The Korean Journal of Nuclear Medicine (대한핵의학회지)
- Volume 28 Issue 1
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- Pages.98-105
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- 1994
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- 1225-6714(pISSN)
Comparison of Distribution of Cadmium-109 and Gallium-67 in Sarcoma-Bearing Mice
Sarcoma-bearing Mice에서 Cadmium-109과 Gallium-67의 체내 분포의 비교
- Sohn, Myung-Hee (Department of Radiology, Chonbuk National University Medical School) ;
- Chang, Sook-Kyeong (Department of Radiology, Chonbuk National University Medical School) ;
- Chung, Kyung-Ho (Department of Radiology, Chonbuk National University Medical School) ;
- Han, Young-Min (Department of Radiology, Chonbuk National University Medical School) ;
- Kim, Chong-Soo (Department of Radiology, Chonbuk National University Medical School) ;
- Choi, Ki-Chul (Department of Radiology, Chonbuk National University Medical School) ;
- Yim, Chang-Yeol (Department of Internal Medicine, Chonbuk National University Medical School) ;
- Kang, Shin-Hwa (Department of Radiology, Chonju WooSuk University Oriental Medical College)
- 손명희 (전북대학교 의과대학 방사선과학교실) ;
- 장숙경 (전북대학교 의과대학 방사선과학교실) ;
- 정경호 (전북대학교 의과대학 방사선과학교실) ;
- 한영민 (전북대학교 의과대학 방사선과학교실) ;
- 김종수 (전북대학교 의과대학 방사선과학교실) ;
- 최기철 (전북대학교 의과대학 방사선과학교실) ;
- 임창열 (전북대학교 의과대학 내과학교실) ;
- 강신화 (전주우석대학교 한의과대학 방사선과)
- Published : 1994.05.15
Abstract
The search for tumor-avid agents for use in nuclear medicine imaging or therapy is a field of ongoing importance. Metallothionein (MT) is an intracellular protein that binds many metals with isotopes having imaging or radiotherapeutic potential. The purpose of the study was to determine whether uptake of radioisotopes that bind to MT is increased in tumor. We measured the uptake of Cd-109 and Ga-67 in tumor and normal tissues of sarcoma-bearing mice. Tumors were grown subcutaneously in female Balb/C mice from cultured Balb/3T3 cells transformed by the Moloney murine sarcoma virus (MMSV). When the tumors reached about 1 cm in diameter, mice were injected subcutaneously with Cd-109 and Ga-67. Eighteen and seventy-two hours later, the mice were sacrified. Organs and tissues were removed, weighed, and activity per mg tissue determined by gamma well-counting. Uptake of Cd-109 by MMSV tumors exceeded that by normal tissues examined, with the exception of liver and kidney (the organs known to be richest in MT). The tumor-to-tissue ratios of uptake for Cd-109 were far greater than those for Ga-67 for many normal tissues of great importance in terms of background activity (bone, intestine, fat, muscle, and blood). We concluded that metals that bind to MT may be useful for oncologic imaging or rediotherapy of cancer.
Metallothionein (MT)는 세포내에 존재하는 cystein이 풍부한 적은 단백질로서 핵의학적 영상이나 치료에 이응이 가능할 여러 금속성 방사성 동위원소와 결합한다. 본 연구는 sarcoma를 주사한 Balb/C mice에서 MT와 결합하는 방사성동위원소인 cadmium이 종양에 축적되는가를 확인하고, 그것이 핵의학적인 영상과 치료에 이용될 수 있는지와 Ga-67을 대신할 수 있는지를 보기 위해 종양과 정상조직에서 Cd-109과 Ga-67의 섭취율을 비교하고 종양과 정상조직의 비를 비교하였다. Balb/3T3 세포를 Molony murine sarcoma virus (MMSV)로 변형시킨 세포를 Balb/C mice의 왼쪽 서혜부에 피하로 주사한 후 종양이