• Title/Summary/Keyword: 선충중

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Studies on the Pathogenicity of Aphelenchoides sp. and Rhabditis sp. attacking Cultivated Mushroom, Agaricus bisporus(Lange) Sing (Aphelenchoides sp.와 Rhabditis sp.의 양송이에 대한 병원성에 관한 연구)

  • Han, Yong-Sik;Park, Jung-Su;Kim, Dong-Soo;Shin, Kwan-Chul
    • The Korean Journal of Mycology
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    • v.1 no.1
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    • pp.3-11
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    • 1973
  • Four species of nematodes attacking mushroom beds were found in samples taken from 35 mushroom farms throughout Korea. These were Rhabditis sp., Aphelenchoides sp., Ditylenchus sp. and Aphelenchus sp. ,Rhabditis sp. was found from compost and casing from all mushroom farms and the frequencies of Aphelenchoides sp. was 31.4% in the both compost and casing. Both Ditylenchus sp. and Aphelenchus sp. showed 2.7% of frequencies in the compost, none in casing. Temperature and moisture content of compost affected pathogenicity of Aphelenchoides sp. on mushroom mycelia grown in compost. The higher temperature and moisture content the sooner the damage became apparent, and the more rapid was subsequent destruction of mycelia. There was no mycelial destruction at the lowest temperature of $10^{\circ}C$. Rhabditis sp. completely disintegrated mycelia grown in the compost, in the early stage, the numbers of Rhabditis sp. rose gradually and then increased suddenly to reach a peak but soon declined. At first, the pH of Rhabditis-infested spawned compost declined but then rose gradually as mycelia was disintegrated by nematodes. The trend in pH of infested unspawned compost was similar to those of uninfested, unspawned compost. Cultures inoculated with surface-disinfected dead Rhabditis sp. and with tap water used in the nematode extraction procedures showed no mycelial injury associated microorganisms containing within or outside the nematodes even though added by artificial wounding of the mycelia. Cultures artificially wounded showed no injury away from the wounds without the presence of living Rhabditis sp., such wounded mycelia slowly regenerated. On the other hand, artificial wounding accelerated the breakdown of mycelia in the presence of living Rhabditis sp.

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Parachordodes megareolatus, a New Species of Horsehair Worm(Nematomorpha: Gordioida: Gordea) from Korea (한국산 철선충류 (유선형동물문: 연가시강: 연가시목)의 1신종, 진주철선충)

  • Andreas Schmidt-Rhaesa;Pyung-Rim Chung;Woon-Mok Sohn
    • Animal Systematics, Evolution and Diversity
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    • v.19 no.2
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    • pp.161-166
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    • 2003
  • One new species of horsehair worms (Nematomorpha) from Korea, Poruchordodes megareolatus, is described. The specimen is reported from the human urinary system. The significance of this and other reports from the urinary system is discussed. The characteristics of the new species are clusters of a small type of cuticular structures, the areoles, which are associated with a second type of areoles, the large megareoles. Another character unique for the genus Parachordodes is the fusion of rows of cuticular bristles on the ventral side anterior of the cloacal opening.

Selection of Soybean Cultivars Resistant to Soybean Cyst Nematode, Heterodera glycines (콩씨스트선충(線蟲) 저항성품종(抵抗性品種) 육성(育成)에 관(關)한 연구(硏究))

  • Choi, Y.E.;Kim, D.G.;Choi, D.R.
    • Korean journal of applied entomology
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    • v.25 no.1 s.66
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    • pp.53-61
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    • 1986
  • The races of soybean cyst nematode, Heterodera glycines were investigated at 13 localities in Kyungpook, Chungpook and Cheonnam provinces for the resistant soybean variety selection to soybean cyst nematode. Race C, 1, and 5 were distributed and among them race 5 was dominant in Korea. Native soybean 203 lines and 25 introduced soybean varieties were investigated in field where soybean cyst nematode race 5 infested. As the result, the reproduction of soybean cyst nematode in native soybean lines decreased on entry number 18, 21, 36, 38, 41, 42, 43, 45, 55, 59, 68, 72, 75, 93, 94, 95, 98, 123, 131, 136, 146, 170, 174, 187, 190, 191 than others. And then, in pot experiment, entry number 170 showed moderately resistant with 10 cysts per root, while entry number 55 was decreased reproduction a little and the other 25 line appeared susceptibility. In introduced soybean varieties, Peking, Illsoy and PI90763 showed resistance and not reproduction in field and pot experiment. But Custer, Delmar, Dyer and Jackson represented moderately resistant under 10 cysts, while the other 18 varieties were susceptible. In yield analysis, native soybean line 170 showed the same tendency as Peking and PI90763 known for resistant varieties in decreased rate of branch number, pod number, pod weight per plant and weight of 100 grains. Peking and PI90763 decreased at the rate of under 1.6% in branch number, pod number, pod weight per plant and weight of 100 grains, while Illsoy showed a little higher decreased rate of 3.8% in pod number and 3.4% in pod weight per plant. Custer, Delmar, Dyer and Jackson represented a little higher decreased rate in yield.

