• Applied Microscopy
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• v.39 no.2
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• pp.117-124
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• 2009

Clonorchis sinensis is the most important widely distributed parasite of the human bile duct in East Asia and the most prevalent parasitic helminth in Korea. The prevalence rate of human clonorchiasis has remained at about 2.9% in Korea. C. sinensis induces dilatation of the duct, hyperplasia of the mucosa, metaplasia or neoplasia of the mucosal epithelium, periductal inflammation and fibrosis, and thickening of the ductal wall. Fibroblast are the most common cells in connective tissue and are responsible for the synthesis of extracellular matrix components. The fibrosis associated with chronic inflammation and injury may also contribute to cholangiocarcinoma pathogenesis, particularly through an increase in extracellular matrix components, which participate in the regulation of bile duct differentiation during development. In this study, ultrastructural changes, the distribution of lectin receptors and actin protein in cultured SD rat bile duct fibroblast after infection of C. sinensis were observed. Experimental group had been divided into four groups: normal bile duct fibroblast cultured in basal media (G1); C. sinensis infected bile duct fibroblast cultured in basal media (G2); normal bile duct fibroblast cultured in basal media containing excretory-secretory product (ESP) (G1-1); C. sinensis infected bile duct fibroblast cultured in basal media containing ESP (G2-1). Overall, once a host is infected by C. sinensis, it affects the host to the extent that sialic acid of ductal fibroblast is increased. Number of cytoplasmic process of SD rat bile duct fibroblast was increased. Actin protein and sialic acid were located in cell surface. Fibroblast induced by C. sinensis was not recovered to normal fibroblast. The cytoplasm bulk and cytoplasmic process were increased whereas the growth rate of the fibroblast of infected SD rat was reduced rather than that of normal fibroblast. In result, it inhibits fibroblast proliferation and increases actin protein on fibroblast cytoplasm, and so causes fibroblast metamorphosis and cellular mutation.

• Development and Reproduction
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• v.4 no.1
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• pp.67-77
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• 2000

Snailfish usually lives at the bottom of the sea and showed typical retrogressive change with specialized tissue structures of skin and skeletons. In order to obtain the specific genes of snailfish, highly expressed in the body, we made subtracted cDNA library and analyzed 200 clones. Totally 200 clones were obtained and sequenced, and among them 62 clones were turned out to be homologous to the known gene, i.e., thioesterase (9), myosin (8), creatine kinase (7), skeletal alpha-actin (6), parvalbumin b (5), ribosomal protein (5), type I collagen (3), muscle troponin (3), dopamine receptor (2), histatin (2), and heat shock protein (2), cystatin (1), lectin (1), statherin (1), secretory carrier membrane protein (1), keratin type I (1), desmin (1), chloroplast (1), muscle tropomyosin (1), reticulum calcium ATPase (1), ribonucleoprotein (1). The remaining 138 clones were low homologous or non-redundant genes through Genbank search. Especially 5 clones were novel and specifically expressed in the body tissues of Snailfish by in situ hybridization. Therefore, we analysed these 5 clones to identify the C-terminal protein structures and motifs, and partly defined the roles of these proteins in comparison with the expression patterns by in situ hybridization. C9O-77, about 5000 bp, was supposed to be a matrix protein expressed strongly positive in epithelium, myxoid tissue, fibrous tissue and collagenous tissue. C9O-116, about 1500 bp, was supposed to be a transmembrane protein which was weakly expressed in the fibrous tissue, epithelium tissue, and myxoid tissue, but strong in muscle tissue. C9O-130, about 1200 bp, was supposed to be an intracytoplasmic molecule usually in the epithelial cells. C9O-161, about 2000 bp, was weakly expressed in epithelium, muscle tissue and myxoid tissue, but specially strong in epithelium. C9O-171, about 1000 bp, was supposed to be a transcription factor containing zinc finger like domain, which was intensely expressed in the epithelium, muscle tissue, fibrous tissue, and in collagenous tissue.

