• The Journal of the Korea Contents Association
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• v.5 no.1
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• pp.37-44
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• 2005

In this study, we integrated 40 kinds of aerobic bacteria that has a higher appearance rate and data for experts and educations, and constructed the aerobic bacterial images database. Constructed system is useful to culture of bacteria for novice without expert's heuristics and deep knowledge and to decrease time and money for handling of patient's examination results. Moreover, it can add new bacterial information in database and contribute to raise a medical quality and it is useful to support a expert's technology in department of laboratory medicine.

• Korean Journal of Microbiology
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• v.54 no.3
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• pp.175-187
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• 2018

Myxobacteria produce diverse secondary metabolites for predation, self-defense, intercellular signaling, and other unknown functions. Many secondary metabolites isolated from myxobacteria show pharmaceutically useful bioactivity such as anticancer, antibacterial, and antifungal activities with a unique mechanism of action. Therefore, a large number of myxobacterial strains have been isolated globally and many bioactive compounds have been purified from them. However, 16S rRNA database analysis indicates that there are far more types of myxobacterial species in the wild than have ever been isolated, and genome sequence analysis suggests that each myxobacterium is capable of producing much more metabolites than already known. In this article, the current status of studies on the secondary metabolites from myxobacteria, their biosynthetic genes, biological functions, and transcriptional regulatory factors governing gene expression were reviewed.

• Journal of the Korean Geographical Society
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• v.34 no.5
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• pp.473-491
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• 1999

세균성 이질은 국내 제1종 법정 전염병으로 분류되어 관리되고 있는 질환으로서 1998년 이후 그 발병 사례가 급속히 증가하고 있다. 본 연구는 1999년 3월 부산시 사상구에서 집단 발병한 세균성 이질을 대상으로 하여, 각 환자들의 발병 시점과 장소의 분포패턴에 대한 지리학적 고찰을 목적으로 한다. 환자분포의 특징적 공간패턴과 그들의 시계열적 확산 양상 등을 탐색하기 위한 방법론은 보건지리학과 지도학 및 공간통계학에 기반을 둔 공간분석기법을 중심으로 설정하였다. 분석자료는 해당 지역의 수치지형도, 지적도, 인구 센서스 자료를 포함한 GIS 데이터베이스로 구축되었다. 인구분포를 감안한 밀도구분도를 바탕으로 개별환자의 위치자료와 동 단위로 집계된 자료를 자료의 형태에 따라 분석기법을 달리하였으며, 환자 발생 밀도, 상대적 위험지수 등을 지도화하여 역학자료의 시각적 통계적 분석을 수행하였다. 환자분포의 공간적 중심위치와 분산의 변화 등 기술적 통계분석과 함께 제1차 공간속성을 커널추정법으로 찾아보았다. 이와 더불어 ‘공간적 의존성’과 관련된 제2차 공간속성은 K-함수와 시뮬레이션을 통해 분석하여 군집성 등이 통계적으로 확인되었다. 본 연구를 통해 역학조사시 GIS의 활용사례가 제시되었으며, 모집단 인구를 고려한 확률지도 작성 기법과 다양한 데이터 가시화 방법, 그리고 시계열별 발생 환자들의 지리적 변이를 분석 하는데 따르는 문제들이 논의되었다.

• Journal of the Korea Society of Computer and Information
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• v.10 no.1 s.33
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• pp.93-100
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• 2005

This study is to desist and implement an efficient microbial medical image retrieval system based on knowledge and content of them which can make use of more accurate decision on colony as doll as efficient education for new techicians. For this. re first address overall inference to set up flexible search path using rule-base in order U redure time required original microbial identification by searching the fastest path of microbial identification phase based on heuristics knowledge. Next, we propose a color ffature gfraction mtU, which is able to extract color feature vectors of visual contents from a inn microbial image based on especially bacteria image using HSV color model. In addition, for better retrieval performance based on large microbial databases, we present an integrated indexing technique that combines with B+-tree for indexing simple attributes, inverted file structure for text medical keywords list, and scan-based filtering method for high dimensional color feature vectors. Finally. the implemented system shows the possibility to manage and retrieve the complex microbial images using knowledge and visual contents itself effectively. We expect to decrease rapidly Loaming time for elementary technicians by tell organizing knowledge of clinical fields through proposed system.

• Food Industry And Nutrition
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• v.10 no.1
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• pp.11-16
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• 2005

식물성 단백질의 주요 공급원이며 우리나라 전통식품 중의 하나인 두부의 유통기한을 정량적으로 예측할 수 있는 수학적 모델을 개발하고자 온도와 초기균수에 따른 두부 부패세균의 성장 실험 결과를 데이터베이스화하여 이를 바탕으로 균의 성장을 정량적으로 평가할 수 있는 수학적 모델을 개발하였다. 근의 증식 지표인 최대증식속도상수(k), 유도기(LT), 세대시간(GT)은 온도에 지배적인 영향을 받았으며, 초기균수에 따른 유의 적 인 차이 는 없었다(p<0.05). 최대증식속도상수와 온도 및 초기균수의 상관관계를 나타내는 수학적 정량평가모델인 square root model을 이 용하여 두부 부패 세균의 성장을 정량적으로 예측할 수 있는 모델$({\surd}{\kappa}=0.016861(T+6.87095))$을 개발하였으며 실험치와 예측치의 상관계수는0.969이었다. 이 예측 정량평가모델로부터 예측한 최대증식속도상수와 두부의 관능적 부패시 점을 반영 한 Gompertz 변형 모델을 이용하여 두부의 유통기한을 예측할 수 있는 모델$(Spoilage-critrion(hr)=\frac{2{\times}Ln2+Ln[(Nmax/No)-1])}{k}$을 개발하였다

