• Journal of the Korean Recycled Construction Resources Institute
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• v.4 no.3
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• pp.316-321
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• 2016

This study developed an eco-block for grass growth using the expanded vermiculites that absorb bacteria selected considering for the high pH and dry environments and plant growth. For the developed eco-block, a fundamental properties including compressive strength gain and water absorption and ecology characteristics were tested. The selected bacteria was Bacillus alcalophilus a nd Rhodoblastus acidophilus and had high concentration of $10^9cell/mL$. The expanded vermiculite that was used for shelter of bacteria was added by 7.5% and 10% replacement of the natural aggregates by volume. The developed eco-block achieved the minimum requirements specified in SPS provision and significantly effective in reducing chemical Chemical Oxygen Demand(COD) concentration and enhancing the growth of fish and plant.

• Proceedings of the Korea Water Resources Association Conference
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• 2017.05a
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• pp.552-552
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• 2017

본래 식용으로 들여온 외래어종은 우리나라 하천에서 토착 어류의 생태계를 교란시키며, 빠르게 서식처를 확산하고 있다. 일반적으로 알려져 있는 외래어종인 배스와 블루길 이외에도 무지개 송어 및 향어도 외래어종으로 분류된다. 본 연구는 외래어종 중 배스와 블루길을 대상으로 여러 환경적 요인에 따른 서식처 형성을 관찰하고 외래어종의 생태활동 변화를 분석하는 것을 목적으로 수행하였다. 여러 연구에 따르면 수온과 용존산소가 담수 어류의 서식환경 구축을 결정하는 매우 중요한 인자임을 알 수 있다. 이에 따라, 외래어종의 서식처 형성에 영향을 미치는 여러 환경적 요인 중 수온을 주요 인자로 선정하여 실험실 실험을 수행하였다. 연구를 수행하기 위해 하천 및 농업용 저수지로부터 배스와 블루길을 채집하였다. 채집된 외래어종을 길이 12 m, 깊이 0.6 m, 폭 1 m의 하천과 유사한 자연적 환경이 갖추어진 수조에 이식하였다. 수조 내 환경은 독립변인인 수온을 제외하고 모두 동일하게 구성하였다. 수조를 3개의 구역으로 구분하여 양 끝단에 냉수대와 온수대를 조성하였으며, 중간부의 평균 수온대를 기준으로 냉수대와 온수대의 수온 차는 약 $1^{\circ}C$이상 유지되도록 설정하였다. 현재까지의 실험 결과에 따르면, 외래어종은 온수대에 서식처를 형성하였다. $1^{\circ}C$의 수온 차에도 매우 민감하게 반응하며, 냉수대에는 서식처를 형성하지 않는 것 또한 확인되었다. 이를 통해 수온이 외래어종의 서식처 형성에 매우 중요한 영향을 미치는 요인인 것으로 판단된다. 연구 과정에서 추가적으로, 먹이 금붕어가 부재할 경우 동종 어식을 하는 것이 관찰되었다. 수조 내 수온에 따른 구역의 구분을 위해 공통으로 설치된 가림판 부근에 외래어종의 군락이 형성된 것을 통해, 향후에는 외래어종의 서식처 형성과 장애물과 같은 지형적인 요인이 미치는 영향에 관한 연구가 필요할 것으로 판단된다. 춘계는 수온의 증가로 외래어종이 더욱 왕성한 활동을 하는 시기이다. 수온이 외래어종의 서식처 형성에 지배적인 영향을 미치는 것이 실험을 통해 확인하였으나, 수온에 대한 민감도에 관한 연구 또한 필요하다. 더불어 외래어종의 계절에 따른 먹이활동 및 생장량 또한 지속적으로 관찰할 것이다.

• Proceedings of the Korean Society of Fisheries Technology Conference
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• 2001.05a
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• pp.495-496
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• 2001

저서 해조류의 수직분포에 대한 광의 영향은 주로 유효 광에 대한 종간의 상이한 반응의 결과에 의한다. 특히 조하대의 Kelp군집의 형성은 배우체나 어린 포자체와 같은 부착단계에 있어서의 서식처의 광조건에 의해 중요하게 영향을 받는다(Luning, 1981; Drew, 1983). 이것은 광이 이들의 광합성의 에너지원으로써, 광량, 광질, 광주기의 변화가 조류의 생장을 시ㆍ공간적으로 제한하고 있기 때문이다. (중략)

• Journal of the Korean Recycled Construction Resources Institute
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• v.4 no.2
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• pp.165-171
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• 2016

The objective of this study is to evaluate the compressive strength development and ecological characteristics of mortars using expanded vermiculite absorbing bacteria as a fundamental investigation to develop precast eco-concrete products. For bacterial growth under the high-alkalinity and high-dried environments within hardened mortars and for creating plant growth function to mortars, Bacillus alcalophilus and Rhodoblastus acidophilus were separated and cultured. The cultured bacteria were absorbed into expanded vermiculite selected for bacteria shelter. The expanded vermiculite absorbing bacteria was then added into mortar mixture as a volumetric replacement of fine aggregate. Test results showed that the developed technology is very effective in enhancing the plant growth onto the hardened mortars and reducing the COD and T-N concentration in raw water. The optimum replacement level of expanded vermiculite absorbing bacteria can be recommended to be less than 10% considering the compressive strength development and cost of mortars along with the ecological effectiveness.

