• Title/Summary/Keyword: 생산과정의 분화

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Dedifferentiation State Specific Increase of Trypsin- and Chymotrypsin-like Protease Activities during Urodele Limb Regeneration and Their Enhancement by Retinoic Acid Treatment (유미양서류 다리 재생 기간중 탈분화 시기 특이적 트립신, 키모트립신 유사 단백질 효소의 활성도 증가)

  • 이은호;김원선
    • The Korean Journal of Zoology
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    • v.39 no.1
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    • pp.65-74
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    • 1996
  • Treatment of regenerating amphibian limbs with retinoic acid (RA) is known to induce paftern duplication, which is closely related to the extent of dedifferentiation. In the present study, the activities of trypsin- and chymotrypsin-like proteases are examined to delineate a possible role in the process of dedifferentiation in the regenerating limbs of urodeles, the Korean salamander (Hynobius leechii) and the Mexican axolod (Ambystoma mexicanum). Specifically, we were interested to know if there is any correlation between trypsin- and chymotrypsin-like protease activities and the state of dedifferentiation which is augmented by RA treatment. We were also interested in expoloring if there is any species-specific difference in the profile of enzyme activities during limb regeneration. The results showed that the activities of these two enzymes reached a peak level at dedifferentiation stage, and RA treatment caused elevation of their activities, especially in the case of trypsin-like protease. The increase of trypsin-like protease activity after RA treatment was pronounced in the Korean salamander, which might reflect a species-specific responsiveness to RA. The present results imply that trypsin and chymotrypsin or similar proteases may play an active role in the process of dedifferentiation in regenerating limbs, and that trypsin or trypsin-like eryrymes might be involved in the RA-evoked enhancement of dedifferentiation which precedes overt pattern duplication.

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Establishment of Callus Induction and Plant Regeneration System from Mature Seeds of Miscanthus sinensis (억새(Miscanthus sinensis) 성숙 종자로부터의 캘러스 유도 및 식물체 재분화 체계 확립)

  • Cho, Joon-Hyeong;Byeon, Ji-Hui
    • Korean Journal of Plant Resources
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    • v.24 no.5
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    • pp.628-635
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    • 2011
  • This study was conducted to establish the tissue culture system for Korean domestic Miscanthus sinensis, which is used in various purposes such as forage, and bio-energy resources. With the mature seed of Miscanthus, optimum concentrations of plant growth regulators were identified for an efficient callus induction and regeneration. Among the treatments of 1~10 $mg{\cdot}L^{-1}$ 2,4-D, IBA, or NAA, callus induction rate was highest (85.3%) on MS medium containing 5 $mg{\cdot}L^{-1}$ 2,4-D. Under the condition, the callus were efficiently induced and proliferated with comparably lower frequencies of callus browning. In shoot regeneration, the treatment of NAA combined with BAP seemed to contribute more efficient conditions to shoot regeneration than those of NAA with Kinetin or 2-iP. Especially, regeneration efficiency and number of regenerated plants were 83.7% and 5.5 in 3 $mg{\cdot}L^{-1}$ NAA with 5 $mg{\cdot}L^{-1}$ BAP, respectively, which were higher frequencies than those in NAA with Kinetin or 2-iP. In results, 5 $mg{\cdot}L^{-1}$ 2,4-D and 3 $mg{\cdot}L^{-1}$ NAA combined with 5 $mg{\cdot}L^{-1}$ BAP were efficient for embryogenic callus induction and regeneration of Miscanthus. This system would be useful for mass-propagation and developing new cultivars via tissue culture of Miscanthus sinensis.

Dichloroacetate Inhibits the Proliferation of a Human Anaplastic Thyroid Cancer Cell Line via a p53-independent Pathway (Dichloroacetate의 p53 비의존적 경로를 통한 인간 역분화 갑상선 암세포주의 성장억제 효과)

  • KC, Yam Bahadur;Poudel, Sunil;Jeon, Eon Ju;Shon, Ho Sang;Byun, Sung June;Jeoung, Nam Ho
    • Journal of Life Science
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    • v.28 no.12
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    • pp.1469-1476
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    • 2018
  • Occurrence of the Warburg effect in solid tumors causes resistance to cancer chemotherapy, and targeting energy metabolisms such as aerobic glycolysis is a potential strategy for alternative treatment. Dichloroacetate (DCA), an inhibitor of pyruvate dehydrogenase kinase (PDK), shifts glucose metabolism from aerobic glycolysis to oxidative phosphorylation (OxPhos) in many cancers. In this study, we investigated the anticancer effect of DCA on a human anaplastic thyroid cancer (ATC) cell line, 8505C. We found that DCA selectively inhibits cell proliferation of the 8505C line but not of a normal thyroid line. In 8505C, the cell cycle was arrested at the G1/S phase with DCA treatment as a result of decreased antiapoptotic proteins such as $HIF1{\alpha}$, PDK1, and Bcl-2 and increased proapoptotic proteins such as Bax and p21. DCA treatment enhanced the production of reactive oxygen species which consequently induced cell cycle arrest and apoptosis. Interestingly, DCA treatment not only reduced lactate production but also increased the expression of sodium-iodine symporter, indicating that it restores the OxPhos of glucose metabolism and the iodine metabolism of the ATC. Taken together, our findings suggest that PDK inhibitors such as DCA could be useful anticancer drugs for the treatment of ATC and may also be helpful in combination with chemotherapy and radiotherapy.

