• Title/Summary/Keyword: 세포간극

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Ocular transport of hydrophilic drugs: Enhancement of the paracellular penetration across cornea and conjunctiva in the rabbit (수용성약물의 안점막 투과기전에 관한 연구: 토끼의 각막 및 결막 세포간극경로의 투과촉진)

  • Chung, Youn-Bok;Lyoo, Seen-Suk;Han, Kun
    • Journal of Pharmaceutical Investigation
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    • v.26 no.1
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    • pp.43-53
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    • 1996
  • The objective of this study was to determine whether 4-phenylazobezyloxycarbonyl-Pro-Leu-Gly-Pro-D-Arg (Pz-peptide), an enhancer of hydrophilic solute permeability in the intestine, could elevate the paracellular permeability of hydrophilic drugs across cornea and conjunctiva in the rabbit. The in-vitro penetration of hydrophilic drugs (mannitol, atenolol) and lipophilic drug (propranolol) across the rabbit cornea and conjunctiva was studied either in the presence or absence of 3 mM Pz-peptide. Drug penetration was evaluated using the modified Ussing chamber. The conjunctiva was more permeable than the cornea to all drugs. Pz-peptide showed enhanced effects on the drug transport across cornea and conjunctiva in a concentration dependent manner. Effects or ion transport inhibitor on the mannitol penetration were then investigated. Mannitol penetration was not changed by serosal addition of $100\;{\mu}M$ ouabain, suggesting that $Na^+/K^+$ ion tranporter was not involved in the Pz-peptide induced elevation of paracellular drug permeability. Furthermore, effects of Pz-peptide and EDTA on the transport of atenolol and propranolol into the ocular tissues or blood circulation after its administration into both eyes were investigated. EDTA showed enhanced effect on propranolol transport into the ocular tissues, but Pz-peptide did not show significant difference. Systemic absorption of propranolol by the addition of EDTA or Pz-peptide was not changed. On the other hand, EDTA and Pz-peptide elavated the atenolol transport into the ocular tissues. The transport of atenolol into the blood circulation was also enhanced by the addition of EDTA, but no effect was observed by the addition of Pz-peptide. The above findings suggest that Pz-peptide would be used as an paracellular pathway enahncer of hydrophilic drugs into the eye, without affecting the systemic absortion of topically applied opthalmic drugs.

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Effect of Plant Population on the Growth, Yield and Quality of Sohyang (Nicotiana tabaccum L.) (소향(Nicotiana tobaccum L.)의 재식밀도와 생육, 수량 및 품종)

  • Bae, S.K.;Lim, H.G.;Son, S.H.
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.26 no.2
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    • pp.207-211
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    • 1981
  • The effect of plant populations - 16,667, 14,285, 12,499 or 10,7l4/10a on the agronomic characteristics of an aromatic tobacco, Sohyang was investigated for two years. The growth and number of suckers produced decreased as the population increased. The leaf area index (L.A.I) was higher in thinner planting, but there was no significant difference in weight per unit leaf area between treatments. Yield was highest, 1 24 kg/ l0a, in 16,667 plantings per l0a, but quality was not different among densitites tested. Similar trends were observed in monetary value per l0a. Concentrations of total alkaloids and nitrogen were low in denser planting, but no significant difference was observed in total sugar. The results suggest that about 16,000/10a more would be optimum number of plants for Sohyang.

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Studies on the drought resistance of mulberry trees (상수의 내건성에 관한 연구)