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Effect of the Density of Heterodera glycines on Soybean Yield (콩씨스트선충(Heterodera glycines)의 밀도가 콩 수량에 미치는 영향)

  • Kang, Heonil;Park, Byeongyong;Park, Sekeun;Kim, Eunwha;Park, Eunhyeong;Ko, Hyoung-Rai
    • Korean Journal of Plant Resources
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    • v.34 no.4
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    • pp.257-262
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    • 2021
  • This study was conducted to investigate the yield loss of soybean by initial density of Heterodera glycines in soil. A soybean cultivar named 'Cheongja5ho' was cultivated as test material in Dangjin city, Chungcheongnam-do, Korea. Each seed was planted with distance of 25 cm in 35 plots by initial density of egg of the cyst nematode on June 21st, and allowed to harvest on November 12st in 2019. As a results, the yield of soybean decreased sharply when as the initial density of eggs of cyst nematode increased. The initial density of eggs in soil affected largely on the decreased yields of the soybean by more than 20% in above 2,000 eggs, and 30% in above 6,000 eggs of the cyst. Beyond the 6,000 eggs, the yield loss was more slowly decreased compared with the above cases. Eggs of H. glycines are widely distributed in Korea, resulting in the serious soybean yield loss by H. glycines.

Identification of Quantitative Trait Loci for Resistance to Soybean Cyst Nematode Race 5 (콩 Cyst 선충 Race 5에 대한 저항성 QTL 탐색)

  • Choi, In-Soo;Kim, Yong-Chul;Kim, Sung-Man;Lee, Chung-Yeol;Park, Hyean-Cheal;Halina T. Skorupska
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.42 no.6
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    • pp.712-721
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    • 1997
  • The objectives of this study were; (1) to identify and localize QTLs for resistance to soybean cyst nematode(SCN) race 5 on RAPD map, (2) to idntify the magnitude and mode of inheritance for each QTL, and (3) to identify the best combinations of QTLs for resistance to SCN race 5. Based on the univariate regression analysis, we detected 26 markers(22 RAPD and 4 RFLP) which showed significant association(P<0.05) with resistance to SCN race 5. From MAPMAKER /QTL analysis, we identified two regions (LGC-20 and Group 2) for resistance to SCN race 5. The QTL that was localized at 8.0 cM from pK418C on LGC-20 showed a recessive mode of inheritance and the QTL that was localized between W03 and E02$^3$ on Group 2 showed a dominant mode of inheritance. Two pairs of flanking markers (E02$^3$ and W03, pK418C and pK418E$_1$) and one unlinked RAPD marker, G10$^1$ were used for multiple regression analysis. Marker combination which was composed of 4 markers, E02$^3$, G10$^1$, W03, and pK418E$_1$, explained the highest amount of phenotypic variation by SCN (35.2%). Further research for the identification of QTLs for resistance to SCN race 5 to explain larger portion of phenotypic variation is needed.

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Effect of Neem and Mustard oils on Entomopathogenic Nematodes and Silkworm (Neem과 mustard oil이 곤충병원성 선충과 누에에 미치는 영향)

  • Ha, Pan-Jung;Kim, Tae-Su;Lee, Shin-Hae;Choo, Ho-Yul;Choi, Sung-Hwan;Kim, Young-Sub;Lee, Dong-Woon
    • The Korean Journal of Pesticide Science
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    • v.14 no.1
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    • pp.54-64
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    • 2010
  • Effects of thirteen essential oils (anise oil, clove oil, marigold, mustard oil, neem oil, quassia, quilaja, rosemary oil, rotenone, tea tree extract, thyme oil, wintergreen oil, and yucca) and caffeine on typical industrial insect, silkworm (Bombyx mori) and two entomopathogenic nematodes, Steinernema carpocapsae GSN-1 strain (Sc) and Heterorhabditis sp. Gyeongsan strain (Hg) were investigated in the laboratory. When 1,000 ppm of each essential oils was treated, neem oil showed the highest insecticidal activity against silkworm. Mortality of silkworm fed on neem oil treated mulberry leaf was 55.3 and 100% 5 and 10 days after treatment, respectively. The silkworm fed on neem oil treated mulberry leaf did not make cocoon and pupa. Weight of cocoon and pupa was low in rotenone treatment showing 0.27 g and 1.01 g, respectively. Mustard oil had the highest nematicidal activity against entomopathogenic nematodes. 20 ppm of mustard oil resulted in 69.0% and 100% mortality of Sc and Hg 3 days after treatment, but 4% and 36% at 5 ppm in X-plate, respectively. Mortality of baited Galleria mellonella larva by Sc was not different from control at the concentration of 100 ppm of mustard oil while 30% lower in Hg in sand barrier. Mean numbers of established infective juveniles of Hg in Galleria larva were lower than Sc in sand barrier. Survival rate of Sc was similar to control at the concentration of <200 ppm of mustard oil in sand barrier.

