• KSBB Journal
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• v.14 no.4
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• pp.470-475
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• 1999

Several enzyme were applied to Laubholz Bleached Kraft Pulp(LBKP) to evaluate the influence on beatability which was measured in Schopper Riegler value, and the results were compared with untreated pulp. Among the types of enzyme, cellulase was found to be the most effective. Addition of cellulase increased the beatability by 28% at optimum condition. Strength properties such as tensile strength and folding endurance also increased with enzymatic treatment by 12% and 46%, respectively. However, excessive dosage of cellulase had an adverse effect on strength properties in spite of the high beatability. Fibrillization by cellulase and destruction of fiber by excessive reaction was observed by Scanning Electron Microscopy(SEM).

• Journal of the Korean Home Economics Association
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• v.38 no.10
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• pp.133-142
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• 2000

The subjective hand and preference of enzyme hydrolyzed denim fabrics were evaluated using the scale developed. The factors affecting consumer's taste for polyester fabrics were analyzed by the statistical technique. The effects of alkaline hydrolysis on the properties of polyester fabrics were evaluated by subjective and objective hand measurements. The lower the age of evaluators, the more sensitive their feeling. As the age decreased, evaluators felt fabrics become flossy and soft surface property is smooth, flexible, warm, refined. They didn't catch the sense of durability, moisture related property and sense of shape recovery. The lower the age, the fabric was more preferred. Women were more sensitive and consistent than men.

• Microbiology and Biotechnology Letters
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• v.30 no.2
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• pp.172-176
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• 2002

A filamentous microorganism, strain FJ1, was isolated from completely rotten wood for the production of cellulolytic enzymes. For the production of the enzymes, cellulolsic wastes were used as carbon sources of strain FJ1 and rice straw showed higher enzyme activities than sawdust and pulp. The activities of CMCase, xylanase, $\beta$-glucosidase, and avicelase were 2.95, 5.89, 0.45, and 0.12 unit/ml by use of rice straw, respectively. To enhance production of the enzymes, the mixture substrate of rice straw and cellulosic materials were investigated as carbon sources. The highest activities of CMCase, $\beta$-glucosidase, and avicelase were found in the mixture of rice straw (0.5%, w/v) and avicel (0.5%, w/v), and the highest xylanase was obtained at the mixture ratio of 0.71%(w/v) and 0.29%(w/v). Addition of 0.1%(w/v) peptone showed enhanced production of the cellulolytic enzymes in which the activities of CMCase, xylanase, $\beta$-glucosidase, and avicelase were 19.23, 27.18, 1.28, and 0.53 unit/ml, respectively. The production of the enzymes using rice straw was efficiently induced in the presence of avicel and pulp containing cellulose. In particular, a medium composed of rice straw (0.5%, w/v) and pulp (0.5%, w/v) yielded larger cellulolytic enzymes: CMCase 24.3 unit/ml, xylanase 38.7 unit/ml, $\beta$-glucosidase 1.5 unit/ml, and avicelase 0.6 unit/ml. The filamentous microorganism, strain FJ1 utilized various cellulosic wastes as carbon sources and will be expected as a favorable candidate for biological saccharification of cellulosic wastes.

• Journal of Korean Society of Forest Science
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• v.98 no.3
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• pp.305-310
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• 2009

Yellow poplar was selected a promising biomass resources for bio-ethanol production through alkali prehydrolysis, enzymatic saccharification and fermentation using commercial cellulase mixtures (Celluclast 1.5L and Novozym 342 mixtures) and fermenting yeast. In alkali prehydrolysis, 51.1% of Yellow poplar biomass remained as residues, which chemical compositions were 82.2% of cellulose, 17.6% of xylan and 2.0% of lignin. In alkali prehydrolysis process, 96.9% of cellulose, 38.0% of xylan and 5.7% of lignin were remained. Enzymatic saccharification by commercial cellulases led to 87.0% of cellulose to glucose and 87.2% of xylan to xylose conversion. Produced glucose and xylose were fermented with fermenting yeast (Saccharomycess cerevisiae), which resulted in selective fermentation of glucose only to bio-ethanol. Residual monosaccharides after fermentation were consisted to 0.4-1.4% of glucose and 92.1-99.5% of xylose. Ethanol concentration was highest for 24 h fermentation as 57.2 g/L, but gradually decreased to 56.2 g/L for 48 h fermentation and 54.3 g/L for 72 h fermentation, due to the ethanol consumption by fermenting yeast.

• Korean Journal of Microbiology
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• v.15 no.3
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• pp.113-121
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• 1977

The 685 strains of Korean Sspergilli are isolated, cultured purely, and their Avicelase, CMCase and Salicinase activities are measured, in order to select the best strains exhibiting predominant cellulase activities, and to survey their cellulase activities in taxonomical and ecological viewpoints. Strains No.175, 255 and 254 are selected as the best Avicelase producing strains, strains No.131, 151 and 116 are selected as the best CMCase producing strains, and strains nO.456, 457 and 253 are selected as the best Salicinase producing strains. A niger group and A, clavatus group exhibited the highest activities of Avcelase and Salicinase, respectively. A.iniger group and A, clavatus groups are showed the highest activites of CMCase. Among the different species tested, the activities of Avicelase, CMCase andSalicinase are highest in A.phoenicis, A.clavatus, and A. japonicus and A.giganteus, respectively. Cellulase activities of Aspergilli from the inland regions of Korea are higher, more or less, than those of the strains from the other regions. Avicelase and CMCase activities of Aspergilli isolated from bread and Korean cake are relatively higher, and Salicinase activities of the strains isolated from the cereals are higher than those of the strains from the other habitat substrate.