Optimization of Tyrosinase Production using Neurospora crassa (Neurospora crassa를 이용한 Tyrosinase 생산의 최적화)

  • 채희정;유영제
    • Microbiology and Biotechnology Letters
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    • v.19 no.3
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    • pp.281-289
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    • 1991
  • Neurospora crassa (KCTC 6079) produces tyrosinase (EC 1.14.18.1) during sexual differentiation under derepressed conditions in the presence of inducers such as amino acid analogues, antimetabolites or protein synthesis inhibitors. The selection of inducer concentration and induction time as well as inducer type are critical for the optimization of the enzyme production. The best inducer was found to be cycloheximide. Since cycloheximide was toxic to the cells, an optimal inducer concentration and an optimal induction time were determined to maximize the enzyme production from batch cultures. Mathematical models for the cell growth and the enzyme production were proposed and used for process optimization. By optimizing the induction conditions, maximum tyrosinase productivity was increased significantly.

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저온 플라즈마 반응기에서의 수정충돌주파수를 이용한 실리콘 나노 입자 형성 모델링

  • Kim, Yeong-Seok;Kim, Dong-Bin;Kim, Hyeong-U;Kim, Tae-Seong
    • Proceedings of the Korean Vacuum Society Conference
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    • 2014.02a
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    • pp.217.1-217.1
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    • 2014
  • 반도체 및 디스플레이 산업은 많은 공정들에서 저온 플라즈마 반응을 이용한다. 특히 소자 제작을 위한 실리콘 박막의 증착은 저온 플라즈마 공정의 주요 공정이다. 하지만 실리콘 박막을 합성하는데 있어서 저온 플라즈마에서 형성되는 실리콘 나노 입자는, 오염입자로써 박막의 특성을 악화시켜 소자생산 수율을 악화시키는 주요 원인이 되고 있다. 따라서 플라즈마에서 입자 형성의 원인이 되는 화학반응 및 입자들의 성장 매커니즘에 대한 연구는, 1980년대 플라즈마 공정에서 입자 합성이 보고된 이래 공정의 최적화를 위해 꾸준히 연구되어왔다. 이러한 매커니즘의 연구들은, 플라즈마 화학반응에 의해 실리콘 입자 핵을 만들어 내는 과정과 입자들이 충돌에 의해 성장해가는 과정으로 나눠진다. 플라즈마 화학 반응 과정은 아레니우스 방정식에 의해 정의된 반응계수를 이용하여 플라즈마 내 전자와 이온, 중성 화학종들이 전자 온도와 전자 밀도, 챔버 온도 등에 의해 결정되는 현상을 모사한다. 또한 이 과정에서 실리콘을 포함하는 화학종들의 반응에 의해 핵이 생성 되가는 양상을 모사한다. 생성된 핵은 충돌에 의해 입자가 성장해 가는 과정의 가장 작은 입자로써 이용된다. 입자들이 성장해가는 과정은 입자들이 서로 충돌하면서 다양한 입경의 입자로 분화되어가는 현상을 모사한다. 이 과정에 의해 다양한 입경분포로 분화된 입자들은 플라즈마 내 전자에 의해 하전되며, 이러한 하전 양상은 입경에 따라 다른 분포를 보인다. 본 연구에서는 입자의 하전 분포를 고려하여, 입자들의 성장의 주요 원인인 입자간의 충돌을 대표하는 충돌주파수를 수정하는 방식을 채택하여 보다 정밀한 입자 성장 양상을 모델링하였다. Inductively coupled plasma (ICP) 타입의 저온 플라즈마 반응기에서 합성된 입자들을 Particle Beam Mass Spectrometer (PBMS)와 Scanning Electron Microscope (SEM)를 이용하여 입경분포를 측정한 데이터와 모델링에 의해 계산된 결과를 비교하여 본 모델의 유효성을 검증하였다. 검증을 위해 100~300 mtorr의 챔버 압력 조건과 100~350 W의 입력 전력 조건들을 달리하며 측정한 결과와 계산한 데이터를 조건별로 비교하였다.

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Sporulation-associated Products of the Bacillus species

  • 김현욱
    • Proceedings of the Korean Society for Applied Microbiology Conference
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    • 1975.07a
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    • pp.109.1-109
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    • 1975
  • The family Bacillaceae에 속하는 모든 미생물은 endospore를 형성하는 것이 그 특성이며 포자형성은 일련의 생화학적 반응은 물론 형태ㆍ구조적 변화를 수반하는 질서 정연한 원시적인 생물분화의 일종이다. 따라서 포자형성 발아에 관한 연구가 많이 이루어졌고 또 현재도 활발히 진행되고 있다. 산업미생물학도로서 흥미있는 점은 산업적으로 유용한 몇가지 생화학물질이 포자형성 과정과 특별히 관련되어 생산된다는 점이며 이중 몇 가지 효소와 항생물질 그리고 독소에 관하여 간략히 고찰해 보고자 한다.

