• The Journal of the Korean Society for Microbiology
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• v.11 no.1
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• pp.1-11
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• 1976

An electron microscopic study has been made of the effects of change of cell structure of Escherichia coli treated with several disinfectants. The alterations observed as follows: 1. Nucleoid, cytoplasm which contain ribosomes and cell wall appeared to be composed of a parallel triple. layered membrane can observed in control Escherichia coli. 2. The nuclear material was no longer demonstrable in its normal sites. The cytoplasm lost its granularity, became homogeneous and disruption of cell wall were observed by the treatment with 70% ethyl alcohol and 3% $H_2O_2$. 3. Aggregation of ribosomes and condensation of nuclear material were also observed by the treatment of 5% lysol solution and 1% dodecyl-diamino-ethyl-glycin-hydrochloride. 4. In autoclaved group, the each layer of cell wall was separated and destroyed in some sites where cytoplasm was extracted.

• The Korean Journal of Zoology
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• v.34 no.3
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• pp.368-381
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• 1991

한국산 육생민달팽이유(Limaxpouus와 상ciluria hhstoferi)의 체강벽을 배측부, 복측부 및 외투천 등 3부분으로 나누어 관찰하였으며, 그 결과는 다음과 같다. 1, Limaxpouus의 배측부 체강벽은 많은 모지상돌기 형 태의 근조직과 체강벽상피세포로 구성되어 있으나, 복측부 체강조은 많은 수의 중성점액질 형성세포와 근섬유들로만 형성되었다. 2. Incilaria hhstoferi의 배측부와 복측부는 공히 중성점액질 형성세포와 근섬유들로만 형성되고, 체강상피세포는 관찰되지 않았다. 3. Incilorlo jruhstor$\ulcorner$sri의 병투막측 체준벽은 불규칙한 모양의 단층 체강상피세포와 많은 근섬유로 구성되었다.

• The Korean Journal of Mycology
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• v.38 no.1
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• pp.29-33
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• 2010

KGD1 gene was cloned by functional complementation of defects in $\beta$-1,3-glucan synthase activity of the previously isolated Saccharomyces cerevisiae mutant LP0353, which displays a number of cell wall defects at restrictive temperature. We performed the gene disruption experiment to characterize the function of KGD1 gene, which encodes $\beta$-ketoglutarate dehydrogenase, in cell wall biosynthesis. The disruption of KGD1 showed the decreased growth rate, the increase of chitin synthases activity, alterations in cell wall composition, and increase of susceptibility to cell wall inhibitors such as Calcofluor white and Nikkomycin Z. These results suggested that KGD1 might be involved in cell wall biogenesis, especially the biosynthesis of $\beta$-1,6-glucan and chitin in S. cerevisaie.

• Korean Journal of Food Science and Technology
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• v.36 no.3
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• pp.484-489
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• 2004

Bifidobacterium species isolated from infant feces were fractionated into cell wall, cytosol, and extracellular preparations of culture broth, and each fraction was examined for Peyer's patch-mediated bone marrow cell proliferation activity in vitro. Cell wall preparation of B. bifidum SL-21 (CWP) showed the highest bone marrow cell proliferating activity dose dependently, and enhanced production of cytokines, such as hematopoietic growth factor (GM-CSF), IL-2, and IL-6, in culture supernatant of Peyer's patch cells, After treatment with lysozyme, CWP was fractionated, among which intermediate molecular-weight fraction (30-50 kDa) showed significantly high bone marrow cell proliferating activity. These results suggest CWP of B. bifidum SL-21 effectively activates lymphocytes in Peyer's patch, and several cytokines, possibly playing important role in enhancement of systemic immune system, were produced by activated lymphocytes.

• Clean Technology
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• v.21 no.2
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• pp.90-95
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• 2015

Digital microfluidic electroporation system was used for the transformation of microalgae and we have obtained higher transformation efficiency and viability than that of conventional method. Key parameters of electroporation such as pulse voltage, number, and duration time were systematically investigated for two different microalgal strains with and without cell wall. We have found that cell wall does not always have negative effects on the gene transformation of microalgae. Parallel processing of proposed digital microfluidic electroporation was demonstrated together with on chip culture of microalgae.