• Journal of the Korean Home Economics Association
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• v.39 no.4
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• pp.49-59
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• 2001

Natural red and yellow dyestuff was extracted from safflower (Carthamus tinctorius Linnaeus) by a new process of cellulase extraction compared with the conventional hot water extraction. Dyestuffs were extracted from safflower easily and repeatedly by means of cellulose as safflower cell wall destroyer. It means that new dyestuff extraction by cellulase improves not only yields of dyestuff from safflower successfully but also the rate of repetition of extraction. From the above experiments, the conclusions of this study were summarized as follows. 1. The optimum conditions of dyestuff extraction from safflower by general extraction method were that the solvent was the water of pH 6.0 on yellow dyestuff and 3% $K_2CO_3$ solution on red dyestuff, extraction temperature was $55^{\circ}C$, and extraction time was 30 min. 2. Among various cellulase, the NOVO cellulase was the best cell wall destroyer of safflower and finally produced the largest amount of dyestuff from safflower by cellulase extraction method. 3. The optimum conditions of dyestuff extraction by cellulase extraction method were conducted on 10 unit of cellulase per gram of safflower at $100m{\ell}$ water of pH 5.0 at $50^{\circ}C$ for 30 min.

• Journal of the Korean Society of Clothing and Textiles
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• v.25 no.1
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• pp.144-153
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• 2001

The effect of crosslinking on hand of the cellulase treated Tencel fabrics was investigated. Tencel fabrics were crosslinked with DMDHEU, mechanically prefibrillated, hydrolyzed by cellulase, and treated with softener. The treated fabrics were characterized by add-on, weight loss, DP rating, WRA, strength, SEM analysis and hand measurement. As DMDHEU concentration increased, weight loss of DMDHEU/cellulase treated fabrics decreased. However, cellulase treatment decreased DP properties and strength retention. Less fibrils were observed in the cellulase treated fabrics after DMDHEU treatment than the cellulase treated ones. It was confirmed that crosslinking with DMDHEU treatment was effective to control fibrillation. At 5% of DMDHEU concentration, DMDHEU/cellulase treated fabrics showed softer, smoother and bulkier hand compared with other treated fabrics. Among mechanical properties, bending and shearing properties were decreased progressively through DMDHEU, cellulase, and softener treatment. DMDHEU treatment contributed to impart resilience, cellulase treatment to bulkiness and softener treatment to smoothness. As the treatment of DMDHEU, cellulase, and softener progressed NUMREI, FUKURAMI, and THV increased with the exception of KOSHI.

• Journal of the Korean Society of Clothing and Textiles
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• v.22 no.8
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• pp.1141-1149
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• 1998

Tencel fabrics were treated with NaOH, mechanically prefibrillated, and hydrolyzed by cellulase. Softner was applied to improve hand of the treated Tencel fabrics after prefibrillation and cellulase treatment. Kawabata's Evaluation System for Fabrics(KES-FB) was used to evaluate effects of NaOH pretreatment, prebifrillation, and cellulase and softner treatments on fabric hand of the treated fabrics. Primary hand values of women's medium thick fabrics such as KOSHI, NUMERI, FUKURAMI, and SOFUTOSA, and total hand values were evaluation parameters. As the treatments of prefibrillation, cellulase, and softner progressed, values in bending and shearing properties decreased and softness and elasticity were imparted to the treated fabrics. Specifically, compressional linearity, compressional energy, and thickness of the treated fabrics increased by prefibrillation, providing bulkiness to the treated fabrics. Values indicating surface properties increased owing to fibrils formed by prefibrillation treatment, but removal of fibrils by cellulase treatment enhanced smoothness. As the fabrics were exposed to various treatments such as NaOH pretreatments, prefibrillation, and cellulase and softner treatments, NUMERI, FUKURAMI, SOFUTOSA, and total hand values increased with the exception of KOSHI, Consequently, the treated fabrics became softer, smoother, and more elastic. Especially, the NaOH pretreatment provided superior SOFUTOSA to Tencel fabrics.

• KSBB Journal
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• v.29 no.4
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• pp.239-243
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• 2014

Recently, there is a growing interest in efficient biomass pretreatment and saccharification processes to produce biofuels and biochemicals from renewable non-food biomass resources. In this study, glucose was produced from cellulose by immobilizing cellulase enzyme on chitosan beads which was reported to have high pH and temperature stability. The immobilized amounts of cellulase on chitosan beads linearly increased with increasing the concentrations of cellulase solution. The glucose production increased to 7.2 g/L from 1% carboxymethyl cellulose (CMC) substrate when immobilized at 20% cellulase solution. The maximum specific activity was 0.37 unit/mg protein when immobilized at 8% cellulase solution. At pH 7 and $37^{\circ}C$, the optimum reaction composition was 0.5 g beads/L from 1% CMC substrate. At this condition, the conversion to glucose completed at ca. 20 min.

• Textile Coloration and Finishing
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• v.5 no.4
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• pp.10-19
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• 1993

Broad, mercerized broad, mercerized twill cotton fabrics and rayon fabric were treated with cellulase for 30~480 minutes at different temperature, concentration, time, and also treating methods such as continuously-treated or repeatedly-treated, and dyed with two direct dyes before or after enzyme treatment. From the experimental result by treating under the various conditions above, it was obtained that the weight loss increased more in thin fabric than thick one. In addition, it was considered that the treatment in 5$0^{\circ}C$ for 240 minutes brought about the ideal weight loss and flexible hand of the specimens. For both broad and mercerized broad, repeated treatment showed more weight loss than continuous. Direct dye on cotton fabric apparently inhibited hydrolysis. Ionic surfactants showed the inhibition effect of the catalytic hydrolysis of enzyme, on the other hand, nonionic surfactant did not.