• Korean journal of applied entomology
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• v.52 no.4
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• pp.327-333
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• 2013

Plant parasitic nematodes were isolated from 274 soil samples collected from soybean fields in Korea. Nematode importance rating in soybean is proposed based on this study and by reviewing other reports. Soybean cyst nematode, Heterodera glycines is the most important nematode species and rated as $1^{st}$ because it detected from 38%(range 25-51%) of soil samples with high density except Jeju province. Root-knot nematode, Meloidogyne spp. is rated $2^{nd}$ and is more widely distributed in southern provinces, Gyeongnam, Jeonnam, and Jeju province (detection rate ranged 16-44%). Pratylenchus is rated $3^{rd}$ and is more frequently detected from northern provinces such as Gyeonggi and Gangwon (detection rate ranged 10-13%). Helicotylenchus is rated $4^{th}$ and is particularly important in Jeju province (detection rate is 62% and numbers averaged 571 nematodes/$300cm^3$ soil), which is the main production area for sprouting soybean in Korea. Tylenchorhynchus, Paratylenchus, Criconema, Criconemoide, Mesocriconema, Ogma, Xiphinema, Paratrichodorus, and Trichodorus occur in low frequency and density, thus they are rated to $5^{th}$. We propose to delete following nematode genus from the list of soybean parasitic nematode in Korea because their parasitism on soybean is unconfirmed or negligible; Tylenchus, Aphelenchus, Aphelenchoides, Ditylenchus, Hirschmanniella immamuri, Basiria graminophila, Psilenchus, and Pseudhalenchus. Therefore, we revised 30 species in 16 genera as soybean parasitic nematodes in Korea. Importance rating is $1^{st}$ Heterodera glycines, $2^{nd}$ Meloidogyne spp., $3^{rd}$ Pratylenchus spp. and $4^{th}$ Helicotylenchus spp. especially in Jeju province.

• Korean journal of applied entomology
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• v.25 no.3 s.68
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• pp.175-182
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• 1986

A study was conducted to survey the fauna and the field density ofp lant parastic nematodes on important cash crops in Korea. Studies were carried out with sampling about five hundred grams of soil around roots of eighteen different kinds of crops from fifty five locations throughout the country. Nematodes were elutriated from over three thousand soil samples, and identified into sixteen different genera. Among them Ditylenchus species were found to be the most abundant in fields of garlic and onion, Helicoty-lenchus of sesame, chinese cabbage and mulberry, Meloidogyne of red-pepper, tomato, cucumber and peanut, Pratylenchus of mulberry and peach, Pratylenchus of apple, Chinese cabbage and radish, Trichodorus of potato, and Xiphinema of peach and potato. Field density of Meloidogyne species was increased with the longer continuous cultivation of red-pepper in the same field.

• Research in Plant Disease
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• v.12 no.2
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• pp.108-114
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• 2006

This study was conducted to elucidate the interactions among soil microorganisms in a special field where one, two or three of N, P, K fertilizers were continuously not applied for 29 years. Crop yield (barley, soybean), soil chemical properties and microflora and microfauna including nematodes, nematophagous fungi, actinomycetes, bacteria, and fungi were examined for two years. Tylenchorhynchus sp. was the most important plant-parasitic nematode (range $11{\sim}642/300 cm^3$ soil) followed by Pratylenchus sp. and Helicotylenchus sp. Among nematophagous fungi, Monacrosporium spp. was the most frequently found followed by Harposporium sp. and Cystopage sp. In general, plots treated with phosphate fertilizer yielded more, had more nematodes, bacteria and actinomycetes. In contrast, total fungal population densities including nematophagous fungi, Cystopage sp. and Harposporium sp. were in reverse; they were more abundant in the plots with lower phosphate contents. Phosphate and pH are positively correlated and two most important determining factors for the population density of soil organisms under investigation. According to correlation analysis, Ca, Mg, and $SiO_2$ contents in soil and population densities of Tylenchorhynchus sp., saprophitic nematodes, actinomycetes, and bacteria were positively correlated with pH, but were negatively correlated with fungal population densities. We hope that the study will add an additional knowledges to understand our mysterious underworld.

