• Journal of Life Science
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• v.22 no.9
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• pp.1173-1179
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• 2012

The importance of genetic stability and bio-safety in the environment has recently been recognized for many (genetically modified) GM plants. This study evaluated the GM safety of transgenic rice and its environmental variance. Data on agronomic characters and principal component were collected for vitamin A-enriched GM rice and four check cultivars in a large GM field trial during 2009-2011. The cultivation environment was a large GM field and a greenhouse. In this experiment, there was no significant difference between the agronomic characters of the GM rice and those of a donor plant, 'Nagdong'. In terms of grain characteristics, the appearance and physicochemical characteristics of the GM rice and those of the donor plant were similar. However, the grain of the GM rice developed a white core and a white belly when planted in the greenhouse. The type and distribution of dominant weed species were not different in the GM rice and the 'Nagdong'. In addition, gene flow was not detected in the dominant weed species based on PCR analysis.

• Journal of Life Science
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• v.21 no.12
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• pp.1726-1731
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• 2011

Human Stem Cell Factor (hSCF) is a cytokine that binds to the c-Kit receptor and plays an important role in hematopoiesis, spermatogenesis, and melanogenesis. To produce the human Stem Cell Factor (hSCF) recombinant protein, we constructed a germline transgenic silkworm using the piggyback vector. The expression of the hSCF gene was driven by the Drosophila heat shock protein 70 (dHsp70) promoter. 3XP3 promotor-driven EGFP was used as a marker which allowed us to rapidly distinguish the transgenic silkworm. A mixture of the donor and helper vector was micro-injected into 1,020 eggs of bivoltin silkworms, Keomokjam. We obtained approximately 22 G1 broods that were EGFP-positive. The expression of the hSCF gene in the transgenic silkworm was analyzed by SDS-PAGE and immunoblotting. Also, analysis of insertion sites into the silkworm genome using inverse PCR showed that exogenous DNA was inserted into the transgenic silkworm genome. These results show that successfully constructed transgenic silkworm expresses the hSCF recombinant protein.

• Journal of Life Science
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• v.27 no.12
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• pp.1494-1499
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• 2017

Oral infection due to Candida albicans is a widely recognized and frequent cause of superficial infections of the oral mucosa (oral candidiasis). Although oral candidiasis is not a life-threatening fungemia, it can cause severe problems in individuals under certain conditions. MicroRNAs (miRNAs) are noncoding, small RNA molecules, which regulate the expression of other genes by inhibiting the translation of target mRNAs. The present study was designed to identify miRNAs in C. albicans and determine their possible roles in this organism. miRNA-sized small RNAs (msRNAs) were cloned in C. albicans by deep sequencing, and their secondary structures were analyzed. All the cloned msRNAs satisfied conditions required to qualify them as miRNAs. Bioinformatics analysis revealed that two of the most highly expressed C. albicans msRNAs, Ca-363 and Ca-2019, were located in the 3' untranslated region of the corticosteroid-binding protein 1 (CBP1) gene in a reverse orientation. miRNA mimics were transformed into C. albicans to investigate their RNA-inhibitory functions. RNA oligonucleotide-transformed C. albicans was then observed by fluorescent microscopy. Quantitative PCR analysis showed that these msRNAs did not inhibit CBP1 gene expression 4 hr and 8 hr after ectopic miRNA transformation. These results suggest that msRNAs in C. albicans possess an miRNA-triggered RNA interference gene-silencing function, which is distinct from that exhibited by other eukaryotic systems.

• Korean Journal of Microbiology
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• v.52 no.2
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• pp.175-182
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• 2016

In this study, we isolated aromatic compounds (lignin polymers) utilizing bacteria in humus layer of oak forest and investigated phylogenetic characteristics and correlation with major bacterial populations in the humus layer by pyrosequencing. Forty-two isolates using aromatic compounds such as p-anisic acid, benzoic acid, ferulic acid and p-coumaric acid were isolated and phylogentic analyses based on 16S rRNA gene sequences showed that the isolates belonged to the genus Rhizobium, Sphingomonas, Burkhorlderia, and Pseudomonas. Among these, Burkhorlderia species which belong to Betaproteobacteria class occupied 83% among the isolates. The bacterial populations in humus layer of oak forest were characterized by next generation pyrosequencing based on 16S rRNA gene sequences. The humus sample produced 7,862 reads, 1,821 OTUs and 6.76 variability index with 97% of significance level, respectively. Bacterial populations consist of 22 phyla and Betaproteobacteria were the major phylum consisting of 15 genera including Burkholderia, Polaromonas, Ralstoria, Zoogloea, and Variovorax. Approximately fifty percentage of them was Burkholderia. Burkholderia as the majority of population in the humus was considered to play a role in degrading lignin in humus layer of oak forest.

• Journal of the Korean Geographical Society
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• v.50 no.6
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• pp.659-675
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• 2015

This study explores the status, characteristics and problems of urban-to-rural migration policy in Gyeongbuk Province, and suggests some improvements based on this analysis. Gyeongbuk Province enacted local ordinances related to urban-to-rural migration for the first time in Korea, and has expanded the area of its own projects in addition to the central government's support projects. Consequently, the degree of satisfaction for the support projects in Gyeongbuk Province is higher than in other provinces. Problems of the support projects for urban-to-rural migration are the lack of role sharing between central and lower level local government, and the lack of connectivity among the relevant departments; the non-reflection of regional characteristics and attributes of urban-to-rural migrants (household); and the insufficient satisfaction of policy demands by non-agricultural urban-to-rural migrants. Improvements for these problems include establishing governance that involves urban-to-rural migrants in addition to the existing policy actors, and institutionalizing the project to properly embed this governance in the region. In addition to economic and physical support, diverse programs based on the adaptive cycle, 'non-agricultural rural jobs' for nonagricultural urban-to-rural migrants, and support programs for professional competency enhancement contributing to rural communities should be developed.

