercritical $CO_2$ and maintained at 100 bar and $50^{\circ}C$ for 60 days. The changes of mineralogical and geochemical properties of rocks were measured by using XRD (X-Ray Diffractometer) and BET (Brunauer-Emmett-Teller). Concentrations of dissolved cations in groundwater were also measured for 60 days of the supercritical $CO_2$-rock-groundwater reaction. Results of XRD analyses indicated that the proportion of plagioclase and K-feldspar in the sandstone decreased and the proportion of illite, pyrite and smectite increased during the reaction. In the case of mudstone, the proportion of illite and kaolinite and cabonate-fluorapatite increased during the reaction. Concentration of $Ca^{2+}$ and $Na^+$ dissolved in groundwater increased during the reaction, suggesting that calcite and feldspars of the sandstone and mudstone would be significantly dissolved when it contacts with supercritical $CO_2$ and groundwater at $CO_2$ sequestration sites in Pohang basin. The average specific surface area of sandstone and mudstone using BET analysis increased from $27.3m^2/g$ and $19.6m^2/g$ to $28.6m^2/g$ and $26.6m^2/g$, respectively, and the average size of micro scale void spaces for the sandstone and mudstone decreased over 60 days reaction, resulting in the increase of micro pore spaces of rocks by the dissolution. Results suggested that the injection of supercritical $CO_2$ in Pohang basin would affect the physical property change of rocks and also $CO_2$ storage capacity in Pohang basin.

Study of system using load cell for real time weight sensing of artificial incubator (인공부화기의 실시간 중량감지를 위한 로드셀을 이용한 시스템 연구)

  • jeong, Jin-hyoung;Kim, Ae-kyung;Lee, Sang-Sik
    • The Journal of Korea Institute of Information, Electronics, and Communication Technology
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    • v.11 no.2
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    • pp.144-149
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    • 2018
  • The eggs are incubated for 18 days through the generator and incubated in the developing incubator. During the developmental period, the weight loss of the fetus is correlated with the ventricular formation, and the proper ventricular formation is also associated with the healthy embryonic hatching and the egg hatching rate. However, in the incubator period of the domestic hatchery, it is a reality to acquire the resultant side by the Iranian standard weight measurement with the experience of the hatchery and the person concerned and the development period without the apparatus for measuring the present weight. As a result, prevalence of early mortality, hunger and illness during hatching are frequent. Monitoring the reduction of weaning weight is crucial to obtaining chick quality and hatching performance with weight changes within the development machine. Water loss is different depending on the size of eggs, egg shell, and elder group. We can expect to increase the hatching rate by measuring the weight change in real time and optimizing the ventilation change accordingly. There is a need to develop a real-time measurement system that can control 10 to 13% reduction of the total weight during hatching. The system through this study is a way to check the one - time directly when moving the existing egg, and it is impossible to control the measurement of the fetal water evaporation within the development period. Unlike systems that do not affect the hatching rate, four load cells are connected in parallel on the Arduino sketch board and the AT-command command is used to connect the mobile phone and computer in real time. The communication speed of Bluetooth was set to 15200 to match the communication speed of Arduino and Hyper-terminal program. The real - time monitoring system was designed to visually check the change of the weight of the fetus in the artificial incubator. In this way, we aimed to improve the hatching rate and health condition of the hatching eggs.

A Study on the Influence of the Drawing Style of Comics in Animation (만화 그림체가 애니메이션에 미치는 영향)

  • Kim, Ji-Hong
    • Proceedings of the Korea Contents Association Conference
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    • 2006.11a
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    • pp.223-226
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    • 2006
  • It can formulate a basic concept to the study on styles of drawing on animation which is influenced by styles of drawing on comic. Especially the transferability of comic and eel animation have high possibility because they have common part as drawing, continuity of imagery and narrative, in spit of the differentiation with comic for spatial and animation for temporal. So the study on comic drawing styles are inevitable check point for investigating it's animation. To analyse of the style of animation drawing, can be employed the styles of comic drawing. For a case study, comic authors and their works is utilized for establishing basic concept of transferring comic to animation with the influence of drawing styles to animation and than it will apply to classify and analyse animation by them. The consequence of this study, it can be sorted in the three areas that are the cheerful comic, the drama, the boy-meets-girl comic. and can be proved that the close relationship of comic and animation. Among three aspects, a boy-meets-girl comic can not be detected in any selected animations. The presumption of this result that can be apply to the general idea which is a rare case to use in animation. Also to use partly cheerful comic to drama style as different drawing styles is for elucidating the effect of intending isolation with the play theory of Bertolt Brecht and empathizing dramatical situation through the disparity of emotion. This study is to investigate on the influence of the drawing style of comics in animation, and can provide the basic ideas of inter-relation of media through drawing styles study with the concept of hybrid.

