• Journal of Korean Society of Environmental Engineers
• /
• v.22 no.10
• /
• pp.1833-1841
• /
• 2000

Microelectrode probe was made and applied to the biofilm in the biological treatment process as the state-of-art technology in order to actually measure the biofilm thickness, ionic concentration gradient, and material transport, etc. instead of classical theoretical approach. The working microelectrode, one of the main components of microelectrode probe, was easily contaminated and broken when determining the differences in the ionic concentrations through the measurement of biofilm's EMF (electromotive force). As a demonstration, two microelectrode probes were constructed in our lab for the measurement of the pH and $NO_3{^-}$ concentration in denitrifying biofilm. The microelectrode probe through the inner biofilm ($350{\mu}m$ from the surface of biofilm) showed that the pH was increased from pH 8 in the bulk solution to pH 8.3, on the other hand, the $NO_3{^-}$ concentration was decreased from 30 fig N/L in the bulk solution to 4 fig N/L.

• KSBB Journal
• /
• v.6 no.2
• /
• pp.115-121
• /
• 1991

A number of experiments were conducted in order to investigate the COD removal rate according to the biofilm thickness in a Fluidized-Bed Biofilm Reactor(FBBR). The following conditions were fixed during the experiments: superficial upflow velocity was 0.47cm/sec, operating temperature was $22{\pm}1{\circ}C$ and pH was about $7{\pm}0.1$. The synthetic wastewater based on glucose was used as a substrate. The COD removal efficiencies were shown as 73% and 95%, respectively, when organic loading rate was increased from $10kgCOD\;/\;{\textrm{m}^3}$.day to $80kgCOD\;/\;{\textrm{m}^3}$.day. Andrew's model of substrate removal rate which was commonly used in fixed-biofilm reactor was transformed and applied in this FBBR experiment to predict substrate removal rate and gave 85% agreement with the experimental values.

• Proceedings of the Membrane Society of Korea Conference
• /
• 1995.04a
• /
• pp.62-63
• /
• 1995

혐기성 소화는 고농도의 유기폐수를 최종적으로 메탄으로 전환하여 에너지원을 회수할 수 있는 효율적인 생물학적 폐수처리 공정 중의 하나이다. 그런데 이러한 혐기성 소화 공정에서 가장 큰 장애요인으로 작용하고 있는 요소 중의 하나가 매생물의 침강성 저하로 인한 고액분리의 문제이다. 이로인해 현재 고전적인 중력침강법, 부상법 대신에 분리막을 이용한 막결합형 혐기성 소화 공정이 대두되고 있으며 완전한 고액분리, 반응조내의 고농도 미생물 보유, 양질의 최종 유출수 획득 등 많은 장점들이 제시되고 있다. 그러나 이러한 장점에도 불구하고 시간에 따른 막오염 현상, 분리막 표면에서의 케이크층 형성 등으로 인한 막투과 유속 (flux)의 감소는 분리막의 응용에 있어 경제성을 저하시키는 주요한 부정적 요인으로 지적되고 있다. 그리고 막분리 성능은 분리막의 특성, 유체역학적 조건, 그리고 혐기성 소화 상태 등에 의해 영향을 받을 수 있다. 본 연구에서는 막결합형 혐기성 소화 공정의 십자흐름 (crossflow) 막분리 공정에서 유체역학적 조건 및 혐기성 소화조의 상태와 관련된 요인들이 어떻게 막투과도 및 배제율 등에 영향을 미치는 지 살펴보고자 한다.

• Proceedings of the Membrane Society of Korea Conference
• /
• 1997.10a
• /
• pp.133-134
• /
• 1997

1. 서론 : 제지산업은 용수 다소비용 산업으로, 생산하는 제품 톤당 약 40톤으로 폐수가 배출되고 있으며 공정내로 재활용하는 폐수량을 고려한다면 그 사용량은 더욱 클 것이다. 이와 같은 다량의 용수 사용으로 인하여 제지 공장의 폐수를 제조공정에서 재활용하는 방법이나 배출량을 감소시키는 방안들이 법률적이나 경제적인 측면에서 연구되고 있는 실정이다. 본 연구에서는 기존의 응집, 침전공정을 대신하고 여재를 이용힌 물리적 여과와 활성높은 미생물이 여재 표면에 부착되어 수중 유기물질을 생물학적으로 분해 제거하는 이중의 효과를 거둘 수 있는 호기성 생물막 공정을 도입하고 그 처리수의 유기물 및 용존 무기물을 Membrane 공정으로 제거함으로써 제지공업폐수를 재활용하여 용수기준까지 처리하 System의 개발을 그 목적으로 하였다.

