• Horticultural Science & Technology
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• v.19 no.4
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• pp.476-482
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• 2001

This experiment was conducted to evaluate the effect of slow-release fertilizer application on quality elevation of onion (Allium cepa L.) in southwestern sea. The slow-release fertilizers used were NIAST (National Institute of Agricultural Science and Technology) I, II, CDU (Crotonylidene Diurea), UF (Urea-Formaldehydes), and IBDU (Isobutylidene Diurea) 30, 50. Growth of the onion plant treated with slow-release fertilizer showed better than the control. However, a rate of infected plant to downy mildew was low as compared with control. Chlorophyll and total nitrogen contents were high in control, whereas firmness, soluble solid content, number and thickness of scaly leaves were high in slow-release fertilizer plot. In early maturing onion, total marketable bulb yield was higher in slow-release fertilizer plot than all other fertilizer treatments. Total marketable bulb yield in late maturing onion treated with slow-release fertilizer was slightly decreased. Decaying loss and the rate of sprouting during storage period decreased in slow-release fertilizer application. In summary, application of slow-release fertilizer increased marketable yield, storability after harvest and also reduced labor needs for top dressing.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.58 no.2
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• pp.169-176
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• 2013

Preharvest sprouting seriously reduces milling and baking quality of hard winter wheat (Triticum aestivum L.) grain. To determine the effect of nitrogen fertilizer application on decreasing of preharvest sprouting, several levels of N-fertilization were conducted in two winter wheat cv. Keumkang and Jokyung, grown in Iksan. Nitrogen fertilization is used to increase grain yield and protein content. Grain yield increased at 108kg/ha (50% increased nitrogen to the standard) application and decreased as more nitrogen was applied. There was a linear increase in grain protein contents with increasing level of nitrogen application. Germination rate, germination index and ABA sensitivity were gradually reduced by increasing of nitrogen application level. Preharvest sprouting showed a significantly correlation to germination rate but could not be correlated to protein content and falling number. A significant positive correlation was detected between preharvest sprouting and different additional nitrogen fertilizer levels.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.65 no.4
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• pp.365-376
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• 2020

Immature embryogenesis is a useful process in wheat tissue culture, including transgenic technology, because of its high regeneration efficiency compared to that in other tissues. However, it is a very labor-intensive and time-restrictive method, because the preparation of immature embryos is limited to the optimal time after flowering. In this experiment, 'Speed Breeding', a breeding technique that accelerates breeding generation advancement by extending the photoperiod, was applied to the wheat variety 'Bobwhite'. A controlled growth room was constructed by adjusting the photoperiod (22-hour light/2-hour dark) using LED lights at temperature of 22℃. After vernalization of the Bobwhite seeds at 4℃ for 4 weeks, the seedlings were grown in a controlled growth room and a greenhouse to compare the heading date. In both conditions, calli were induced from immature embryos on the 11^th day after flowering. After 4 weeks, the calli were transferred to a regeneration medium. Regeneration efficiencies under greenhouse conditions and Speed Breeding conditions were determined as 45.05% and 43.18%, respectively. Antioxidant enzyme activity and reference gene expression analysis were performed to confirm the presence of stress due to an extremely long-day photoperiod. As a result, the antioxidant enzyme activity was not distinguished from that of the greenhouse condition. The reference gene expression analysis revealed that the PsaA and CDC genes were highly expressed under the Speed Breeding condition. However, expression of PsbA was similar expression in both conditions. These results will provide useful information for the application of immature embryogenesis to the wheat transformation system.

• Current Research on Agriculture and Life Sciences
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• v.10
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• pp.125-135
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• 1992

Nine strains of xyl mutants that could not utilize xylose as a carbon source were isolated from E. coli JM109 by the treatment of NTG in order to investigate the regulation of xylose operon and to use recipient cells for the cloning of xylR gene. For the characterization of all isolated mutants, colony colors of all mutants on MacConkey-xylose and MacConkey-xylulose agar plate were observed for the utilization of xylose and xylulose, and the growth level and the activity of xylose isomerase and xylulokinase were determined in need. The isolated xylR/T mutants formed the white colony on MacConkey-xy-lose and MacConkey-xylulose agar plate. They did not detect the activity of xylose isomerase, and the activity of xylose isomerase was not restored in transformants of xylR/T mutant with pEX13 which contained xylA gene. xylR and xylT mutants were classified from xylR/T mutants depending upon the growth level in minimal medium. xylT mutants; DH13, DH121 and DH125 could grow a little in that medium, but xylR mutants; DH10, DH53, and DH60 could not grow that medium.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.37 no.3
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• pp.244-249
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• 1992

To investigate the varietal variations in absolute density of milled rice grain and its relations with other grain characters, the 235 rice varieties which consist of 30 Tongil type rices, 72 Japonica rices and 133 Indica rices were tested on grain size, volume, hardness, chalkiness and 1000 grains weight of brown rice, ADV, amylose content, starch composition on SEM and absolute density of milled rice grain using completely ripened grains. Average absolute density of milled rice grain was 1.496g / cm$^3$ in Tongil type rices, 1.506g / em in Japonica rices, and 1.500g / cm$^3$ in Indica rices. It was correlated positively with days to heading and grain hardness, and negatively with chalkiness, volume, grain weight and grain length of brown rice. Regression analysis indicated that grain volume and weight were the major characters affecting the density. However, since the absolute density of milled rice grain did not show great varietal variations it might not seem important as one of the characters contributing to the grain yield, while it could be a factor affecting the grain quality because there were definite varietal differences even though small. The microscopic feature of starch composition on SEM revealed differences between clear and chalky parts of the grain in shape and compactness of starch composition but did not discriminate between high and low-density grains.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.37 no.4
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• pp.347-354
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• 1992

