• 한국임상수의학회지
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• 제27권4호
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• pp.445-449
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• 2010

10세령의 암컷 말티즈가 족부 괴양 및 안면부 괴양성 피부병으로 서울대학교 수의과대학 동물병원에 내원하였다. 환자는 장기간의 페노바르비탈 투여 경력을 가지고 있었으며, 안면부 및 족부의 피부병변의 분포 양상과 간효소 수치의 상승 및 초음파 영상에서의 간의 저에코성병변들을 참고하여, 최종적으로 피부 생검을 통해 표재성 괴사성 피부염으로 진단하였다. 8개월간의 간보조제 경구 투여 및 아미노산제제의 말초 혈액 투여로 피부 증상은 약간 호전되었으나, 비가역적인 간의 손상은 회복이 힘들었으며, 8개월간의 치료 후 사망하였다.

• 한국초지조사료학회지
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• 제22권3호
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• pp.209-212
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• 2002

이탈리안 라이그라스(Lolium multiflorum Lam.) 계통 A의 약(葯) 배양을 실시하여 약(葯) 유래의 식물체를 생산하였다. MS 기본배지에 sucrose 30 g/$\ell$, NAA 2 30 mg/$\ell$, kinetin 1 30 mg/$\ell$을 첨가한 캘러스 유도배지에서 배양 20일만에 약(葯) 유래의 캘러스를 유도하였다. 캘러스 유도율은 9.2%였으며, 캘러스의 평균 크기는 8.6mg/callus/anther 였다. MS 기본배지에 sucrose 30 g/$\ell$, NAA 1 mg/$\ell$, kinetin 2 mg/$\ell$을 첨가한 재분화 배지에 캘러스를 치상하여 배양 50일에 완전히 재분화된 식물체를 얻었으며, 캘러스로부터 식물체로 재분화되는 비율은 26% 였다.

• 한국초지조사료학회지
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• 제20권1호
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• pp.1-6
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• 2000

Improvement of cold-tolerance of Italian ryegrass(Lo1ium multiflorum L.) is an important breeding objectivefor enlargement of cultivation area in Korea. To develop the cold-tolerant variety of Italian ryegrass,cold-tolerant clones survived under - 13- - 14$^{\circ}$C of minimum average air temperature in January wereselected in the area of Dun Nae, Kwangwon Province. The 5 lines of Italian ryegrass clones selected werepolycrossed for seed production. Synthetic seeds were examined on growth characters and forage productionsin Suwon from 1996 to 1998, in Unbong 1997 and in Younchun 1998, respectively. The growth charactersand forage productions of Italian ryegrass variety named as "Hwasan 101" were summarized as follow ;ltalian ryegrass variety, Hwasan 101 was tetraploid and showed semi-prostrate growth habit in autumn andwas not only dark green in leaf color and broad in flag leaf width but also excellent in leafiness. Also, itshowed low plant height at first heading date of 20th May and excellent lodging tolerance. Expecially,Hwasan 101 in all trial regions was 80 -90% of winter survivals that was higher than 60 -90% of Florida80 and Marshall varieties. Therefore we estimate that Hwasan 101 can be cultivated in regions that is higherthan -9$^{\circ}$C in minimum average air temperature and lower than 400m in sea level. In forage production ofHwasan 101, fresh weight and total digestible nutrient(TDN) yield were 66,940 and 6,551kghq and drymatter yield was 10,050kgha. In conclusion, Italian ryegrass, Hwasan 101 was medium and late maturingvariety but excellent in cold-tolerance, lodging-tolerance, leafiness, and forage production.(Key words : Italian ryegrass, Cold tolerance, New variety)ian ryegrass, Cold tolerance, New variety)

• 한국초지조사료학회지
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• 제21권1호
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• pp.31-34
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• 2001

