• Korean Journal of Organic Agriculture
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• v.13 no.2
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• pp.161-173
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• 2005

This study was carried out to clarify the effects of antifungal bacterial strains isolated from the greenhouse soil grown strawberry plants on the growth inhibition of plant pathogen, gray mold (Botrytis cinerea) infected in strawberry plants in Damyang and Jangheung districts. Antagonistic bacterial strains were isolated and investigated into the antagonistic activity against gray mold. Screened ten bacterial strains which strongly inhibited Botrytis cinerea were isolated from the greenhouse grown strawberry plants, and the best antifungal microorganism designated as SB 143 was finally selected. Antifungal bacterial strain SB 143 was identified to be the genus Bacillus sp. based on the morphological and biochemical characterization. Bacillus sp. SB 143 showed 59.4% of antifungal activity against Botrytis cinerea. By the bacterialization of culture broth and heated filtrates of culture broth, Bacillus sp. SB 143 showed 93.1% and 32.1% of antagonistic activity against Botrytis cinerea.

• Proceedings of the Korean Institute of Surface Engineering Conference
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• 2002.05a
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• pp.50-50
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• 2002

탄소 박막은 내마모, 화학적 생물학적 적합성 둥의 특성으로 언하여 많은 연구자들레 의하 여 연구되어지고 있다. 그러나 탄소 박막의 경우 높은 잔류응력과 이에 따른 낮은 밀착력으 로 언하여 증착 두께에 제한이 되어왔다. EB-PVD법을 이용하여 탄소막을 증착하는 경우 이러한 문제점을 해결하기 위하여 탄소막에 제 2의 금속원소를 첨가하거나 화합물 형태의 증착원 이용, 복합공정 (Hybrid process)을 통하여 증착 두께 의 제 한을 극복하고자 하는 연 구가 계속되고 있다. 본 연구에서는 탄소막의 잔류응력 제어와 증착 두께를 높이기 위하여 Plasma Activated E E-Beam Evaporation을 통하여 탄소막을 증착하였다. 탄소막 증착시 잔류응려과 밀착력에 대한 플라즈마의 영향올 알아보기 위하여 RF 플라즈마의 인업 전력에 따라 실험을 수행하였으며 필라멘트 전자 방출원을 이용하여 플라즈마 밀도가 미치는 영향도 알아보았다.

• Proceedings of the Korean Institute of Intelligent Systems Conference
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• 1998.03a
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• pp.16-19
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• 1998

본 논문에서는 생물학적인 DNA와 인위적인 DNA의 개발 메커니즘에 근거를 둔 새로운 DNA coding mithod를 소개한다. 이 방법은 DNA 유전자의 Redundancy 성질과 Over-lapping 성질을 분석하여 구성한 알고리즘으로, DNA 자체의 특성 때문에 염색체의 길이를 자유자재로 변화시킬 수 있는 장점을 가지고 있다. 즉 삽입과 삭제의 과정을 쉽게 처리할 수 있다. 따라서 본 연구에서는 이 DNA coding method를 사용하여 시스템 제어에 필요한 지식을 표현하는 적당한 퍼지규칙을, 전건부와 후건부의 구조 및 매개변수 동조를 통하여, 획득하고, 이 규칙을 새로운 유전자 알고리즘을 이용하여 최적화 시킬 수 있는 새로운 퍼지규칙 획득 알고리즘을 개발한다. 이 방법의 유용성을 입증하기 위하여 물탱크 시스템에 적용한 결과 효과적으로 퍼지규칙을 획득한다.

• Journal of Environmental Science International
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• v.21 no.3
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• pp.383-390
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• 2012

For the cost-effective biological nitrogen removal (BNR) process whose characteristics of influent have low COD/N ratios, the automatic control system for the addition of external carbon based on oxidation-reduction potential (ORP) data in an anoxic reactor has been developed. In this study, it was carried out with a pilot-scale Bardenpho process which was consisted of anoxic 1, aerobic 1, aerobic 2, anoxic 2, aerobic 3 tank and clarifier. Firstly, the correlation coefficient ($R^2$) of the dosage of external carbon source and ORP value was about 0.97. Consequently, the automatic control system using ORP showed that the dosage of external carbon source was decreased by about 20% compared with a stable dosage of 75 mg/L based on the COD/N ratio of the anoxic influent.