• Journal of Life Science
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• v.9 no.1
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• pp.1-7
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• 1999

To isolate of antagonistic bacteria to Phytophthora capsici, which cause Phytophthora blight in red pepper, 237 isolates of Pseudomonas spp. and 260 isolates of Bacillus spp. were screened in selective media from rhizosphere soils of red pepper at Kyongsan, Kyongju, Yongchon and Euisung in Kyongbuk. Among total 497 isolates, 8 isolates of Pseudomonas spp and 4 isolates of Bacillus spp. inhibited the mycelial growth of Phytophthora capsici above 50$\%$ . These antagonistic bacteria showed more inhibitory effect on TSA (tryptic soy agar) than V-8 juice agar. Four isolates, P0704, P1201, B1101 and B1901, showing the most prominent antagonistic activity were selected and identified as P. cepacia (P0704, P1201), B. polymyxa (B1101) and B. subtilis (B1901), respectively. Cell free filtrates of these isolates were shown to inhibit zoosporangia germination and mycelial growth of p. capsici indicating that these isolates turned out to be bacteria producing antifungal substances. As a result of antagonistic test to Phytophthora blight in green house p. cepacia (P0704) showed the highest antagonistic effect with 46.7$\%$ and the rest of them were in the range of 13.4$\%$ to 26.7$\%$ .

• The Korean Journal of Pesticide Science
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• v.18 no.3
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• pp.168-174
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• 2014

It was conducted to evaluate the control efficacy of Bordeaux mixture on pepper anthracnose in vitro and in vivo. The suppressive efficacy of Bordeaux mixture on mycelial growth and spore germination of Colletotrichum acutatum was investigated on PDA, cellophane membranes and pepper fruits, respectively. Furthermore, its control value was evaluated on detached pepper fruits inoculated with C. acutatum by wound and non-wound inoculation method, and in fields. The mycelial growth of C. acutatum JC24 was inhibited by 90.0% on PDA amended with $937{\mu}g\;mL^{-1}$ of Bordeaux mixture. While the spore germination of C. acutatum JC24 was inhibited perfectly on cellophane membranes treated at $187{\mu}g\;mL^{-1}$ of Bordeaux mixture, that on fruits inoculated with the pathogen by wound inoculation and non-wound inoculation method was inhibited by 88.1% and 91.3%, respectively. Although the control value on fruits treated with $937{\mu}g\;mL^{-1}$ of Bordeaux mixture was 17.6% in wound inoculation method, it was 58.8% in non-wound inoculation method. In fields, when Bordeaux mixture was sprayed five times at 14 day-intervals, it showed 55.7% and 61.7% of control value in 2012 and 2013, respectively. We think Bordeaux mixture was able to use as an eco-friendly organic farming material to control pepper anthracnose based on the above-mentioned results.

• Journal of the Korean Institute of Landscape Architecture
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• v.28 no.3
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• pp.1-12
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• 2000

식물생장조절제(Plant Growth Regulators, PGRs)를 이용한 예초관리 방법이 들잔디 엽색 및 품 질에 미치는 영향을 규명함으로 잔디 적응력 관점에서 적합한 PGR 종류 및 살포수준을 파 악하고자 본 연구를 시작하였다. 실험은 들잔디 생육이 왕성한 시기인 6월(실험 I)과 7월(실험 II) 2회에 걸쳐 각각 다른 장소에서 실시하였다. 공시약제 처리구는 Type I PGR인 amidochlor 3 수준 및 mefluidide 3 수준 과 Type II PGR인 trinexapacethyl 4 수준, 그리고 무처리구를 포함하여 전체 11 처리구였 고, 실험구 배 치는 난괴법 4 반복으로 하였다. 공시한 3종류 PGR 모두 잔디엽색 및 품질에 통계적으로 유의한 차이 가 있었지만, PGR 종류.살포수준 및 생육 상태에 따라 처리효과는 다르게 나타났다. 잔디 엽색 및 품질 은 PGR 살포후 경과기간에 따라 점진적으로 억제되는 경향이었다. 처리 1주후 잔디품질은 대조구에 비 해 큰 차이가 없었지만, 2주에서 4주까지는 PGR 종류 및 살포수준에 따라 유의하게 감소하 여 0.08 ml/ $m^2$ 이상의 trinexapac-ethyl 처리구 및 1.20 ml/$m^2$ 이상의 mefluidide 처리구에서는 가장 크 게 나타났다. 처리 4주부터 대부분 처리구에서 잔디품질이 적정수준 이상으로 회복되기 시작하였다. 본 실험을 통해 잔디관리 수준에서 저관리 잔디지역은 0.08 ml/$m^2$ 수준의 trinexapac-ethyl 처리구가 장기간 생장억제 관점에서 실용성과 경제성 측면에서 들잔디 관리에 효율적인 것으로 판단되었고, 고관리 잔 디지역은 0.04~0.08ml/$m^2$ 수준의 trinexapac-ethyl 처리구 및 0.60~1.20 ml/$m^2$ 수준의 amidochlor 처 리구가 적합 한 것으로 판단되었지만, 계절에 따라 PGR 효과정도가 다르게 나타났다. 따라서, 잔디 관리 시 잔디의 생육상태, 예초 절감 기간, 잔디품질의 기대수준 및 적용할 수 있는 관리수준에 따라 적합한 PGR 종류 선정과 함께 살포시기 및 살포량을 효율적으로 결정하는 것이 필요한 것으로 사료되었다.

