• Korean Journal of Plant Tissue Culture
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• v.25 no.6
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• pp.477-485
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• 1998

To underatand in vitro regulation of differentiation, the effects of growth regulators and nitrogen source on metabolism of cell wall polysaccharides in suspension culture of kidney bean (Phaseolus vulgaris L.) were investigated. The suspension cells (cell clusters) were directly induced from the epicotyl segments of the seedlings, which were cultivated in MS medium supplemented with 1.0mg/L of 2,4-D and 0.5 mg/L of kinetin. When compared with cell wall sugar contents of the epicotyl segments, the cellulose content of the suspension-cultured cells decreased; while the pectin and hemicellulose content increased; suggesting increases of rhamnogalacturonan I and arabinogalactan IIduring the dedifferentiation, respectively, The effects of growth regulators(2,4-D, 1.0mg/L and kinetin, 0.5mg/L) and nitrogen source (potasium nitrate, 19.0mg/L and ammonium nitrate, 16.5 g/L) in the medium on the proliferation and the turnover of the cell wall polysaccharides were investigated for 30 days. In the medium with growth regulators and without nitrogen source, the proliferation rate was extremely high (16 folds). Growth regulators and nitrogen source increased the pectin content. Analysis of neutral sugar composition of pectin fraction showed that nitrogen source enhanced rhamnose level remarkably, suggesting that rhamnogalacturonan I was the one most likely synthesized. In hemicellulose fraction, growth regulators reduced arabinose level, suggesting that arabinogalactan II was degraded. And nitrogen source reduced galactose level, suggesting that xyloglucan was also degraded.

• Korean Journal of Weed Science
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• v.4 no.2
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• pp.163-168
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• 1984

This study was conducted to find out the effect of paclobutrazol 0.6G and flurprimido 50wp application at 40 days before heading on the characters related with lodging and the growth and yield of paddy rice plants in 1983. The culm length were shortened by paclobutrazol and flurprimido applications and high level of suppression was observed in the 3rd to 5th internodes. Paclobutrazol caused thiner internode and thicker wall of internode than the untreated control, however flurprimido caused thicker wall of internode without change of internode diameter. The breaking weight of lower internode was higher by flurprimido application than those by the untreated control and paclobutrazol application. The lodging indexes effected by both chemicals were remarkably decreased, and also the field lodging was not observed. The leaf area indexes effected by paclobutrazol and flurprimido applications were increased because of the increasing of tillers and green leaves per stem in spite of shortening of lead blades. Paclobutrazol and flurprimido application out-yielded significantly the untreated control lodged at grain filling stage. However in conditions of untreated with no lodging, the yields of these treatments were significantly less than that of untreated control.

• Korean Journal of Medicinal Crop Science
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• v.12 no.1
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• pp.17-23
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• 2004

This study was carried out to obtain the basic information on mating parents and selection for high yielding safflower breeding. Thirty nine world safflower lines, which had collected from 13 countries of origjn, were evaluated for major agronomic characters, correlation and path-coefficients. The results obtained were summarized as follows. Days to flowers ranged from 83 days to 100 days. Days to flower of most foreign lines were later than that of domestic lines. Plant height ranged from 75 cm to 162 cm. The plant height and stem diameter of most foreign lines were higher than those of domestic lines. The leaf number and size of domestic lines were much more than those of foreign lines. The total branch numbers of foreign lines were more than domestic lines. Particularly, the number of third branch ranged from 0 to 25 and there were none in domestic lines. The number of corolla of foreign lines was much more than that of domestic lines. The yield of foreign lines collected from Central Asia and United States of America were more than any others. Significantly positive correlations were observed between yield and days to flower, stem diameter, the number of branch and the number of corolla. In the analysis of path-coefficient, the highest direct effects on yield was the number of corolla followed by the number of total branch, whereas those of the number of second branch and stem diameter were negative.

• Journal of The Korean Society of Grassland and Forage Science
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• v.11 no.1
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• pp.6-11
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• 1991

