• Microbiology and Biotechnology Letters
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• v.14 no.3
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• pp.239-244
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• 1986

To develop the potential use as new host strain for gene cloning, alkaline-tolerant isolates from soil were examined for amylase activity, protease activity, antimicrobial activity and transformability by using plasmid pUB 110. Of these strains, one was selected and identified as Bacillus sp. YA-14. in the enzymatic properties of Bacillus sp. YA-14 the optimal conditions for the reaction of amylase and protease were at pH 0.8 and pH 7.5 respectively. The antimicrobial activity of Bacillus sp. YA-14 was also found. For the transformation, Bacillus sp. YA-14 was cultured to late logarithmic growth phase ai 37$^{\circ}C$ in modified SPI medium (pH 8.0) containing 0.4% MgSO$_4$. The presence of pUB 110 plasmid DNA in transformants was confirmed by electrophoresis and stably maintained in the new host.

• Journal of Plant Biotechnology
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• v.43 no.3
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• pp.376-382
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• 2016

DNA methylation regulations gene expression, thus having pivotal roles in a myriad of physiological and pathological processes. In this study, the morphology and stress tolerance of transgenic rice overexpressing NtROS2a were determined. Transgenic plants exhibited less and shorter lateral shoots. Under various treatments, rice overexpressing NtROS2a showed alleviation of damage symptoms with higher survival rate. After drought and re-watering treatment, transgenic rice seedlings restored their normal growth. However, wild type plants could not be rescued. These findings indicate that overexpression of NtROS2a gene in rice seedlings can increase their tolerance to drought stresses.

• Korean Journal of Plant Tissue Culture
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• v.28 no.4
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• pp.197-200
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• 2001

Agrobacterium tumefaciens MP90 harboring PAT (phosphinothricin acetyltransferase) and NPTII-GUS gene were used for the genetic transformation of lettuce (Lactuca Sativa L.). Shoot regeneration from cotyledon explants were obtained from the MS medium supplemented with 0.1 mg.L$^{-1}$ NAA, 1.0 mg.L$^{-1}$ 2ip, 50 mg.L$^{-1}$ kanamycin and 500 mg.L$^{-1}$ carbenicillin after cocultivation with A. tumefaciens for 2 days. Kanamycin resistance test of transgenic plants indicated that the NPTII gene was integrated into the lettuce genome and was stably expressed. PCR and northern blot analysis indicated that bialaphos resistance gene (PAT) was stably integrated into the lettuce genome. The transgenic plant sprayed with Basta (1500x) remained healthy with continuous growth, while the control group exhibited fatality.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.36 no.2
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• pp.143-146
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• 1991

The present experiment was carried out to investigate the effects of direct sowing time culture of Perilla frutescens var. acuta KUPO on growth yield and it's components. The number of days from seeding to emergence was recognized linear negative correlation between seeding dates. Stem length was the longest in Apr. 1 seeding, and was short in the early, and the late seeding. Stem diameter was the thickest in Apr. 1 seeding, and was thin in the early, and the late seeding. Dry weight of leaf and stem was increased on Apr. 1 seeding. Grain yield was the highest in Apr. 1 seeding date. Therefore, optimum time for direct sowing was on Apr. 1.

• Korean Journal of Plant Resources
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• v.14 no.1
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• pp.60-64
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• 2001

This study was carried out to investigate effects of sowing dates and method on major agronomic characteristics of Dendropanax morbifera $L_{EV}$. in southern area of Korea. The number of days from sowing to emergence was recognized liner negative correlation between sowing days. Dendropanax morbifera was sowed at seven different dates(from Dec.20 to Feb.20 at 30 days interval, from Mar, 1 to Apr. 15 at 15 days interval) in pot culture. In sowing date at Mar. 15, germination rate and early growth were good in pot. Dendropanax morbifera was sowed at five different dates(from Dec.20 to Apr.15 at 15 days interval) in field culture. In sowing date at Apr.15, germination rate and early growth were good in field culture.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.28 no.1
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• pp.100-106
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• 1983

This experiment was conducted to study a cause of lodging on culm internode. The rice varieties were undergone on injuries by typo on 'Agnes' on Jeonbug Provincial in 1981 year. Long-culmed varieties had extreme lodging injury but short-culmed varieties were not serious. The lodging had weaken tolerance when culm internode was long, weight of internode per unit length was light, and culm diameter and culm wall were thin. The breaking weight of internode was light in lodging rice field. The internode length and the weight of internode per unit length between the breaking weight of internode had significant correlation. Lodging had high tolerance when the total nitrogen was low, phosphate, silica, cellulose and starch contents were high in internode. The difference of culm length and the weight of internode per unit length was high relationship with lodging.