• Korean journal of applied entomology
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• v.45 no.2 s.143
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• pp.201-209
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• 2006

Five strains of Korean entomopathogenic nematodes (EPN), steinernematids and heterorhabditids were evaluated and tried in laboratory, pot, and vegetable greenhouses for environmentally friendly control of diamondback moth (DBM), Plutella xyiostella, from 2002 to 2005. LC$_{50}$ values of five EPN strains against DBM were different depending on nematode strain and DBM instar. LC$_{50}$ value of Steinernema carpocapsae GSN1 (GSN1) was the lowest representing 2.6$\sim$3.9 infective juveniles (Ijs, 3rd stage) to 2nd to 4th instars of DBM. Pathogenicity of five effective strains against DBM was different depending on nematode strain, concentration, application times, and vegetable in pot. The most effective nematode was GSN1. Steinernema spp. was more effective than Heterorhabditis spp. against DBM. Two or three times of applications of EPN were effective regardless of nematode strain and concentration in pot. Efficacy of EPN was different depending on vegetable species. Efficacy was higher on Chinese cabbage, red mustard, and Ssamchoo than that on cabbage, kale, and leaf broccoli. Efficacy of GSN1, Steinernema GSNUS-10, Steinernema CSNUS-14, and Heterorhabditis GSNUH-1 was variable depending on nematode strain, concentration, application times, and vegetable in greenhouse experiments. GSN1 was the most effective and 100,000 infective juveniles per m$^2$(=$1\times10^9$ Ijs/ha) resulted in higher efficacy. Three times of application of nematodes led to higher control efficacy than one or two applications. Efficacy of nematodes was higher on Chinese cabbage than cabbage or kale in greenhouse.

• Research in Plant Disease
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• v.18 no.4
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• pp.381-386
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• 2012

Nematode fauna in Dokdo island was investigated for two years between 2008 and 2009. Population density and biomass were $239,500m^2$ (26,000-836,000) and $103.9mg/m^2$ (3.0-388.6), respectively. Total of 31 species of nematodes were identified : Acrobeloides is the most important genus with prominence value (PV) of 117.8, followed by Rhabditidae (PV = 81.9), Prismatolaimus (PV = 39.0), and Aphelenchoides (PV = 31.4). Among feeding group, bacteriovorus nematode composed 77% of density and 62% of biomass followed by plant-parasitic and fungivorus nematodes. A species of Pratylenchus having distinctive tail terminus is under study as a new species. Biological indices, Shannon and Wiener index (2.46), Simpson's diversity index (0.86), Species evenness (0.73), Species richness (1.30) indicate that nematode fauna in Dokdo island is diverse and unique. This study may give a general idea of nematode fauna at the Dokdo island.

• Korean journal of applied entomology
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• v.39 no.2
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• pp.123-129
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• 2000

This study was conducted to analyse the distribution of root-knot nematodes (Meloidogyne spp.) in fruit vegetable production areas in Korea. Soil samples were collected from greenhouses in Sungju (Kyungpook), Yeoju (Kyungki), Haman (Kyungnam), and Chungwon (Choongpook) provinces in 1997-1999. Plant parasitic nematodes were separated for density counting and some of the root-knot nematodes were identified using enzyme phenotypes of malate dehydrogenase (MDH) and esterase (EST). Among the 185 farms in Sungju province, Meloiciogyne spp. were detected from 99 farms (53.5%). Other plant parasitic nematodes detected were; Helic~otylmchuss pp. from 7 farms, Aphelenchus spp. from 43 farms, and Criconematids from 26 farms. Using the female enzyme phenotypes of MDH and EST. the four major root-knot nematodes in Korea, M. incognitc~(M I), M. uretznri~(M~ A), M. huplu (MH), and M. juvunica, could be identified. In the enzyme phenotype identification of 13 populations collected from Sunnam in Sungju province, 6 populations were identified as MA, 5 populations were identified as MI, and 2 populations were mixed with MI and MA. Among the 6 populations from Chojun in Sungju province. 4 populations were MA, one population was MI, and one population showed enzyme phenotypes of unknown species. Among the 14 populations of Yeoju province, l I populations were MH and 3 populations were MA.