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Broadband Hybrid Antenna with Directional Radiation Pattern Utilizing Epsilon Negative Zeroth-Order Resonance (ENG 영차 공진을 이용하여 대역폭이 개선되고 지향성 방사 패턴을 갖는 하이브리드 안테나)

  • Kim, In-Ho;Lee, Jeong-Hae
    • The Journal of Korean Institute of Electromagnetic Engineering and Science
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    • v.21 no.8
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    • pp.886-892
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    • 2010
  • In this paper, the hybrid antenna utilizing epsilon negative zeroth-order resonance(ENG ZOR) and $TM_{010}$-mode is presented. The antenna has a directional radiation pattern and improved bandwidth. To obtain a ENG ZOR and $TM_{010}$-mode, the hybrid antenna employs the mushroom structure and the microstrip patch, respectively. Two antennas of the mushroom and the patch are coupled by gap and fed by one coaxial feed. The frequencies of ENG ZOR and of $TM_{010}$ resonance are designed to be 4 GHz and 3.9 GHz, respectively. Because two resonant frequencies are set to be close, the dual-resonance can be formed, resulting in the broader bandwidth. Even though the radiation pattern of an ENG ZOR antenna is omnidirectional, the directional radiation of a microstrip patch antenna compensates the null of omnidirectional pattern of an ENG ZOR antenna. Thus, the hybrid antenna has a directional radiation pattern. The antennas having 4, 3, and 2 unit cells of mushroom structure are designed and analyzed. The antennas have fractional bandwidths of 4.29~4.95 %, gains of 3.16~5.57 dBi, and radiation efficiencies of 62.4~94.2 %.

A Study on Predictive Traffic Control Algorithms for ABR Services (ABR 서비스를 위한 트래픽 예측 제어 알고리즘 연구)

  • 오창윤;장봉석
    • Journal of Internet Computing and Services
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    • v.1 no.2
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    • pp.29-37
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    • 2000
  • Asynchronous transfer mode is flexible to support multimedia communication services using asynchronous time-sharing and statistical multimedia techniques to the existing data communication area, ATM ABR service controls network traffic using feedback information on the network congestion situation in order to guarantee the demanded service qualities and the available cell rates, In this paper we apply the control method using queue length prediction to the formation of feedback information for more efficient ABR traffic control. If backward node receive the longer delayed feedback information on the impending congestion, the switch can be already congested from the uncontrolled arriving traffic and the fluctuation of queue length can be inefficiently high in the continuing time intervals, The feedback control method proposed in this paper predicts the queue length in the switch using the slope of queue length prediction function and queue length changes in time-series, The predicted congestion information is backward to the node, NLMS and neural network are used as the predictive control functions, and they are compared from performance on the queue length prediction. Simulation results show the efficiency of the proposed method compared to the feedback control method without the prediction, Therefore, we conclude that the efficient congestion and stability of the queue length controls are possible using the prediction scheme that can resolve the problems caused from the longer delays of the feedback information.

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Thermal-Hydraulic Analysis and Parametric Study on the Spent Fuel Pool Storage (기사용 핵연료 저장조에 대한 열수력 해석 및 관련 인자의 영향 평가)