• Journal of Life Science
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• v.2 no.1
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• pp.2-10
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• 1992

cAMP-CRP complex에 의해 전사조절을 받는 관련유전자의 구조, 특징, 조절양상, 기작과 CRP의 생화학적 및 분자생물학적이해 등을 살폈다. 그러나 대장균의 전체유전자의 구조해석이 연구사업으로 실행되어지고 있는 현실에서 볼 때 CRP의 조절하에 있는 유전자군이 보다 더 복잡 다양한 system으로 생각되어져서 CRP조절계의 전체적인 양상으로 해명하기 위한 연구도 이루어질 것으로 생각되어진다. 즉, cAMP-CRP의 조절하에 있는 조절영역의 전체를 파악함은 operon의 발현조절의 해명에 중요한 기틀이 될 뿐만 아니라 개별유전자의 이해를 위한 것보다 복잡한 세포 전체의 제어계를 분자적수준에서 해결하는데 중요한 과제라고 사료되어진다. 몇 가지 연구 결과에서는 cAMP-CRP가 결합은 하면서도 발현조절에는 관여하지 않는다든지, cAMP가 결합하지 않으면서도 전사조절을 받을 수 있는 유전자들이 밝혀지고 있다.

• Journal of Wetlands Research
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• v.25 no.3
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• pp.184-195
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• 2023

To develop an effective management plan for Ambrosia trifida L., an invasive alien plant in Korea, we assessed the potential of Ophraella communa LeSage as a biological control agent. This involved investigating the host specificity of the herbivore Ophraella communa LeSage, its annual travel distance, and the impact of this insect on the fitness of Ambrosia trifida L. We confirmed the host plant preference of Ophraella communa LeSage. The travel distance of this insect was determined by monitoring its appearance in selected Ambrosia trifida L. communities without these insects at distances of 10, 20, 30, and 100 meters, based on the locations where the presence of Ophraella communa LeSage was observed. The growth, reproductive, and physiological responses of Ambrosia trifida L. were measured according to feeding by Ophraella communa LeSage. As a result, Ophraella communa LeSage fed on only three taxa and moved short distances within a radius of 30 m per year from the host. The feeding behavior of the herbivore had a negative impact on the growth, reproductive, and physiological responses of Ambrosia trifida L. And the plant's growth and reproduction improved with increasing distance from the herbivore. Furthermore, the introduction of herbivores was able to control over 90% of Ambrosia trifida L. when the coverage of the Ambrosia trifida L. group was below 50%. However, the effectiveness of the removal decreased when the coverage exceeded 90%. These results are likely to be utilized by Ophraella communa LeSage as an ecological control agent. It is advantageous to introduce them in spring (May) when the coverage is low to maximize the effectiveness of control.

• Journal of Animal Science and Technology
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• v.46 no.2
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• pp.251-260
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• 2004

The feasibility and stability of ORP, pH(mV) and DO as a real-time control parameter for SBR process were evaluated in this study. During operation, NBP(nitrogen break point) and NKP(nitrate knee point), which reveal the biological and chemical changes of pollutants, were clearly observed on ORP and pH(mV)-time profiles, and those control points were easily detected by tracking the moving slope changes(MSC). However, when balance of aeration rate to loading rate, or to OUR(oxygen uptake rate), was not optimally maintained, either false NBP was occurred on ORP and DO curves before the appearance of real NBP or specific NBP feature was disappeared on ORP curve. Under that condition, however, very distinct NBP was found on pH(mV)-time profile, and stable detection of that point was feasible by tracking MSC. These results might mean that pH(mV) is superior real-time control parameter for aerobic process than ORP and DO. Meanwhile, as a real-time control parameter for anoxic process, ORP was very stable and more useful parameter than others. Based on these results, a stable real-time control of process can be achieved by using the ORP and pH(mv) parameters in combination rather than using separately. A complete removal of pollutants could be always ensured with this real-time control technology, despite the variations of wastewater and operation condition, as well as an optimization of treatment time and capacity could be feasible.