Late harvest in peanut has often resulted in reduced yield and dissipation of labor by virtue of increasing over-matured pods causing the pod shattering. Present study was conducted to obtain a basic information for deciding optimum harvest time of the peanut in Chungbuk province by examination of yield components at different harvest time of leading cultivars Saedl and Youngho cultivated with vinyl mulching or non-mulching conditions. Peg number and pod number were significantly increased by vinyl mulching and also significantly different by the harvest time. Pod number increased with the lapse of days after flowering was gradually decreased from 100 days after flowering in saedl and 110 days in Youngho. Number of seed-bearing pods and matured seed percent were significantly increased by vinyl mulching and had tendency to increase with the lapse of days after flowering. There was no significant difference in number of over-matured pods and pod shattering percent between vinyl mulching and non-mulching. They increased drastically in 110 days after flowering in Saedl, but in 120 days after flowering in Youngho, Increase in 100 seed weight by vinyl mulching was statistically nonsignificant in both varieties, however, total seed yield was significantly increased by vinyl mulching, showing maximum yield in 100 days after flowering in the variety Saedl and 110 days in Youngho, respectively, Seed yield was negatively correlated to peg number and positively correlated to pod number, seed-bearing pod number, pod shattering percent, matured seed percent and 100 seed weight, respectively.

• Microbiology and Biotechnology Letters
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• v.36 no.2
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• pp.101-105
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• 2008

In order to obtain yeast cells producing a bacteriocin OR-7, the 180 bp polynucleotide corresponding to the OR-7 gene including codons for start and stop was chemically synthesized and cloned into pAUR123, an yeast expression vector. Transformed yeast cells exhibited growth inhibition of Bacillus subtilis, Campylobacter jeuni, Escherichia coli and Pseudomonas aeruginosa. This result indicates that yeast cells producing OR-7 possess bacteriocidal properties against both Gram positive B. subtilis and Gram negative C. jejuni, E. coli and P. aeruginosa cells. The recombinant yeast strain constructed in this study can be applied in the food preservative or animal feed.

• Korean Journal of Environment and Ecology
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• v.7 no.1
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• pp.10-21
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• 1993

Artificial acid rain (pH 3.0, 4.0 and 5.0) and ground was treated on the seeded pots of 4 species to examine its effects on germination and survival rate, seedling growth and contact angles on needle surface. Artificial acid rain was prepared by diluting sulfuric acid with ground water and ground water (pH 6.5) was used as control. Artificial acid rain was sprayed to seeded pots two or three times per week for growing season, one times per week for winter seasons. About 5mm of artificial acid rain was treated each time from early April, 1991 to early October, 1993. Germination and survival rate, soil acidity, seedling growth and contact angles on the needle surface were measured and compared among the treatments. The results were summarized as follows ; Artificial acid rain might have positive effects on growth-related characteristics of the seedlings in the first and second year of acid rain treatment, and the effects were differed among four species. All growth-related characteristics of the seedlings in third year, however, decreased with decrease of pH values of artificial acid rain. This was considered to the results of acidic accumulation over soil buffer capacity. Needle injury and biomass (defoliation) was correlated with the pH values of artificial acid rain, and this character might be a good criteria for early diagnosis of acid rain injury. The differences of soil acidity were significant among the treatments for all species. Contact angles between needle surface and water droplet decreased with decrease of pH values of artificial acid rain. Measuring and comparing contact angles might be very good criteria for early diagnosis of acid rain injury.

• Korean Journal of Agricultural Science
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• v.20 no.2
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• pp.133-144
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• 1993

Calli were induced on microtuber disks of potato(S.tuberosum) infected with three binary vectors transconjugated with C58, A281 and LBA 4404 of Agrobacterium tumefaciens and pBI121. The frequency inducing callus was the highest by infection of C121 carrying pC58 and pBI121, and shoots were differentiated on the calli without any hormonal application. Transformed calli were selected by their resistance to kanamycin and identified by GUS activity. The frequency of callus formation by infection of binary vector strain was affected according to the hormonal application.

• Journal of Life Science
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• v.18 no.11
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• pp.1507-1512
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• 2008

In spite of long research period for Salmonella typhi, little information is known about the pathogenesis mechanism of human typhoid fever caused by S. typhi due to lack of infection model in animals. A wild-type of S. typhi Ty2 strain requires cysteine to grow on minimal media. We hypothesized that this cysteine requirement may restrict colonization of S. typhi in animals during infection process. Among the S. typhi strains carrying Salmonella typhimurium genomic library, we have isolated three S. typhi transformants growing on minimal media without cysteine. Although there were three ORFs in DNA of pBP71, the STM1490 ORF complemented cysteine auxotrophy of S. typhi. Analysis of the deduced amino acid sequence of the STM1490 homolog in S. typhi revealed that there are differences in two amino acids. Plasmids containing amino acid substitutions in STM1490 supported S. typhi growth on minimal media without cysteine, indicating irrelevance of these two amino acids to STM1490 function. These results tells us that there are other factors or systems involved in cysteine requirement of S. typhi.