• Title/Summary/Keyword: 세포 변형

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Study of Trend of Reporting on GMO as a Convergence Technology: Focused on analyzing Articles in Major Domestic Daily Newspapers from 1994 to 2015 (융복합기술로서 GMO에 관한 보도경향연구: 1994~2015년까지 국내 주요일간지 기사분석을 중심으로)

  • Cho, Hang-Min
    • Journal of Digital Convergence
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    • v.14 no.12
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    • pp.267-281
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    • 2016
  • This study analyzed the contents of articles on GMO in Chosun Ilbo and Hankyoreh from 1994 through 2015 to check the trend of reporting on GMO as a science and technology risk issue. As a result, 'risk' and 'anxiety/concern' were continuously treated as important subjects, and there was a lack of depth and journalist's professionalism in all three periods: 'Introduction of GMO technology (1994-2000)', 'Development of GMO technology (2001-2010)' and 'Social acceptance of GMO (2011-2015).' In a comparison between the media with different ideological goals, the Chosun Ilbo had a stronger new-technological inclination to GMO than the Hankyoreh in reporting topics and tones of arguments. As a result of checking the relation of the tones in reporting with the 'risk-benefit' of GMO, reports in negative tones underlined 'risk' while those in positive tones, 'benefit.' This study could suggest improvements of subject bias and unprofessionalism in the domestic science journalism, the barometer of public awareness of disputes over science and technology risk.

Enhancing Production Rate of Emulsion via Parallelization of Flow-Focusing Generators (유동-집속 생성기의 병렬화를 통한 에멀젼 생산속도 향상)

  • Jeong, Heon-Ho
    • Korean Chemical Engineering Research
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    • v.56 no.5
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    • pp.761-766
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    • 2018
  • Droplet-based microfluidic device has led to transformational new approaches in various applications including materials synthesis and high-throughput screening. However, efforts are required to enhance the production rate to industrial scale because of low production rate in a single droplet generator. In here, we present a method for enhancing production rate of monodisperse droplets via parallelization of flow-focusing generators. For this, we fabricated a three-dimensional monolithic elastomer device (3D MED) that has the 3D channel structures in a single layer, using a double-sided imprinting method. We demonstrated that the production rate of monodisperse droplet is increased by controlling the flow rate of continuous and dispersed phases in 3D MED with 8 droplet generators. Thus, we anticipate that this microfluidic system will be used in wide area including microparticle synthesis and screening system via encapsulation of various materials and cells in monodisperse droplets.

Determination of the Structure of 5S rRNA from Xanthomonas citri (Xanthomonas citri의 5S rRNA 의 구조 결정)

  • Bongrae Cho;Myung-Un Choi;Se Won Seh;ahei Ihm;Moonjoo Koh;Inwon Park
    • Journal of the Korean Chemical Society
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    • v.36 no.3
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    • pp.460-465
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    • 1992
  • The structure of th 5S rRNA isolated from Xanthomonas citri was determined by enzymatic and chemical degradation methods. It consists of 119 nucleotides and contains no modified nucleosides. As does the 5S rRNA of X. maltophilia, it contains an additional uridine residue on the 5'-terminus. The secondary structure of the 5S rRNA from X. citri was almost identical to the generalized models proposed by many other workers [De Wachter et al., Biochimie, 64, 311 (1982); Specht et al., Nucleic Acids Res., 18, 2215 (1990); Cho et al., Proceedings of the First Symposium on Biomolecules, p. 9 (1991)]. The secondary structure of 5S rRNA from X. citri consists of five helices, five loops and two bulges. This 5S rRNA has a uridine residue at position 66 as a bulge.

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Morphological Characteristics of Chlamydospores of Cylindrocarpon destructans Causing Root-rot of Panax ginseng (인삼 뿌리썩음병균 Cylindrocarpon destructans 후막포자의 형태적 특성)

  • Cho, Dae-Hui;Yu, Yun-Hyun;Kim, Young-Ho
    • Journal of Ginseng Research
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    • v.27 no.4
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    • pp.195-201
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    • 2003
  • Chlamydospore formation from mycelia and conidia of Cylindrocarpon destructans isolated from the root rot lesion of the Panax ginseng was investigated by scanning electron and light microscopy. Typical chlamydospores were formed only from hyphae but not from conidia on culture media. However, immature chlamydopspore-like cells were formed from microconidia after 12 days of incubation at 20$^{\circ}C$ on Czapek Dox broth (CDB) adjusted to pH 4.0. Chlamydospores were yellowish or reddish brown in color, and produced singly or in chain with the hyphal intercalary or terminal position on potato-dextrose agar, V-8 juice agar and CDB with no addition of nitrogen sources after 16∼20 days of incubation at 20$^{\circ}C$. They were 11.3 to 11.9 $\mu\textrm{m}$ in diameter, having many lumps-like warts on their surface with the length of 1.5 to 1.8 $\mu\textrm{m}$.

