• Title/Summary/Keyword: 생물학적방제

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The suggestion for mosquito control methods on mosquito habitat by land cover map classification (토지피복도 분류체계와 연계한 모기 서식지와 적용가능 방제법 검토)

  • Kim, Jeong-Eun;Lee, Hwang-Goo;Kim, Dong-Gun
    • Korean Journal of Environmental Biology
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    • v.38 no.1
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    • pp.189-196
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    • 2020
  • Mosquitos serve as vectors for diseases, causing inconvenience as well as a threat to human life and health. Concern about mosquitos introducing and spreading new diseases has been intensifying. We observed a variety of mosquito habitats based on land cover classification from Korea's Ministry of Environment, and the mosquito species that could appear were classified according to the each habitat type. Finally, we suggested the best control methods for each type of habitat considering habitat characteristics and the ecological traits of mosquitos. Urban areas harbor various habitats for pests, contributing significantly to mosquito habitats. Control must be performed regarding larva and adults because various sources for habitats exist. Public mosquito control programs such as educational training, as well as information brochures can be effective in managing mosquito populations and public health. Agricultural areas show high densities of mosquito larva to lentic zones such as reservoirs, wetlands, paddy fields. So, biological control using natural predators may be effective in controlling mosquito populations. Forests are major habitats for Aedes albopctus, so physical controls should be deployed for residents living nearby, and excessive deforestation should be minimized. Other areas including aquatic ecosystems should be adopted regarding biological control using Bti (Bacillus thuringiensis var. israelensis) and chemical control for eradicating mosquitos. We classified habitats into four types of land cover patterns considering ecological traits and habitat preference, and suggest adequate control methods for each habitat type. Our suggestion can be used to positively contribute toward effective managing mosquito's density and reducing the damage to public health.

Control of Ginseng Damping-off by Streptomyces sp. A3265 (방선균 A3265 균주에 의한 인삼 잘록병의 방제)

  • Woo, E-Eum;Lee, Gang-Seon;Lee, In-Kyoung;Choi, Jae-Eul;Yun, Bong-Sik
    • The Korean Journal of Mycology
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    • v.44 no.3
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    • pp.193-195
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    • 2016
  • Korean ginseng (Panax ginseng) possesses various biological and pharmacological properties. Damping-off is a critical disease on ginseng seedlings, which is caused by the fungal pathogens Rhizoctonia solani and Pythium sp.. This disease is generally controlled by the application of fungicides, but also biological control is an efficient and environmentally friendly way to prevent ginseng damping-off. In a previous study, we screened soil-borne bacteria with potential applications as biological control agents for ginseng damping-off and selected the bacterial strain Streptomyces sp. A3265, producing antifungal substances guanidylfungin and methylguanidylfungin. In this study, we investigated control efficacy of Streptomyces sp. A3265 against ginseng damping-off in the field. As a result, the incidence of damping-off was significantly reduced when soaking ginseng seeds in the culture broth of Streptomyces sp. A3265.

Screening of Antagonistic Bacteria having Antifungal Activity against Various Phytopathogens (다양한 식물병원성 곰팡이에 항진균 활성을 갖는 길항미생물의 탐색)

  • Yang, Hee-Jong;Jeong, Su-Ji;Jeong, Seong-Yeop;Jeong, Do-Youn
    • The Korean Journal of Mycology
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    • v.42 no.4
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    • pp.333-340
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    • 2014
  • The aim of this study was to isolate a potential multifunctional biocontrol agent from bacteria for control of multiple plant diseases as an alternative to fungicides. A total of 201 strains were isolated from soil undamaged by repeated cultivation in Sunchang and their ability to produce antibiotics, siderophores and extracellular enzymes such as protease, cellulase and amylase was investigated. Selected strain SCS3 produced cellulose, protease and amylase. This strain also produced siderophores and showed excellent antifungal activity against various phytopathogens. SCS3 was identified as Bacillus subtilis using 16S rRNA sequencing, and named Bacillus subtilis SCS3. Finally, physiological and biochemical characteristics of B. subtilis SCS3 were examined. From the results, B. subtilis SCS3 was found to be a useful multifunctional biocontrol agent against various phytopathogens.