  • 김문협
    • Journal of Sericultural and Entomological Science
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    • v.7
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    • pp.1-26
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    • 1967
  • In order to develop the standards for the measurement of drought resistance in mulberry trees (Morus genus) the varietal differences of drought resistance were measured for 30 mulberry varieties, and the relationships between the drought resistance and the histological and physiological characteristics of mulberry leaves were investigated. The results were summarized as follows; 1. It is reasonable to use the drought resistance ratio, expressed by D/D'*100, for the standard of drought resistance measurement for mulberry tree as a perennial tree crop. Where: D stands for growth amount(shoot length) in the plot of dry treatment, at the end of treatment. D' stands for an expected value of D which is expressed by B*C/A. Here, A is the growth amount of wet treatment plot at the beginning of treatment, B is the growth amount of dry treatment plot at the beginning of treatment, and C is the growth amount of wet treatment plot at the end of treatment. 2. The results obtained from the application of above formula showed that the varieties Cadaneo, Tahozosaeng, Yongchunchuwu, Kaeryang suban. and Kabsun were highly resistant to drought and the varieties Jukmok, Shipyung, Sobun, Kaeryangzosaeng shipmoonza and Chungagokyo were highly susceptible. 3. Among leaf tissues. the rate of inter-cellular space showed the highest relationship with drought resistance. The correlation coefficient calculated (r=0.4153) was highly significant. Other leaf tissues such as epidermis and palisade showed no significant correlations with drought resistance. 4. The size and density of stomata were correlated to drought resistance. That is: Correlation between drought resistance and size of stomata(length ${\times}$ width)......r= -0. 3253(signif. at 5%) density(No. of stomata/l$\textrm{mm}^2$......r= +0.5047(signif. at 1%)

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Change of the Amylase Secretion on the Rat Submandibular Gland in the Restraint Stress Condition (구속스트레스에 의한 백서 악하선의 Amylase 분비 변화)

  • Koo, Han-Mi;Au, Q-Schick;Chun, Yang-Hyun;Hong, Jung-Pyo
    • Journal of Oral Medicine and Pain
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    • v.32 no.1
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    • pp.57-67
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    • 2007
  • In currently, stress diseases are increased that present several sign and symptoms. Under stress condition, there are dry mouth, burning mouth syndrome, oral mucosa diseases and halitosis more frequently. Changing of salivary proportion is checked in almost patients with changing of function and structure in salivary gland. This study purpose are what effect stress does on salivary gland, and a-amylase on salivary gland. This study was resulted that 1. Under restraint stress, acinar cells are vacuolization and changing of intercellular spaces are separated, and peripheral tissues of duct are changed 2. Acinar cells were shrunk after 3 hours under restraint stress, intercellular space was separated after 6hours, peripheral tissues of duct started to change after 72 hours, and acinar cells and peripheral tissues of duct were all severely changed after 168hours. 3. In immunohistochemical study, amylase reaction was showed partially and irregularly after 3 hours, was getting little milder after 6 hours. And amylase reaction was gradually increased from the time of 12 hours after experiment up to the time of 48 hours after experiment. But after 168 hours, amylase appearance was diminished. According this result, emotional stress can change of salivary gland structure, and amylase secretion, the important digestive enzyme from salivary gland is changed and it is supposed to make digestive disorder and to make halitosis efficiency. So, we need to study about secretion of amylase.

Effects of CEPA on the Secretion of Lacquer and Bark Physiology of Lactree(Rhus verniciflua Stokes) (CEPA 처리(處理)가 옻나무의 칠액분비(漆液分泌) 및 수피생리(樹皮生理)에 미치는 영향(影響))

  • Choi, Tae Bong;Kim, Mahn Jo;Hyun, Jung Oh
    • Journal of Korean Society of Forest Science
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    • v.89 no.1
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    • pp.116-125
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    • 2000
  • This study was conducted to investigate the effects of CEPA(2-chloroethyl phosphonic acid) on bark physiology and anatomy of lactree(Rhus verniciflua Stokes). Sample trees of similar size and growth rate were selected from 7-year-old lactree plantation located in Hyengsung-kun, Kangwon-do. Lanolin pastes containing 0.1, 1, or 10% CEPA were put into the bark-removed hole made by corer(${\phi}1cm$) on the main stem at 1.2m above the ground on June 16, 1995. Five weeks after application of CEPA, bark thickness was markedly increased as a result of the increase in the amount of phloem and intercellular spaces, and correlated with the increased production of urushiol. By the application of 10% CEPA, bark thickness was increased approximately 2.5 times, and the urushiol content within bark was increased 2.8 times compared to that of untreated trees because CEPA stimulated the accumulation of urushiol within bark. Treatment of 10% CEPA also increased the size and the total number of secretory canals, and induced an increase in ray width. The phloem parenchyma cells of CEPA-treated trees were well-developed and closely packed with little intercellular space.

