• Title/Summary/Keyword: 생장상

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Effects of the Phytohormones on the Organ Differentiation and the Callus Induction from the Meristem Tip and the Segments of the Leaf and Stem of Potato by in vitro Culture (기내배양시 몇가지 생장조절물질이 감자의 생장점 및 경엽조직편으로부터의 Callus 및 기관분화에 미치는 영향)

  • 김충수;조재성;최창열
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.26 no.4
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    • pp.344-349
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    • 1981
  • This study was conducted to define the effect of 2.4-D, NAA, Benzyladenine, and basic mediums on the callus induction and the organ differentiation from the meristem tips and the stem and leaf segments of the potato. Benzyladenine promoted the induction and growth of shoot from the meristem tip of potato but inhibited initiation of roots and induction of callus. At higher concentration of NAA than 0.5 ppm and of 2.4-D than 1.0 ppm the shoots were not initiated but the callus was induced from the meristem. The callus growth was significantly promoted on the medium containing NAA than 2.4-0. The initiation and growth of the shoots from the potato meristem was significantly increased in the medium containing 2.4-D and BA, or NAA and BA, compared with those containing BA, NAA or 2.4-D alone. The callus was more easily induced from the stem segments than the leaf segments of potato. And the 2.4-D was more effective for the induction and growth of the callus than the NAA. MS medium diluted its concentration to 1/2 was more suitable for the initiation and growth of the shoots from the potato meristem than the MS standard medium. For the initiation and growth of the shoots from the potato meristem, the most desirable medium was the diluted MS medium containing 1.0 ppm BA and 0.1 ppm NAA or 0.1 ppm 2.4-D.

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Effects of Increasing Air Temperatures and CO2 Concentrations on Herbicide Efficacy of Acalypha australis and Phytotoxicity of Soybean Crops (대기온도와 CO2 농도 증가에 따른 우점잡초 깨풀의 제초제 약효 및 콩 약해 변화)

  • Hyo-Jin Lee;Hyun-Hwa Park;Ye-Geon Kim;Do-Jin Lee;Yong-In Kuk
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.68 no.3
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    • pp.121-133
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    • 2023
  • The purpose of this study was to improve weed management systems under varying carbon dioxide concentrations and temperatures by evaluating the growth of Acalypha australis and observing the efficacy of four foliar and four soil herbicides, as well as measuring phytotoxicity in soybean crops treated with these herbicides. In both growth chamber and greenhouse conditions, plant height and shoot fresh weight of Acalypha australis increased as temperature increased. The variable to maximum fluorescence ratio (Fv/Fm), relative electron transport rate (ETR), plant height, leaf area, and shoot fresh weight of Acalypha australis were higher at carbon dioxide concentrations of 800 ppm than at 400 ppm. The efficacy of a foliar herbicide, glufosinate, on Acalypha australis was lower at 30℃ than at 20℃ and 25℃ in the growth chamber condition and was also lower at 29℃ than at 21℃ and 25℃ in greenhouse conditions. In contrast, mecoprop efficacy on Acalypha australis was lower at 20℃ and 25℃ than at 30℃ in growth chamber conditions and lower at 21℃ and 25℃ than at 29℃ in greenhouse conditions. Glyphosate efficacy was lower at 21℃ than at 25℃ and 29℃ under greenhouse conditions. With soil herbicides, metolachlor and ethalfluraline, efficacies were higher at relatively high temperatures under both growth chamber and greenhouse conditions. However, in the case of linuron, the difference in efficacy was not observed under varying temperatures in both growth chamber and greenhouse conditions. When ¼ of the recommended glyphosate rates were applied to Acalypha australis, efficacy was lower under 800 ppm carbon dioxide concentrations than under 400 ppm. In contrast, when ¼ of the recommended rate of bentazone was applied to Acalypha australis, efficacy was higher under 800 ppm carbon dioxide concentrations than under 400 ppm. Despite application rates, glufosinate efficacy differed insignificantly under different carbon dioxide concentrations. When applied at ¼ of the recommended rate, the efficacy of ethalfuralin was higher under 800 ppm carbon dioxide concentrations than under 400 ppm. However, efficacies of other herbicides were not different despite varying carbon dioxide concentrations. Soybean phytotoxicity in crops treated with the recommended rate and twice the recommended rate of soil herbicides was not significantly different regardless of temperature and carbon dioxide concentrations. Overall, weed efficacy of some herbicides decreased in response to different temperatures and carbon dioxide concentrations. Therefore, new weed management methods are required to ensure high rates of weed control in conditions affected by climate change.