Radioprotective Effects of Propolis on the Mouse Testis Exposed to X-ray. (프로폴리스가 X-선에 노출된 마우스 정소에 미치는 방사선 방어 효과)

  • Ji, Tae-Jung;Kim, Jong-Sik;Jeong, Hyung-Jin;Seo, Eul-Won
    • Journal of Life Science
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    • v.17 no.5 s.85
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    • pp.664-670
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    • 2007
  • The propolis is natural product produced by honeybees and is known to have many biologically useful properties such as anti-microbial, anti-oxidative and anti-tumorigenic activity. However, its radio-protective property has not been well studied. To investigate radio-protective effect of propolis on mouse testis, mice were supplemented with propolis after 5 Gy irradiation. The histological changes of testis were detected by TEM. The results indicate that propolis may protect tissue deformation which is induced by 5 Gy of ionizing radiation. Furthermore, to elucidate the potential molecular mechanisms involved in radio-protective property of propolis, we performed microarray experiments using oligo DNA microarray. We found 65 up-regulated genes and 224 down-regulated genes, whose expression levels were affected more than 2-fold by propolis treatment in mice irradiated at 5 Gy. We confirmed microarray data with reverse transcription-PCR using gene specific primers. The results of RT-PCR are highly correlated with those of microarray. These results may help understanding molecular mechanisms of radioprotective effects by propolis in mouse model.

Recent Advances in 3D/4D Printed Electronics and Biomedical Applications (3D/4D 프린트된 전자기기 및 바이오메디컬 응용기술의 최근 발전)

  • Hyojun Lee;Daehoon Han
    • Journal of the Microelectronics and Packaging Society
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    • v.30 no.4
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    • pp.1-7
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    • 2023
  • The ability of 3D/4D printing technology to create arbitrary 3D structures provides a greater degree of freedom in the design of printed structures. This capability has influenced the field of electronics and biomedical applications by enabling the trends of device miniaturization, customization, and personalization. Here, the current state-of-the-art knowledge of 3D printed electronics and biomedical applications with the unique and unusual properties enabled by 3D/4D printing is reviewed. Specifically, the review encompasses emerging areas involving recyclable and degradable electronics, metamaterial-based pressure sensor, fully printed portable photodetector, biocompatible and high-strength teeth, bioinspired microneedle, and transformable tube array for 3D cell culture and histology.

THE EFFECTS OF DRYING AGENTS AND BONDING AGENTS ON THE SHEAR BOND STRENGTH OF SEALANTS TO ENAMEL (치면건조제와 접착제의 사용에 따른 치면열구전색재의 전단결합강도에 관한 연구)

  • Lim, Hyun-Hwa;Jang, Ki-Taek;Kim, Chong-Chul;Hahn, Se-Hyun
    • Journal of the korean academy of Pediatric Dentistry
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    • v.30 no.2
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    • pp.196-203
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    • 2003
  • The application of sealants is a highly technique-sensitive procedure, requiring an extremely dry field prior to placement. Moisture contamination of the etched enamel surface before sealant placement is cited as the main reason for sealant failure. The purpose of this study was to evaluate the effects of different methods of sealant application on the shear bond strength of sealants to enamel. In groups 1, 2, 3, 4 Teethmate(unfilled sealant) was used, while Ultraseal XTplus(filled sealant) was used in groups 5, 6, 7, 8. Groups 1 and 5(control) were acid etched for 15 seconds using 35% phosphoric acid, washed and then dried. In groups 2, 6 drying agents were applied, and in groups 3, 7 bonding agents were applied and light cured. In groups 4 and 8 both drying agent and bonding agent were applied. Then sealant was cured to the specimen using molds 3mm in diameter and 2mm in height. Thermocycling was performed and shear bond strength was finally measured. The following results were obtained : 1. Groups using filled sealant(groups 5, 6, 7, 8) showed higher shear bond strengths compared to groups using unfilled sealant(groups 1, 2, 3, 4). 2. Among groups using unfilled sealant(groups 1, 2, 3, 4), groups 2, 3, 4 showed significantly higher shear bond strength compared to group 1(p<0.05). There were no significant differences among groups 2, 3 and 4. 3. There were no significant differences(p>0.05) among groups using filled sealant(groups 5, 6, 7, 8). 4. When modes of fracture were examined, cohesive failure was observed in groups 2, 3 and 4.

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Radiotherapy for Oral Cavity Cancer (구강암의 방사선치료)