• The Journal of the Korean bone and joint tumor society
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• v.10 no.2
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• pp.130-133
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• 2004

Low-grade fibromyxoid sarcoma (LGFMS) is very rarely seen, and it commonly arises from the deep soft tissues of the lower extremities. Histologically, it is characterized by the presence of bland spindle cells with mainly storiform pattern of growth, set in alternating areas with a myxoid or fibrous stroma. The immunohistochemical reaction of this tumor permits a positive diagnosis of low grade fibromyxoid sarcoma and allows its distinction from a number of other benign and malignant soft tissue tumor. We report this rare case together with the review of the literature.

• Journal of Chest Surgery
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• v.35 no.8
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• pp.638-641
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• 2002

Low grade fibromyxoid sarcoma is a recently recognized, uncommon soft tissue neoplasm. It has a tendency to develop in deep soft tissue of young adults and a possibility of local recurrence or distant metastasis. Diagnostic criteria have not been well defined and this tumor has not been accepted as a distinct entity. Histologically, it is characterized by the presence of bland spindle cells with mainly whorled pattern of growth, set in alternating areas with a myxoid or fibrous stroma. Careful consideration of the morphological and immunohistochemical features of this tumor permit a positive diagnosis of low grade fibromyxoid sarcoma and allow its distinction from a number of other benign and malignant soft tissue neoplasms. We experienced a low grade fibromyxoid sarcoma in chest wall and report this case with a review of the literature.

• Journal of Chest Surgery
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• v.36 no.2
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• pp.113-117
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• 2003

Idiopathic mediastinal fibrosis is very rare. We report two cases of a 41-year-old man and 65-year-old man who presented with backache and vocal cord palsy, subsequently confirmed to be idiopathic mediastinal fibrosis. Preoperative chest computed tomography showed a mediastinal mass and thoracoscopic biopsy was preformed. The mass was hard, dense and partially calcified, and adhered the adjacent mediastinal structure. Postoperative medical treatment was not performed, and during 5 and 7 month follow-up has not demonstrated any complication.

• Investigative Magnetic Resonance Imaging
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• v.13 no.2
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• pp.203-206
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• 2009

Fibrous dysplasia is a slowly progressive, benign disorder characterized by fibrous tissue replacement of skeleton and may affect solitary or multiple bones. Monostotic fibrous dysplasia mainly occurrs in the rib, femur and tibia, however, rarely in the hand. We report a case of monostotic fibrous dysplasia confined to the 2nd metacarpal bone with findings of plain radiographs and MR imaging.

• Journal of the korean academy of Pediatric Dentistry
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• v.38 no.3
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• pp.296-302
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• 2011

Idiopathic gingival fibromatosisrarely occurs, but frequently recurred after surgical removal. It usually occurs in generalized symmetrical pattern but sometimes in localized unilateral pattern. The localized pattern usually affects the maxillary molar and tuberosity area. This disease usually causes tooth migration, malocclusion, and problems in eating, speech, and esthetics. A boy showed dense gingival fibromatosis localized at primary maxillary right lateral incisor area at the age of 5 years, and his maxillary right lateral incisor become severely displaced at the age of 9 years. He had no medical and hereditary factors relevant to the gingival fibromatosis. However, the dense fibrous tissue was dominant in his labial gingiva of maxillary right incisors. In order to realign the displaced incisors by orthodontic treatment, the dense fibrous tissue covered the defect space between the central incisor and the displaced lateral incisor was surgically removed. The removed specimen was examined by simple immunohistochemical(IHC) array method. IHC array showed increased expression of CTGF, HSP-70, MMP-1, PCNA, CMG2, and TNF-${\alpha}$ in keratinocytes, fibroblasts, endothelial cells, and macrophages of gingival fibromatosis tissue. Therefore, it was suggested that the gingival fibromatosis be caused by the concomitant overexpression of CTGF, HSP-70, MMP-1, PCNA, CMG2, and TNF-${\alpha}$, and resulted in the fibroepithelial proliferation and the inflammatory reaction of gingival tissue.