Biocontrol Activity of Myxococcus sp. KYC 1126 against Phytophthora Blight on Hot Pepper (점액세균 Myxococcus sp. KYC 1126을 이용한 고추 역병 생물학적 방제 효능)

  • Kim, Sung-Taek;Yun, Sung-Chul
    • Research in Plant Disease
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    • v.17 no.2
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    • pp.121-128
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    • 2011
  • Bacteriolytic myxobacteria have been known to secrete various antifungal metabolites against several soilborne phytopathogens including Phytophthora. Among the three isolates of Myxococcus spp., KYC 1126 and KYC 1136 perfectly inhibited the mycelial growth of Phytophtora capsici in vitro. In order to show the biocontrol activity on Phytophthora blight of hot pepper, we tried to find the best way of application of a myxobacterial isolate. Although KYC 1126 fruiting body was easily grown on the colony of Escherichia coli as a nutrient source, it did not control the disease when it was pre-applied in soil. Before the bioassay of a liquid culture filtrate of KYC 1126 was conducted, its antifungal activity was confirmed on the seedlings applying with the mixture of the pathogen's zoospore suspension and KYC 1126 filtrate. On greenhouse experiments with five and four replications, the control value of KYC 1126 on phyllosphere and rhizosphere was 88% and 36%, respectively. Whereas, the control value of dimetnomorph+propineb on phyllosphere was 100% and that of propamorcarb on rhizosphere was 44%. There was a phytotoxicity of the myxobacterial filtrate when seedlings were washed and soaked for 24 hours. Gummy materials were covered with roots. And stem and petiole were constricted, then a whole seedling was eventually blighted.

Biocontrol of Cabbage Clubroot by the Organic Fertilizer Using Streptomyces sp. AC-3. (Streptomyces sp. AC-3을 이용한 배추 무사마귀병의 생물학적 방제)

  • 주길재;김영목;김정웅;김원찬;이인구;최용화;김진호
    • Microbiology and Biotechnology Letters
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    • v.32 no.2
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    • pp.172-178
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    • 2004
  • This research is performed for a biological control of Chinese cabbage clubroot, we isolated an antagonistic bacterium AC-3 against Plasmodiophora sp., causal pathogens of cabbage clubroot. The isolated strain was identified as Streptomyces sp. by culture morphology, biochemical reactions, and homology research based on l6S rDNA sequences. Streptomyces sp. AC-3 produced chitinase (9.3 units/$m\ell$) in culture broth. So Plasmodiophora sp. mycelia changed abnonnal swelling, curling and branching mycelia by Streptomyces sp. AC-3 culture. In a field infected by Plasmodiophora sp., the treatment of a organic fertilizer added 2% Streptomyces sp. AC-3 microbial inoculant, it resulted in about 50% reducing the severity of cabbage clubroot significantly on cabbage plants compared with treated organic fertilizer plants. Additional disease such as sclerotinia rot, fusarium wilt and pythium rot were also significantly reduced by the treatment of the organic fertilizer added Streptomyces sp. AC-3 microbial inoculant.

Biological Control of Root-Lesion Nematodes(Pratylenchus spp.) by Nematode-Trapping Fungi (선충 포식성 곰팡이를 이용한 뿌리썩이선충(Pratylenchus spp.)의 생물학적 방제)

  • 손흥대;김성렬;최광호;추호렬
    • Journal of Life Science
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    • v.10 no.4
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    • pp.403-407
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    • 2000
  • For the biological control of the root-lesion nematodes, Pratylenchus spp., which damage directly and indirectly to the leaf perilla, the nematical effect of three nematode-trapping fungi, Arthrobotrys oligospora, A. conoides and A. dactyloides was evaluated in the field. Three species of Arthrobotrys were isolated from the culture soil of leaf perilla in 1998 and were observed the capture of the root-lesion nematodes, Pratylenchus spp. by adhesive hyphal networks or constricting rings on agar. At 40 days after treatment, the plant-parasitic nematodes and root-lesion nematode populations were approximately increased 3.5 fold in untreated control plot, while the nematode population in fungi treatment plots was similar to initial population. In the A. dactyloides plot, however, the population of plant-parasitic nematodes and Pratylenchus spp. was approximately reduced 65% and 53%, respectively. Thus, the fungus A. dachyloides should provide as biological agent for the control of Pratylenchus spp.

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