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Enhanced Antioxident Effect by over Expression of Tomato β-carotene Hydroxylase Gene (ChyB) Using Agrobacterium-infiltration in Tobacco Plant  

Choi, Yoon-Jeong (Department of Horticulture and Life Science, Yeungnam University)
Yoon, Kyung-Young (Department of Food and Nutrition, Yeungnam University)
Yun, Hae-Keun (Department of Horticulture and Life Science, Yeungnam University)
Suh, Sang-Gon (Department of Horticulture and Life Science, Yeungnam University)
Moon, Yong-Sun (Department of Horticulture and Life Science, Yeungnam University)
Publication Information
Horticultural Science & Technology / v.29, no.3, 2011 , pp. 267-272 More about this Journal
Abstract
Several reports indicated that astaxanthin and zeaxanthin have more active anticancer activity than pro-vitamin A carotenes. ${\beta}$-carotene hydroxylase is a key enzyme to synthesize zeaxanthin and astaxanthin in carotenoids biosynthesis pathway. We isolated the ChyB gene encoding ${\beta}$-carotene hydroxylase from tomato leaves. The ChyB gene (1.5Kbp) fragment was cloned into the binary vector and designated to pIG121-ChyB-tom. Agrobacterium-mediated infiltration was used for transient assay in Nicotiana benthamiana. Leaf samples were collected 0, 1, 2, 3 days after infiltration (DAI). RT-PCR result showed that the expression of ${\beta}$-carotene hydroxylase transcripts was not detected in control (0DAI), but its expression was detected after 1 DPI and increased later on. When the activity of ${\beta}$-carotene hydroxylase was measured, the 1,1-diphenyl-pricryl hydrazyl (DPPH) radical scavenging activity (27%) at 2 DAI was significantly higher than that (21%) at 0 DAI. These results indicated that anti-oxidant activity dramatically increased at 2 DAI in tobacco leaves was due to over expression of tomato ${\beta}$-carotene hydroxylase. These results can be the foundation to develop tomato cultivars with high oxy-carotenoids content using the ChyB gene transformation.
Keywords
anticancer effect; functional food; 'Momotaro' tomato;
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