• Title/Summary/Keyword: 생장조절제

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Plant Regeneration via Organogenesis from Leaf and Stipule Segments of Strawberry (Fragaria ananassa Duch.) (딸기의 잎과 탁엽 절편체로부터 기관형성을 통한 식물체 재생)

  • 최준영;김현정;형남인
    • Korean Journal of Plant Tissue Culture
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    • v.25 no.5
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    • pp.347-351
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    • 1998
  • Plant regeneration via organogenesis from leaf and stipule explants of micropropagated shoots of strawberry (Fragaria $\times$ ananassa cv. Suhong) was achieved. Leaf and stipule explants were detached from shoot-tip cultured shoots and cultured on MS medium with various combinations of BA and NAA under light or dark condition. Shoot regeneration from leaf explant was observed after 3 weeks in culture and was good at the high ratio of BA and NAA among various combination treatments. The highest shoot regeneration frequency from leaf explants was obtained with 1.0 mg/L BA and 0.1 mg/L NAA, in which 31.1% shoot regeneration frequency(1.7 shoots per leaf explant) was yielded. In case of stipule explants, shoot regeneration was largely affected by plant growth regulators during incubation under dark condition for initial 4 weeks but not under continuous light condition. The combination treatment with 2.0 mg/L BA and 0.1 mg/L NAA showed the most excellent shoot regeneration from stipule explants, where 44.4% regeneration frequency(4.0 shoots per explants) obtained. Regenerated shoots were rooted on MS medium with 0.1 mg/L NAA after shoot elongation, and the plantlets regenerated were transferred to soil mixtures with vermiculite and perlite for acclimation.

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Micropropagation of Heloniopsis orientalis (Thunb.) C. Tanska in vitro (조직배양을 이용한 처녀치마[Heloniopsis orientalis (Thunb) C. Tanaka] 대량 증식)

  • 윤세영;이명선;임상철;신중두
    • Korean Journal of Plant Tissue Culture
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    • v.27 no.3
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    • pp.197-202
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    • 2000
  • The effect of cultural media and growth regulators on multiple plant regeneration from leaf explants of Heloniopsis orientalis (Thunb.) Tanaka was evaluated. The highest percentage of shoot and root formation were 20 and 30% on MS medium treated at 3.0 mg $l^{-1}$ of zeatin, respectively. Also 67 and 33% of high shoot formation appeared on 1/2 MS and 5 culture medium treated at zeatin 1.0 and 3.0 mg $l^{-1}$ respectively. With MS treated at 0.5 mg $l^{-1}$ of 2,4-D 1/2 MS and B5 culture media treated at each 1.0 and 3.0 mg $l^{-1}$ of zeatin the highest ratios of plant produced were 100, 280 and 310 % respectively relative to the other treatments. Generally, there was highest possibility for multiple propagation of Heloniopsis orientalis (Thunb.) C. Tanaka with B5 culture media supplemented 3.0 mg $l^{-1}$ of zeatin.

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Effect of Transportation Conditions and Plant Growth Regulator on the Maintenance of Freshness and Quality of Cut Chrysanthemum 'Baekma' (절화국화 '백마'의 수송조건 및 생장조절제 처리가 선도유지 및 품질에 미치는 효과)

  • Suh, Jeung Keun;Kim, Ji Hee;Kim, Kyoung Ok
    • FLOWER RESEARCH JOURNAL
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    • v.19 no.4
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    • pp.206-211
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    • 2011
  • This study was carried the effect of transportation conditions and plant growth regulator on the maintenance of freshness and quality of cut Chrysanthemum 'Baekma'. The quality of cut chrysanthemum 'Baekma' which were stored at $5^{\circ}C$ was better than those of stored at $20^{\circ}C$ and control. When cut mum 'Baekma' was transported stacking boxes with vertical condition after dipping in distilled water, flowering degree, flower size, days to flower appearing degree, and bent neck were delayed. Flowering degree was delayed by $10mg/L^{-1}$ uniconazole spraying, The days to bent neck was delayed by 1,000x daminozide spraying.

Effect of Triazole Growth Regulator Treatment on the Growth of Plug Seedling and Yield of Tomato (토마토 육묘 시 Triazole계 생장조절제 처리가 묘의 생육 및 수량에 미치는 영향)

  • Yun, Hyung-Kwon;Seo, Tae-Chul;Lee, Ji-Weon;Yang, Eun-Young
    • Journal of Bio-Environment Control
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    • v.16 no.3
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    • pp.205-209
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    • 2007
  • This experiment was conducted to investigate the growth of plug seedlings and yield of tomato affected by the concentration of triazole and the treatment time. The length and leaf area of tomato seed-lings were reduced by the application of triazole compared to control. Growth reduction of seedlings was greater by earlier application and higher concentration of triazole. The growth of tomato treated with hexaconazole were similar with control 4 weeks after sowing. The triazole treatment lowered the height of the first fruit set compared to control. However, triazole treatment did not affect the number of fruits and yield.

