• Korean Chemical Engineering Research
• /
• v.62 no.3
• /
• pp.201-213
• /
• 2024

Cells first emerged 4 billion years ago and have evolved over a long period into an excellent system. Bottom-up synthetic biology is a research field that aims to develop "artificial cells" by returning to 4 billion years ago and redesigning cells from scratch. Although these artificial cells are not perfect, they are artificial cell mimicry systems that possess important characteristics of living cells. By designing the artificial cells, researchers in this field aim to explore the organization and the origins of cells from a different approach than traditional cell biology and ultimately seek to replace the use of living cells. This review aims to introduce the concepts and recent research in capsule and biocatalyst-based artificial cells, which have been actively studied recently.

• The Korean Journal of Zoology
• /
• v.23 no.2
• /
• pp.77-88
• /
• 1980

The ultrastructures of the mucous glands in the Asiatic land salamander (Hynobius leechi) skin were observed by means of electron microscope. The results were as follows; 1. The mucous gland of the Asiatic land salamander skin consisted of a body of gland and a duct. The body of gland consisted of the glandular epithelial and the myoepithelial cells. 2. The light, the dark, the mitochondria-rich, the endoplasmic reticulum-developed, the secretory granule-containing and the vesiculated cells were observed in the mucous glandular epithelium. 3. It is supposed that the light, the dark and the mitochondria-rich cells are undifferentiated cells, the endoplasmic reticulum-developed cell is pre-secretory cell, the secretory granule-containing cell is mature cell and the vesiculated cell is evacuated cell.

• Anatomy & Biological Anthropology
• /
• v.26 no.1
• /
• pp.41-49
• /
• 2013

Dental epithelial and mesenchymal cells that form the teeth undergo dynamic changes in cell cycle during tooth development and morphogenesis. Although proliferation has been known as a key event during odontogenesis, the cell cycle phases and their relations with the complicated molecular mechanisms of tooth development are not fully understood yet. This study comparatively examined the expression patterns of Ki-67, cyclin A, and cyclin D1 during tooth development in the mouse incisor and molar in order to identify the cell-cycle characteristics during odontogenesis. We found that Ki-67 and cyclin A were expressed in the proliferating cells in the dental epithelial and mesenchymal tissues at the bud, cap and bell stages. Cycln D1 showed distinct expression in the incisor odontoblast region and the enamel knot, in which Ki-67 nor cyclin A was expressed. Our results provide specific information on the cell cycle phases during tooth development that may provide clues to relate them with the complex odontogenic mechanisms. Furthermore, we suggest that our findings enlightened the previous studies on the incisor odontoblasts and the enamel knot during tooth development.

• The Journal of the Korean dental association
• /
• v.45 no.6 s.457
• /
• pp.370-376
• /
• 2007

"내 치아를 백옥 같이 예쁘게 보이도록 해 주세요!" 라는 환자들의 요청을 만족시키기 위해서 개원 치과의사들이 지난 30여년간 치과진료시에 전 세계적으로 이용하고 있는 과산화 수소가 포함된 각종 치아 미백제의독성에 대한 현실적인 문제점을 지적해 보면서 과학적인 견지에서 현대 분자생물학이나 세포생물학이 제공할 수 있는 해결방안을 제시하고져한다.

• The Korean Journal of Zoology
• /
• v.23 no.4
• /
• pp.219-228
• /
• 1980

The ultrastructures of the granular glands in the asiatic land salamander (Hynobius leechi) skin were observed by means of electron microsope The results were as follows; 1. The granular gland of the asiatic land salamander skin consisted of a body of gland and a duct. The body of the granular gland consisted of the glandular epithelial cells and the myoepithelial cells. The duct of the granular gland consisted of the keratinocytes. 2. The glandular epithelial cells in the asiatic land salamander skin were divided into the dark cell and the light cells in accordance with the electron density of the cytoplasm. 3. The secretory granules of the granular glands were round or oval in form and were divided into the various granules, showing the secretory granules showing weak electron density had the parts showing strong electron density near the granular membrane. 4. It is supposed that showing the different electron densities of the granules in a glandular epithelial cell is due to different mature stages and to different level of water absorption, and the chemical components of the granules showing different electron densities are identical.