• Korean Journal of Environmental Biology
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• v.39 no.2
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• pp.147-155
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• 2021

To investigate the incidence of plant-parasitic nematodes (PPNs) in perilla fields in Korea, 55 soil samples were collected from open fields and plastic-film house fields and were analyzed during January 2020 to October 2020. Root-lesion nematodes (RLNs), spiral nematodes, root-knot nematodes, and stunt nematodes were detected in perilla fields, and the incidences of RLNs (39%) and spiral nematodes (55%) were higher than those of other nematodes. Among PPNs, RLNs are very important species found on economic crops. The detection frequency of RLNs was increased due to continuous crop cultivation, and the frequency of fields that cultivated only one crop continuously for over 11 years was twice higher than the frequency of fields that cultivated only one crop for less than 5 years. The PPN species diversity and density were different between leaf-perilla cultivation fields and seed-perilla cultivation fields. In phylogenetic analysis of RLNs, Pratylenchus penetrans, P. vulnus, and Pratylenchoides leiocauda were identified in perilla fields. These results suggest the RLNs should be considered for establishing nematode management strategies in perilla fields in Korea.

• Korean journal of applied entomology
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• v.45 no.1 s.142
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• pp.79-85
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• 2006

This study was conducted to get basic information on the occurrence of plant-parasitic nematodes for the establishment of nematode management strategy in major potato production areas in Korea. Nationwide soil collection was done in 11 areas of Cheju, Yesan, Gimchun, Goryoung, Hong chun, Pyungchang, Gimjae, Milyang, Namwon, Gangnung, and Inje in 2004-2005. Root-hot nematode juveniles(J2) were detected in 30 samples among the 50 samples. The average density was 12-69 J2/100cc soil. Pratylenchus sp., Helicotylenchus sp., Ditylenchus sp., Tylenchus sp., and Tylenchorhynchus sp. were also detected in various locations, however, their densities were very low. Root-knot nematode females were collected from tomato roots inoculated with the potato field soils for PCR-RFLP identification. The females from Cheju, Milyang, and Goryung showed PCR products of 500 bp. And the Dra I restriction enzyme digestions showing 290 bp and 230 bp fragments confirmed their identity as Meloidogyne hapla.

• Research in Plant Disease
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• v.22 no.3
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• pp.184-189
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• 2016

To select resistant oil seed crops against two species of root-knot nematodes, Meloidogyne incognita and M. arenaria, 10 cultivars of sesame (Sesamum indicum L.) and 10 cultivars of perilla (Perilla frutescens var. japonica) were screened in greenhouse pot test. All sesame cultivars tested were resistant to M. incognita but susceptible to M. arenaria. While, perilla was resistant to both Meloidogyne species. Therefore, perilla cultivars could be used as rotation crop in greenhouse infested with both M. incognita and M. arenaria. But, sesame cultivars only can be used as a rotation crop in greenhouse infested with M. incognita but not for M. arenaria.

• Journal of Applied Biological Chemistry
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• v.55 no.4
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• pp.247-252
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• 2012

This study examined the control effects of amino acid biochemical agent extracted from chicken feathers on Meloidogyne incognita for the purpose of developing an environmentally friendly nematicidal agent that can replace chemical control of root-knot nematodes (RKN). We investigated the lethal effects of J2 juveniles for 19 types of commercial amino acids. As a result, five kinds of amino acids (L-asparagine, L-aspartic acid, L-methionine, L-tyrosine, L-cysteine) showed mortality rate of more than 50% at a concentration of 50 mM. L-asparagine showed the highest mortality rate at 94%. We also investigated the lethal effect of J2 juveniles and suppressive effects of egg hatching by feather amino acids (FAA) biochemical agent. It showed that the mortality rate of J2 juveniles was more than 80% and suppression rate of egg hatching was 74% at 1/50 concentrations of FAA. As a result of conducting a tomato pot culture experiment for 60 days after treating 1/50 concentrations of FAA biochemical agent in rhizosphere soil, it showed that the control effects were 63% of juveniles density in the soil, 59% of egg mass and 61% of root gall index, respectively. Based on the above results, it is considered that the FAA biochemical agent extracted from chicken feathers can be used as an environmentally friendly nematicidal agent of RKN.