Developmental Stage and Temperature Influence Elongation Response of Petiole to Low Irradiance in Cyclamen persicum (저광도에 대한 시클라멘 엽병의 발육 단계 및 온도 조건별 신장 반응)

  • Oh, Wook;Kim, Ki-Sun
    • Horticultural Science & Technology
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    • v.28 no.5
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    • pp.719-727
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    • 2010
  • Reduced irradiance promotes shoot elongation depending on developmental stage and environmental factors and decreases plant quality in $Cyclamen$ $persicum$ Mill. To determine the petiole elongation responses to low irradiance, 'Metis Scarlet Red' cyclamen at different developmental stages [juvenile (5-6 unfolded leaves), transitional (1-3 visible flower buds), or mature (1-3 elongating peduncles)] was grown in growth modules at 60 (low light, LL) or 240 (high light, HL) ${\mu}mol{\cdot}m^{-2}{\cdot}s^{-1}$ PPFD within the growth chambers at different temperatures [16/12 (low temperature, LT), 22/18 (medium temperature, MT), or 28/$24^{\circ}C$ (high temperature, HT) (day/night)]. In Experiment I, juvenile plants were either kept in an LL or HL module during the entire treatment of 4 weeks or were transferred to the other module at 1, 2, or 3 weeks after treatment in an MT chamber. In Experiment II, juvenile, transitional, or mature plants were moved to the HL module at 0, 3, 6, 9, or 12 days after being placed in the LL module at the MT chamber and grown for 21 days. In Experiment III, transitional plants were moved to the HL module at 0, 3, 6, 9, or 12 days after being placed in the LL module at the LT, MT, or HT chambers. As the exposure duration to LL increased from 0 to 4 weeks or from 0 to 12 days, petiole length and plant height increased at all temperatures and developmental stages. In Experiment I, the exposure to LL during the latter period, rather than the early period, increased elongation rate. In Experiment II, petiole elongation in transitional plants was more sensitive to LL than juvenile or mature plants during the early period of the treatment for 12 days. In Experiment III, petiole length increased with increasing temperature and exposure duration to LL. Petiole elongation rate at HT increased rapidly from the beginning of LL exposure as compared to LT. Increase of $6^{\circ}C$ in temperature had the similar effect to LL exposure for 3 days in petiole elongation. To conclude, transitional cyclamen under higher temperatures responds more immediately to low irradiance and elongates its petioles.

Mycelial Growth and Extracellular Enzyme Activities of Wood-decaying Mushroom Strains on Solid Media (고체배지 상에서 목재부후성 버섯 균주들의 균사생장 특성과 세포외효소 활성)

  • Jeon, Sung-Min;Ka, Kang-Hyeon
    • The Korean Journal of Mycology
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    • v.42 no.1
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    • pp.40-49
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    • 2014
  • The mycelial growths of wood-decaying mushroom strains collected from Korean forests were investigated on solid media under different culture media and temperatures. Most of strains showed the higher mycelial growth on potato dextrose agar (PDA) than malt extract agar (MEA) or sabouraud dextrose agar (SDA) plates. Except for a few strains, they grew well on PDA at $25^{\circ}C$ and showed a poor growth at low temperature ($10^{\circ}C$) than high temperature ($30^{\circ}C$). All strains showed the carboxymethylcellulase (CM-cellulase) and laccase activities on solid media containing the specific substrates for two different enzymes.

Isolation of Nematophagous Fungi against Root-knot Nematode and Their Growth in Vitro (뿌리혹 선충에 대한 기생 천적 진균 분리 및 이들의 생장에 미치는 환경조사)

  • 정미정;김희규
    • Korean journal of applied entomology
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    • v.27 no.3
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    • pp.149-158
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    • 1988
  • Nematophagous fungi were successfully isolated by baited plating, centrifugation technique of soil, and direct isolation from naturally ingested nematodes. Predominant seven fungi isolated were identified as Artheobotrys arthroboteyides, A.conoides, A. oligospora, Dactylella lobata, Fusatium oxysporum, Monacrosporium ellopsoporum and Harposporium anguillu-lae. Of these, six fungi were tested for cultural characteristics except. H, anguillulae, extre-mely fastidious fungus in artificial media. Among 14 media tested in this experiment, Corn-meal Agar (CMA) and Oatmeal Agar (OMA) were the most suitable media for growing all six nematophagous fungi. Weakly saprophytic M. ellipsospoyum also grew vigoroualy on these two media. The radial growth, dry weight and sporulation of the fungi tested were quite diverse depending on the culture media. D. lobata revealed good growth and abundantly sporulated on Glucoes Peptone Agar (GPA). Although over-all growth of F, oxysporum was not satisfactory on Sucrose Nitrate Agar (SNA), the sporulation was best on this medium. Optimum conditious for mycelial growth and sporulation of nematophagous fungi ranged pH 5-8 and 20-$30^{\circ}C$ on SNA. D. lobata and F, oxysporum grew vigorously and most profusely sporulated on all media tested. They turned out an most promising biocontrol agents for their aggressive growth and sporulation over the ranges of temperature and pH ranges.

