• 보건세계
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• v.63 no.1
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• pp.30-32
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• 2014

협회는 국민보건 사업의 일환으로 결핵 및 호흡기질환의 예방과 퇴치를 위해 홍보, 교육 사업은 물론 결핵예방접종 백신 및 감염진단 시약의 생산과 연구 등과 같은 다양한 사업을 수행해오고 있다. 그 가운데에서도 이번 호에서는 결핵 발견의 최전방에 선 검진팀의 일과를 소개하고자 한다.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.18 no.9
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• pp.123-126
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• 2017

Mumps, which are also known as epidemic parotitis,lead to viral infectious diseases that can cause complication such as pancreatitis, orchitis, hearing impairments, oophoritis, parotitis and meningitis. Central nervous system involvement has been reported in up to 65% of mumps patients, with most of these including meningitis. Meningoencephalitis is a rare central nervous system (CNS) complication of the mumps. Measles-Mumps-Rubella (MMR) vaccination has been reported protective effect against clinical complications and hospitalization. Here, we describe acute mumps meningoencephalitis in a 24-year-old female without parotitis who had received the MMR vaccination by age related schedule. She initially visited our hospital with aggravated cognition and confusion. After conservative viral treatment, she recovered cognitive function rapidly and complication was not remained. We report here a case of a patient with good recovery of mumps meningoencephalitis without parotitis after secondary vaccination for MMR.

• Research in Plant Disease
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• v.12 no.3
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• pp.298-302
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• 2006

An isolate of Cucumber mosaic virus(CMV), called as Can-CMV, was originally isolated from Canna generalis showing typical streak mosaic foliar symptoms, and its properties were investigated in this study. Whereas all known isolates of CMV could induce symptoms on their systemic hosts(four kinds of Nicotiana spp and a zucchini squash), Can-CMV induced no symptoms on its systemic hosts tested. Replication and movement of the virus on upper leaves as well as inoculated leaves-were confirmed by RT-PCR suggesting that Can-CMV could only infect systemically on N. benthamiana and N. glutinosa. Size of local lesions on the Can-CMV-inoculated leaves of Chenopodium amaranticolor was much smaller than that of Fny-CMV. Whereas Fny-CMV and LS-CMV could induce distinct necrotic local lesions on Vigna unguiculata 2 to 3 days postinoculation(dpi), chlorotic spots symptom was expressed by Can-CMV 4 to 5 dpi. Virus-specific 4 kinds of dsRNAs were isolated from leaves of N. benthamiana infected with Can-CMV, and these dsRNAs corresponded to the viral genomic RNAs and subgenomic RNAs and their patterns were indistinguishable to those of Fny-CMV and LS-CMV. By restriction mapping analysis of 950 bp of RT-PCR amplified products of coat protein gene of the virus as well as by serological analysis of gel diffusion test, Can-CMV belongs to a typical member of CMV subgroup IA. These results suggest that the Can-CMV isolated from C. generalis possesses unique pathological properties to understand further insight into the various interactions between virus and host.

• Korean Journal of Soil Science and Fertilizer
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• v.32 no.1
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• pp.68-75
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• 1999

This study was conducted to examine the effects of Arbuscular Mycorrhizae inoculation and phosphorus application on early growth of hot pepper. Gigaspora margarita and Acaulospora spinosa were chosen for this investigation and inoculated into soils of different P levels by varying inoculation time and density. After treatment, some relevant growth responses of hot pepper were measured. Regardless of soil P levels, hot peppers treated with arbuscular mycorrhizal fungi had 5~34% more fresh weight than those untreated, but the effect of inoculation time and density was not different between two species. With decreased P levels, the infection rate and dependency of hot peppers increased. The content of P and K of AMF-inoculated hot peppers increased with increasing P levels, but the shoot to root ratio of those elements decreased. The results of this study showed that inoculation of AMF would be effective in promoting growth of hot pepper seedlings and increase transplant adaptation due in part to the resulted higher root development.

• Journal of the Korean Society of Food Science and Nutrition
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• v.43 no.5
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• pp.756-762
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• 2014

This study evaluated the quality characteristics of soybean fermented with several Bacillus sp., which were selected based on their high enzymatic and antimicrobial activities. Total aerobic bacterial counts of fermented soybeans with HJ5-2 ($3.00{\times}10^9CFU/mL$) were the highest among all strains. Lactic acid bacteria numbered $2.50{\times}10^2{\sim}7.30{\times}10^4CFU/mL$ in soybeans fermented with isolates. Amylase and protease activities of the RD7-7 sample were the highest among all strains. Reducing sugar and amino-type nitrogen contents of fermented soybeans with HJ18-4 (2.35%) and RD7-7 (227.96 mg%) were the highest. Total amino acid contents of the samples were 16.62~18.38%, and glutamic acid, aspartic acid, leucine, lysine, arginine were major amino acids. Oxalic acid (36.51~63.57 mg/100 g) and succinic acid (429.49~600.15 mg/100 g) were the predominant organic acid. These results provide useful information for development starter (single and complex) as well as for the production of high quality fermented soybean foods.

