• Proceedings of the Korean Institute of Building Construction Conference
• /
• 2023.05a
• /
• pp.199-200
• /
• 2023

This paper presents a study on the selection of optimal mix proportions for producing lightweight pre-foam concrete as a substitute for Autoclaved Lightweight Concrete (ALC) without the accelerated curing. The study was conducted using a rapid hardening binder made from by-products of the steel industry as the primary raw material. The experimental results established the optimal mix proportions, which included retarder content, water/binder ratio, foam content, and fiber inclusion amount, for the production of lightweight foam concrete. The optimal mix proportion was determined to have a retarder content at the minimum amount required to secure the working time, W/B of 35%, a foam content limited to 65% or less, and a fiber inclusion amount of 0.05% or less.

• Proceedings of the Korean Society of Embryo Transfer Conference
• /
• 2002.11a
• /
• pp.79-79
• /
• 2002

본 연구에서는 in vitro에서 성숙된 난자의 핵성숙(Polar Body extrusion)에 소요되는 시간과 배반포 단계로의 발달능력 사이의 관계를 비교하여 조기에 발달능력을 가진 embryo를 선발할 수 있는 IVP 체계를 개발하고자 하였으며 in vitro maturation(IVM)에 따른 first polar body(PB) 형성, IVM과 IVF 시간이 oocyte의 발달에 미치는 영향과 생산된 배반포의 세포수를 평가하였다. IVM은 TCM199 배양액을 사용하였고 in vitro fertilization(IVF)은 Fer -TALP용액을 사용하였으며 in vitro culture(IVC)는 CRlaa 배양액을 사용하여 2일까지는 0.3% BSA를 3일 부터는 10%FBS와 bovine oviduct epithelial cell을 첨가하여 배양하였다. IVM 시간에 따른 PB의 출현율은 0hr(0%), 6hr(0%), 12hr(0%), 14hr(8.7%), 16hr(40.5%), 18hr(48.0%), 20hr(65%), 22(68%) 그리고 24hr(74.5%)을 보였으며 IVM 시간에 따른 cleavage 및 8cell 발달율 사이에는 유의적인 차이가 없었으나 배반포(BL) 및 8cell에서 배반포로 발달률은 18시간(BL 31$\pm$6, BL/8cell 82 $\pm$5%)에서 가장 높게 나타났으며 24시간(BL 17$\pm$2, BL/8cell 60$\pm$8%)과 유의적인 차이를 보였다(P<0.05). IVC 7일째 배반포의 총세포수와 trophoblast(TE) 세포수는 IVM 18시간(mean$\pm$S.E.; total: 131.1$\pm$34.0, TE: 97.6$\pm$29.6)에서 24시간(total: 112.2$\pm$17.5, TE: 80.1$\pm$15.6)보다 유의하게 많은 것으로 나왔으나(P<0.05) 7일째의 inner cell mass(ICM) 숫자(18hr 33.5$\pm$12.8 vs 24hr 32.1$\pm$12.0)와 8일째 ICM, TE 그리고 총 세포수에는 유의성 있는 차이가 없었다. IVM 18시간에서 PB 형성과 8cell 발달률 사이에 높은 상관성을 보였고 배반포 및 8cell에서 배반포 단계로 높은 발달률을 보였으며 생산된 배반포의 TE 숫자와 총 세포수가 유의하게 많은 것으로 나타났다. 따라서 IVM 18시간 실시하였을 경우 보다 많은 세포수를 가진 배반포 발달 가능성이 높은 embryo를 조기에 선발 가능할 것으로 사료된다.

• Korean Journal of Medicinal Crop Science
• /
• v.16 no.2
• /
• pp.118-123
• /
• 2008

This study was executed to breed new cultivar of E. senticosus suitable for seed harvesting by selection method from 896 native plants collected from 35 regions. Basic selection points were C.V. values of characters such as filament length, blooming period, shoot length, no. of shoot and hundred fruits weight. By four steps of selection, two lines having high biomass yielding, diseases tolerant and fruiting capacities were finally selected and registered as new cultivar of Eleutherococcus senticosus at the Korea seed & variety service by regulation for seed production and marketing of plant variety.

• Korean Journal of Breeding Science
• /
• v.41 no.1
• /
• pp.32-35
• /
• 2009

A new poinsettia cultivar "Early bird" was bred by the National Institute of Horticultural and Herbal Science (NIHHS) in 2007. A cross was made between 'Ichiban', a variety with deep lobed transitional leaves and bright red bracts, and 'Freedom Red', a vigorously growing dark red colored variety in 2004. "Early bird" was finally selected in 2007 after the investigation of the growth and flower characteristics from 2005 to 2007. "Early bird" has bright red colored, wide obovate bracts. Leafblade is dark green and ovate. Plant height is short and stem color is greenish. Response to short day is early and its bracts and transitional leaves are fully colored 6.5 weeks after short day commencement.

• Journal of the Korean Data and Information Science Society
• /
• v.24 no.4
• /
• pp.793-802
• /
• 2013

There are many problems to single sports talents out of test applicants. The physical activity promotion system has been performed to all elements school students of both 5 and 6 grades in order to evaluate overall health and physical activities since 2009. This system includes some variables which could measure the students' sports latent power, so that the system could be used to single out sports talents. In this work, we propose a primary screening method that element school teachers might evaluate sports talents based on the data of the physical activity promotion system. Two more sports talent indices which are sports flexibility index and sports endurance index are defined. The selection method of sports talents is developed by using sports latent indices including sports power and cardiorespiratory indices. This method is efficient from the view of time and cost aspects, since we do not need to remeasure all elements school students again.