In order to classify the varieties by the characters to evaluating potential winter hardiness was examined using the relationships between autumn growth of seedling plants and the dry weight of plant(DW), and yield components of 1st cutting in 16 varieties of perennial ryegrass grown under individual plant basis.The results are summarized as tollows:1. The dry weight of plant(L)W) was positively correlated with the number of tillers of seedling plants, but negatively correlated with seedling vigors of plants in autumn growth. 2. The dry weight of plant(L)W) indicated positively significant correlations with the dry weight of heading tillers(HDW), number of tillers per plant(NT), number of vegetative tillers(VNT), number of heading tillers(HNT), dry weight of a tiller(WT) and heading rates(HR) of 1st cutting.3. Relative tiller appearance rates(RTAK) corresponded with the seasonal patterns of tillering ability. Thevariety with a low values of relative tiller appearance rates(RTAR) showed vigorous tillering ability inautumn growth, but the variety with a high values of relative tiller appearance rates(KTAR) showedvigorous tillering ability in spring growth.4. The dry weight of plant per relative tiller appearance rates(DW/RTAR) indicated positively significant correlations with the dry weight of plant(DU7, plant length(PL), dry weight of heading tillers(HDW), number of tillers per plant(NT), number of heading tillers(HNrr), dry weight of a tiller(WT) and heading rates(HR) of 1st cutting.5. The varieties were classified into 2 groups by the dry weight of plant per relative tiller appearance rates(DW/RTAR). Thus. Manhattan, P-2, Tonga, Tove, Hella. I'eramo and Fiesta belong to the group of DW/RTAII>l. 0, and Maprima, Caliente, Tempo, Gambit, Magella, Prana. Reveille, Barlet and Bravo belong to the group of DW/RTAR

• KOREAN JOURNAL OF CROP SCIENCE
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• v.33 no.4
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• pp.329-335
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• 1988

[n order to find out the effects of nitrogen fertilizer and planting density on the leaf characteristics of high yielding cultivars of rice, Dongjin (Japonica type) and Samkang (Tongil type) were .grown under two nitrogen levels (150 and 250 kg N/ha) and three planting densities (30 ${\times}$ 15 cm, 30 ${\times}$ 12 cm, 30 ${\times}$ 9 cm. Leaf blade, sheath and dry weight of individual leaf. the number of green leaves after flowering were measured. Samkang was characterized as longer and wider leaf blade and sheath than Dongjin, less sensitive to nitrogen fertilizer and planting density, and higher proportions of green leaves after flowering. These morphological characteristics of Samkang caused an increase of grain yield by 30 per cent.

• Horticultural Science & Technology
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• v.29 no.3
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• pp.267-272
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• 2011

Several reports indicated that astaxanthin and zeaxanthin have more active anticancer activity than pro-vitamin A carotenes. ${\beta}$-carotene hydroxylase is a key enzyme to synthesize zeaxanthin and astaxanthin in carotenoids biosynthesis pathway. We isolated the ChyB gene encoding ${\beta}$-carotene hydroxylase from tomato leaves. The ChyB gene (1.5Kbp) fragment was cloned into the binary vector and designated to pIG121-ChyB-tom. Agrobacterium-mediated infiltration was used for transient assay in Nicotiana benthamiana. Leaf samples were collected 0, 1, 2, 3 days after infiltration (DAI). RT-PCR result showed that the expression of ${\beta}$-carotene hydroxylase transcripts was not detected in control (0DAI), but its expression was detected after 1 DPI and increased later on. When the activity of ${\beta}$-carotene hydroxylase was measured, the 1,1-diphenyl-pricryl hydrazyl (DPPH) radical scavenging activity (27%) at 2 DAI was significantly higher than that (21%) at 0 DAI. These results indicated that anti-oxidant activity dramatically increased at 2 DAI in tobacco leaves was due to over expression of tomato ${\beta}$-carotene hydroxylase. These results can be the foundation to develop tomato cultivars with high oxy-carotenoids content using the ChyB gene transformation.

• Korean Journal of Agricultural and Forest Meteorology
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• v.1 no.2
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• pp.90-96
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• 1999

Foliar and physiological characteristics of Schisandraceae plants distributed in Korea were investigated to elucidate differences in growth and the variations of inter and intra species of the plants. For this study, three species of Schisandraceae plants were used; i) Schisandra chinensis, decidious broad-leaved tree, ii) S. nigra, decidious broad-leaved tree, including monoecious, male and female types, ii) Kadsura japonica, evergreen broad-leaved tree. There were no significant differences in leaf length and width among three species, but leaves of Schisandra sp. plants were shorter and wider relative to those of K. japonica. The length of petiole of Schisandra sp. plants was greater than that of K. japonica and the difference was evident between female type of S. chinensis and K. japonica as longer as two times. Schisandra sp. trees showed larger size of stomata, but fewer numbers of stomata, compared with K. japonica. Although substantial changes in leaf morphology were not found in the Schisandraceae plants grown in different environmental conditions, the foliar length of K. japonica was seemed to be increased with increasing an altitude. Chlorophyll contents were also variable in inter and intra species and in, general K. japonica showed higher levels of chlorophyll contents relative to Schisandra sp. plants. K. japonica also showed higher light compensation point(LCP), light saturation point(LSP) and $CO_2$ assimilation rates at LSP, compared with Schisandra sp. trees. However, Schisandra sp. trees showed lower respiration per photosynthesis rates at LSP. The finding suggested that Schisandra sp. trees were acclimated to the growth conditions of lower light intensity with more effective photosynthetic activity. This was also confirmed by the fact that Schisandra sp. plants grown in shading places of a forest demonstrated the elevated rates of net $CO_2$ assimilation under the conditions of low light intensity.