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工業集積論考

  • ;Hyung, Kie Joo
    • Journal of the Korean Geographical Society
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    • v.11
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    • pp.17-26
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    • 1975
  • This study examined the flexibility strategies and the spatial division of labour on production system in the industrial restructuring process of textile and apparel industry in Daegu. Textile and apparel firms in Daegu have come to have power after holding trade function. In the context of existing variant production units across all the sectors of the textile and apparel industry, individual firms that hold trade function can organize the lowest cost production system. Daegu's Textile and apparel industry in Daegu shows the complicated and closely realted production system through individual firm's flexibility strategies. Although the textile and apparel firms produce the same item or have the same size, they pursue different flexibility strategies and form different networked production system and different spatial division of labour.

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Economic Growth and Renewable Resource: Specialization of Clean Activities

  • Hwang, Sanghyun
    • Environmental and Resource Economics Review
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    • v.21 no.3
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    • pp.627-681
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    • 2012
  • This paper starts with a model of monopolistic competition and endogenous growth, and it adds pollution as an input to production. Then I adopt environmental quality as a renewable resource used in production. I show that increasing returns due to specialization of clean activities as inputs can help lead to sustainable growth with no harm to environmental quality. I also compare and evaluate alternative policy combinations (i.e. taxes +subsidies) that correct two distortions from pollution and monopolistic competition. Finally, I find that, if the productivity of environment in final good production is not sufficiently enough, the number of clean goods tends to increase with more environmental concerns.

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In Vitro Culture Following Purfication of Mouse Spermatogenic Cells (생쥐 정자세포의 분리와 체외 배양에 관한 연구)

  • 김묘경;김진회;이상민;정/하해숙;이훈택;정길생
    • Korean Journal of Animal Reproduction
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    • v.20 no.1
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    • pp.43-52
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    • 1996
  • This study was carried out to establish the in vitro short-term culture system of developing male germ cells by purifing germ cells of various stages. The decapulated testicular cells were incubated with collagenase (lmg/ml) and try psin (2.5mg/ml) in HBSS. After separating male germ cell, the separated germ cells were stained with heamatoxylin/eosin and determined developing stages under light microscopy. The purity of pachtene spermatocytes a and round spermatid were 85%, respectively. Yield of total male germ cells was highly variable between individuals, with a mean value of 3.5 to 4.5 ${\times}$ 10$^7$ cells/testis. Viability of the cell was over 97% after separation. In DMEM medium, the optimal cell number for culture is approximately 1 x 10$^5$ cells/dish, but low cell den-sities than 1 ${\times}$ 10$^5$ cell/dish showed a decreased cell viability. Furthermore, about :36.8% of pac-hytene cells was successfully cultured for 6 days and some of cells were developed to secondary spermatids and round spermatids. Therefore, our data suggested that this culture conditions will be utilize as a feasible tools to produce tran-sgenic livestock using techniques such as intrac-ytoplasmic injection and cell fusion.

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글로벌생산체계에서 중국 동관(東莞)지역의 역할과 IT·전자산업클러스터의 발전

  • Lee, Sang-Bin
    • Korea and Global Affairs
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    • v.1 no.1
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    • pp.153-176
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    • 2017
  • 중국 경제성장에 핵심적 역할을 해왔던 광동성(廣東省) 동관시(東?市)는 지리적으로 근접해 있는 홍콩을 이용하여 줄곧 경제발전을 추구해 왔다. 1980년대 초반은 홍콩으로부터 가전산업 관련 중소 다국적 기업이 동관지역에 진출하여 생산 공장을 세우고, 그 후 1990년 중반에는 대만의 PC제조업체의 진출이 잇따르면서 현재 동관에는 정보통신산업과 PC부품 및 전자산업 중심으로 지역 산업클러스터가 발전해 왔다. 동관시 산업클러스터 사례는 개혁개방이후 중국 제조업 산업의 발전뿐만 아니라, 글로벌 시대에 해외직접투자와 아시아지역 후-후발산업국가에서의 지역경제의 성장 및 산업발전과정의 연구에 대한 이론적 시사점을 줄 수 있는 지역적 사례 중의 하나다. 본 연구는 광동성 동관시 지역산업클러스터의 사례를 중심으로 이론적으로는 지역산업발전의 내생적 성장요인 분석(NMID) 혹은 글로벌 생산네트워크와의 외부적 연계성을 기초로 하는 산업의 지역적 분화(GPN)에 대한 이 두 관점을 상호 비교하고, 글로벌 생산체계에서 공간적으로 현재 동관시 지역이 가지고 있는 산업클러스터의 위치와 역할을 설명하였다. 또한 산업적인 측면에서 생산네트워크 접근틀과 제도적 역량이라는 이론적 관점을 활용하여 중국 동관시 지역산업과 기업들이 보유하고 있는 상대적 경쟁력을 분석하였다.