Browse > Article
http://dx.doi.org/10.4489/KJM.2012.40.4.191

Effects of Medium, Temperature and pH on Mycelial Growth and Cellulase Activity of Ectomycorrhizal Fungi from Korean Forests  

Jeon, Sung-Min (Division of Wood Chemistry & Microbiology, Korea Forest Research Institute)
Kim, Min-Soo (Division of Wood Chemistry & Microbiology, Korea Forest Research Institute)
Ka, Kang-Hyeon (Division of Wood Chemistry & Microbiology, Korea Forest Research Institute)
Publication Information
The Korean Journal of Mycology / v.40, no.4, 2012 , pp. 191-203 More about this Journal
Abstract
Mycelial growth of ectomycorrhizal fungi (27 strains of 8 species) collected from Korean forests was observed on various culture conditions (media, temperature, pHs). After 60 days of incubation, all strains grown on potato dextrose agar (PDA) and modified Melin-Norkran's agar (MMNA), whereas no mycelial growth was observed on malt extract agar (MEA) or sabouraud dextrose agar (SDA) in some strains including Tricholoma matsutake. Mycelial growth on PDA was poor at high temperature ($30^{\circ}C$) than the low temperature ($10^{\circ}C$). The optimal temperature on PDA and pH in potato dextrose broth (PDB) for mycelial growth in most strains were $20-25^{\circ}C$ and pH 4-5, respectively. All strains tested showed the carboxymethyl cellulase (CM-cellulase) activity and the maximal cellullase activity was expressed by the mycelium of T. matsutake (KFRI 1266) on the CMC agar plate with pH 5.0.
Keywords
Cardinal temperature; Cellulase; Ectomycorrhizal fungi; Mycelial growth; pH;
Citations & Related Records
Times Cited By KSCI : 2  (Citation Analysis)
연도 인용수 순위
1 Chudzynski, K., Bielawski, L. and Falandysz, J. 2009. Mercury bio-concentration potential of larch bolete, Suillus grevillei, Mushroom. Bul.l Environ. Contam. Toxicol. 83:275-279.   DOI
2 Dennis, J. 1985. Effect of pH and temperature on in vitro growth of ectomycorrhizal fungi. pp. 1-19. Cannadian Forest Service Pacific Forestry Centre, Canada.
3 Fu, S. Z., Wang, Q. B. and Yao, Y. J. 2006. An annotated checklist of Leccinum in China. Mycotaxon. 96:47-50.
4 Giomaro, G., Sisti, D. and Zambonelli, A. 2005. Cultivation of edible ectomycorrhizal fungi by in vitro mycorrhizal synthesis. Soil Biology. 4:253-267.   DOI
5 Harvey, L. M., 1991. Cultivation techniques for the production of ectomycorrhizal fungi. Biotech. Adv. 9:13-29.   DOI   ScienceOn
6 Jackson, R. M. and Mason, P. A. 1984. Mycorrhiza: Culture and nutrition of sheathing mycorrhizal fungi. The institute of Biology's Studies in Biotechnology. 159:31-33. The Camelot Press Ltd., London, UK.
7 Ka, K. H., Jeon, S. M., Ryoo, R., Ryu, S. H., Kim, M. G, Bak, W. C., Park, J. W., Koo, C. D. and Eom, A. H. 2011. Management of genetic resources of forest microorganisms. Korea Forest Research Institute, Research report. 434: p3. (in Korean).
8 Kim, H. J., Lee, I. S. and Lee, K. R. 1999. Antimutagenic and anticancer effects of Ramaria botrytis(Fr.) rick extracts. J. Korean Soc. Food Sci. Nutr. 28(6):1321-1325.   과학기술학회마을
9 Kasana, R. C., Salwan, R., Dhar, H., Dutt, S. and Gulati, A. 2008. A rapid and easy method for the detection of microbial cellulases on agar plates using gram's iodine. Curr. Microbiol. 57(5):503-507.   DOI
10 Kim, H. J. and Han, S. K. 2009. 200 Mushrooms of forest in Korea. Korea National Arboretum. (in Korean).
11 Kusuda, M., Ueda, M., Miyatake, K. and Terachita, T. 2008. Characterization of the carbohydrase productions of an ectomycorrhizal fungus, Tricholoma matsutake. Mycoscience. 49:291- 297.   DOI
12 Kim, I. Y., Jung, G. R., Han, S. K., Cha J. Y. and Sung, J. M. 2005. Favorable condition for mycelial growth of Tricholoma matsutake. Kor. J. Mycol. 33(1): 22-29. (in Korean).   과학기술학회마을   DOI   ScienceOn
13 Kim, J. H., Yoo, K. H. and Seok, S. J. 2007. Screening test of wild mushroom methanol extracts for fibrinolytic and a-glucosidase inhibitory activity. J. Exp. Biomed. Sci. 13(3):245-249.
