• Korean Journal of Fisheries and Aquatic Sciences
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• v.35 no.5
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• pp.534-541
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• 2002

The use of triorganotins as insecticides, bacteriocides, and antifouling agents has increased dramatically over the past 30 years, In this paper, to evaluate the acute and chronic toxicity effects of various triorganotins (TBTO, TBTE, CTPT, TBTCI) on the growth of microalgae, Skeletonema costatum, we carried out the short term and long term experiments, respectively. In the short-term experiments, the growth of S. costatum was inhibited by the various triorganotins in the dose dependent manners, but recovered by the time laps. The growth inhibition of triorganotins were decreased in the groups with higher initial cell density. These data were resulted from the dilution effect and the biodegradation of triorganotins by the microalgae. In the long-term experiments, S. costatum died completely at the 1 $\mu$g/L of TBTP and CTPT, but didn't in short-term experiments. The facts suggest that the toxicity of the chronic exposure of triorganotins should be increased in the natural sea water with low phytoplankton density.

• Journal of the Korean Society of Food Science and Nutrition
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• v.32 no.6
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• pp.931-936
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• 2003

We investigated inhibitory effects of Hericium erinaceus on the growth of cancer cells and the expression of cell cycle regulators, cyclins. Anticancer effects of Hericium erinaceus extract and fractions against cancer cell lines including HepG2 and HT29 were investigated. The methanol extract, the hexane fraction, the chloroform fraction and the ethylacetate fraction of Hericiu erinacew inhibited growth of cancer cells but they had no effect on the cytotoxicity of normal human liver cells under the same conditions. As shown by western blot analysis, the expression of cyclin B1 known as cell cycle regulator was markedly decreased after treatment with Hericium erinaceus extract in HepG2 cells. These results suggest that antiproliferative effect of Hericaum erinaceus extract is associated with markedly decreased expression of cyclin B1.

• Journal of the Korean Society of Food Science and Nutrition
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• v.31 no.2
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• pp.329-332
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• 2002

We investigated the effect of Sarcodon aspratus extract on expression of cell cycle regulators. Methanol extract of Sarcodon aspratus showed a growth suppression on HepG2. As shown by western blot analysis, the expressions of cyclin A and Dl known as cell cycle regulators were decreased after treatment of Sarcodon aspratus extract. On the other hand, the expression of cyclin Bl was increased in the presence of Sarcodon aspratus extract. Furthermore, the expression of p53, a tumor supressor gene, and p27, a cell cycle dependent protein kinase inhibitor, were increased, whereas the expression of PCNA was decreased. In conclusion, our study suggests that growth inhibitory effect of Sardodon aspratus methanol extract on HepG2 is induced by cell cycle arrest in the Gl phase caused by decrease in cyclin A, Dl expressions and increases in p53, p27 expression.

• Proceedings of the Korea Society of Environmental Toocicology Conference
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• 2001.05a
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• pp.118-118
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• 2001

Lemna gibba에 대한 성장저해시험은 OECD Lemna Growth Inhibition Test(1998)와 EPA Auqatic Toxicity Test Using Lemna sp, (OPPTS 850.4400)에 제시된 시험 방법으로, 시험물질에 대한 수중식물의 독성값을 알기 위하여 광범위하게 사용되고 있다. 본 실험은 각각의 Guideline에서 제시된 endpoint를 서로 비교하여, 가장 효과적으로 시험의 결과를 표현 할 수 있는 endpoint를 찾고자 하였으며, 이때 사용된 endpoint로는 잎의 수, 잎 면적, 습중량, 건중량을 이용하였다. (중략)

• Journal of the Korean Society of Food Science and Nutrition
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• v.34 no.3
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• pp.317-322
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• 2005

This study was carried to investigate the inhibition effects of Lepista nuda mushroom extracts on the growth of the human cancer (HepG2, KATOⅢ, AGS) cells. The fraction Ⅰ of Lepista nuda mushroom extracts strongly inhibited to 71.4-91.8% for the growth of cancer cells. The growth of cancer HepG2, KATOⅢ and AGS cell which treated with 0.5 and 1㎎/mL of hot water fraction I was inhibited to 71.4% and 85.5%, 45.0% and 86.6%, 71.6% and 90.7% respectively. The growth of cancer cell HepG2, KATOⅢ and AGS which treated with 0.5 and 1㎎/mL of microwave fraction I was inhibited to 79.8% and 85.2%, 78.6% and 88.9%, 85.8% and 91.8%, respectively. The inhibition ratios of cancer cell growth were higher by microwave extracts than hot water extracts. And the more fraction were insoluble for water, the more inhibition ratios of cancer cell growth were lower.

• Korean Journal of Food Science and Technology
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• v.23 no.3
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• pp.370-374
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• 1991

The inhibitory effects of garlic on the mutagenicity in Salmonella assay system and on the growth of HT-29 human colon carcinoma cells were studied. Methanol extract of garlic inhibited the mutagenicities induced by aflatoxin $B_1(AFB_1)$and N-methyl-N'-nitro-N-nitrosoguanidine(MNNG) in Salmonella typhimurium TA100. The inhibition rate increased significantly when the concentration of the methanol extract from garlic increased in both strains of Salmonella typhimurium TA98 and TA100. The chloroform fraction from the methanol extract exhibited strong antimutagenicity against $AFB_1$. The chloroform fraction also inhibited greatly the growth of human HT-29 colon carcinoma cells in fetal bovine serum concentrations of 1% and 5%.