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Investigation on Cultural Characteristics of Mycelial Growth by Cordyceps scarabaeicola (풍뎅이동충하초(Cordyceps scarabaeicola)의 균사 생장 조건 규명)

  • Lee, Jae-Keun;Choi, Young-Sang;Sung, Jae-Mo
    • The Korean Journal of Mycology
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    • v.28 no.2
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    • pp.81-87
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    • 2000
  • The optimal synthetic medium, temperature and pH range for the mycelial growth of Cordyceps scarabaeicola were PDA, $25^{\circ}C$, and pH $8.0{\sim}8.5$, respectively. The effects of carbon sources, nitrogen sources and mineral salts on mycelial growth were superior to lactose, ammonium phosphate and $CaCl_2$, respectively. Liquid spawn culture was selected out BM(brown sugar 3%, soybean flour 0.3%, potassium phosphate 0.05%, magnesium sulfate 0.05%) medium.

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Effect of Arbuscular Mycorrhizal Fungi on Growth of Korean Ginseng (Panax ginseng C. A. Mey.) Seedlings (수지상균근균이 인삼 (Panax ginseng C. A. Mey.)의 생장에 미치는 영향)

  • Kil, Yi-Jong;Eo, Ju-Kyeong;Eom, Ahn-Heum
    • The Korean Journal of Mycology
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    • v.41 no.2
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    • pp.81-94
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    • 2013
  • The purpose of this study was to investigate effects of inoculation of arbuscular mycorrhizal fungi (AMF) on growth of Korean ginseng (Panax ginseng C. A. Mey.) seedlings. Five species of AMF (Glomus mosseae, Glomus intraradices, Glomus etunicatum, Acaulospora longula, Acaulospora trappei) were inoculated to ginseng seedlings at 60 days after ginseng seed germination. Dry weights, heights of the plants and chlorophyll fluorescence were measured at 120 days after inoculation of AMF. Dry weights and shoot heights were increased in seedlings inoculated with AMF compared to the controls without AMF. However, chlorophyll fluorescence of seedlings with AMF showed no significant difference compared to the control.

Effects of Medium, Temperature and pH on Mycelial Growth and Cellulase Activity of Ectomycorrhizal Fungi from Korean Forests (우리나라 산림에서 분리한 외생균근균의 균사생장에 있어 배지, 온도, pH의 영향과 셀룰라아제 활성)

  • Jeon, Sung-Min;Kim, Min-Soo;Ka, Kang-Hyeon
    • The Korean Journal of Mycology
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    • v.40 no.4
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    • pp.191-203
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    • 2012
  • Mycelial growth of ectomycorrhizal fungi (27 strains of 8 species) collected from Korean forests was observed on various culture conditions (media, temperature, pHs). After 60 days of incubation, all strains grown on potato dextrose agar (PDA) and modified Melin-Norkran's agar (MMNA), whereas no mycelial growth was observed on malt extract agar (MEA) or sabouraud dextrose agar (SDA) in some strains including Tricholoma matsutake. Mycelial growth on PDA was poor at high temperature ($30^{\circ}C$) than the low temperature ($10^{\circ}C$). The optimal temperature on PDA and pH in potato dextrose broth (PDB) for mycelial growth in most strains were $20-25^{\circ}C$ and pH 4-5, respectively. All strains tested showed the carboxymethyl cellulase (CM-cellulase) activity and the maximal cellullase activity was expressed by the mycelium of T. matsutake (KFRI 1266) on the CMC agar plate with pH 5.0.

Effects of Temperature and Light Intensity on the Growth of Red Pepper(Capsicum annuum L.) in Plastic House During Winter. IV. Growth Responses Influenced by Temperatures and Light Intensities in Growth Chamber (동계 plastic house내 고추(Capsicum annuum L.) 육묘시 온도와 광도가 생장에 미치는 영향 IV. 생장상내 온도 및 광환경 변화에 따른 생장반응)

  • 정순주;이범선;권용웅
    • Journal of Bio-Environment Control
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    • v.4 no.2
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    • pp.125-130
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    • 1995
  • Observations on the seedling growth of red pepper responding to different temperature(10, 20, 3$0^{\circ}C$) and light intensity(5, 15, 25 klux) were made in the growth Chamber during 7 weeks. The results obtained were as follows; 1. Best results of the combinations of temperature and light intensity were obtained from the combinated treatment of 3$0^{\circ}C$ and 25klux. At all of the temperature levels in this experiment, the more the light intensity is high, the more the growth is favor, but at low temperature below 2$0^{\circ}C$ and low light intensity below 15 klux, the growth of red pepper seedlings was decreased markedly. 2. Multiple regression polynomial equations of the characteristics of red pepper seedlings grown in the different combinations of temperature and light intensity fitted well in the plant height, number of leaves, leaf area, stem dry weight and shoot dry weight. 3. Multiple regression polynomial equation to the shoot dry weight was partial differentiated and diagrammatized the response surface using its theoretical value. Light intensity affected more to the shoot dry weight in the temperature below 2$0^{\circ}C$ but above 2$0^{\circ}C$ the role of the temperature showed greatly influence however, interaction effects of light intensity and temperature showed strongly.

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