  • Shim Jae Won;Yoo Seong Yul;Koh Kyoung Hwan;Cho Chul Koo;Yun Hyong Geun;Kim Jae Young
    • Radiation Oncology Journal
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    • v.11 no.2
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    • pp.267-275
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    • 1993
  • Eighty five patients of oral cavity cancer, treated with radiation at the Department of Therapeutic Radiology, Korea Cancer Center Hospital, during the period from March 1985 to September 1990 were analyzed retrospectively. Among 85 patients, 37 patients were treated with radiation only and 48 patients were treated with radiation following surgery. And 70 patients received external irradiation only by $^{60}Co$ with or without electron, the others were 7 patients for external irradiation plus interstitial implantation and 8 patients for external irradiation plus oral cone electron therapy. Primary sites were mobile tongue for 40 patients, mouth floor for 17 patients, palate for 12 patients, gingiva including retromolar trigone for 10 patients, buccal mucosa for 5 patients, and lip for 1 patient. According to pathologic classification, squamous cell carcinoma was the most common (77 patients). According to AJC TNM stage, stage I + II were 28 patients and stage III+IV were 57 patients. Acturial overall survival rate at 3 years was $43.9\%,$ 3 year survival rates were $60.9\%$ for stage I + II, and $23.1\%$ for stage III+IV, respectively. As a prognostic factor, primary T stage was a significant factor (p<0.01). The others, age, location, lymph node metastasis, surgery, radiation dose, and cell differentiation were not statistically significant. Among those factors, radiation plus surgery was more effective than radiation only in T3+T4 or in any N stage although it was not statistically sufficient (p<0.1). From those results, it was conclusive that definitive radiotherapy was more effective than surgery especially In the view of pertainig of anatomical integrity and function in early stage, and radiation plus surgery was considered to be better therapeutic tool in advanced stage.

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Comparison of Distribution of Cadmium-109 and Gallium-67 in Sarcoma-Bearing Mice (Sarcoma-bearing Mice에서 Cadmium-109과 Gallium-67의 체내 분포의 비교)

  • Sohn, Myung-Hee;Chang, Sook-Kyeong;Chung, Kyung-Ho;Han, Young-Min;Kim, Chong-Soo;Choi, Ki-Chul;Yim, Chang-Yeol;Kang, Shin-Hwa
    • The Korean Journal of Nuclear Medicine
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    • v.28 no.1
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    • pp.98-105
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    • 1994
  • The search for tumor-avid agents for use in nuclear medicine imaging or therapy is a field of ongoing importance. Metallothionein (MT) is an intracellular protein that binds many metals with isotopes having imaging or radiotherapeutic potential. The purpose of the study was to determine whether uptake of radioisotopes that bind to MT is increased in tumor. We measured the uptake of Cd-109 and Ga-67 in tumor and normal tissues of sarcoma-bearing mice. Tumors were grown subcutaneously in female Balb/C mice from cultured Balb/3T3 cells transformed by the Moloney murine sarcoma virus (MMSV). When the tumors reached about 1 cm in diameter, mice were injected subcutaneously with Cd-109 and Ga-67. Eighteen and seventy-two hours later, the mice were sacrified. Organs and tissues were removed, weighed, and activity per mg tissue determined by gamma well-counting. Uptake of Cd-109 by MMSV tumors exceeded that by normal tissues examined, with the exception of liver and kidney (the organs known to be richest in MT). The tumor-to-tissue ratios of uptake for Cd-109 were far greater than those for Ga-67 for many normal tissues of great importance in terms of background activity (bone, intestine, fat, muscle, and blood). We concluded that metals that bind to MT may be useful for oncologic imaging or rediotherapy of cancer.

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Effect of Paroxetine and Sertraline Treatment on Forced Swim Test-Induced Behavioral and Immune Changes in the Mouse (마우스 강제수영에 의한 행동 및 면역반응 변화에 대한 Paroxetine과 Sertraline의 효과)

  • Eum, Se-Yeun;Jeong, Min-Ho;Lim, Young-Jin;Kim, Bu-Kyung;Jeong, Soo-Jin;Hahn, Hong-Moo;Choe, Byeong-Moo
    • Korean Journal of Psychosomatic Medicine
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    • v.8 no.1
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    • pp.46-57
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    • 2000
  • Objectives : The purpose of the present study was to examine the effect of subacute treatment with the selective serotonin reuptake inhibitors(paroxetine and sertraline) on immobility in the forced swim test(FST) and on FST-induced changes in immune parameters of the mice. Methods : Authors applied a modified method of FST by Porsolt et al. Over 5 BALB/c mice were used for each group of experiments. To explore the changes in immune parameters by FST, authors investigated the production of anti-rat RBC antibody, concanavalin A(ConA)- or lipopolysaccharide(LPS)-stimulated splenocytes proliferation assay and cytokine gene expression. Results : Both paroxetine and sertraline decreased the duration of immobility in a dose-related manner. FST-performed mice showed a significant decrease in mitogenic responses of splenocytes and a slight increasing tendency in anti-rat RBC antibody response. All these responses were attenuated significantly by paroxetine and attenuated nearly nominal significance level by sertraline. The cytokine profiles of ConA-stimulated splenocytes from FST-performed mice showed stronger expression of IL-4 and weaker expression of IL-2 than control mice, and no changes in the expressions of IFN-$\gamma$ and lymphotoxin. IL-6 and IL-10 were not expressed in both group of mice. The pretreatment of paroxetine and sertraline attenuated the altered cytokine expressions in FST-performed mice to some extent. Some alterations of the expressions of IL-6 and IL-10 were observed in the mice which the selective serotonin reuptake inhibitors had been pretreated. Conclusion : The subacute treatment of paroxetine and sertraline attenuated the FST-induced behavioral and immune changes, and these serotonin reuptake inhibitors may exert some modulating effects on the immune system by the induction of cytokine gene expression, especially IL-6 and IL-10.

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