• The Microorganisms and Industry
• /
• v.17 no.1
• /
• pp.38-50
• /
• 1991

Flow cytometer(유식세포분석분리기:FCM)는 부유되어 있는 세포의 여러 특성을 측정할 수 있도록 고안되었기 때문에 동물의 체내에 있는 세포중 자연적으로 부유되어 있는 혈구세포를 분석하기가 가장 용이하다. 면역계에서 가장 중추적인 역할을 담당하며 면역반응에 특이성과 기억능력 등을 부여하는 림프구와 탐색작용을 가장 왕성히 나타내는 과립구나 단구 등이 혈액내에 존재하기 때문에 FCM을 이용하여 개체의 방어기능을 맡고 있는 면역세포를 분석하는 일이 가장 먼저 활발히 이루어졌음은 당연하였다. 도한 1970년대 면역학이 큰 발전에 이룩한 단크론항체가 Miller와 Kohler에 의하여 개발되었기 때문에 면역세포의 분화항원에 대한 여러 종류의 단크론항체는 FCM의 이용으로 면역세포를 여러 아형으로 분석하고 분리가 가능하게 하였다. 마지막으로 FCM이 최근에 개발된 것에 맞추어 세포성 면역학이 30년 전부터 급속도로 연구되었으므로 면역학적 연구가 FCM의 이용으로 더욱 활성화되었음은 주지의 사실이다. 이런 시점에서 저자는 FCM의 원리와 면역생물학에서의 FCM의 역할에 대하여 간단히 소개하려고 한다.

• The Korean Journal of Zoology
• /
• v.21 no.1
• /
• pp.29-39
• /
• 1978

The authors observed the ultrastructure of the mucous glandular epithelial cells in the amphibian skin by mean of electron microscope. The specimens from the experimental animals were fixed in 2.5% glutaraldehyde-oaraformaldehyde fixative in phosphate buffer at pH 7.2 prior to fixation in 1% osmium tetroxide, dehydrated in graded ethanol and acetone, embedded in Epon 812 mixture, and sectioned with Sorvall MT-2 ultramicrotome. The ultrasections were contrasted with uranyl acetate and lead citrate and observed with a JEOL-100B electron microscope. The results were as follows: 1. The cutaneous mucous glands in amphibia consisted of the glandular epithelial and the myoepithelial cells. 2. Several different cells in ultrastructure were observed in the mucous glandular epithelium of the adult amphibian skin. a. The dark and the light cells were observed in Hynobius leechi. b. The mitochondria-rich and the round secretory granule-containing cells were observed in Bombina orientalis. c. The round secretory granule-containing and the foam-like granule mass-containing cells were observed in Kaloula tornieri. d. The cutaneous mucous gland of Rana nigromaculata were divided into two types: A and B-type glands. In the A-type mucous gland, the mitochondria-rich and the round secretory granule-containing cells and in the B-type mucous gland, the mitochondria-rich, the secretory granule-containing and the ER-rich cells were observed. 3. Based upon the above findings, the authors infer that the mucous granular epithelium of the amphibian skin consists of the mitochondria-rich undifferentiated, the secetory granule-containing and mature, and the ER-rich evacuated cells.

• Optical Science and Technology
• /
• v.17 no.3
• /
• pp.22-28
• /
• 2013

• 대한치주과학회:학술대회논문집
• /
• 2007.11a
• /
• pp.25-26
• /
• 2007

• Journal of the KSME
• /
• v.53 no.5
• /
• pp.45-50
• /
• 2013

인체장기칩(Human Organs-on-Chips)은 미세공학, 생체모방, 세포생물학 분야의 기술을 시너지 있게 접목하여 개발되는 신개념의 인체 대리모델이다. 이 글에서는, 이러한 생체모사 시스템의 개발 배경과 최근 연구동향, 그리고 잠재성에 대해서 살펴본다.