14 Kusuda, M., Ueda, M., Konishi, Y., Yamanaka, K., Terashita, T. and Miyatake, K. 2007. Effects of carbohydrate substrate on the vegetative mycelial growth an ectomycorrhizal mushroom, Tricholoma matsutake, isolated from Quercus. Mycoscience. 48:358-364.   DOI
15 Norkrans, B. 1950. Studies in growth and cellulolytic enzymes of Tricholoma. Symb. Bot . Ups. 11:1-126.
16 Laiho, O. 1970. Paxillus involutus as a mycorrhizal symbiont of forest trees. Acta For. Fenn . 106:5-72.
17 Madigan, M. T., Martinko J. M. and Parker, J. 2009. Brock Biology of Microorganisms. 12 edition, pp. 151-167. Pearson/Benjamin Cummings. (in Korean).
18 Marx, D. H. 1969. The influence of ectotrophic mycorrhizal fungi on the resistance of pine roots to pathogenic infections. I. Antagonism of mycorrhizal fungi to root pathogenic fungi and soil bacteria. Phytopathology. 59:153-163.
19 Mortimer, P. E., Karunarathna, S. C., Li, Q., Gui, H., Yang, X., Yang, X., He, J., Ye, L., Guo, J., Li, H., Sysouphanthong, P., Zhou, D., Xu, J. and Hyde, K. D. 2012. Prized edible Asian mushrooms: ecology, conservation and sustainability. Fungal Divers. 56:31-47.   DOI
20 Murat, C., Mello, A., Abba, S., Vizzini, A. and Bonfante, P. 2008. Edible mycorrhizal fungi: identification, life cycle and morphogenesis. In: Mycorrhiza-state of the art, genetics and molecular biology, eco-function, biotechnology, eco-physiology, structure and systematics, 3rd edition, pp. 707-732. Eds. A. Varma. Springer-Verlag, Berlin Heidelberg.
21 Ohta, A. 1997. Ability of ectomycorrhizal fungi to utilize search and related substrates. Mycoscience. 38:404-408.
22 Sanchez, F., Mario Honrubia, M. and Torres, P. 2001. Effects of pH, water stress and temperature on in vitro cultures of ectomycorrhizal fungi from Mediterranean forests. Cryptogamie, Mycol. 22 (4):243-258.   DOI   ScienceOn
23 Terashita, T., Kono, M., Yoshikawa, K. and Shishiyama, J. 1995. Productivity of hydrolytic enzymes by mycorrhizal mushrooms. Mycoscience. 36:221-225.   DOI   ScienceOn
24 Bhat, M. K. 2000. Cellulases and related enzymes in biotechnology. Biotech. Adv. 18:355-383.   DOI   ScienceOn
25 Zhang, Y-H. P., Himmel, M. E. and Mielenz, J. R. 2006. Outlook for cellulase improvement: Screening and selection. Biotechnol Adv. 24:452-481.   DOI   ScienceOn
26 Zimbro, M. J. and Power, D. A. 2003. Difco & BBL manual; manual of Microbiological Culture Media. Becton Dickinson and Company, Sparks.
27 Yamanaka, T. 2003. The effect of pH on the growth of saprotrophic and ectomycorrhizal ammonia fungi in vitro. Mycologia. 95(4):584-589.   DOI
28 Yaoita, Y., Satoh, Y. and Kikuchi, M. 2007. A new ceramide from Ramaria botrytis (Pers.) Ricken. J. Nat. Med. 61:205-207.   DOI
29 Barros, L., Baptista, P. and Ferreira, I. C. F. R. 2006. Influence of the culture medium and pH on the growth of saprobic and ectomycorrhizal mushroom mycelia. Minerva Biotec. 18:165- 170.
30 Berch, S. M., Ka, K. H., Park, H. and Winder, R. 2007. Development and potential of the cultivated and wild-harvested mushroom industries in the Republic of Korea and British Columbia. JEM. 8(3):53-75.
31 Choi, J. H., Ozawa, N., Yamakawa, Y., Nagai, K., Hirai, H. and Kawagish, H. 2011. Leccinine A, an endoplasmic reticulum stress-suppressive compound from the edible mushroom Leccinum extremiorientale. Tetrahedron. 67(35):6649-6653.   DOI   ScienceOn