• Korean Journal of Medicinal Crop Science
• /
• v.15 no.3
• /
• pp.177-182
• /
• 2007

This study was conducted to produce high quality ginseng seedlings by improvement of the physical properties of nursery soil. In order to select optimum nursery conditions, nursery types were treated with the yangiik nursery and semi-yangiik nursery. Soil conditioners were treated with two different materials of puffed rice husks and perlite. This experiments were tested at Gyunggido Agricultural Research and Extention Services, Hwaseng, from 2003 to 2004. Soil permeability was better at the plot of yangiik nursery than that of semi-yangjik nursery, and was better at plot of puffed rice husks than that of perlite. Soil porosity was improved 2.2% at plot of yangjiik nursery compare at that of semi-yangjik nursery, and was improved 2.0% at plot of puffed rice husks compared to control. There was no difference in the number of ginseng plants per kan among treatments. Root length was longer at plot of yangjik nursery than that of semi-yangjik nursery. More available ginseng plants per kan were obtained from the plot of yangjik nursery by more 252 seedlings than that of semi-yangjik nursery, but there was no significant difference between soil conditioners. The number of available seedlings per kan produced from yangjik nursery was 21.7% higher than those produced by semi-yangjik nursery. The number of available seedlings per kan produced from plot of puffed rice husks was 16.3% higher than that of control.

• The Korean Journal of Mycology
• /
• v.38 no.1
• /
• pp.48-53
• /
• 2010

Agrobacterium-mediated transformation was conducted in order to generate DNA insertional mutants of Flammulina velutipes. Agrobacterium tumefaciens AGL-1 harboring pBGgHg was transformed into gill tissues of Flammulina velutipes strain KACC42777. The transformants resistant on hygromycine ($30\;{\mu}g/ml$) were confirmed by PCR. The targeted insertional sites were amplified by inverse PCR and sequenced. To find the phenotype variation of all generated transformants, bottle cultivation which followed by the standard cultivation protocol were conducted. Color variation was observed on the cultivated fruiting bodies. Furthermore, the transformant pool will be used as a good genetic resources for studying gene function.

• Environmental Analysis Health and Toxicology
• /
• v.24 no.4
• /
• pp.321-332
• /
• 2009

본 연구의 목적은 다이아지논(Diazinon; O, O-diethyl O-[6-methyl-2 (1-methylethyl)-4-pyrimidinyl] phosphorothioate)에 노출된 모델 생물체(송사리)의 행동변화와 관련된 분자생물학적 기전 규명을 통하여 비정상적 행동의 모니터링을 위한 생물지표(biomarker)를 개발하는데 있다. 이를 위해 우선 suppression subtractive hybridization (SSH) 및 DNA microarray 기법을 활용하여 다양한 유전자를 스크리닝하였다. 다이아지논에 노출시킨 송사리에서 발현의 차이가 나는 상향 조절된 유전자 97개 (알려지지 않은 유전자 27개 포함)와 하향 조절된 유전자 99개 (알려지지 않은 유전자 60개 포함)를 동정 하였고 이들 중 이상행동과 관련되는 것으로 보이는 유전자 10개 (상향조절 5개, 하향조절 5개)를 선발하였다. 이들 중에서 primer 제작이 잘된 beta-1, Orla C3-1, parvalbumin 및 apolipoprotein E을 선발하여 그 유전자 발현을 real-time PCR 기법을 사용하여 정량적으로 모니터링 하였다. Orla C3-1, parvalbumin 및 apolipoprotein E는 고농도의 다이아지논 처리(1000 ppb; 24 h)에서 그 발현이 억제됨이 관찰되었다. 다이아지논 처리 시 신경질환 (알츠하이머 병 및 다운신드롬)에 관련된 apolipoprotein E와 근육세포의 유연화에 작용하는 parvalbumin 등의 발현억제는 송사리의 인지능력 교란 및 근육세포의 경직 등을 각각 유도하여 송사리의 비정상적 행동을 야기하는 것으로 판단되었다. 따라서 이들 생물지표는 신경독성물질에 의한 송사리 및 기타 어류의 이상행동의 변화의 감지에 활용될 수 있을 것으로 사료된다.

• Korean Journal of Breeding Science
• /
• v.42 no.6
• /
• pp.664-667
• /
• 2010

A new poinsettia cultivar 'Clara' was bred by the National Institute of Horticultural and Herbal Science (NIHHS) in 2005. A cross was made between 'Red Velvet', a variety with dark green leaves and red bracts, and 'Freedom Bright Red', a variety with early short day responding and bright red bracts, in 2002. 'Clara' was finally selected in 2005 after the investigation of the growth and flowering characteristics from 2003 to 2005. 'Clara' has red ovate bracts and weak intensity of rugosity between bract veins. Leaf blade is ovate and medium green. Petiole length is relatively short, and stem color is reddish. 'Clara' has medium free-branching ability and makes short and compact canopy. Clara's bracts and transitional leaves are fully colored 9 weeks after short day commencement.

• Korean Journal of Breeding Science
• /
• v.42 no.6
• /
• pp.611-614
• /
• 2010

A new poinsettia cultivar 'Heidi' was bred by the National Institute of Horticultural and Herbal Science (NIHHS) in 2004. A cross was made between 'Freedom Rose', a variety with vigorously growing deep pink color, and 'Red Velvet', a variety with dark green leaves and red bracts in 2001. 'Heidi' was finally selected in 2004 after the investigation of the growth and flower characteristics from 2002 to 2004. It has velvety red colored elliptic bracts and transitional leaves, and medium intensity of rugosity between bract veins. Leaf blade is dark green, ovate shape and lobes are shallow. Petiole length is relatively short and stem color is reddish. 'Heidi' has free-branching ability and plant height is short. The bracts and transitional leaves are fully colored 7.5 weeks after short day commencement.