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http://dx.doi.org/10.3746/jkfn.2014.43.5.756

Quality Characteristics of Fermented Soybean Products by Bacillus sp. Isolated from Traditional Soybean Paste  

Lee, Sun Young (Dept. of Agrofood Resources, NAAS, RDA)
Eom, Jeong Seon (Dept. of Agrofood Resources, NAAS, RDA)
Choi, Hye Sun (Dept. of Agrofood Resources, NAAS, RDA)
Publication Information
Journal of the Korean Society of Food Science and Nutrition / v.43, no.5, 2014 , pp. 756-762 More about this Journal
Abstract
This study evaluated the quality characteristics of soybean fermented with several Bacillus sp., which were selected based on their high enzymatic and antimicrobial activities. Total aerobic bacterial counts of fermented soybeans with HJ5-2 ($3.00{\times}10^9CFU/mL$) were the highest among all strains. Lactic acid bacteria numbered $2.50{\times}10^2{\sim}7.30{\times}10^4CFU/mL$ in soybeans fermented with isolates. Amylase and protease activities of the RD7-7 sample were the highest among all strains. Reducing sugar and amino-type nitrogen contents of fermented soybeans with HJ18-4 (2.35%) and RD7-7 (227.96 mg%) were the highest. Total amino acid contents of the samples were 16.62~18.38%, and glutamic acid, aspartic acid, leucine, lysine, arginine were major amino acids. Oxalic acid (36.51~63.57 mg/100 g) and succinic acid (429.49~600.15 mg/100 g) were the predominant organic acid. These results provide useful information for development starter (single and complex) as well as for the production of high quality fermented soybean foods.
Keywords
fermented soybean products; Bacillus sp.; quality characteristics;
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Times Cited By KSCI : 18  (Citation Analysis)
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1 Lee SY, Park NY, Kim JY, Choi HS. 2012. Quality characteristics of rice-doenjang during fermentation by differently shaped meju and adding starter. Korean J Food & Nutr 25: 505-512.   과학기술학회마을   DOI   ScienceOn
2 Suk AR. 2006. Quality of chungkookjang with wild microorganisms. MS Thesis. Kyungpook National University, Daegu, Korea.
3 Park JS, Lee MY, Lee TS. 1995. Compositions of sugars and fatty acids in soybean paste (doenjang) prepared with different microbial sources. J Korean Soc Food Nutr 24: 917-924.   과학기술학회마을
4 Hong SW, Kim JY, Lee BK, Chung KS. 2006. The bacterial biological response modifier enriched cheongkukjang fermentation. Korean J Food Sci Technol 38: 548-553.   과학기술학회마을
5 Baek LM, Park LY, Park KS, Lee SH. 2008. Effect of starter cultures on the fermentative characteristics of cheonggukjang. Korean J Food Sci Technol 40: 400-405.   과학기술학회마을
6 Rozan P, Kuo YH, Lambein F. 2000. Free amino acids presentin commercially available seedlings sold for human consumption. A potential hazard for consumers. J Agric Food Chem 48: 716-723.   DOI   ScienceOn
7 Park JH, Han CK, Choi SH, Lee BH, Lee HJ, Kim SS. 2011. Development of odor-reduced Korean traditional cheonggukjang added with job's tears. J Korean Soc Food Sci Nutr 40: 259-266.   과학기술학회마을   DOI   ScienceOn
8 Lee DG, Kim NY, Jang MK, Yoo BH, Kim KY, Kim SG, Jeong YK, Lee SH. 2006. Isolation of a fibrinolytic bacterium from Cheongkukjang and characterization of its bioactivity. Kor J Microbiol Biotechnol 34: 299-305.   과학기술학회마을
9 Hwang HA, Lee NK, Cho IJ, Hahm YT, Kwon KO, Kim BY. 2008. Selection of microorganisms and optimization of manufacture process for cheonggukjang. Korean J Food Sci Technol 40: 406-411.   과학기술학회마을
10 Kim IJ, Kim HK, Chung JH, Jeong YK, Ryu CH. 2002. Study of functional chungkukjang contain fibrinolytic enzyme. Korean J Life Sci 12: 357-362.   과학기술학회마을   DOI
11 Zheng YF, Jeong JK, Choi HS, Park KY. 2011. Increased quality characteristics and physiological effects of chunggukjang fermented with Bacillus subtilis-SKm. J Korean Soc Food Sci Nutr 40: 1694-1699.   과학기술학회마을   DOI   ScienceOn