• Archives of design research
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• v.18 no.1 s.59
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• pp.17-26
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• 2005

Nowadays, display-contents are developing to an informative environment. that is under the logic of the media operating system. To perceive the media-environments and produce the cultural contents, the cultural designers seek to understand a skin structure from making up for shape. To appreciate operating system in data and database is not only systematization of form and contents of visual contents but also variety contents into multiple-platform and integrative environments. These days, the spectacle exhibition try to express for their surface design between algorithm of data and database. the information is expressing aesthetic which means presents the integrated contents through the play instinct environment to end-user. That was given web or game to participation is developing with the cellular device and ubiquitous computing system. in the linear perspective, the end-user should be immerse more and more hyper-simulation system because of the operating algorithm of database. To do this, human have need to get the information-ability from multi-platform society. In the virtual environment, database offer the experience of an unheard-of event to end-user that prepare the participants the circumstances priority of signifiers. To do that already based on a fixed sensibility endow with narrative of the freshness- experience.

• Journal of Digital Convergence
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• v.14 no.10
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• pp.349-354
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• 2016

The emergence and dissemination of extended-spectrum ${\beta}$-lactamse (ESBL) producing Klebsiella pneumoniae isolates make it more difficult to treatment of bacterial infections. In our study, we detected ESBL genes and investigated antimicrobial susceptibility of K. pneumoniae isolates in Chungcheong province. In addition, clonality among the isolates was analyzed by repetitive element sequence (REP)-PCR. Twenty-one of 102 K. pneumoniae isolates produced CTX-M-14 and/or CTX-M-15 and showed high level (over 70%) resistance to third cephalosporins. CTX-M type ESBL producing K. pneumoniae strains isolated in our study showed diverse clonality and some of the isolates have been disseminated in the community. Enhancing infection control will be needed to prevent dissemination of the K. pneumoniae isolates. In addition, for more effective control of resistant bacteria it is considered necessary to monitor the database constructed through convergence of biological investigation and statistical analysis of antimicrobial resistance genes.

• Journal of Life Science
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• v.18 no.11
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• pp.1617-1624
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• 2008

Chitin and chitosan, which is deacetylated form of chitin, are one of the most abundant biomass on the earth. They showed various biological activities including antimicrobial activity, heavy metal chelating, immune system activation, and have very diverse applications in food, pharmaceutical, medicinal, and environmental industry. There have been reported many chitin/chitosan-hydrolyzing enzymes, their structures and genes from three domains, archaea, bacteria, and eukarya. Carbohydrate hydrolyzing enzymes are classified in CAZy (Carbohydrate Active Enzymes) database according to their amino acid sequence similarity. Interestingly, chitinases and chitosanases are classified in various glycosyl hydrolase(GH) families, GH2, GH5, GH7, GH8, GH18, GH19, GH20, GH46, GH48, GH73, GH75, GH80, GH84, and GH85. Here, we review characteristics and structures of chitin/chitosan hydrolyzing enzymes according to glycosyl hydrolase families in order to provide information about gene mining.

• Korean Journal of Clinical Laboratory Science
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• v.55 no.4
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• pp.244-252
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• 2023

The timeliness and accuracy of test results are crucial factors for clinicians to decide and promptly administer effective and targeted antimicrobial therapy, especially in life-threatening infections or when vital organs and functions, such as sight, are at risk. Further research is needed to refine and optimize matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS)-based assays to obtain accurate and reliable results in the shortest time possible. MALDI-TOF MS-based bacterial identification focuses primarily on techniques for isolating and purifying pathogens from clinical samples, the expansion of spectral libraries, and the upgrading of software. As technology advances, many MALDI-based microbial identification databases and systems have been licensed and put into clinical use. Nevertheless, it is still necessary to develop MALDI-TOF MS-based antimicrobial-resistance analysis for comprehensive clinical microbiology characterization. The important applications of MALDI-TOF MS in clinical research include specific application categories, common analytes, main methods, limitations, and solutions. In order to utilize clinical microbiology laboratories, it is essential to secure expertise through education and training of clinical laboratory scientists, and database construction and experience must be maximized. In the future, MALDI-TOF mass spectrometry is expected to be applied in various fields through the use of more powerful databases.

• Journal of Mushroom
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• v.22 no.1
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• pp.22-26
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• 2024

Tricholoma matsutake is a traditional favorite food in East Asia, cultivated in fairy rings called "shiro," which are found near Pinus densiflora. For effective artificial cultivation of Tri. matsutake, microorganisms from symbiotic fairy rings are co-cultivated. In this study, one bacterial isolate (Y22_B35) and two fungal isolates (Y22_F64 and Y22_F68) displayed growth-promoting effects on Tri. matsutake mycelium (158.47, 125.00, and 122.26% enhanced growth, respectively). For identification, 16S rRNA or ITS regions from the microorganisms¡¯ genomes were sequenced. Other sequences, including BenA, CaM, and RPB2 were sequenced in the fungal isolates. The bacterial isolate Y22_B35 was identified as Bacillus cereus. Y22_F64 and Y22_F68 were identified as Umbelopsis nana and Aspergillus parvulus, respectively. To identify the effects of the dominant microorganisms on Tri. Matsutake cultivation, metagenomic analyses were performed. Discovery of these Tri. matsutake mycelium growth-promoting microorganisms and metagenomics analyses are expected to contribute to our understanding of Tri. matsutake fruiting body growth and construction of biomimicry.