Resistance of Commercial Pepper Cultivars to Root-knot Nematodes (시판 고추품종에 대한 뿌리혹선충 저항성 검정)

  • Kim, Donggeun;Kwon, Taeyoung;Ryu, Younghyun;Yeon, Ilkwon;Huh, Changseok
    • Research in Plant Disease
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    • v.18 no.4
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    • pp.370-375
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    • 2012
  • Ninety two pepper (Capsicum annuum L.) cultivars were screened for resistance to two common species of root-knot nematodes, Meloidogyne incognita and M. arenaria. All 92 pepper cultivars were resistant to M. arenaria (0-3 eggmass/plant) but were susceptible to M. incognita (76-678 eggmass/plant). Susceptibility to M. incognita were slightly differed; 'Geomok' and 'Shintaepung' had relatively less eggmass (<100 eggmass/plant) when compared to those 29 very susceptible culivars such as 'Bulggotcheoreum' (>300 eggmass/plant). Therefore, pepper is highly recommended as a high-valued rotation crop to only those greenhouses infested with M. arenaria, but should restrict for M. incognita.

Growth Optimization of Photorhabdus luminescens Isolated from Entomopathogenic Nematode Heterorhabditis bacteriophora (병원성 선충 Heterorhabditis bacteriophora에서 분리된 공생 박테리아 Photorhabdus luminescens의 생장조건)

  • Yoo, Sun Kyun;Randy Gaugler;Christopher W. Brey
    • Microbiology and Biotechnology Letters
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    • v.29 no.2
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    • pp.104-109
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    • 2001
  • The yield of infective juveniles of Heterorhabditis bacteriophora (Tf strain) in vitro monoxenic liquid culture was improved significantly as the amount of symbiont biomass, Photorhabdus sp. strain Tf, increased. To investigate the influence of abiotic factors on the growth and biomass production of Photorhabdus sp. strain Tf, triplicate flask cu1tmes were performed. The optinal temperature and medium pH for the growth of Photorhahdus sp. strain Tf were 30$^{\circ}$C and between pH 5.5-7.3, respectively. Aeration also improved greatly growth and yield of biomass of Photorhabdus sp. strain Tf. Photorhabdus sp. strain Tf in batch fermentation showed growth-associated pattem in terms of pigment production, and the pH of culture medium rose steadily until growth stopped dUling the fermentation. Both pigment production and culture pH rise would be useful parameters indicating a reliable growth of Photorhabdus sp. strain Tf.

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Development of a Biocontrol Agent Using Monacrosporium thaumasium to Control a Root Knot Nematode, Meloidogyne incognita (Monacrosporium thaumasium을 이용한 뿌리혹선충 (Meloidogyne incognita) 방제용 미생물제제의 개발)

  • Choi, Ye-Hoon;Kim, Keun-Ki;Son, Hong-Joo;Shin, Hae-Soo;Park, Hyean-Cheal
    • Journal of Life Science
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    • v.17 no.12
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    • pp.1605-1609
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    • 2007
  • The created microbial pesticide was used to examine its effects using the inside-pot test method. The selected microbial pesticide KBC3017 particle pesticide manufactured by using Monacrosporium thaumasium was used in the farm-house outdoor test to find the optimum consistency and its effects. The more amount used, the better effect it showed. However, the optimum consistency was 2% and the KBC3017 particle pesticide for which the diatomite and raw jade powder were used as an increaser, when used 2% level of the total amount of soil, showed 71% effect on nematode prevention. The root and the stem of crops were better compared to those without any pesticide used.

Screening of Tomato Cultivars Resistant to Root-knot Nematode, Meloidogyne incognita (고구마 뿌리혹선충 저항성 토마토 품종 스크리닝)

  • Kim, Hyeong-Hwan;Cho, Myoung-Rae;Kang, Taek-Joon;Jung, Jae-A;Han, You-Kyoung
    • Research in Plant Disease
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    • v.16 no.3
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    • pp.294-298
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    • 2010
  • Thirty-two tomato cultivars, eleven cherry tomato cultivars and eight rootstock tomato cultivars were inoculated with Meloidogyne incognita, to evaluate tomato cultivars for resistance against root-knot nematodes. One tomato cultivar, Homerunking was proved to be resistant while eight cultivars such as Regendsummer, Regend, Sunmyung, Pinktop, Top3, Delice, Tasha and Lilyance were moderately resistant to the root-knot nematodes. Five cherry tomato cultivars (Tenten, Desert, Redstar, Veryking and Arigatto) showed moderate resistance and one cultivar (Redcherry) was resistant to root-knot nematodes. In eight rootstock tomato cultivars, two cultivars (Bukinghagae, Special) were resistant and five cultivars (B-blocking, Solution, Dongbanja and Greenpower) were moderate.