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Morphological Characteristic of the Rust Fungi, Uromyces truncicola, and Histological Changes in the Infected Host Tree, Sophora japonica (회화나무 녹병균(Uromyces truncicola)의 형태적 특징과 감염 기주의 조직해부학적 변화)

  • Gil, Hee-Young;Koo, Chang-Duck
    • Journal of Korean Society of Forest Science
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    • v.99 no.3
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    • pp.277-284
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    • 2010
  • rust fungus, Uromyces truncicola, infected stems, branches and leaves of Sophora japonica trees, and 78% of planted host depending on investigated sites. Teliospores of this fungus were reddish brown, global to oval and $29-37{\times}24-27\;{\mu}m$. The urediniospores were yellowish brown, elliptical to oval with spiny surface and $28-39{\times}19-22\;{\mu}m$. In the infected branches cork layers were thickened, ray parenchymatous cells were distorted, branched or combined and increased in numbers. While ray parenchymatous cells in healthy branches were one or two layers, the cells in the infected branches were three or more layers to shape fusiform. In the infected branches xylem tissues also distorted and yellowish pigments formed in the intercellular space. In the less infected trees the hairs on the branches were dense and long, and the thickness of upper epidermal cell walls were ca 23.3 ${\mu}m$, while in the highly infected trees the hairs were less dense and short, and the thickness of upper epidermal cell walls was ca 17.4 ${\mu}m$. We think individual host trees resistant to this fungus are present.

Anatomical Comparison of Compression, Opposite, and Lateral Woods in a Branch of Pinus parviflora S. et Z. (섬잣나무(Pinus parviflora S. et Z.) 지재의 압축이상재, 측면재, 대응재에 관한 해부학적 특성 비교)

  • Xu, Guang Zhu;Eom, Young Geun
    • Journal of the Korean Wood Science and Technology
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    • v.33 no.1 s.129
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    • pp.38-47
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    • 2005
  • Compression, lateral, and opposite woods in a branch of Pinus parviflora S. et Z. were described and compared in the qualitative anatomical aspects through light and scanning electron microscopy. Tracheid transition from earlywood to latewood in the compression wood appeared to be relatively more gradual than in the opposite or lateral wood. Growth ring width and proportion of latewood were thought to be greater in the compression wood than in the lateral or opposite wood. The latewood tracheids of compression wood in transverse surface were mostly round, differently from those of lateral and opposite woods with square to angular shapes. Also, intercellular spaces, helical cavities and checks, and slit-like pit apertures were observed only in the compression wood tracheids. Cross-field pitting in the compression wood appeared not to be used as diagnostic guide because of their severe alteration from normal fenestriform or window-like type to cupressoid to taxodioid types. In tangential surface, fusiform rays in the compression wood were wider but lower than those in the lateral wood or opposite wood. In conclusion, compression wood was different from lateral and opposite woods but lateral and opposite woods were almost identical in qualitative anatomical features.

Anatomical Studies on the Features of Rays in Compression Wood of Korean Red Pine(Pinus densiflora S. et Z.) (소나무(Pinus densiflora S. et Z.) 압축이상재(壓縮異常材)의 방사조직(放射組織) 특성(特性)에 관한 해부학적(解剖學的) 연구(硏究))

