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In vitro Bone Marrow Cell Proliferation of Cell Wall Preparation from Bifidobacterium bifidum SL-21  

Shin, Myong-Sook (Department of Food Science and Biotechnology, Kyonggi University)
Yu, Kwang-Won (Department of Kimchi and Food Science, Chongju National College of Science and Technology)
Shin, Kwang-Soon (Department of Food Science and Biotechnology, Kyonggi University)
Lee, Ho (Department of Food Science and Biotechnology, Kyonggi University)
Publication Information
Korean Journal of Food Science and Technology / v.36, no.3, 2004 , pp. 484-489 More about this Journal
Abstract
Bifidobacterium species isolated from infant feces were fractionated into cell wall, cytosol, and extracellular preparations of culture broth, and each fraction was examined for Peyer's patch-mediated bone marrow cell proliferation activity in vitro. Cell wall preparation of B. bifidum SL-21 (CWP) showed the highest bone marrow cell proliferating activity dose dependently, and enhanced production of cytokines, such as hematopoietic growth factor (GM-CSF), IL-2, and IL-6, in culture supernatant of Peyer's patch cells, After treatment with lysozyme, CWP was fractionated, among which intermediate molecular-weight fraction (30-50 kDa) showed significantly high bone marrow cell proliferating activity. These results suggest CWP of B. bifidum SL-21 effectively activates lymphocytes in Peyer's patch, and several cytokines, possibly playing important role in enhancement of systemic immune system, were produced by activated lymphocytes.
Keywords
Bifidobacterium bifidum; bone marrow cell proliferation; Peyer's patch; cell wall preparation; immune system;
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