• Proceedings of the Korean Environmental Sciences Society Conference
• /
• 1994.10a
• /
• pp.43-43
• /
• 1994

• Membrane Journal
• /
• v.8 no.2
• /
• pp.69-76
• /
• 1998

Membrane technologies have been used frequently in industries, taking advantage of that it is energy-saving and employable in relatively large scale. The fact that a non-mass separating agent is used in mild conditions without phase change in membrane separation makes it a method of choice in the recovery of biological materials. Recently, the development of noble separating modules has been solving the inherent problems in membrane separation, the fouling and the concentration polarization. In addition, membrane separation has broadened its applications from the conventional crude separation to the purificational use by the advent of the new and functional membrane materials. The role of membrane technologies is expected to be enormous in the production and recovery of biological products, considering the excellent applicability of membrane in the fields of integrated separation and in-situ separation, the two trends in modem bioseparation.

• Journal of the korean academy of Pediatric Dentistry
• /
• v.47 no.4
• /
• pp.406-415
• /
• 2020

Silver diamine fluoride (SDF) is an effective and efficient agent for arresting dental caries. It can be useful in treating children with behavioral or medical limitations. The purpose of this study was to evaluate the antimicrobial effect of SDF by using salivary biofilm. Pellicle-like saliva coated structure was prepared by using unstimulated saliva. For developing cariogenic biofilm, Streptococcus mutans was added to the mixture of pooled saliva and inoculated into a saliva coated glass or chamber. SDF was applied to cariogenic biofilm to evaluate the antimicrobial effect of SDF. As time passed, total bacteria and S. mutans were reduced after application of SDF (p < 0.000). Confocal laser scanning microscope also showed the increment of the ratio of dead cell. As a result of experiment using enamel and dentin of primary teeth, it was confirmed that the growth of cariogenic biofilm was inhibited when the SDF was treated (p = 0.029 each). This study showed excellent anti-microbial effect of SDF. And anti-caries effect in clinical practice can be expected.

• Transactions of the Korean Society of Mechanical Engineers B
• /
• v.36 no.11
• /
• pp.1119-1126
• /
• 2012

The collective behavior of bacteria plays an important role in biofilm development. In this study, the fluidic properties of biofilms formed in Escherichia coli (E. coli) and Bacillus subtilis (B. subtilis) colonies were compared by visualizing 200-nm fluorescent beads that were initially embedded in an agar plate and distributed spontaneously on the upper surface of the growing colonies. We conducted experiments to measure the three-dimensional profile of the E. coli colony using fluorescent microbeads that did not flow in the colony. Vortical flow patterns near the edge of the B. subtilis colony were observed clearly by tracking the movement of the beads in the biofilm of the colony. The present study should be the first step toward determining the effect of fluidic biofilms on the growth and swarming dynamics of bacteria.

• KSCE Journal of Civil and Environmental Engineering Research
• /
• v.11 no.1
• /
• pp.189-196
• /
• 1991

The objective of this study was to investigate the effect of temperature on microbial characteristics and treatment efficiency in aerated submerged biofilm process. From the results of the research, conclusions were derived as following: 1. Biofilm density, attached biomass and biofilm thickness were $30-42mg/cm^3$, $1.2-2.7mg/cm^2$ and $380-690{\mu}m$, respectively. These were greatly affected by the variation of temperature ($5-20^{\circ}C$) and packing ratio(45-90%). 2. The ratio of suspended biomass to the total biomass in the reactor was in the range of 10 to 50 % in accordance with the variation of temperature and packing ratio. Therefore, the portion of suspended biomass cannot be neglected. 3. BOD removal efficiency increased as either temperature or biomass(suspended and attached) concentration increased. 4. The aerated submerged biofilm process appeared to be less affected by temperature variation and the estimated temperature correction coefficient of the Van't Hoff-Arrhenenius equation was 1.042.

• Proceedings of the Membrane Society of Korea Conference
• /
• 1993.10a
• /
• pp.36-38
• /
• 1993

염료제조공정에서 발생되는 폐수는 미반응 물질, 중간합성체, 반응기 세척액 등의 난분해성 물질과 함께 고농도의 염(salt)를 함유하고 있어 기존의 생물학적 처리를 위해서는 폐수를 3-5배 희석하여 처리한다. 따라서 폐수처리장의 규모가 커지고 처리 약품과 에너지의 소모가 증가하므로 처리가 비효율적으로 수행된다. 본 연구에서는 염료폐수처리 및 회수에 역삼투막을 적용하여 폐수의 농축과정에 따른 투과율, 배제율의 변화를 조사하였다. 염료폐수의 생물학적 처리공정에서 문제점으로 대두되는 염의 농도를 낮추기 위해서 Nanofiltration(NF)막을 이용하여 염료성분과 염을 분리하는 실험을 수행하였다.