Effect of Plant Growth Regulators and Media on Regeneration of Sorghum bicolor (L.) Moench (바이오에너지용 수수 품종의 재분화율 증진을 위한 배지와 생장조절제 효과)

  • Goh, Eun-Jeong;Seong, Eun-Soo;Yoo, Ji-Hye;Kil, Hyun-Young;Lee, Jae-Geun;Hwang, In-Seong;Kim, Nam-Jun;Ghimire, Bimal Kumar;Kim, Myong-Jo;Lee, Ju-Kyung;Lim, Jung-Dae;Kim, Na-Young;Yu, Chang-Yeon
    • Korean Journal of Plant Resources
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    • v.24 no.2
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    • pp.168-173
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    • 2011
  • This study was carried out to optimize the embryogenic callus induction and plant regeneration from mature seeds of Sorghum bicolor. The effect of growth regulators was investigated on formation of embryogenic callus. The highest frequency of embryogenic callus was observed when the mature seeds were cultured on B5 medium supplemented with 2 mg/L 2,4-Dichlorophenoxyacetic acid (2,4-D). The highest frequency of plant regeneration from embryogenic callus was observed on MS medium with 0.5 $mg\;l^{-1}$ 6-benzyl amino purine (BAP) and 0.25 $mg\;l^{-1}$ indole-3-butyric acid (IBA) to optimize the shoot regeneration. High concentration of BAP (1 $mg\;l^{-1}$) supplemented with IBA (0.25 $mg\;l^{-1}$) was effective combination for shoot multiplication. MS medium supplemented with 1 $mg\;l^{-1}$ IBA was found to be the most effective for inducing roots. Normal rooted plantlets were transferred to the greenhouse for hardening with over 90% survival rate. Hence, this reproducible protocol could be useful for mass propagation and genetic transformation of S. bicolor.

Effect of Plant Growth Regulators on Lodging in Rice (식물(植物) 생장(生長) 조절제(調節劑)가 벼 도복(倒伏)에 미치는 영향(影響))

  • Lee, Sang-Chul;DeDatta, S.K.
    • Korean Journal of Weed Science
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    • v.11 no.2
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    • pp.87-99
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    • 1991
  • The experimental growth regulators Hoe78784 and PP-333 significantly reduced lodging in all rices. However, they did not significantly affect the crop vegetative characteristics and yield components, except by decreasing plant height when both growth regulators were applied at booting stage. Grain yield of IR21820-154-3-2-2-3 increased due to reduced lodging when Hoe78784 and PP-333 were applied at booting. These growth regulators increased the thickness and diameter of the culm. Applying CCC did not affect lodging in all test varieties. CCC-treated varieties and the control showed lower number of vascular bundles and culm thickness and diameter of the third and fourth internodes. Hoe78784 and PP-333 significantly reduced internode length from the second to the third internode in short-statured varieties. In tall varieties. reduction extended up to the fourth internode. Culm N content of the varieties did not significantly differ among treatments, except in IR8. Culm P content also did not differ significantly among treatments. although K and Si content was the highest than control when Hoe78784 was applied.

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Effects of Plant Growth Regulators on Growth of Rice Seedling for Machine Transplanting (식물생장조절제 처리가 벼기계이앙 어린모 생육에 미치는 영향)

  • 이주열
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.38 no.4
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    • pp.360-365
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    • 1993
  • The infant seedling of rice(8-to 10-day-old) has often suffered from submergence just after transplanting due to the too short seedling height, 5 to 8cm, This experiment was conducted to elucidate the effect of gibberellic acid($GA_3 : 25% soluble liquid) with the application methods of foliar spray and seed-soaking on the elongation of seedling height of infant rice seedling in machine transplanting, We used a japonica rice variety, Hwaseongbyeo, and the seeding rate was 200g per seed tray(30$\times60$\times3cm). Generally,$GA_3 and Metalaxyl increased the elongation of seedling height of infant seedling while Tetracycle decreased the seedling height, To increase the seedling height of infant seedling, the foliar spary application of $GA_3 was better than seed-soaking treatment. The optimum concentration of $GA_3 for the foliar spray was about l0ppm and the optimum application time of $GA_3 was 3 to 4 days after sowing.

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