• Proceedings of the Korean Society of Crop Science Conference
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• 2022.10a
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• pp.148-148
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• 2022

콩[(Glycine max(L.)]은 우리나라에서 벼와 더불어 주요한 식량작물이다. 농촌진흥청에서는 콩 생육데이터를 수집하여 생산성 향상모델을 개발하기 위해 '농업빅데이터수집및생산성향상모델개발' 사업을 수행하고 있다. 수집되는 콩 데이터는 농가정보, 콩 생육정보, 토양정보 부분으로 구성되어 있으며 농가정보는 시군, 시군구, 품종, 파종량, 종자확보경로 등이 수집되고 있다. 그리고 콩 생육정보는 경장, 줄기굵기, 마디수, 가지수, 꼬투리수, 꼬투리립수, 개체당 입수, 종실수량 등이 수집되어 있다. 토양정보는 수분, 지온, EC 등이 수집되고 있다. 주요 항목의 평균은 경장 47.4 cm, 줄기굵기 11.1 mm 마디수 12.7 개, 꼬투리수 54.0 개, 꼬투리립수 2.7 개, 종실수량 227.9 kg/10a 정도이며 토양수분은 26.3 %, 지온은 27.1 ℃ EC는 2.58 ds/CM 정도이다. 주요 형질의 상관관계는 종실수량과 개체당 협수가 0.651로 나타났으며 가지수, 꼬투리수, 개체당협수와 줄기굵기는 각각 0.783, 0.653, 0.663 정도로 나타났다. 추후 이를 기반으로 다중회귀 등 분석 가능한 방법(머신러닝 등)을 적용하여 콩수량을 예측할 수 있는지 검토할 필요가 있다. 또한 본 사업으로 수집된 자료를 분석하여 콩 수량에 영향을 미치는 주요 요인을 평가한 결과는 콩 생산성 향상을 위한 모델 작성에 중요한 자료로 활용될 수 있을 것으로 예상된다.

• Korean Journal of Plant Resources
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• v.26 no.2
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• pp.271-283
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• 2013

Morphology of Korean Scrophularia species were reviewed to clarify taxonomic characters, their variations and taxon delimitation. Habitats, root forms, foliaceous types, glandular trichomes on stems and leaves, flowering seasons, calyx shapes, corolla colors and shapes, as well as ovary basal shapes are diagnostic characters to classify series in this genus. From the result, 1) S. alata is distinguished from its closely related species S. takesimensis by lengths of stem ribs, widths of petioles, and types of corolla lobes; 2) S. buergeriana is characterized by spike-like inflorescences, short peduncles and pedicels, and yellowish green corollas; 3) lanceolate-shaped leaves and calyces, and axillary-like cyme inflorescences delimitate S. koraiensis easily from the other taxa; 4) smaller leaves discriminate S. kakudensis var. microphylla from S. kakudensis var. kakudensis, and 5) S. cephalantha is distinguished from S. kakudensis by broadly ovate to sub-orbicular shaped leaves, earlier flowering seasons, and smaller numbered flowers on shorter inflorescences. In conclusion, the Korean Scrophularia is classified as 7 taxa, 6 species and 1 variety in 2 series. Keys to the series and taxa are provided.

• Journal of Plant Biotechnology
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• v.42 no.3
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• pp.180-185
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• 2015

Leaf discs from 'Yeobong' and 'Maehyang' strawberry plants were used as explants for transformation. The Agrobacterium tumefaciens strain EHA105 harboring the monellin gene under the control of the CaMV 35S promoter was used in co-cultivation experiments. The frequencies of callus formation and plant regeneration from leaf explants after co-cultivation in 'Yeobong' were higher than those of 'Maehyang'. These transgenic plants showed normal growth patterns and flowering. PCR and Southern hybridization confirmed that 1 to 2 copies of the monellin gene were integrated into genome of the transgenic strawberry plants. Northern blot analysis confirm that the transcripts were expressed in transgenic strawberry plants. Although long-term subcultured transgenic strawberry plants showed a phenomenon to escape the transgene, the transformation system established in this study provides new opportunities for genetic improvement of strawberry plants.

• Korean Journal of Plant Tissue Culture
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• v.26 no.3
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• pp.163-169
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• 1999

This study was conducted to produce herbicide resistant plants by transferring phosphinothricin acetyltransferase (PAT) gene into Populus alba $\times$ Populus glandulosa No .3 using Agrobacterium tumefaciens MP 90/PAT. Leaf segments from in vitro grown shoots of hybrid poplar No. 3 were soaked in a AB medium containing Agrobacterium tumefaciens MP 90/PAT for 10 min and cocultivated for 2 days on MS medium containing 1.0 mg/L 2,4-D and 0.2mg/L kinetin (CIM). Putative transformed calli could be selected after cocultivation of leaf segments on CIM supplemented with 50mg/L kanamycin and 500mg/L cefotaxime for 3 weeks. The selected calli were cultured on CIM supplemented with 50 mg/L kanamycin and 500 mg/L cefotaxime for 5~8 weeks before transfer to WPM containing 1.0mg/L zeatin, 0.1mg/L BAP, 50 mg/L kanamycin and 500mg/L cefotaxime for shoot regeneration. Shoots were regenerated from the callus after 4 week cultivation, and the regenerants were grown on the same medium for 7~l0 weeks. The plants rooted on 1/2 WPM containing 0.2 mg/L IBA and 50 mg/L kanamycin. To confirm the gene insertion into plants, GUS activity was detected by histochemical assay in the transformed plants. Finally, the presence of both NPT II and PAT genes from the transgenic plants were confirmed by PCR amplification with the gene specific primers and subsequent PCR-Southern blot with DIG-labeled PAT gene probe. After acclimatization in pots for 4 weeks, the plants were sprayed by 3 mL/L of Basta to test resistance to the herbicide. The transgenic plants remained green, whereas all the control plants died after one week.