• Korean Journal of Environmental Biology
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• v.20 no.3
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• pp.256-262
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• 2002

This research investigated experimentally on the population growth in the aquatic microcosm with the wastewater of plating factory. The purpose of this study was to evaluate the effect of culture conditions of the characteristic growth pattern of the examined species. Population of the system is consists of three organisms; Chlorella vulgaris as a producer, Cyclidium glaucoma as a consumer and Pseudomonas putida as a decomposer. The different growth patterns of each population are followed by surfactant type; Especially C. glaucoma was sensitive, Ch. uvlgaris was maintained population size stably even at high level of surfactant and p. putida was not significantly affected. After treatment of waste water from plating factory, it began to be affected at 1.0% solution treatment to Ch. vulgaris which the cell number was decreased prominently after 2 days, and C. glaucoma was disappeared at 2.5% solution treatment. P. putida was showed increasing pattern according to treatment concentration, at 2.5% solution and population size grew double. The result from current microcosm study indicates that this model system can be applied to environmental assessment method for various pollutants.

• The Korean Journal of Mycology
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• v.47 no.4
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• pp.417-425
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• 2019

Biocontrol agents (BCAs) are widely used to protect plants from diverse biotic and abiotic stresses in agricultural and ecological fields. Among the various microbes, many subspecies of the gram-positive genus, Bacillus, have been successfully industrialized as eco-friendly biological pesticides and fertilizers. In the current study, we demonstrated that Bacillus thuringiensis C25 exhibited antagonistic effects on the mycelial growth of Rosellinia necatrix, a fungal phytopathogen. Scanning electron microscopy analysis revealed that B. thuringiensis C25 degraded the cell wall structures of R. necatrix mycelia. In the functional genomic analysis of B. thuringiensis C25, we annotated 5,683 genes and selected the gene sets that potentially encoded fungal cell wall degrading enzymes (CWDEs). The growth inhibition effects on R. necatrix were highly correlated with the transcriptional activity of the mycelial cell wall degrading genes of B. thuringiensis C25. The transcript levels of CWDEs, including CshiA, B, and Glycos_transf_2 genes in B. thuringiensis C25, were enhanced following co-cultivation with R. necatrix. In conclusion, our study suggested that B. thuringiensis C25 could serve as a suitable candidate for controlling R. necatrix and could facilitate elucidating the mechanisms underlying the antifungal activities of BCAs against phytopathogens.

• The Sea:JOURNAL OF THE KOREAN SOCIETY OF OCEANOGRAPHY
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• v.12 no.4
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• pp.359-369
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• 2007

Amoebophrya is an obligate endoparasitic eukaryotic dinoflagellate infecting host species and eventually killing them within a short period. Because of its host specificity and significant impacts on population dynamics of host species, it has long been proposed to be a potential biological agent for controlling harmful algal bloom (HAB). For several decades, the difficulties of culturing host - parasite systems have been a great obstacle to further research on the biology of Amoebophrya but recent success of several culture systems reactivates this research field. In this study, as a preliminary work for understanding the impacts of Amoebophrya on the population dynamics of host species, semimonthly occurrence of infected host dinoflagellates by Amoebophrya spp. had been observed in Jinhae Bay for two years and with a host - parasite system cultivated, host specificity of Amoebophrya spp. on several dinoflagellates was tested. Amoebophrya spp. were observed in the cellular organelle and cytoplasm of several species including Akashiwo sanguinea, Ceratium fusus, Dinophysis acuminata, Heterocapsa triquetra, Oblea sp., Prorocentrum minimum, P. triestinum, Scrippsiella spinifera, and S. trochoidea. Among them two host - parasite systems for an athecate dinoflagellate, A. sanguinea, and for a thecate dinoflagellate, H. triquetra, had been able to be successfully established as laboratary cultures. Cross-infection tests for 6 species of dinoflagellates in which Amoebophrya was observed or had been reported to exist confirmed high preference for host species of the parasite. Through the continuous research on Amoebophrya occurring in Korean coastal waters, we need to maintain various host - parasite culture systems, which will be very helpful for understanding its ecological role in marine food webs and for applying the species to biologically control harmful algal blooms.