• Proceedings of the Korean Society of Fisheries Technology Conference
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• 2000.05a
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• pp.503-504
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• 2000

바지락포자충 Perkinsus는 미국과 유럽 등지에서 굴과 바지락에 기생하며 고수온기 숙주의 대량 폐사를 유발하는 원생동물로 이에 대한 연구가 활발하게 진행중인 패류질병이다. Choi 와 Park (1997)은 1990년대 초반부터 나타난 여름철 바지락 대량 폐사 원인 중의 하나로 바지락포자충을 폐사 원인 생물로 추정한 바 있다. 이 연구는 1997년부터 진행중인 우리 나라 전 해역에 대한 바지락포자충 감염현장 조사의 일환으로, 경남지역에 분포하는 바지락의 바지락포자충 감염에 대한 조사 보고이다. (중략)

• Korean Aquaculture
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• v.14 no.2
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• pp.49-51
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• 2002

• Proceedings of the Korean Aquaculture Society Conference
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• 2003.10a
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• pp.99-99
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• 2003

1. 수온과 염분에 따른 부유유생의 성장과 생존율. 유생의 수온별 사육실험 결과, 3$0^{\circ}C$에서는 실험시작 6일 후 모두 폐사하였으며, 18$^{\circ}C$에서는 높은 생존율에 비하여 늦은 성장을 보였다. 24$^{\circ}C$와 27$^{\circ}C$에서 빠른 성장을 보였으나, 27$^{\circ}C$에서는 성장에 비하여 낮은 생존율을 보였다. 염분에 따른 유생의 사육시험 결과 0$\textperthousand$에서는 실험시작 10일 후 모두 폐사 하였으며, 성장은 6~9$\textperthousand$, 생존율은 3$\textperthousand$에서 가장 높게 나타났다. 2. 수용밀도에 따른 부유유생의 성장과 생존율. 유생의 수용밀도에 따른 사육실험 결과, 1$m\ell$당 1~5 개체의 저밀도에서는 성장과 생존율이 양호하였으며, 50개체/$m\ell$의 고밀도에서는 성장과 생존율이 낮았으며, 실험시작 12일째에 모두 폐사하였다. 3. 먹이생물 종류 및 공급량에 따른 부유유생의 성장과 생존율. 식물 먹이생물에 따른 유생사육 실험 결과, 혼합 공급구가 가장 빠른 성장을 보였으며, 단일 공급구에서는 I. galbana, P. lutheri, C. calcitrans 순으로 비슷한 성장을 보였으며, Chlorella sp.는 가장 늦은 성장을 보였다. 생존율 또한 혼합구가 가장 높고 I. galbana, P. lutheri, 가 비슷한 반면, Chlorella sp. 실험구가 가장 낮았다. 공급량에 따른 사육 실험에서는 먹이 공급량이 많을수록 유생의 성장은 양호하였으나, 8일 이후 50,000 cell/$m\ell$ 밀도에서 전량 폐사하였다. 실험 종료시인 12일째에는 20,000 cell/$m\ell$ 밀도에서 가장 빠른 성장과, 10,000 cell/$m\ell$ 밀도에서 높은 생존율을 보였으며, 5,000 cell/$m\ell$ 밀도에서는 성장과 생존율이 낮았다. 따라서 유생사육을 위한 기수재첩의 먹이생물의 밀도는 10,000~20,000 cell/$m\ell$가 효과적이었다.

• Journal of agriculture & life science
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• v.53 no.1
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• pp.117-126
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• 2019