이탈리안 라이그라스 (Lolium multiflorum Lam.) 의 절단한 줄기를 살균처리한 다음, IBA론 첨가한 농도별로 5종의 배지에 처리별로 각각 20개씩 절단한 줄기를 꽃은 후, 4$^{\circ}C$ 암상태에서 40일간 보관하였다가. 실온에 하루동안 둔 다음, 26$^{\circ}C$로 유지되는 생장실에서 배양하며 뿌리가 유도되는 정도를 관찰하였다. 생장실에서 배양한 지 1개월째에 뿌리유도 정도를 조사한 결과, MS-0 처리구에서 0.0%, MS-0.5IBA 처리구에서 8.3%, MS-1.0IBA 처리구에서 37.5%, MS-1.5IBA 처리구에서 16.7%, MS-2.0IBA 처리구에서 12.5%의 뿌리 유도를 확인하였다. MS 배지에 1.0 mg/$\ell$의 IBA를 첨가한 처리구에서 뿌리 유도 정도가 37.5%로서 가장 좋았다. 따라서 이탈리안 라이그라스 줄기로부터 뿌리를 유도할 경우에는 IBA를 첨가해 주는 것이 뿌리 유도 효율을 높일 수 있는 것으로 판단되며, 1.0mg/$\ell$의 IBA를 첨가해 줄 때 뿌리 유도 효율이 가장 좋을 것으로 사료된다.

• 한국초지조사료학회지
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• 제21권1호
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• pp.35-38
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• 2001

버즈풋 트레포일 (Lotus corniculatus L.)의 줄기부분을 절단하여, SH 배지에 IBA를 첨가한 농도별로 뿌리 유도 정도를 조사한 결과. IBA 농도가 높을수록 뿌리 유도가 좋았으며, 2.5 mg/$\ell$의 IBA를 첨가한 처리구에서 뿌리 유도 정도가 90~95%로서 가장 좋았다. 캘러스로부터 shoot를 유도한 다음 줄기를 취하여 처리한 경우에는, 처리 12일째에 1/2 SH-0 배지에서 9개 (45%), SH-0 배지에서 10 (50%), SH-0.5IBA 배지에서 10개 (50%), SH-1.0IBA 배지에서 10개 (50%), SH-1.5IBA 배지에서 13개 (65%), SH-2.0IBA 배지에서 14개 (70%), SH-2.5IBA 배지에서 19개 (95%). SH-3.0IBA 배지에서 14개 (70%)의 뿌리유도를 나타내었다.

• 대한미생물학회지
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• 제3권1호
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• pp.5-14
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• 1968

Prostitutes living in Songdo beach in Pusan, for two years period (from September 4, 1964 to August 28, 1966) were investigated for the present status and Venereal Diseases. The following results were obtained. 1. Age distribution:The youngest was 18 years old and the oldest 29. The group between 22-25 years old was 72.8%, approximately three fourth of all prostitute. 2. Educational background: From illiterate to college graduated. The group of graduated primary school was 80.7%, approximately four fifth of all prostitutes. 3. Distribution of birth places: From all cities and provinces in Korea except Chejudo Island province. Kyongsang Namdo province was highest(24%), Kyongsan Pukdo province 18%, Pusan city 12%, and Seoul city 11%. 4. Monthly and weekly Venereal Diseases examination to prostitutes: Total examined cases were 7757, consisting of 323 monthly, and 75 weekly. Total numbers of disqualified persons were 592(7.63% of total examined), consisting of 25 monthly, and 6 weekly. 5. Clinical and laboratory findings of the rejected prostitutes at the Venereal Disease examination: Among total cases of rejected 592, gonococcal positive cases were 2.89%, staining by gram's method, cervical erosions 3.53%, and numerous pus cells but gonococcal negative 1.53%. 6. Seasonal distribution of prostitutes : Songdo beach is one of the famous sea shore sightseeing resort in Pusan. Therefore, numbers of prostitutes were increasing in hot season, summer and decreasing in cold season, winter. 7. Number of disqualified prostitutes at the Venereal Disease examination by seasonal trend: In autumn 18.04%, winter 8.68%, spring 5.85%, and summer 4.43%, in order as described, the decreasing tendency was observed. 8. Serolgical tests for syphilis: VDRL slide method and Kolmer-Wassermann reaction were performed to the prostitutes. STS were positive by VDRL slide method in 8.33-33.87%, Kolmer-Wassermann reaction in 8.33-27.42% of the prostitutes. The results of sero-positive of syphilis showed tendency toward gradually increase from 1964 to 1966. 9. Authors were observed only 1 case or soft chancer, but neither lymphogranuloma venereum nor granuloma inguinale were detected.