• Journal of Periodontal and Implant Science
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• v.25 no.1
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• pp.99-110
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• 1995

치주치료의 궁극적 목표인 치주조직 재생에 대한 관심이 높아지는 가운데 치주조직 재생에 주된 역할을 하는 치주인대세포와 치근면 탈회에 의한 신부착 효과에 관한 많은 연구가 시도되고 있다. 그러나 광범위한 연구에도 불구하고 어떤 치근면 처리가 신부착 형성에 가장 효과적인지는 아직 논란의 대상으로 남아있다. 이에 저자는 결합조직 부착에 의한 재생에 필수적인 치은 및 치주인대 섬유아세포의 부착 및 증식을 tetracycline-HCL(TC)과 citric acid(CA)로 각각 처리된 상아질 표면에서 비교관찰하여 치주조직 재생에의 기여도를 추정하기 위해 본 연구를 시행하였다. 교정치료를 위하여 본원에 내원한 환자의 제일 소구치의 건강한 치은조직을 채취하고, 발치 후 효소처리에 의한 치주인대세포를 얻은 후 각각 세포배양하였다. 상아질 절편은 $3{\times}3{\times}0.2mm^3$로 준비하고 TC과 CA처리군으로 나눈 후 각각 치은 및 치주인대 섬유아세포를 부착시키고 초기부착은 8시간까지, 증식은 7일까지 고나찰하여 다음의 결과를 얻었다. 1. 각 군의 세포 부착은 시간이 지남에 따라 증가되었다. 2. 모든 군에서 7일째 빠른 증식상을 보였다. 3. 초기 부착시 치은과 치주인대 섬유아세포의 반응에는 유의한 차이가 없었다. 4. 반면에 치은과 치주인대 섬유아세포는 증식속도에서 차이를 나타내었으며 치은 섬유아세포가 좀 더 빠른 성장을 보였다. 5. 치근면 탈회에 따라 초기부착과 증식상에서 모두 유의성 있는 증가를 보였다. 6. CA는 초기부착에서, TC는 증식장에서 더욱 효과적으로 작용했다.

• Journal of the korean academy of Pediatric Dentistry
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• v.28 no.1
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• pp.171-174
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• 2001

This case describes a case of desmoplastic fibroma of the mandible. A 9-year-old boy was seen with a history of progressive swelling and expansion of the left mandible for one month period. Desmoplastic fibroma was diagnosed on histopathologic examination This report reviews the diagnostic criteria, differential diagnosis and surgical treatment of choice in brief of this uncommon primary bone tumor of the oral and maxillofacial region.

• 한국전자현미경학회:학술대회논문집
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• 2007.05a
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• pp.71-74
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• 2007

천연 대마섬유의 UVB 차단 효과를 조사하고자 mouse를 4개의 그룹으로 구분하여 자외선을 조사한 뒤 외이의 조직절편을 제작하여 비교 분석하였다. 자외선을 직접 조사한 실험군[U], 대마섬유로 차단한 뒤 자외선을 조사한 실험군[H], 폴리에스테르 섬유로 차단한 후 자외선을 조사한 실험군[P], 자외선을 조사하지 않은 대조군[C]으로 나누어 실험한 후 광학현미경하에서 피부 조직의 변화를 관찰하였다. 대조군[C] 외이조직의 상피세포층 두께는 $16.3\;{\mu}m$이었지만, 자외선을 직접 조사한[U]군의 상피세포층은 $65.4\;{\mu}m$, [H]군은 $33.3\;{\mu}m$, [P]군은 $41.0\;{\mu}m$의 두께 변화를 보였다. 또한, 정상적인 외이의 상피세포의 단면적은 $22.0\;{\mu}m^2$이었으나, 자외선을 직접 조사한 [U]군의 상피세포층은 $37.7\;{\mu}m^2$, [H]군은 $30.1\;{\mu}m^2$, [P]군은 $30.2\;{\mu}m^2$이었다. 따라서 천연 대마섬유와 합성 폴리에스테르섬유 모두 UVB를 차단하는 능력을 갖고 있지만 전자가 후자보다 더 효과적으로 UVB를 차단함을 알 수 있었다.