12 Lee JJ, Cho CH, Kim JY, Lee DS, Kim HB. 2001. Antioxidant activity of substances extracted by alcohol from Chungkookjang powder. Kor J Microbiol 37: 177-181.   과학기술학회마을
13 Son DH, Kwon OJ, Ji WD, Choi UK, Kwon OJ, Lee EJ, Cho YJ, Cha WS, Chung YG. 2000. The quality changes of cheonggukjang prepared with Bacillus sp. CS-17 during fermentation time. J Korean Soc Agric Chem Biotechnol 43: 1-6.   과학기술학회마을
14 Allagheny N, Obanu ZA, Campbell-Platt G, Owens JD. 1996. Control of ammonia formation during Bacillus subtilis fermentation of legumes. Int J Food Microbiol 29: 321-333.   DOI   ScienceOn
15 Kim YS, Jung HJ, Park YS, Yu TS. 2003. Characteristics of flavor and functionality of Bacillus subtilis K-20 chunggukjang. Korean J Food Sci Technol 35: 475-478.   과학기술학회마을
16 Youn KC, Kim DH, Kim JO, Park BJ, Yook HS, Cho JM, Byun MW. 2002. Quality characteristics of the chungkookjang fermented by the mixed culture of Bacillus natto and B. lichniformis. J Korean Soc Food Sci Nutr 31: 204-210.   과학기술학회마을   DOI
17 Kwon HY, Kim YS, Kwon GS, Kwon CS, Sohn HY. 2004. Isolation of immuno-stimulating strain Bacillus pumilus JB-1 from Chungkook-jang and fermentational characteristics of JB-1. Kor J Microbiol Biotechnol 32: 291-296.   과학기술학회마을
18 Yoon KH. 2012. Properties of ${\beta}$-galactosidase from Bacillus licheniformis isolated from Cheongkookjang. Korean J Microbiol Biotechnol 40: 17-22.   과학기술학회마을   DOI
19 Yoon KH, Shin HY. 2010. Medium optimization for the protease production by Bacillus licheniformis isolated from Cheongkookjang. Kor J Mocrobiol Biotechnol 38: 385-390.   과학기술학회마을
20 Kim SS, Lee JH, Ahn YS, Kim JH, Kang DK. 2003. A fibrinolytic enzyme from Bacillus amyloliquefaciens D4-7 isolated from Chungkook-Jang: its characterization and influence of additives on thermostability. Kor J Microbiol Biotechnol 31: 271-276.   과학기술학회마을
21 Woo SM, Kwon SH, Jeong YJ. 2006. Selection and fermentation characteristics of cheongkukjang stains. Korean J Food Preserv 13: 77-82.   과학기술학회마을
22 Choi UK, Son DH, Ji WD, Im MH, Choi JD, Chung YG. 1998. Changes of taste components and palatability during chungkukjang fermentation by Bacilllus subtillis DC-2. J Koream Soc Food Sci Nutr 27: 840-845.   과학기술학회마을
23 Kim HJ, Lee JJ, Cheigh MJ, Choi SY. 1998. Amylase, protease, peroxidase and ascorbic acid oxidase activity of kimchi ingredients. Korean J Food Sci Technol 30: 1333-1338.   과학기술학회마을
24 Yoon KS. 1988. Changes of enzymatic activities during the fermentation of soybean-soy paste by Aspergillus spp. MS Thesis. Kunkuk University, Seoul, Korea.
25 Lee JO, Ha SD, Kim AJ, Yuh CS, Bang IS, Park SH. 2005. Industrial application and physiological functions of cheonggukjang. Food Sci Ind 38: 69-78.
26 Ashiuchi M, Kamei T, Baek DH, Shin SY, Sung MH, Soda K, Yagi T, Misono H. 2001. Isolation of Bacillus subtilis (chungkookjang), a poly-gamma-glutamate producer with high genetic competence. Appl Microbiol Biotechnol 57: 764-769.   DOI   ScienceOn
27 Kim JS. 1996. Current research trends on bioactive function of soybean. Korean Soybean Digest 13(2): 17-24.
28 Eom SM, Jung BY, OH HI. 2009. Changes in chemical components of cheonggukjang prepared with germinated soybeans during fermentation. J Appl Biol Chem 52: 133-141.   과학기술학회마을   DOI
29 Cho YJ, Cha WS, Chun SS, Choi UK, Bok SK, Kim MU. 2000. Production and separation of anti-hypertensive peptide during chunggugjang fermentation with Bacillus subtilis CH-1023. J Korean Soc Agric Chem Biotechnol 43: 247-252.   과학기술학회마을
30 Lee NR, Go TH, Lee SM, Hong CO, Park KM, Park GT, Hwang DY, Son HJ. 2013. Characteristics of chungkookjang prepared by Bacillus amyloliquefaciens with different soybeans and fermentation temperatures. Kor J Microbiol 49: 71-77.   과학기술학회마을   DOI
31 Choi HS, Joo SJ, Song IG, Min KB, Kim KS, Yoon HS. 2007. Quality characteristic of hwangki (Astragalus membranaceus) chungkukjang during fermentation. Korean J Food Preserv 14: 356-363.   과학기술학회마을
32 Miller GL, Blum R, Glennon WE, Burton AL. 1960. Measurement of carboxymethylcellulase activity. Anal Biochem 2: 127-132.