• Korean Journal of Acupuncture
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• 제25권3호
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• pp.1-14
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• 2008

Objectives : Weights and measures (度量衡) in Korean traditional medicine have been changed in many dynasties and countries. Thus, measurements of chon (寸) and cheok (尺) have been considered as symbolical meaning in recent studies on meridians and acupoints. The present study was made to settle up standard measurement by surveying length of each parts of upper limb as follows. Methods : To calculate length of 1 chon (寸), the length of each part was divided into the numbers of chon (寸) as shwon in ${\ll}$Goldo Hwangjenaegyeong-yeongchu (靈樞 骨度篇)${\gg}$; the chon (寸) length in ${\ll}$Goldo Hwangjenaegyeong-yeongchu (靈樞 骨度篇)${\gg}$ was compared with the chon (寸) length which is calculated by dividing human average hight into 75 chon. Results : Increment of length of 1 chon (寸) did not change as increment of height in subjects. Length of 1 chon (寸) in height is representative of length of 1 chon (寸) in each parts of upper limb. Conclusions : We suggest that most suitable length of 1 chon (寸)for the standard measurement of acupoint location is 2.20 ${\pm}$ 0.17 cm based on length of elbow joint to wrist joint.

• 한국임상수의학회지
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• 제19권4호
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• pp.429-432
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• 2002

만성 림프구성 백혈병은 오랜기간에 걸쳐서 발전하는 전신질환이며, 급성 백혈병에서 보이는 것보다 더욱 성숙되고 잘 분화된 림프구가 혈액과 골수에 존재하는 것을 특징으로 한다. 본 증례보고에서는 발작, 복수의 임상 증상과 전염성 성병성 종양에 대한 치료경력이 있는 2년령의 거세된 잡종견을 다루고 있다. 여러 가지 진단 절차를 통하여 만성 림프구성 백혈병으로 진단내렸고, chlorambucil과 prednisolone을 이용한 항암치료를 현재까지 적용해 오고 있다. 초기내원 시 보였던 대부분의 증상에 대한 개선을 볼 수 있었으며, 환자의 삶의 질은 향상되었다.

• 한국초지조사료학회지
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• 제21권3호
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• pp.163-168
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• 2001

"Hwasan 103" is a new cold-tolerant Italian ryegrass(Lo1ium multiflorum L.) variety developed by theNational Livestock Research Institute(NLR1) in 1999. To develop a cold-tolerant variety of Italianryegrass(Lo1ium multiflorum L.), cold-tolerant clones survived under - 13 - - 14$^{\circ}$C of minimum average airtemperature(MAAT) in January were selected at Hwachun, Kwangwon Province in 1995. Five clones ofselected clones were polycrossed for seed production by NLRI, RDA in 1996."Hwasan 103" was diploid variety, green in leaf color and broad in flag leaf width. Also it hassemi-prostrate and medium growth habit in late autumn and in early spring, respectively. "Hwasan 103" wasexcellent in lodging tolerance at harvesting time. First heading date of "Hwasan 103" was 14th May, it wassimilar to that of Marshall. Expecially, "Hwasan 103" survived about 50-70% under - 10- - 12'C ofMAAT in January, so it could be cold-tolerant variety that can be safely cultivated in regions higher than -9$^{\circ}$C of MAAT in January. Compared with Marshall "Hwasan 103" showed 3% higher fresh yield (57.8MTper ha) and it showed 2% higher dry matter yield (11.OMT per ha). Higher IVDMD and TDN and lowerADF and NDF were observed in "Hwasan 103" than those of Florida 80 and Marshall in Forage quality.(Key words : Italian ryegrass, Cold tolerance, New variety "Hwasan 103")all in Forage quality.(Key words : Italian ryegrass, Cold tolerance, New variety "Hwasan 103")n 103&")ot;)

• 한국초지조사료학회지
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• 제21권3호
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• pp.151-156
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• 2001

내열성 유전자인 BcHSP17.6를 갖도록 제작한 발현벡터 pBKH4를 Agrobacterium tumefaciens LBA 4404에 도입후, Agrobacterium과 알팔파 캘러스의 공배양을 통해 감염시킨 캘러스를 $100{\mu}g/m{\ell}$의 kanamycin과 $500{\mu}g/m{\ell}$의 cefotaxim을 첨가한 SH-kc배지에서 배양하며 형질전환된 캘러스를 선발하였다. 식물체 재분화는 SH- nk-c, SH-sp-c, SH-11b-c, SH-1BA 배지에서 약 4개월간 배양하여 재분화를 완성하였으며, 재분화된 알팔파의 genomic DNA를 분리한 후, PCR 분석 및 Southern blot 분석을 실시하여 알팔파의 형질전환을 확인하였다.