• The Journal of Korean Institute of Communications and Information Sciences
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• v.35 no.8B
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• pp.1129-1140
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• 2010

Wireless mesh networks have been widely studied as the next generation technology to solve the problems of conventional wireless networks. Particularly, Mobile WiMAX based wireless mesh networks are noticed due to many advantages. Mobile WiMAX standard provides two kinds of layer-3 handover schemes: Mobile IP and Proxy Mobile IP based handover schemes. MIP based handover scheme has a problem in that it incurs the long handover latency because mobile nodes generate a lot of handover messages. On the other hand, PMIP based handover scheme decreases the handover latency by reducing the number of handover messages, because mobile nodes do not participate in handover procedure. Therefore, layer-3 handover for Mobile WiMAX should be designed based on PMIP. However, conventional PMIP based handover still has latency overhead, because of many message exchanges between PBU and PBA after completing the layer-2 handover. Hence, in this paper, we propose a fast layer-3 handover scheme that achieves the lower handover latency for Mobile WiMAX based wireless mesh networks. Proposed scheme has advantages in terms of handover latency. Simulation results show that proposed scheme achieves low handover latency during the layer-3 handover.

• The Journal of the Korea institute of electronic communication sciences
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• v.13 no.4
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• pp.765-776
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• 2018

Artificial Intelligence is a rapidly growing technology with the latest developments in computing technology and is considered as one of the next major technologies. Chat-Bot is a system that is designed to respond to user's input according to the rules that are set up in advance and it provides more services through simple and repetitive tasks such as counseling, ordering and others. Accordingly, the study aims to present a model of a celeb-bot using Artificial Intelligence. Celeb-Bot is a combination of Celeb, which are short for Celebrity and Chat-bot. Celeb-Bot provides a Chat-Bot service that allows people to talk to a celebrity. The celeb is the best thing to build a relationship and has the advantages of being accessible to anyone. At the same time, Artificial Intelligence is a technology that can be seen as a person, not a product. Based on this, we believe that Celeb's Characteristic and Chat-bot based on artificial intelligence technologies need to be combined, so variety of products can generate synergy. It is predicted that there will be variety of derivatives that utilize this technology, and it is going to present a celeb-bot model accordingly.

• Journal of the Korean Institute of Traditional Landscape Architecture
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• v.38 no.2
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• pp.118-124
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• 2020

The purpose of this study was to explore the ecological aesthetic features and the implications for ecological landscape design by analyzing the design philosophy and ecological artworks of Patricia Johanson, an ecological artist. Literature review and a field visit were conducted to investigate Johanson's four art projects that express the functional purpose, artistry, and locality of symbiosis with nature and restoration of ecosystem habitats. As the results of analysis, first, Johanson's work created 'functional art work' and 'artistic infrastructure'. Second, the historical and regional characteristics were expressed artistically and symbolically. Third, Johanson is characterized by connecting humans and nature through the publicity of works of art, and raising the awareness of preservation of nature and environment among the community and children. Johanson's innovative works can provide inspiration for landscape architects pursuing functional and sustainable landscape design.

• The KIPS Transactions:PartD
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• v.18D no.3
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• pp.157-168
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• 2011

Recently flash memory has been being utilized as a main storage device in mobile devices, and flashSSDs are getting popularity as a major storage device in laptop and desktop computers, and even in enterprise-level server machines. Unlike HDDs, on flash memory, the overwrite operation is not able to be performed unless it is preceded by the erase operation to the same block. To address this, FTL(Flash memory Translation Layer) is employed on flash memory. Even though the modified data block is overwritten to the same logical address, FTL writes the updated data block to the different physical address from the previous one, mapping the logical address to the new physical address. This enables flash memory to avoid the high block-erase cost. A flashSSD has an array of NAND flash memory packages so it can access one or more flash memory packages in parallel at once. To take advantage of the internal parallelism of flashSSDs, it is beneficial for DBMSs to request I/O operations on sequential logical addresses. However, the B-tree structure, which is a representative index scheme of current relational DBMSs, produces excessive I/O operations in random order when its node structures are updated. Therefore, the original b-tree is not favorable to SSD. In this paper, we propose AS(Always Sequential) B-tree that writes the updated node contiguously to the previously written node in the logical address for every update operation. In the experiments, AS B-tree enhanced 21% of B-tree's insertion performance.

• KIPS Transactions on Computer and Communication Systems
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• v.2 no.2
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• pp.67-74
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• 2013

Approximate string matching problems have been studied in diverse fields. Recently, fast approximate string matching algorithms are being used to reduce the time and costs for the next generation sequencing. To measure the amounts of errors between two strings, we use a distance function such as the edit distance. Given two strings X(|X| = m) and Y(|Y| = n) over an alphabet ${\Sigma}$, the edit distance between X and Y is the minimum number of edit operations to convert X into Y. The edit distance between X and Y can be computed using the well-known dynamic programming technique in O(mn) time and space. The edit distance also can be computed using the Four-Russians' algorithm whose preprocessing step runs in $O((3{\mid}{\Sigma}{\mid})^{2t}t^2)$ time and $O((3{\mid}{\Sigma}{\mid})^{2t}t)$ space and the computation step runs in O(mn/t) time and O(mn) space where t represents the size of the block. In this paper, we present a parallelized version of the computation step of the Four-Russians' algorithm. Our algorithm computes the edit distance between X and Y in O(m+n) time using m/t threads. Then we implemented both the sequential version and our parallelized version of the Four-Russians' algorithm using CUDA to compare the execution times. When t = 1 and t = 2, our algorithm runs about 10 times and 3 times faster than the sequential algorithm, respectively.