• The Korean Journal of Mycology
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• v.9 no.1
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• pp.1-6
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• 1981

The effect of gamma irradiation on production and accumulation of aflatoxin on natural substrate (barley) by Aspergillus parasiticus NRRL 2999 has been studied in some detail. Gamma irradiation at five doses, 0, 50, 100, 200 and 400 Krad was applied to the grain either soon after moisture equilibration (3 days after inoculation) or 10 days later (13 days after inoculation). And the results were as in the followings. 1. Increase in moisture content from 17% to 25% greatly increased the aflatoxin concentration, especially at zero irradiation dose. 2. Prolongation of the incubation period prior to irradiation from 3 to 13 days resulted in greater accumulation of aflatoxin. 3. Two hundreds Krad applied 13 days after inoculation on barley stored at 25% moisture (100% RH) and $25^{\circ}C$ led to higher aflatoxin production than 100 Krad or even 50 Krad. 4. The relative proportion of the principal aflatoxins in relation to irradiation showed that aflatoxin G was elaborated at a significantly higher rate than aflatoxin B.

• The Korean Journal of Mycology
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• v.36 no.2
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• pp.144-147
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• 2008

Inonotus obliquus could be isolated from Betula platyphylla var. japonica with diameter in the range of 6$\sim$13 cm that artificially inoculated by the fungus. The diameter and/or inoculation point of tree did not show any significant relationships with the infection rate of the fungus. Inonotus obliquus showed rapid growth on vertical direction of the infected tree while the growth was quite low on radial direction. The isolated fungus from the infected tree did not show vegetative incompatibility with the original fungus used for inoculation. We could isolate 8 contaminants from the inoculated area; Trichoderma reesei, T. atroviride, Cryptococcus neoformans, Botrytis cinerea, Fusarium sp. and 3 unknown species.

• Korean journal of applied entomology
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• v.31 no.4
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• pp.551-559
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• 1992

These studies were conducted to investigate the biological effects of buprofezin 25% WP on the adult of the brown planthopper (BPH) and its control effects on the BPH population, when buprofezin was treated on different days after the introduction of the BPH adult to the potted rice. Also age structures of the BPH population at the time of buprofezin treatment were observed. The results were as follows: Buprofezin (7.0 g a.i./l0 a) could reduce the longevity and fecundity of the newly emerged female but did not affect the biology of older female, and it did not affect the biology of the male. The larval densities at the time of buprofezin treatment was reduced to nearly zero after treatment. The higher the residual effect and the proportion of larvae at the time of buprofezin treatment were, the higher its con¬trol effect on the BPH population was. In case of buprofezin 7.0 g a.i./l0a treatment, the time interval of treatment that could show the highest control effect was about 10 days (from 18 days after the adult introduction to the adult emergence days of next generation).

• Research in Plant Disease
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• v.7 no.3
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• pp.155-163
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• 2001

Two attenuated Cucumber mosaic virus (CMV) isolates, Paf-CMV and Rs2-CMV that had been selected from CMV isolates associated with satellite RNA (satRNA) were tested for cross-protection effect in pepper plants. The viruses selected as attenuated strains appeared to be identical serologically and physically to the challenge virus (Mf-CMV), but they were lower in the dilution end-point of infectivity of crude sap than Mf-CMV When symptoms were observed in several indicator plants after inoculation, Paf-CMV and Rs2-CMV were symptomless or showed mild mosaic symptoms while another satRNA isolate Ap-CMV developed severe mosaic symptoms on the leaves as Mf-CMV. The nucleotide sequences of the satRNAs were determined by sequencing full-length cDNA clones. Paf-, Rs2- and Ap-satRNAs were 386, 335, and 347 nucleotides long, respectively, The sequences were then compared with the other known Y-satRNA, revealing that nucleotide sequences of the satRNAs consisted of 5'- and 3'-terminal conserved regions. However variations occurred on the middle regions of the sequences, especially those related to symptom interference, showing significant differences between Paf-satRNA and other isolates. Infectious transcripts of Paf-satRNA and Rs2-satRNA induced mild mosaic symptoms in pepper plants when supported by genomic RNAs of Mf-CMV. Under greenhouse conditions, Paf-CMV and Rs2-CMV were tested for cross-protection effect in pepper and tobacco (Nicotiana tabacum cv, Xanthi nc) plants against Mf-CMV. No symptoms were developed on the plants vaccinated with Paf-CMV until 3 weeks after inoculation with the virulent strain; however another attenuated isolate, Rs2-CMV, showed less effectiveness in cross-protection. Depending on the concentration of the challenged virus, symptoms sometimes appeared later in the upper leaves. However, in plants challenged with low concentrations (below 0.2 mg/ml) of the challenge inoculum, symptoms caused by the virulent strain did not develop on the plants vaccinated with Paf-CMV. In the field experiments, the number of pepper plants with severe mosaic symptoms in the control plots was progressively increased after transplanting and reached approximately 50% after 50 days. On the other hand, the incidence of mosaic disease appeared very low on the plants that had received the protective inoculation with Paf-CMV.