• Journal of the Korean Wood Science and Technology
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• v.34 no.4
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• pp.9-14
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• 2006

This study was carried out to investigate the distribution and penetration of PEG molecules into the cell wall of wood by SEM-EDS method. SEM observation indicated that the cell walls of PEG-treated specimen were swollen, and didn't show any change in their shapes, while the cells of untreated specimen were more or less collapsed. The results of X-ray analyses of potassium stained samples revealed the distribution of the impregnated chemicals within the cell walls, when considering the distribution of potassium ions in the cell walls. Consequently, this study supported the possibility of PEG molecules penetrated into the wood cell wall play an important role in enhancing the dimensional stability of wood.

• Korean Journal of Plant Tissue Culture
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• v.27 no.6
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• pp.429-434
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• 2000

Isolated protoplasts from ginseng (Panax ginseng C. A. Meyer) callus tissue were cultured in modified MS media supplemented with various concentrations of dimethylsulfoxide (DMSO). The cell wall regeneration rate and cell division efficiency of the protoplasts were increased significantly by 1% DMSO treatment. However, there was no difference in the viability of protoplasts between the DMSO treatment and non-treatment. Transmission electron microscopy revealed that the microtubules were oriented in parallel manner to the plasmalemma after 3 days of culture in medium with 1% DMSO. Further, interconnected cellulose microfibrils were observed on the outer surface of the 3-day-cultured protoplasts by scanning electron microscopy These structures shown by electron microscopy were not observed in protoplasts cultured on DMSO-free media. This studies indicates that DMSO supplemented in culture media seemed to stimulate the cell wall regeneration and cell divisions of protoplasts by forming microtubule organizing centers (MTOC).

• Proceedings of the Korean Society of Postharvest Science and Technology of Agricultural Products Conference
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• 1995.06a
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• pp.25-25
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• 1995

• 한국생물공학회:학술대회논문집
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• 2001.11a
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• pp.479-482
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• 2001

Suspension cells of Angelica gigas Nakai was cultivated to produce extracellular polysaccharide(ECP) as immunostimulating agents. In this study, effects of pectinolytic enzymes such as pectinase. Pectinex on cell growth and ECPs production were investigated in suspension cultures. Optimum concentration of pectinase for ECP production ,vas found to be 0.1 U/L. at which ECP production was increased 1.39 fold (0.97 g/L) compared to control culture.

• Journal of Korea Technical Association of The Pulp and Paper Industry
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• v.29 no.1
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• pp.36-42
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• 1997

The fiber wall filling(FWF) technology, which is based on Precipitatin of fillers in the micropores of the cell wall structure of never-dried chemical pulp fiber, has been developed to improve filling and loading process in papermaking. In presenting FWF technique here, micropores of pulp fiber are first impregnated with an ionic solution of water soluble salt and consecutively impregnated with the second salt solution. This procedure generates an insoluble precipitate within the micropores of cell wall by chemical interaction of these two ionic salt solutions This is the first attempts to use FWF technology for the quality of waste paper grade which is recycled in papermaking, even though this FWF technology has been impressively improved for never-dried chemical pulp in filling and loading process of papermaking. The precipitated amount of CaCO$_3$ and SrCO$_3$ reached 5-6% and 4-5% of the waste paper weight respectively, which was measured by ash content of the burned waste paper fiber. On the other way the precipitated amounts of those materials impregnated into never-dried chemical pulp fiber have reached 17-18% and 16-18% respectively. The micropore loading technique gives optical and physical properties to the handsheets formed with celt-wall-filled fibers which are better than those handsheet properties resulting from conventional loading. The papers made from the cell-wall-filled pulps are stronger than those with the customary location of filler between the fibers.