  • Chung, Youn Jib;Lee, Phil Woo
    • Journal of Korean Society of Forest Science
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    • v.78 no.2
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    • pp.119-131
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    • 1989
  • This experiment was executed to investigate and compare qualitative and quantitative anatomical features in compression wood, opposite wood, and side wood formed in a bent stem, a straight branch, and an exposed horizontal root of Korean red pine(Pinus densiflora S. et Z.). The respective four discs containing compression wood taken at 20cm interval both in stem and branch as well as a disc containing well developed compression wood from horizontal root were analyzed. Percentage of compression wood and eccentricity showed decreasing tendency with the increasing distance in height direction of stem and length direction of branch. The qualitative anatomical features of compression wood appeared to differ from those of side and opposite wood in very gradual tracheid transition from earlywood to latewood, roundish tracheid shape on cross surface, tracheid distortion at tip on radial surface, existence of intercellular space, and helical cavity in tracheid wall. And the differences in these qualitative features among the compression wood, opposite wood, and side wood became less intensive with the decreasing trends in percentage of compression wood and eccentricity. The quantitative anatomical features in compression wood also appeared to be wider in that respective widths of fusiform and uniseriate ray than those of opposite and side wood, but the heights of fusiform and uniseriate ray in compression wood were smaller than in opposite and side wood. The number of horizontal resin canal(fusiform ray) and uniseriate ray, however, showed no differences among the compression wood, opposite wood, and side wood. And the number of vertical resin canal in unit area, $4{\pi}mm^2$ of compression wood was fewer than that in opposite wood, whereas numerous vertical resin canals contained in a growth ring. These rays of compression wood seemed to be characterized by smaller height and wider width than those of opposite and side wood.

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Effects of Light, Temperature, Water Changes on Physiological Responses of Kalopanax pictus Leaves(V) - Physiological Responses by the Changes of Leaf Water Potential - (광, 온도, 수분 변화에 따른 음나무 엽의 생리반응(V) - 잎의 수분 포텐셜 변화에 따른 생리반응 -)

  • Han, Sang-Sup;Jeon, Doo-Sik;Sim, Joo-Suk;Jeon, Seong-Ryeol
    • Journal of Forest and Environmental Science
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    • v.22 no.1
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    • pp.76-82
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    • 2006
  • 1. This stuty was investigated in 6-year-old Kalopanax leaves in plantation forest. 2. The decrease in net photosynthesis rate began at -0.80 MPa water potential, and then approached zero at -2.50 MPa. The relative net photosynthesis rate which is 50% occurred at -1.70 MPa. The stomatal conductance increased temporarily until -1.00 MPa, and then rapidly decreased. At -2.50 MPa, the relative stomatal conductance was 7% of maximum value. 3. The stomatal transpiration rate increased temporarily until -1.00 MPa, and then rapidly decreased. At -2.50 MPa, the relative stomatal transpiration rate was about 17% of maximum rate. The water use efficiency rapidly decreased with decreasing water potential, and then approached about zero at -2.50 MPa. 4. With decreasing leaf water potential, the $CO_2$ content ratio. $C_i/C_a$ in intercellar rapidly increased. The vapor pressure deficit, VPD gradually increased until -2.00 MPa, and then rapidly increased.

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Characteristics of Adhesive Disks in Parthenocissus tricuspidata during Attachment (착생에 따른 담쟁이덩굴 흡착근의 부착 특성)

  • Lee, Myung-Hui;Kim, In-Sun
    • Applied Microscopy
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    • v.41 no.2
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    • pp.139-145
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    • 2011
  • Parthenocissus tricuspidata is an epiphyte that lacks a main axial stem, but develops adhesive disks along the stem for climbing support. In this study, scanning electron microscopy (SEM) and energy dispersive X-ray spectroscopy (EDS) were utilized to examine the brick wall surface and the adhesive disks of P. tricuspidata that attached to the surface successfully. The study was mainly focused the outermost layers of both structures before and after adhesion to find out whether there has been some structural and/or physical interactions between the two. The adhesive disks adhered firmly to the brick wall by secreting adhesive materials that help them for a tight attachment to the surface. The rough wall surface appeared facilitating better attachment of the adhesive disks by infiltrating the materials into those spaces leading to some degree of interactions at the interface. EDS analysis on the outermost layers of the adhesive disks that were separated from the substrates was also consistent with the SEM data on the interaction between the adhesive disks and the substrate surface. EDS analysis of the brick wall surface and the adhesive disks demonstrated similar elements of O, Si, Fe, Al, K, Mg, and Na in their components.