• Research in Plant Disease
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• v.20 no.2
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• pp.87-94
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• 2014

The fungus Cylindrocarpon destructans is the cause of root rot in many ginseng production areas in Korea. A total of 57 isolates of C. destructans were recovered from diseased roots in a survey of ginseng-growing fields from 2011-2012. Among these isolates, 37% were classified as highly virulent (causing lesions on unwounded mature roots) and 61% were weakly virulent(causing lesions only on previously wounded roots). Radial growth of highly and weakly virulent isolates on potato dextrose agar was highest at $20^{\circ}C$ and there was no growth at $35^{\circ}C$. Mycelial mass production was significantly (P = 0.05) lower at pH 7.0 compared with pH 5.0. To study the effects of pH (5.0 and 7.0) and wounding on disease development, ginseng roots were grown hydroponically in nutrient solution. Lesions were significantly larger (P < 0.01) at pH 5.0 compared with pH 7.0 and wounding enhanced disease by a highly virulent isolate at both pHs. In artificially infested soil, 2-yearold ginseng roots were most susceptible to Cylindrocarpon root rot among all root ages tested (1 to 4 years) when evaluated using a combined scale of disease incidence and severity. Root rot severity was significantly (P<0.05) enhanced by increasing the inoculum density from $3.5{\times}10^2cfu/g$ of soil to $2.0{\times}10^3cfu/g$ of soil.

• Korean Journal of Plant Resources
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• v.15 no.1
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• pp.18-25
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• 2002

These experiments were conducted to evaluate the variability of seed germination, and seedling growth of PEG priming barley. The average germination percentage(AGP) of PEG priming seed was higher than control, but those were low with extend the treatment period. The AGP of washing seed after PEG priming was higher than unwashed seed, and that of redried seed after priming was lower than control. The germination time of priming seed was short compare to control seed, and that was prolonged with extend the priming period, and that of washing seed after priming was shortening, and that of redried seed after priming was prolonging. The emergence percentage(EP) of priming seed was higher than control, and the emergence time was shortest in 50％ field moisture capacity soil. The seedling and root length of priming seed was shorter than control.

• Journal of Life Science
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• v.14 no.5
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• pp.778-787
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• 2004

Effect of the microbial product made of Bacillus stearothermophilus DL-3, which was isolated from the soil and identified in this study, and rice bran on microorganisms in soil and growth of lettuce (Red skirt lettuce) and Chinese cabbage (Ga rack new No.1 Chinese cabbage) was investigated. Total numbers of microorganisms in the pot with untreated soil, treated with standard amount of microbial product and treated with double amounts of microbial product for growth of lettuce after 6 weeks were 2.78${\times}$10$^{7}$ CFU/g, 2.72${\times}$10$^{8}$ CFU/g and 3.63${\times}$10$^{8}$ CFU/g. Total numbers of microorganisms in the soil without treatment of microbial product and treated with standard amount of microbial product were 2.06${\times}$10$^{8}$ CFU/g and 5.49${\times}$10$^{8}$ CFU/g. Total numbers of microorganisms in the pot with untreated soil, treated with standard amount of microbial product and treated with double amounts of microbial product for growth of Chinse cabbage after 6 weeks were 1.43${\times}$10$^{7}$ CFU/g, 3.42${\times}$10$^{8}$ CFU/g and 7.22${\times}$10$^{8}$ CFU/g. Total numbers of microorganisms in the soil without treatment of microbial product and treated with standard amount of microbial product were 5.75${\times}$10$^{8}$ CFU/g and 7.96${\times}$10$^{8}$ CFU/g. On basis of leaf length, leaf width, leaf number, wet weight and dry weight, the growth of lettuce and Chinese cabbage on the soil treated with microbial product was faster than that on the untreated soil. The treatment of microbial product in the soil resulted in the increase of useful microorganisms, which seemed to enhance the growth of lettuce and Chinese cabbage.