• Horticultural Science & Technology
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• v.19 no.1
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• pp.29-33
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• 2001

This study was carried out to discriminate potato cultivars and breeding lines by specific molecular markers using random amplified polymorphic DNA (RAPD) analysis. The genotypes of potatoes used for analysis were eight cultivars and five breeding lines. Some of those show much phenotypic resemblances among them because 'Jopung', 'Daekwan70', 'Gawon', and 'Daekwan72' have immediate parental relationship with 'Superior', 'Irish Cobbler', 'Namsuh', and 'Atlantic', respectively. So, there are many difficulties to distinguish the varieties by the morphological characteristics. Three URP primers, URP2, URP4, and URP8 were selected for promising primers to discriminate potato genotypes or cultivars. The three URP primers were shown very high reproducibility because of the relatively high annealing temperature and long primer size. Although the results of similarity analyses did not always reflect the genetic relationship between potato varieties, the reproducible pattern of amplified DNA bands by URP primers showed possibility for molecular markers for discrimination of potato genotype or cultivar.

• Journal of Life Science
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• v.12 no.3
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• pp.256-263
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• 2002

This study was carried out to estimate the degree of heterosis and heterobeltiosis, and the heritability and genetic correlation in F$_1$hybrid produced by reciprocal crosses between two six-rowed hulled barley (Gangbori and Olbori) and three six-rowed naked barley (Saechalssalbori, Saessalbori, and Moodeongssalbori). Heterosis was observed positively in traits such as cults length, spike length, awn length, leaf length, leaf width, number of spikes per plant, number of grains per spike, and 1000 grain weight. Heterosis and heterobeltiosis from the crosses with hulled barley as a material parent showed higher performance in culm length, leaf length, leaf width, number of spike per plant, number of grains per spike. The hulled grain characteristics is dominant over the naked one. The highest heritability was confirmed in leaf width with a value of 94.5% and overall high heritability was observed in most traits with the range from 76.7% to 94.5%. In an analysis of correlation coefficient, higher genetic correlations were observed between leaf length and number of spikes per plant(0.970), between cults length and number of grains per spike(0.963), and between awn length and leaf length(0.862) in the forward crosses in which hulled barley was maternal. In the reverse crosses, higher genetic correlation was observed between culm length and spike length(0.926), between awn length and leaf length(0.922), and between number of spikes per plant and number of grains per spike(0.713).

• Journal of Ginseng Research
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• v.26 no.2
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• pp.85-88
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• 2002

This study was carried out to obtain information of the photosynthetic rate at various temperature and light intensity, stomata, chlorophyll, specific leaf weight, characteristics of aerial part and root in ginseng new cultivars developed by pure line selection. The light saturation point of leaves in new cultivars and Jakyungjong were 15,000 lux, and the optimum air temperature on the photosynthesis of new cultivars and Jakyungjong were 20$\^{C}$. The photosynthetic rates were increased in order of Jakyungjong, Gopoong, Chunpoong and Yunpoong. The dark respiration rate of leaves in ginseng cultivars were increased according to the increasing of temperature, and the dark respiration rate of leaves of Yunpoong was the highest among cultivars. The specific leaf weight (SLW) were increased in order of Jakyungjong, Yunpoong, Gopoong, Chunpoong, but total chlorophyll contents were not different among cultivars. Stomata frequency of Yunpoong was the highest being 69.2ea among cultivars, while the length of stomata was reverse. Yunpoong was superior in aerial part among ginseng cultivars : the number of stem was 1.8ea, the number of palmately leaves was 7.7ea, the number of leaflets was 41.0ea, leaf area was 12.3 dm$^2$ The root weight were increased in order of Jakyungjong, Gopoong, Chunpoong and Yunpoong. Chunpoong and Gopoong hove good root shape the length of tap root in Chunpoong and Gopoong were the longest being 6.5 cm and 6.8 cm respectively, but that in Yunpoong was the shortest being 4.4 cm.