This paper presents an experiment to examine the possibility of treating carcass while making compost with a horizontal cylinder composting equipment. The findings were as follows: as for heavy metal content, zinc content was above the allowed level in some measuring sections of the general operation and the entire measuring sections of the carcass operation, whose copper content was higher than the general operation. The wood chip operation was lower than the allowed level in all the heavy metals and similar or the same as the carcass operation in copper, cadmium and arsenic. Its nickel content was 29.5~63.8%, which was relatively higher than 9.3~18.0% and 15.8~18.0% of the general and carcass operation, respectively. Its chrome content was 14.2~31.9%, which was relatively higher than that of the general and carcass operation. The integrated operation was lower than the allowed level in all the heavy metals. Its copper and zinc content was 34.9~54.5% and 53.1~75.9%, which was similar to 48.9~52.6% and 64.6~85.9%, respectively, in the wood chip operation. Its chrome and nickel content was stabilized while their content was rather high in the wood chip operation. The average and final moisture content of the carcass operation was 60.7% and 49.6%, respectively. Its average moisture content was a little bit higher than the recommended level of 55.0%, but its final moisture content was lower than the recommended level. The average and final moisture content of the integrated operation was 29.2% and 18.6%, respectively, which was the lowest level among the four operation modes. The overall moisture content of the operation modes was under the recommended level of 55.0% with some variance among the modes. The C/N ratio was the lowest in the carcass operation and the highest in the general operation. The average C/N ratio of the operation modes was in the range of 13.7~20.3 with the total average of 18.3, and the modes kept it under the proper level of 30.0.

• Proceedings of KOSOMES biannual meeting
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• 2007.05a
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• pp.97-99
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• 2007

대기중 이산화탄소 농도상승을 억제하기 위하여 $CO_2$를 해양에 처리하는 방안이 제안되고 있으며 해양생태계 및 해양생물에 대한 생물학적 영향에 대한 검토가 신중하게 검토되어야 할 것으로 생각된다. 본 연구에서는 고농도의 이산화탄소 환경에서 천해어종인 방어 및 넙치의 심혈관계 반용을 비교 검토하였다. 5% $CO_2$ 환경에서 방어는 8시간 이내에, 넙치는 48 시간 이내에 100% 폐사하였으며, 넙치는 혈액 pH가 회복하였음에도 불구하고 폐사하는 경향이었다. 방어의 cardiac output (심박출량)은 stroke volume (박동량)의 변화에 의해서 영향을 받으며, 넙치는 heart rate (심박수)의 변화가 심박출량의 변화에 큰 영향을 끼치는 것으로 나타났다.

• Journal of Life Science
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• v.31 no.6
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• pp.574-580
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• 2021

This study investigated the pathogenicity of different genotypes of infectious hematopoietic necrosis virus (IHNV) strains isolated from infected rainbow trout in Gangwon Province. RtPc0314c and RtPc0314g strains belonging to the JRt-Shizuoka lineage and RtPc0816g strain belonging to the JRt-Nagano lineage showed 100% cumulative mortality when inoculated at a high titer. In addition, more rapid necrosis was observed in rainbow trout infected with RtPc0314c and RtPc0314g mutations. When inoculated at a low titer, 100% mortality was not observed until the end of the experiment, but the mortality was higher in rainbow trout infected with a mutant strain belonging to the JRt-Shizuoka linage than a mutant strain belonging to the JRt-Nagano lineage. A histopathological analysis showed a clear signature of infection in kidney and spleen tissues upon infection with RtPc0314c and RtPc0314g but no signature of infection associated with the Rt03186 strain. Based on the results in this study, it seems that strains belonging to the JRt-Shizuoka lineage in Gangwon Province IHNV are more pathogenic.

• Korean Journal of Environmental Biology
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• v.31 no.4
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• pp.393-401
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• 2013

The objectives of this study were to analyze structures of fish community and the ecological health using a multi-metric fish model, the Index of Biological Integrity (IBI) in the Backje Weir of Geum River during two periods namely before-the-fishkill ($B_f$) and after-the-fishkill ($A_f$). The total number of fish species observed were 32 and among them 10 species (35%) were Korean endemic species. The exotic species observed were 3 which decreased by 0.4% after-the-fishkill ($A_f$). The dominant species were Opsariichthys uncirostris amurensis (13%) at the Bf period and Squalidus japonicus coreanus (17%) at the Af period in the Backje Weir. At after the fishkill ($A_{f-I}$) total biomass was about 10 times lower than the biomass before-the-fishkill ($B_f$). The biomass of Carassius auratus decreased 98% after-the-fishkill and as time passed by the biomass recovered to nearly 100%. Similar decrease in the biomass occurred in the population of Opsariichthys uncirostris amurensis, while Rhinogobius brunneus population increased. According to the structure analysis of fish community, species richness index, evenness index and species diversity index were high but after-the fishkill, the values of indices decreased. Tolerant species (64%) dominated the fish community, and the sensitive species (2%) were rare, indicating the degradation of the ecosystem. According to analysis of the multi-metric model (IBI), the mean model value of the fish community in Backje Weir was estimated as 17.5 indicating a "fair condition".