• Proceedings of the Korean Society for Agricultural Machinery Conference
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• 2002.02a
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• pp.457-463
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• 2002

이 연구는 x-선 투과영상을 이용하여 홍삼의 내공을 검출하기 위하여 수행되었다. 이를 위하여 x-선관에 조사되는 x-ray 양의 차이에 따른 위치별 밝기 값 차이를 보정하고 내공 검출에 적합한 영상처리 알고리즘을 개발하였다. 주요연구 결과를 요약하면 다음과 같다. 1) x-선관에 조사되는 x-ray양은 중심에서 가장자리로 갈수록 감소하며, 이것은 위치에 따른 gray 값의 분포가 달라지게 되는 원인이 되었다. 2) 홍삼을 2치화 하기 위해서는 동일한 x-선 조사강도에서 입력된 원 영상에서 빈 영상을 뺀 감산 영상을 만들어 줌으로써 가능하였다. 3) 조사강도 별 감산영상처리 결과 36kV/4.15mA와 39kV/4.15mA에서는 조사량이 많아서 홈삼 지근 부분의 영상이 손실되는 경우가 발생하였다. 4) 내공 검출을 위해서는 정상부분과 내공부분의 자기 값 차이를 크게 하는 전처리 영상을 만들 필요가 있었고, multiple 감산영상에서 뚜렷한 골짜기가 나타났으나 주근의 내공부분의 밝기값 보다 지근의 정상부분의 자기 값이 더 낮게 나타나 이를 보정하기 위하여 홍삼 위치에 따라 부분 보정된 알고리즘을 개발하였다. 5) x-선관의 놓인 위치에 따라서 내공판정시험 결과 중심에 있을 때에 비해 가장자리에 있을 때는 영상이 일부 손실되었으며, 중심 위치에서 서로 다른 굵기의 홍삼에 대해서는 모두 양호한 결과가 나타났다. 6) 완전한 홈삼으로 내공판정시험 결과 내공주위의 정상부분가지도 일부 내공으로 잘못 검출되었으나 이것을 재차 line profile에 의해 한 라인씩 문턱 값을 설정하여 내공만을 정확하게 판정하는 알고리즘을 개발하였다.양체의 접종작업은 모든 배양실이 인력에 의존하였으며, 배양체를 배지와 분리하여 불필요한 부분을 제거하고 배양작물에 따라 생육정도를 2~3등급으로 구분하여 배양용기의 배지 위에 치상하는 과정으로 수행되었으며, 작업능률은 호접란의 경우 배양병에 25본을 접종하는데 시간당 6병, 심비디움은 원형 플라스크에 25본을 접종하는데 시간당 10병 정도였다. 바. 식물체의 대량증식에 사용되는 플라스크, 배양병, PE용기 등 배양용기의 세척작업은 농원의 1개배양실에서 간이식 세척기, 이 외의 9개배양실은 모두 물에 담겨 두었다가 세제와 브러쉬 등을 사용하여 인력으로 세척하고 있어 생력화 기술개발이 요구되었다.도가 빠를수록 건조속도가 빨라졌으며, 건조에너지도 1,334kcal/kg.water로 비슷하게 소요되었다. 마. 시험구와 대비구의 건감률은 시험구에서 1.08~1.36w.b./h로 나타나 대비구보다 약 9.9~18.3%가 높게 나타났고, 건조에너지는 10.2~14.6%가 절감되었다. 발아율은 열풍온도가 낮을수록 높게 나타났고 시험구가 대비구보다 발아율이 낮게 나타났으며, 동할률 증가량도 원적외선.열풍 복합건조방법이 높게 나타나 이것은 곡물 표면에 원적외선 방사에의한 복사열이 전달되어 열장해를 받았기 때문으로 판단되며, 금후 더 연구하여 적정 열풍온도 및 방사체 크기를 구명해야 할 것이다.으로 보여진다 따라서 옻나무 유래 F는 포유동물의 생식기능에 중요하게 작용하는 것으로 사료된다.된다.정량 분석한 결과이다. 시편의 조성은 33.6 at% U, 66.4 at% O의 결과를 얻었다. 산화물 핵연료의 표면 관찰 및 정량 분석 시험시 시편 표면을 전도성