  • Lee, Kye-Bock;Nam, Ki-Il;Park, Jong-Ryul;Lee, Sang-Keun
    • Nuclear Engineering and Technology
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    • v.26 no.1
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    • pp.19-31
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    • 1994
  • The objective of this study is to conduct a thermal-hydraulic analysis on the spent fuel pool and to evaluate a parametric effect for the thermal-hydraulic analysis of spent fuel pool. The selected parameters are the Reynolds Number and the gap flow through the oater gap between fuel cell and fuel bundle. The simplified flow network for a path of fuel cells is used to analyze the natural circulation phenomenon. In the flow network analysis, the pressure drop for each assembly from the entrance of the fuel rack to the exit of the fuel assembly is balanced by the driving head due to the density difference between the pool fluid and the average fluid in each spent fuel assembly. The governing equations ore developed using this relation. But, since the parameters(flow rate, pressure loss coefficient, decay heat, density)are coupled each other, iteration method is used to obtain the solution. For the analysis of the YGN 3&4 spent fuel rack, 12 channels are considered and the inputs such as decay heat and pressure loss coefficient are determined conservatively. The results show the thermal-hydraulic characteristics(void fraction, density, boiling height)of the YGN 3&4 spent fuel rack. There occurs small amount of boiling in the cells. Fuel cladding temperature is lower than 343.3$^{\circ}C$. The evaluation of parametric effect indicates that flow resistances by geometric effect are very sensitive to Reynolds number in the transition region and the gap flow is negligible because of the larger flow resistance in the gap flow path than in the fuel bundle.

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Adhesion Layer 사용으로 인한 Si Thin Film Anode 전극의 신뢰성 향상

  • O, Min-Seop;Song, Yeong-Hak;U, Chang-Su;Jeong, Jun-Ho;Hyeon, Seung-Min;Lee, Hu-Jeong
    • Proceedings of the Korean Vacuum Society Conference
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    • 2013.02a
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    • pp.681-682
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    • 2013
  • 전기는 우리 주변의 에너지 형태 중에서 가장 편리하고 광범위하게 사용되고 있다. 이러한 전기는 전자제품, 전기자동차, 에너지 저장 플랜트 등 매우 많은 분야에서 저장되고 사용되고 있다. 특히 에너지 저장 용량의 확대는 휴대폰, 노트북 PC 등 휴대용 IT 기기의 성장에 결정적인 역할을 하였다. 가볍고 작으면서도 고용량의 전기 에너지 저장 장치가 없었다면, 통신이나 인터넷 그리고 오락 등 다양한 기능을 작은 휴대용 기기에 구현할 수 없었을 것이다. 그러나 시간이 흐를수록 기기의 요구 성능이 높아지고 소비자의 니즈가 더욱더 다양해지고 고도화될수록 단일 부품으로 가장 큰 부피를 차지하는 에너지 저장 장치의 용량과 디자인은 점점 중요해지고 있다. 이러한 에너지 저장 장치에서 가장 친숙한 형태는 2차 전지 계열이다. 납 축전지를 비롯하여, 니켈수소, 니켈카드뮴, electrochemical capacitor와 Li ion 계열 등이 대표적이다. 특히 Li ion 배터리는 모바일, 자동차 및 에너지 저장 그리드 등과 같은 다양한 분야에 가장 많이 적용되고있다. Li ion 배터리에 대하여 현재의 핵심적인 연구분야는 전극 재료(cathode, anode)와 electrolyte에 대한 것이다. Anode 전극 재료 중에서 가장 많이 사용되는 재료는 카본을 기반으로 하는 재료로 안정성에 대한 장점이 있지만 에너지 밀도가 낮다는 단점이 있다. 에너지 저장 용량 증가에 대한 필요성이 증가하기 때문에 현재 많이 사용되고 있는 에너지 밀도가 낮은 카본 재료를 대체하기 위해서 이론 용량이 높다고 알려진 실리콘과 같은 메탈이나 주석 산화물과 같은 천이 금속 산화물에 대하여 많은 연구가 진행되고 있다. 특히 현재까지 알려진 많은 재료 중에서 가장 큰 capacity (~4,000 mAh/g)를 가지고 있다고 알려진 실리콘이 카본의 대체 재료로 많은 연구가 진행되고 있다. 그러나, Li 과 반응을 하며 약 300~400%에 달하는 부피팽창이 발생하고, 이러한 부피 팽창 때문에 충 방전이 진행됨에 따라 current collector로부터 박리되는 현상을 보여 빠른 용량 감소를 보여주고 있다. 본 연구에서는 adhesion layer를 current collector와 실리콘 전극 재료 사이에 삽입하여 충 방전 시 부피팽창에 의한 미세구조의 변화와 electrochemical 특성에 대한 영향을 알아보았다. 실험에 사용한 anode 전극은 상용 Cu foil current collector에 RF/DC magnetron 스퍼터링을 통해 다양한 종류(Ti, Ta 등)의 adhesion layer과 200 nm 두께의 Si 박막을 증착하였다. 또한 Bio-logic Potentiostat/ Galvanostat VMP3 와 WanAtech automatic battery cycler 장비를 사용하여 0.2 C-rate로 half-cell 타입의 코인 셀로 조립한 전극에 대한 충 방전 실험을 진행하였다. Adhesion layer의 사용으로 인해 실리콘 박막과 Cu current collector 사이의 박리 현상을 줄여줄 수 있었고, 충 방전 시 Cu 원자의 실리콘 박막으로의 확산을 통한 brittle한 Cu-Si alloy 형성을 막아 줄 수 있어 큰 특성 향상을 확인할 수 있었다. 또한, 리튬과 실리콘의 반응을 통한 형태와 미세구조 변화를 SEM, TEM 등의 다양한 장비를 사용하여 확인하였고, 이를 통해 adhesion layer의 사용이 전극의 특성향상에 큰 영향을 끼쳤다는 것을 확인할 수 있었다.