• Journal of the Korean Society of Food Science and Nutrition
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• v.30 no.5
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• pp.806-813
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• 2001

This study was carried out to investigate the effects of various kinds of commercial salts including Chunil, Hanju, Guwoon and Bamboo salts on the growth of microorganisms involved in kimchi fermentation. Among various microorganisms related to the kimchi fermentation, the growth of Leuconostoc mesenteroides, Lactobacillus plantarum, Pichia membranaefaciens and E. coli was examined. Based on the conditions of kimchi fermentation, 3% and 5% concentration of each salt were studied. Also, the temperatures at 1$0^{\circ}C$, 18$^{\circ}C$ and 37$^{\circ}C$ of the cultural condition were examined. The growth of Leuconostoc mesenteroides was inhibited depending on the reduction of cultural temperature and increase of concentration of salts. There was no considerable difference on the growth of Leu. mesenteroides in the different the kind of salts. However, the growth of Lactobacillus plantarum was strongly inhibited by the 5% concentration of Bamboo salt during incubation at 18$^{\circ}C$. When Lactobacillus plantarum was cultured at 1$0^{\circ}C$, its growth was remarkably decreased regardless of the kind and concentration of salts. In the case of Pichia membranaefaciens, Bamboo salt strongly inhibited its growth at all cultural temperatures. Apparent inhibitory effect on the growth of E. coli was observed by the Bamboo salt treatment during the incubation at 18$^{\circ}C$. At the cultural temperature of 1$0^{\circ}C$, similar results obtained.

• Journal of Life Science
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• v.17 no.12
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• pp.1723-1728
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• 2007

Rubus coreanus Miquel (RCM), a type of red raspberry, grows wild in Korea and China and its unripe fruit is used as a folk medicine for the treatment of impotence and as a diuretic. RCM was extracted with methanol and then further fractionated it into for different types. In this study, we investigated the antioxidant activity of a RCM extract (ext.) and its fraction (fr.). DPPH free radical scavenging activity assay, total polyphenols contents, total flavonoids contents assay were used to analyze antioxidant activity. The DPPH free radical scavenging activity $(RC_{50}:1.67{\mu}g/ml)$ and total polyphenols contents $(546.25{\mu}g/mg)$ were higher in butanol fraction than in other fr. And total flavonoids contents was higher in ethylacetate fr. $(141.78{\mu}g/mg)$. We applied comet assay to measure the DNA damage in the individual cells and exposed time course at $IC_{50}$. Comet assay is a rapid and sensitive fluorescent microscopic method to examine DNA damage and repair at individual cell level. The butanol fro from RCM significantly induced 54.12%, 57.95% of DNA damage after treated RCM for 8 hr. In conclus

• Korean Journal Plant Pathology
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• v.12 no.2
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• pp.245-250
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• 1996

식물병원균 Botrytis cinerea와 Fusarium oxysporum에 대한 생물학적 방제의 기초자료를 얻기 위하여 살충성 진균 Beauveria bassiana의 식물병원균에 대한 균사성장저해, 포자발아 억제, 균사와 포자의 형태변화 등의 효과를 살펴보았다. 평판배지 상에서 두 식물병원균의 균사생장이 저해되었으며, 저해효과는 배지종류에 따라 달랐는데 B. cinerea의 경우 PDA배지에서 가장 크게 저해되었으며 F. oxysporum의 경우에는 TSA배지에서 가장 크게 저해되었다. B. bassiana는 $25^{\circ}C$에서 6일간 배양했을 때 F. oxysporum에 대한 항균력이 가장 높았으며 동시에 최대의 균체량을 생산하였다. 또한 B. bassiana의 배양여액을 식물병원균에 30% 농도로 첨가하여 배양했을 때 식물병원균의 포자 발아율은 B. cinerea, F. oxysporum에서 각각 30%(control: 88.2%), 10.0%(control: 78.6%)로 낮아졌으며 발아 개시 시간도 4~8시간 지연되었다. 현미경을 통한 미세구조관찰에서는 10%의 B. bassiana배양여액을 첨가했을 때 F. oxysporum의 포자 크기가 1/2~1/3으로 줄어들었으며 균사와 격막의 형태도 비정상적으로 변하여 균사외막과 격막-격막 사이의 구분이 불명확해졌다.

• Journal of dental hygiene science
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• v.7 no.1
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• pp.9-12
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• 2007

Streptococcus mutans is the major causative factor in dental caries and has been known to induce dental caries by the process of initial attachment proliferation and acid production. The pH of Streptococcus mutans(S. mutans) media and glucosyltransferase(GTase) activity were determined to evaluate the anticariogenic activity of Dioscorea batatas and Prunella vulgaris hexane fraction. In the experiment of hexane fraction of Dioscorea batatas, the relative growth ratio(RGR) against Streptococcus mutans (S. mutans) was determined as 93% in concentration of 0.125 mg/ml, 100% in 0.25 mg/ml. The hexane fraction of Prunella vulgaris revealed relative growth ratio(RGR) against Streptococcus mutans (S. mutans) as 86% in concentration of 0.25 mg/ml, 100% in 0.5 mg/ml. The relative growth ratio(RGR) against of hexane fraction were determined as 92% in concentration of 0.25 mg/ml, 100% in 0.5 mg/ml. The acidities were pH 7.2 on Dioscorea batatas and 7.0 on Prunella vulgaris in 2.0 mg/ml in contrast to pH 5.6 on Dioscorea batatas and 5.2 on Prunella vulgaris in control. The inhibitory effect to activity revealed 35% on Dioscorea batatas and 25% on Prunella vulgaris in 2.0 mg/ml. The inhibitory effect of Dioscorea batatas was more potent than Prunella vulgaris.