• Korean Journal of Fisheries and Aquatic Sciences
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• v.24 no.1
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• pp.70-78
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• 1991

Both long period of summer drought and unusual high water temperature in 1988 caused the harmfull effect to the aquacultural organisms in south coast of Korea. One of those was the mass mortality of arkshell seedlings in Jinhae Bay. The purpose of this study was to verify the effects of environmental factors on arkshell seedlings by investigating not only water quality and food organisms in culturing grounds, but also meteorological conditions. Water temperature in November was higher at $2.0^{\circ}C$ than mean water temperature and the concentration of total inorganic nitrogen and N/P ratio were $0.38{\mu}g-at/l,$ 0.98, respectively which indicated the exhaustion of nutrients, especially nitrogen in the study area. In analysis of phytoplankton population structure, species diversity index(H) , equitability-index(e), species richness $index(d_1)$ were low, but dominance $index({\delta}_2)$ was high at the value of 98.4 and dominant species was Nitzschia pungens. In conclusion, it seems that mass .mortality of arkshell seedlings in November was resulted from the metabolism disorder with poor food organism under the unstable marine environment.

• Journal of fish pathology
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• v.14 no.2
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• pp.91-95
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• 2001

Mass mortalities occurred among red drum larvae, 20 to 30 days old, culturing at hatcheries on southern costal area. No specific external signs were observed except abnormal swimming and spinal deformity. It was, however, suspected as a viral etiology due to high mass mortalities so that histopathological and molecular biological study was performed to evaluate the agent. Both vacuoles and necrosis were observed on nerve cells of brain and eye by H-E staining, and viral particles were observed on electronmicroscopic examination. On the other hand, DNA fragment, approximately 426 bps, was amplified with RT-PCR. The above results were able to diagnosis the etiological agent of mass moralities in red drum larvae as VNN(viral nervous necrosis)virus.

• Proceedings of the Korean Environmental Sciences Society Conference
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• 2007.05a
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• pp.282-284
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• 2007

연구 해역의 양식 굴 폐사는 여름철을 지나 수온이 하강하기 시작하는 9월 초순에서 중순경부터 시작되었으며, 강진만 중앙부분 양식장의 경우 조사당시에도 상당수 굴의 폐사가 진행되고 있었다. 병리학적 조사 결과, 각 조사정점별 채집한 굴에서 비브리오 속 세균들이 검출되었으나, 이들 세균은 해수 중에 상존하는 종류로 알려저 있으며, 기생충은 검출되지 않았다. 강진만과 인근한 남해와 산청기상관측소에서 측정된 기온(8${\sim}$9월)은 남해는 2005년에 전반적으로 낮았으며, 산청은 전년비 $2{\sim}5^{\circ}C$ 높은 상태를 유지하였으며, 강수량은 강진만에 유입되는 담수의 대부분을 차지하는 남강댐 수위에 영향을 미칠 것으로 예상되는 산청지방의 2005년 여름철(7${\sim}$9월) 일별 강수량은 집중호우의 특성을 보이지 않았으며, 굴 폐사가 발생한 9월의 강수량은 남해와 산청지방에서 전년과 평년비 $99.9{\sim}188.0mm$나 적은 양을 보였다. 해양환경측정망(St. 2, 강진만) 자료에 2005년 하계 수온이 2004년에 비해 $2^{\circ}C$, 2003년에 비해 $2.6^{\circ}C$ 높은 값을 보였으며, 양식 굴의 먹이생물과 직접적으로 연관이 있는 Ch1-a 농도는 2005년에는 대단히 낮은 값(2.15ug/L)을 보였으며, SS 농도도 낮은 값(4.6mg/L)을 보였다. 조사당시 수질환경은 양식 굴의 폐사에 직접적인 영향을 미칠만한 요인은 관찰되지 않았으나, 조사정점 1(지족)의 저층에서 2.87mg/L의 낮은 용존산소가 관찰되었으며, 2005년 8월 15일 관찰된 강진만의 남-북방향 수직분포도 저층을 따라서 3mg/L이하의 빈산소 수괴가 관찰되었다.