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The Study of Surface Plasmonic Bands Using Block Copolymer Nanopatterns (블록공중합체 나노패턴을 이용한 표면 플라즈몬 연구)

  • Yoo, Seung Min
    • Journal of the Korea Academia-Industrial cooperation Society
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    • v.18 no.11
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    • pp.88-93
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    • 2017
  • It is important to develop a simple method oftuning localized surface plasmon resonance(LSPR) properties, due to their numerous applications. In addition, the careful examination of the shape, size and combination of metal nanoparticles is useful for understanding the relation between the LSPR properties and metal nanostructures. This article describes the dependence of theLSPR properties on the arrays of metal nanoparticles obtained from a block copolymer(BCP) micellar thin film. Firstly, two different Au nanostructures, having a dot and ring shape, were fabricated using conventional block copolymer micelle lithography. Then, Ag was plated on the Au nanostructures through the silver mirror reaction technique to obtain Au/Ag bimetallic nanostructures. During the production of these metallic nanostructures, the processing factors, such as the pre-treatment by ethanol, silver mirror reaction time and removal or not of the BCP, were varied. Once the Au nanoparticles were synthesized, Ag was properly plated on the Au, providing two distinguishable characteristic plasmonic bands at around 525nm for Au and around 420nm for Ag, as confirmed bythe UV-vis measurements. However, when a small amount of Au seed nanoparticles, which accelerate the Ag plating speed,was formed by usinga block copolymer with a relatively highmolecular weight, all of the Au surfaces were fully covered by Ag during the silver mirror reaction, showing only the characteristic peak for Ag at around 420nm. The Ag plating technique on Au nanoparticles pre-synthesized from a block copolymer is useful to study the LSPR properties carefully.

Electricity Generation and Microbial Community variation in Microbial Fuel Cell with various Electrode Combinations. (다양한 탄소전극조합에 따른 미생물 연료전지의 전기발생량 및 미생물 군집변화)

  • Kwon, Jae-Hyeong;Choi, Soo-Jung;Cha, Jae-Hwan;Kim, Hyo-Soo;Kim, Ye-Jin;Yu, Jae-Cheul;Kim, Chan-Won
    • Journal of Korean Society of Environmental Engineers
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    • v.32 no.1
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    • pp.87-96
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    • 2010
  • The electrode material is one of the factors affecting the power production of microbial fuel cell. In this study, effects of carbon electrode material, thickness and configuration on the power density, biofilm formation and microbial community diversity of microbial fuel cell were investigated. To optimize the anode-cathode electrode assembly, seven lab-scale reactors which had various carbon electrode constructions were operated in continuous mode. Under the steady state condition, the electrode combination of graphite felt (6 mm) with hole showed the highest cell voltage of 238 mV and the coulombic efficiency of 37%. As a result of SEM analysis, the bacteria growing on surface of knitted type of carbon cloth and graphite felt electrode ncreased significantly. The change of dominant species between seeding sludge and biofilm on the surface of anode electrode, microbial analysis with PCR-DGGE showed that the dominant species of seeding sludge are quite different from those of biofilm on the surface of each anode electrode. Especially Geobacter sp., a well known electrochemical bacteria, was found as the dominant species of the electrode combination with graphite felt.